Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005966.1

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

66942 .. 76175

Gene Structure 9234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4224 bp

atgcggaggatcgctgtggcagcgtcccttgcttggctagattggggtttggatggattgggctcgcctgcttccggcttcgtcaccttcaccgtcacgtcgtcttcatcatccgctaaaggtttgatggattcataccatttgaatggcggagtggatgctaacgggatggatgatatcaaccggcctctgttggagcccttggatgatagggccgcagataagagaattagaacacttaagttcaaaattggggaaatccactgtgcttcttgttcaactaccattgaatctgtggtgggaagccttaatggggtcaacagcgttacggtgtcacccattcatggccaagctgctatcgattatataccagagctcattaatggaagcaaaatcaaggaagccatagaagatgcaggtttcccagttgatgaatttccagaacaagacattgcagtgtgccggctcaggataaaaggaatgatgtgcaccagttgctccgagtccgttgaatctgctcttcgaatggttgatggagtgaaaaacgcagttgttggtctagcccttgaggaagcaaaggttcactttgatccaaacatcactgacacctgttcgatcatgaaagcaatggcggagtggatgctaacgggatggatgatatctaccggcctctgttggagcccttggatgttagagccgcagataagagaattagaacacttaagttcaaaattggggaaattcactgtgcttcttgttcaactaccattgaatctgtggtgggaagccttaaaggggtcaagagcgttacggtgtcacccattcatggccaagctgctatcgattatataccagagctcattaatcaaaatcaaggaagccatagaagatgcaggtgtcccagttgatgaatttccagaacaagacattgcagtatgccggctcaggataaaaggaatgatgtgcaccagttgctccgagtccgttgaatctgctcttcgaatggttgatggagtgaaaaatgcagttgttggtctagcccttgaggaagcaaaggttcactttgatccaaacatcactgacacctgttcgatcatgaaagcaatagaagatgctggctttggatctgagctcattagctcagggaatgatgtaaacaaagtgcatctaagacttgaaggagttaattctcctgaagacatgaccatcatccaatcctcccttgaatcagttgagggtgtaaataatgttgaagtggacgtgttagaaaaaaaggtcaccctcacctacgatgcagacctcactggtccaagatctcttatacattgtattgaagaagctggataccaagcaagcttgtatgttcctccaaggcgaagagaagctgaacagcagcttgagactcagatcaaaatcaaggaagccatagaagatgcaggtgtcccagttgatgaatttccagaacaagacattgcagtatgccggctcaggataaaaggaatgatgtgcaccagttgctccgagtccgttgaatctgctcttcgaatggttgatggagtgaaaaatgcagttgttggtctagcccttgaggaagcaaaggttcactttgatccaaacatcactgacacctgttcgatcatgaaagcaatagaagatgctggctttggatctgagctcattagctcagggaatgatgtaaacaaagtgcatctaagacttgaaggagttaattctcctgaagacatgaccatcatccaatcctcccttgaatcagttgagggtgtaaataatgttgaagtggacgtgttagaaaaaaaggtcaccctcacctacgatgcagacctcactggtccaagatctcttatacattgtattgaagaagctggataccaagcaagcttgtatgttcctccaaggcgaagagaagctgaacagcagcttgagactcagatgtacaggaaccagtttttcttgagctgtctattttcagttcctgtatttttattctcaatggtgcttccaatgctttctccctatggggactggttaatgtacaagattcacaacatgctcactgtaggaatgcttttaagatggattctgtgcacacccgtgcagttcattattggcaggaggttttatgtgggatcatatcatgctttaagacgtaggtcagctaatatggatgttcttgttgcactgggcaccaatgttgcttatttctactctgtctatatagcaatgaaagcattggctttggataattttgagggggaagatttctttgaaactagttccatgttgatatcctttatactcttggggaagtacttagaagttctagctagaggaaaaacatctgatgctttagcaaagctgacagaccttactcctgaaactgcttatctgctatcactagatgctgatggcaatgctatctcagagatggaaattagcactcaacttattcaaagaaatgatatacttaagattgttcccggggcaaaagttcctgttgatgggattgtaattagtggtcaaagccatgtgaacgagagtatgatcacaggagaggcaaggcctatttctaaaagattaggtgacaaggtaattggtggcaccatgaatgagaatggatgcttacaggttaaggcaacccatgttggttcagagactgcgctatcccagatagttcaacttgttgaagctgcacagctttccagagcacctgttcagaagctagctgatcagatctcaaagtttttcgttccagcagttgtgattgcagcatttctgacatggctgggttggttcatcctgggagaattcggtctttatcccatatcatggacaccaaaaggcatggataggtttgagctggcactgcagttcggcatctcagtactagtggttgcatgcccttgtgctttgggattagcaacacctacagcagtcatggttgcaacagggaagggtgctacacaaggtgttcttatcaagggtggaaatgcacttgaaaaggctcataaggtgaaaacagttgtctttgataagacagggacactgacagttggaaaaccgacagtagttagtgctgttctattttccaattactcaatggaggaattctgtactgtggctactgctgcagaggcaaatagtgaacacccaatagcaaagtctattgtggagcatgcaaaggggtttcttaacaagtttgggtcaactgaacatgtggtggaggctaaggactttgaggtacacactggagctggtgttagtggaagagttggtgacaaaatggttttggttgggaacaagaggctcatgcgggaatataatgtccaggttggtcctgaggttgagaaattcatttcagaaaatgagaaactggctcgaacatgtgtgttagtttccattgatggaaaggttgctgggtcgtttgctgtaactgatccagtgaagccagaggctgcatgtgtaatatcttatctccactcaatgggcatttcaagcatcatggttactggtgataattgggcaacagcagcagcaattgcaagggaggttggcatcgatatcgataaggtgtttgctgagaccgatcccatgggaaaagccgatagaatcaaagagttacagatgaaaggattgactgtgactatggtgggagatggaatcaatgactcaccagctttggctgcagcagatgttggcatggcaattggcgctggcaccgatgtagcaattgaagctgctgatatagttttgatgaaaagcaatttggaagatgtagttacagccatagatctctctagaaagaccatgtctcgaatttggttgaattatgtctgggccctgggatacaacatccttggcatgccagttgctgcgggaatcttgttccctttcactgggatccgattaccaccttggcttgccggtgcctgtatggctgcttcatctgttagtgtgttggcttctactcttctcgctcttgccgacactgatgagactcttgatgttcagcatgcaaccagaaatctgtcaacaaatggtgagttatcgactccggcaaagacttttgacaatcaagaagcgcatattctgggcaaggctaataacattttcgactccggcaaagaacttttgacaatccagaagcgcatattctgggcaaggctaataacatttgggttttcatccatatacttccaacaatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1407

Amino Acids

152.81

Weight (kDa)

5.06

Isoelectric Point (pI)

40.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	82 - 141	7.4e-12	Heavy-metal-associated domain
HMA	PF00403	156 - 202	1.7e-06	Heavy-metal-associated domain
HMA	PF00403	315 - 374	1.4e-08	Heavy-metal-associated domain
HMA	PF00403	404 - 449	4.3e-07	Heavy-metal-associated domain
HMA	PF00403	496 - 555	1.4e-08	Heavy-metal-associated domain
HMA	PF00403	585 - 630	4.3e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	798 - 902	1.3e-24	P-type ATPase actuator domain
Hydrolase	PF00702	1011 - 1237	3.8e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	884, 1427
Acc36I	ACCTGC	3 cut(s)	407, 884, 1427
AccB1I	GGYRCC	4 cut(s)	2204, 2618, 3734, 3944
AccB7I	CCANNNNNTGG	2 cut(s)	2847, 3502
AciI	CCGC	8 cut(s)	4, 114, 150, 216, 632, 698, 3331, 3893
AcoI	YGGCCR	2 cut(s)	346, 828
AcsI	RAATTY	8 cut(s)	434, 740, 911, 1454, 2825, 3125, 3368, 3834
AcuI	CTGAAG	2 cut(s)	1215, 1758
AdeI	CACNNNGTG	1 cut(s)	2987
AfaI	GTAC	6 cut(s)	1955, 2054, 2330, 2904, 3133, 3261
AflII	CTTAAG	3 cut(s)	239, 721, 2486
AflIII	ACRYGT	4 cut(s)	1260, 1803, 3232, 3401
AhlI	ACTAGT	2 cut(s)	2291, 2905
AjiI	CACGTC	3 cut(s)	99, 1261, 1804
AjnI	CCWGG	3 cut(s)	2817, 3345, 3858
AjuI	GAANNNNNNNTTGG	2 cut(s)	3767, 3799
AleI	CACNNNNGTG	1 cut(s)	2111
Alw21I	GWGCWC	9 cut(s)	378, 491, 860, 968, 1134, 1511, 1677, 2108, 2728
Alw26I	GTCTC	6 cut(s)	1394, 1937, 2666, 3593, 3834, 4007
Alw44I	GTGCAC	4 cut(s)	487, 964, 1507, 2104
AlwNI	CAGNNNCTG	4 cut(s)	1994, 2675, 3473, 3890
Ama87I	CYCGRG	1 cut(s)	2498
AoxI	GGCC	7 cut(s)	185, 213, 346, 667, 828, 2579, 3855
ApaI	GGGCCC	1 cut(s)	3859
ApaLI	GTGCAC	4 cut(s)	487, 964, 1507, 2104
ApoI	RAATTY	8 cut(s)	434, 740, 911, 1454, 2825, 3125, 3368, 3834
AseI	ATTAAT	2 cut(s)	381, 863
AspLEI	GCGC	4 cut(s)	2680, 3731, 4102, 4167
AspS9I	GGNCC	6 cut(s)	213, 1307, 1850, 3353, 3855, 3856
AsuC2I	CCSGG	2 cut(s)	2499, 2500
AsuII	TTCGAA	3 cut(s)	523, 1000, 1543
AsuNHI	GCTAGC	1 cut(s)	2740
AvaI	CYCGRG	1 cut(s)	2498
AvaII	GGWCC	3 cut(s)	1307, 1850, 3353
AxyI	CCTNAGG	1 cut(s)	3357
BaeGI	GKGCMC	6 cut(s)	491, 968, 1511, 2108, 2207, 3859
BalI	TGGCCA	2 cut(s)	348, 830
BamHI	GGATCC	1 cut(s)	3918
BanI	GGYRCC	4 cut(s)	2204, 2618, 3734, 3944
BanII	GRGCYC	8 cut(s)	65, 201, 378, 683, 860, 1134, 1677, 3859
BarI	GAAGNNNNNNTAC	2 cut(s)	3943, 3975
BbsI	GAAGAC	3 cut(s)	96, 1203, 1746
Bbv12I	GWGCWC	9 cut(s)	378, 491, 860, 968, 1134, 1511, 1677, 2108, 2728
BcgI	CGANNNNNNTGC	2 cut(s)	3562, 3596
BciT130I	CCWGG	3 cut(s)	2819, 3347, 3860
BciVI	GTATCC	3 cut(s)	1337, 1880, 3857
BclI	TGATCA	2 cut(s)	2562, 2746
BcnI	CCSGG	2 cut(s)	2499, 2500
BcoDI	GTCTC	6 cut(s)	1394, 1937, 2666, 3593, 3834, 4007
BcuI	ACTAGT	2 cut(s)	2291, 2905
BfmI	CTRYAG	5 cut(s)	2076, 2885, 2946, 3147, 3702
BfoI	RGCGCY	1 cut(s)	3732
BfrI	CTTAAG	3 cut(s)	239, 721, 2486
BfuAI	ACCTGC	3 cut(s)	407, 884, 1427
BfuI	GTATCC	3 cut(s)	1337, 1880, 3857
BglI	GCCNNNNNGGC	1 cut(s)	3953
BglII	AGATCT	4 cut(s)	1313, 1856, 2751, 3808
BmcAI	AGTACT	2 cut(s)	2330, 2904
Bme1390I	CCNGG	5 cut(s)	2499, 2500, 2819, 3347, 3860
Bme18I	GGWCC	3 cut(s)	1307, 1850, 3353
BmeT110I	CYCGRG	1 cut(s)	2498
BmgBI	CACGTC	3 cut(s)	99, 1261, 1804
BmgT120I	GGNCC	6 cut(s)	213, 1307, 1850, 3353, 3855, 3856
BmiI	GGNNCC	9 cut(s)	198, 680, 1961, 2206, 2620, 3736, 3857, 3920, 3946
BmrFI	CCNGG	5 cut(s)	2499, 2500, 2819, 3347, 3860
BmrI	ACTGGG	5 cut(s)	419, 896, 1439, 2210, 3924
BmtI	GCTAGC	1 cut(s)	2744
BmuI	ACTGGG	5 cut(s)	419, 896, 1439, 2210, 3924
BoxI	GACNNNNGTC	1 cut(s)	2170
BpiI	GAAGAC	3 cut(s)	96, 1203, 1746
BpmI	CTGGAG	1 cut(s)	3288
Bpu10I	CCTNAGC	6 cut(s)	467, 944, 1141, 1487, 1684, 3246
Bpu14I	TTCGAA	3 cut(s)	523, 1000, 1543
BpuEI	CTTGAG	6 cut(s)	587, 1064, 1418, 1607, 1961, 1993
BpuMI	CCSGG	2 cut(s)	2499, 2500
Bsa29I	ATCGAT	4 cut(s)	360, 842, 3576, 3582
BsaBI	GATNNNNATC	1 cut(s)	1412
BsaI	GGTCTC	1 cut(s)	3593
BsaXI	ACNNNNNCTCC	2 cut(s)	3261, 3291
Bse118I	RCCGGY	6 cut(s)	183, 462, 665, 939, 1482, 3941
Bse21I	CCTNAGG	1 cut(s)	3357
Bse3DI	GCAATG	6 cut(s)	450, 633, 927, 1470, 2247, 2437
Bse8I	GATNNNNATC	1 cut(s)	1412
BseBI	CCWGG	3 cut(s)	2819, 3347, 3860
BseCI	ATCGAT	4 cut(s)	360, 842, 3576, 3582
BseJI	GATNNNNATC	1 cut(s)	1412
BseMI	GCAATG	6 cut(s)	450, 633, 927, 1470, 2247, 2437
BseSI	GKGCMC	6 cut(s)	491, 968, 1511, 2108, 2207, 3859
BseYI	CCCAGC	2 cut(s)	2803, 3434
BsgI	GTGCAG	2 cut(s)	2135, 2694
BshFI	GGCC	7 cut(s)	187, 215, 348, 669, 830, 2581, 3857
BshNI	GGYRCC	4 cut(s)	2204, 2618, 3734, 3944
BshVI	ATCGAT	4 cut(s)	360, 842, 3576, 3582
BsiHKAI	GWGCWC	9 cut(s)	378, 491, 860, 968, 1134, 1511, 1677, 2108, 2728
BsiHKCI	CYCGRG	1 cut(s)	2498
BslFI	GGGAC	5 cut(s)	10, 884, 1427, 2054, 3073
BsmAI	GTCTC	6 cut(s)	1394, 1937, 2666, 3593, 3834, 4007
BsmFI	GGGAC	5 cut(s)	10, 884, 1427, 2054, 3073
BsmI	GAATGC	1 cut(s)	2088
BsnI	GGCC	7 cut(s)	187, 215, 348, 669, 830, 2581, 3857
Bso31I	GGTCTC	1 cut(s)	3593
BsoBI	CYCGRG	1 cut(s)	2498
Bsp119I	TTCGAA	3 cut(s)	523, 1000, 1543
Bsp120I	GGGCCC	1 cut(s)	3855
Bsp1407I	TGTACA	2 cut(s)	1953, 2052
Bsp19I	CCATGG	1 cut(s)	3609
BspACI	CCGC	8 cut(s)	4, 114, 150, 216, 632, 698, 3331, 3893
BspANI	GGCC	7 cut(s)	187, 215, 348, 669, 830, 2581, 3857
BspDI	ATCGAT	4 cut(s)	360, 842, 3576, 3582
BspHI	TCATGA	3 cut(s)	618, 1095, 1638
BspLI	GGNNCC	9 cut(s)	198, 680, 1961, 2206, 2620, 3736, 3857, 3920, 3946
BspMAI	CTGCAG	3 cut(s)	2889, 3151, 3706
BspMI	ACCTGC	3 cut(s)	407, 884, 1427
BspOI	GCTAGC	1 cut(s)	2744
BspQI	GCTCTTC	3 cut(s)	525, 1002, 1545
BspT104I	TTCGAA	3 cut(s)	523, 1000, 1543
BspT107I	GGYRCC	4 cut(s)	2204, 2618, 3734, 3944
BspTI	CTTAAG	3 cut(s)	239, 721, 2486
BspTNI	GGTCTC	1 cut(s)	3593
BsrDI	GCAATG	6 cut(s)	450, 633, 927, 1470, 2247, 2437
BsrFI	RCCGGY	6 cut(s)	183, 462, 665, 939, 1482, 3941
BsrGI	TGTACA	2 cut(s)	1953, 2052
BssAI	RCCGGY	6 cut(s)	183, 462, 665, 939, 1482, 3941
BssT1I	CCWWGG	7 cut(s)	201, 683, 1370, 1913, 3609, 3874, 3932
Bst2UI	CCWGG	3 cut(s)	2819, 3347, 3860
Bst6I	CTCTTC	7 cut(s)	525, 1002, 1372, 1545, 1915, 3280, 3993
BstAFI	CTTAAG	3 cut(s)	239, 721, 2486
BstAPI	GCANNNNNTGC	4 cut(s)	495, 972, 1515, 3557
BstAUI	TGTACA	2 cut(s)	1953, 2052
BstBI	TTCGAA	3 cut(s)	523, 1000, 1543
BstDSI	CCRYGG	1 cut(s)	3609
BstEII	GGTNACC	2 cut(s)	1276, 1819
BstH2I	RGCGCY	1 cut(s)	3732
BstHHI	GCGC	4 cut(s)	2680, 3731, 4102, 4167
BstMAI	GTCTC	6 cut(s)	1394, 1937, 2666, 3593, 3834, 4007
BstNI	CCWGG	3 cut(s)	2819, 3347, 3860
BstNSI	RCATGY	8 cut(s)	2071, 2919, 3197, 3236, 3405, 3480, 3883, 4034
BstPAI	GACNNNNGTC	1 cut(s)	2170
BstPI	GGTNACC	2 cut(s)	1276, 1819
BstSCI	CCNGG	5 cut(s)	2497, 2498, 2817, 3345, 3858
BstSFI	CTRYAG	5 cut(s)	2076, 2885, 2946, 3147, 3702
BstSLI	GKGCMC	6 cut(s)	491, 968, 1511, 2108, 2207, 3859
BstV2I	GAAGAC	3 cut(s)	96, 1203, 1746
BstX2I	RGATCY	7 cut(s)	1124, 1313, 1667, 1856, 2751, 3808, 3918
BstXI	CCANNNNNNTGG	1 cut(s)	146
BstYI	RGATCY	7 cut(s)	1124, 1313, 1667, 1856, 2751, 3808, 3918
Bsu15I	ATCGAT	4 cut(s)	360, 842, 3576, 3582
Bsu36I	CCTNAGG	1 cut(s)	3357
BsuI	GTATCC	3 cut(s)	1337, 1880, 3857
BsuRI	GGCC	7 cut(s)	187, 215, 348, 669, 830, 2581, 3857
BsuTUI	ATCGAT	4 cut(s)	360, 842, 3576, 3582
BtgI	CCRYGG	1 cut(s)	3609
BtrI	CACGTC	3 cut(s)	99, 1261, 1804
BtsI	GCAGTG	2 cut(s)	462, 2882
BveI	ACCTGC	3 cut(s)	407, 884, 1427
CaiI	CAGNNNCTG	4 cut(s)	1994, 2675, 3473, 3890
CciI	TCATGA	3 cut(s)	618, 1095, 1638
CfoI	GCGC	4 cut(s)	2680, 3731, 4102, 4167
Cfr10I	RCCGGY	6 cut(s)	183, 462, 665, 939, 1482, 3941
Cfr13I	GGNCC	6 cut(s)	213, 1307, 1850, 3353, 3855, 3856
Cfr9I	CCCGGG	1 cut(s)	2498
ClaI	ATCGAT	4 cut(s)	360, 842, 3576, 3582
CseI	GACGC	1 cut(s)	12
Csp6I	GTAC	6 cut(s)	1954, 2053, 2329, 2903, 3132, 3260
CviQI	GTAC	6 cut(s)	1954, 2053, 2329, 2903, 3132, 3260
DraIII	CACNNNGTG	1 cut(s)	2987
EaeI	YGGCCR	2 cut(s)	346, 828
Eam1104I	CTCTTC	7 cut(s)	525, 1002, 1372, 1545, 1915, 3280, 3993
EarI	CTCTTC	7 cut(s)	525, 1002, 1372, 1545, 1915, 3280, 3993
EciI	GGCGGA	2 cut(s)	165, 647
Ecl136II	GAGCTC	4 cut(s)	376, 858, 1132, 1675
Eco130I	CCWWGG	7 cut(s)	201, 683, 1370, 1913, 3609, 3874, 3932
Eco147I	AGGCCT	1 cut(s)	2581
Eco24I	GRGCYC	8 cut(s)	65, 201, 378, 683, 860, 1134, 1677, 3859
Eco31I	GGTCTC	1 cut(s)	3593
Eco32I	GATATC	4 cut(s)	178, 660, 2307, 3580
Eco47I	GGWCC	3 cut(s)	1307, 1850, 3353
Eco53kI	GAGCTC	4 cut(s)	376, 858, 1132, 1675
Eco57I	CTGAAG	2 cut(s)	1215, 1758
Eco81I	CCTNAGG	1 cut(s)	3357
Eco88I	CYCGRG	1 cut(s)	2498
Eco91I	GGTNACC	2 cut(s)	1276, 1819
EcoICRI	GAGCTC	4 cut(s)	376, 858, 1132, 1675
EcoO109I	RGGNCCY	1 cut(s)	3856
EcoO65I	GGTNACC	2 cut(s)	1276, 1819
EcoRI	GAATTC	2 cut(s)	2825, 3125
EcoRII	CCWGG	3 cut(s)	2817, 3345, 3858
EcoRV	GATATC	4 cut(s)	178, 660, 2307, 3580
EcoT14I	CCWWGG	7 cut(s)	201, 683, 1370, 1913, 3609, 3874, 3932
EcoT38I	GRGCYC	8 cut(s)	65, 201, 378, 683, 860, 1134, 1677, 3859
ErhI	CCWWGG	7 cut(s)	201, 683, 1370, 1913, 3609, 3874, 3932
FalI	AAGNNNNNCTT	8 cut(s)	569, 601, 1046, 1078, 1589, 1621, 2976, 3008
FaqI	GGGAC	5 cut(s)	10, 884, 1427, 2054, 3073
FauI	CCCGC	2 cut(s)	3324, 3886
FbaI	TGATCA	2 cut(s)	2562, 2746
FriOI	GRGCYC	8 cut(s)	65, 201, 378, 683, 860, 1134, 1677, 3859
GlaI	GCGC	4 cut(s)	2679, 3730, 4101, 4166
GsaI	CCCAGC	2 cut(s)	2807, 3438
GsuI	CTGGAG	1 cut(s)	3288
HaeII	RGCGCY	1 cut(s)	3732
HaeIII	GGCC	7 cut(s)	187, 215, 348, 669, 830, 2581, 3857
HgaI	GACGC	1 cut(s)	12
HhaI	GCGC	4 cut(s)	2680, 3731, 4102, 4167
Hin6I	GCGC	4 cut(s)	2678, 3729, 4100, 4165
HinP1I	GCGC	4 cut(s)	2678, 3729, 4100, 4165
HincII	GTYRAC	3 cut(s)	319, 3225, 4050
HindII	GTYRAC	3 cut(s)	319, 3225, 4050
HindIII	AAGCTT	2 cut(s)	1354, 1897
Hpy99I	CGWCG	1 cut(s)	103
HpyAV	CCTTC	4 cut(s)	97, 1172, 1715, 2965
HpyCH4IV	ACGT	4 cut(s)	98, 1260, 1803, 2167
HpySE526I	ACGT	4 cut(s)	98, 1260, 1803, 2167
HspAI	GCGC	4 cut(s)	2678, 3729, 4100, 4165
KroI	GCCGGC	3 cut(s)	462, 939, 1482
KroNI	GCCGGC	3 cut(s)	464, 941, 1484
Ksp22I	TGATCA	2 cut(s)	2562, 2746
LguI	GCTCTTC	3 cut(s)	525, 1002, 1545
LmnI	GCTCC	7 cut(s)	196, 503, 678, 980, 1523, 3190, 3269
MaeII	ACGT	4 cut(s)	98, 1260, 1803, 2167
MfeI	CAATTG	3 cut(s)	3555, 3723, 3747
MflI	RGATCY	7 cut(s)	1124, 1313, 1667, 1856, 2751, 3808, 3918
MlsI	TGGCCA	2 cut(s)	348, 830
MluNI	TGGCCA	2 cut(s)	348, 830
MlyI	GAGTC	9 cut(s)	512, 989, 1396, 1532, 1939, 3680, 4009, 4063, 4127
MmeI	TCCRAC	3 cut(s)	174, 656, 3052
Mox20I	TGGCCA	2 cut(s)	348, 830
MroNI	GCCGGC	3 cut(s)	462, 939, 1482
MscI	TGGCCA	2 cut(s)	348, 830
MslI	CAYNNNNRTG	3 cut(s)	144, 455, 2111
Msp20I	TGGCCA	2 cut(s)	348, 830
MspCI	CTTAAG	3 cut(s)	239, 721, 2486
MspR9I	CCNGG	5 cut(s)	2499, 2500, 2819, 3347, 3860
MunI	CAATTG	3 cut(s)	3555, 3723, 3747
Mva1269I	GAATGC	1 cut(s)	2088
MvaI	CCWGG	3 cut(s)	2819, 3347, 3860
NaeI	GCCGGC	3 cut(s)	464, 941, 1484
NciI	CCSGG	2 cut(s)	2499, 2500
NcoI	CCATGG	1 cut(s)	3609
NgoMIV	GCCGGC	3 cut(s)	462, 939, 1482
NheI	GCTAGC	1 cut(s)	2740
NlaIV	GGNNCC	9 cut(s)	198, 680, 1961, 2206, 2620, 3736, 3857, 3920, 3946
NmuCI	GTSAC	9 cut(s)	82, 94, 333, 815, 1276, 1819, 2600, 3293, 3658
NspI	RCATGY	8 cut(s)	2071, 2919, 3197, 3236, 3405, 3480, 3883, 4034
NspV	TTCGAA	3 cut(s)	523, 1000, 1543
OliI	CACNNNNGTG	1 cut(s)	2111
PaeI	GCATGC	4 cut(s)	2919, 3197, 3883, 4034
PagI	TCATGA	3 cut(s)	618, 1095, 1638
PaqCI	CACCTGC	2 cut(s)	884, 1427
PasI	CCCWGGG	1 cut(s)	3859
PceI	AGGCCT	1 cut(s)	2581
PciI	ACATGT	2 cut(s)	3232, 3401
PciSI	GCTCTTC	3 cut(s)	525, 1002, 1545
PcsI	WCGNNNNNNNCGW	1 cut(s)	3999
PctI	GAATGC	1 cut(s)	2088
PdiI	GCCGGC	3 cut(s)	464, 941, 1484
PflFI	GACNNNGTC	1 cut(s)	3826
PflMI	CCANNNNNTGG	2 cut(s)	2847, 3502
PleI	GAGTC	9 cut(s)	511, 988, 1396, 1531, 1939, 3680, 4009, 4063, 4127
PpsI	GAGTC	9 cut(s)	511, 988, 1396, 1531, 1939, 3680, 4009, 4063, 4127
PscI	ACATGT	2 cut(s)	3232, 3401
PshAI	GACNNNNGTC	1 cut(s)	2170
PshBI	ATTAAT	2 cut(s)	381, 863
Psp124BI	GAGCTC	4 cut(s)	378, 860, 1134, 1677
Psp6I	CCWGG	3 cut(s)	2817, 3345, 3858
PspEI	GGTNACC	2 cut(s)	1276, 1819
PspFI	CCCAGC	2 cut(s)	2803, 3434
PspGI	CCWGG	3 cut(s)	2817, 3345, 3858
PspN4I	GGNNCC	9 cut(s)	198, 680, 1961, 2206, 2620, 3736, 3857, 3920, 3946
PspOMI	GGGCCC	1 cut(s)	3855
PspPI	GGNCC	6 cut(s)	213, 1307, 1850, 3353, 3855, 3856
PstI	CTGCAG	3 cut(s)	2889, 3151, 3706
PstNI	CAGNNNCTG	4 cut(s)	1994, 2675, 3473, 3890
PsuI	RGATCY	7 cut(s)	1124, 1313, 1667, 1856, 2751, 3808, 3918
PsyI	GACNNNGTC	1 cut(s)	3826
RsaI	GTAC	6 cut(s)	1955, 2054, 2330, 2904, 3133, 3261
RsaNI	GTAC	6 cut(s)	1954, 2053, 2329, 2903, 3132, 3260
RseI	CAYNNNNRTG	3 cut(s)	144, 455, 2111
SacI	GAGCTC	4 cut(s)	378, 860, 1134, 1677
SapI	GCTCTTC	3 cut(s)	525, 1002, 1545
Sau96I	GGNCC	6 cut(s)	213, 1307, 1850, 3353, 3855, 3856
ScaI	AGTACT	2 cut(s)	2330, 2904
SchI	GAGTC	9 cut(s)	512, 989, 1396, 1532, 1939, 3680, 4009, 4063, 4127
ScrFI	CCNGG	5 cut(s)	2499, 2500, 2819, 3347, 3860
SfcI	CTRYAG	5 cut(s)	2076, 2885, 2946, 3147, 3702
SfuI	TTCGAA	3 cut(s)	523, 1000, 1543
SinI	GGWCC	3 cut(s)	1307, 1850, 3353
SmaI	CCCGGG	1 cut(s)	2500
SmiMI	CAYNNNNRTG	3 cut(s)	144, 455, 2111
SmlI	CTYRAG	9 cut(s)	239, 566, 721, 1043, 1397, 1586, 1940, 1972, 2486
SmoI	CTYRAG	9 cut(s)	239, 566, 721, 1043, 1397, 1586, 1940, 1972, 2486
SpeI	ACTAGT	2 cut(s)	2291, 2905
SphI	GCATGC	4 cut(s)	2919, 3197, 3883, 4034
SseBI	AGGCCT	1 cut(s)	2581
SsiI	CCGC	8 cut(s)	4, 114, 150, 216, 632, 698, 3331, 3893
SstI	GAGCTC	4 cut(s)	378, 860, 1134, 1677
StuI	AGGCCT	1 cut(s)	2581
StyD4I	CCNGG	5 cut(s)	2497, 2498, 2817, 3345, 3858
StyI	CCWWGG	7 cut(s)	201, 683, 1370, 1913, 3609, 3874, 3932
TaiI	ACGT	4 cut(s)	101, 1263, 1806, 2170
TaqII	GACCGA	2 cut(s)	2819, 3617
TatI	WGTACW	5 cut(s)	1953, 2052, 2328, 2902, 3131
TauI	GCSGC	2 cut(s)	218, 700
TseFI	GTSAC	9 cut(s)	82, 94, 333, 815, 1276, 1819, 2600, 3293, 3658
Tsp45I	GTSAC	9 cut(s)	82, 94, 333, 815, 1276, 1819, 2600, 3293, 3658
TspGWI	ACGGA	3 cut(s)	496, 973, 1516
TspMI	CCCGGG	1 cut(s)	2498
Tth111I	GACNNNGTC	1 cut(s)	3826
Van91I	CCANNNNNTGG	2 cut(s)	2847, 3502
Vha464I	CTTAAG	3 cut(s)	239, 721, 2486
VneI	GTGCAC	4 cut(s)	487, 964, 1507, 2104
VpaK11BI	GGWCC	3 cut(s)	1307, 1850, 3353
VspI	ATTAAT	2 cut(s)	381, 863
XapI	RAATTY	8 cut(s)	434, 740, 911, 1454, 2825, 3125, 3368, 3834
XbaI	TCTAGA	1 cut(s)	3815
XceI	RCATGY	8 cut(s)	2071, 2919, 3197, 3236, 3405, 3480, 3883, 4034
XcmI	CCANNNNNNNNNTGG	3 cut(s)	292, 774, 3115
XmaI	CCCGGG	1 cut(s)	2498
ZrmI	AGTACT	2 cut(s)	2330, 2904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.