Rmu_sc0006065.1_g000008

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006065.1
OG0016946
Physical Location & Seq
Forward (+)
36984 .. 43885
6902 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
atgagaagatcgtggtttagtaatacgtcaccgtctctgattcttctcaagaagctgacatcttcgagcgtcgccgcctcatacaggataacaaagccagaattcaccgaatcaaattacagagtaagagaacggagaccggaagttccaagcttctggccgtcgaacatcatcttccggatccacgacctcagctcactaaatgttctcagctgcggcggcgtgacgctttggactcgcttgcgtttgaccattctaaagaagcatattgtggaggcaacttggcaaggccgggaaggttgcttcccggcgacggatttgcaacttccggataccagaaaccagaagcctcactacaagcattcggtttttgagaagagtagagaatcggatagatctgagttggaatccagtaaggtatctccgatcggagcttcgtcgtcgtctttaggcgtggctgcacctttttgtggttttgtaagctttgatagattggaatctcaccagatcggaggcggtggtcgtcggaattggattgggggacaaagagtgtcgtgccggtggtcgcctgaatcggatggaggagagagagagtcgtgttggtggtcaccggaatcggaggcggagaccaaagacatgaaaaatctagggtcgaggagagagagagagagagagtctgatggaattaattgggtttatgaagtaggaggagaccataaacgaaatcttgaagcggctatcacgctcgcgttgattgcaatcattgcatctgataattggattagtgaggacggagaaaacctaggccacggtggtcaccacctcacaactcctgtagtcttgaacccaactgatttctatcccatttttgtatttgttccatcaaaggtggacctccccagatttaagaatgaagaagtgaaggttgagctagaggaagggaatgtccttcatatcaatggagagagaagcagtgagcaagaggagaagaacgacaagtggcacagcgtagagaggagcagtggcaagttcttgaggaggttcaggctgccagtacgtcgtcgtctccacctcaccagagcctcgagttcatcgtcaagtcactgtgaggtagtggaggtcagagaggcaagtggttctggaaatgacgacggagggcatggtggtcatcccctatggaaaatggcgggaaatcaaggtctggaagagtctgggcctctccggagcaatccctctccttctcttacctgtgaccacactccagtcccagaatcctcaggagccgactgtcaatgccaccaaaccctctgtcaacatgctttggtcctagtctccttgggagcagactgtagtaccgcgaccacaacggttacggaaccagccaagcaaggaagcttagcaaaagttttcaccacgaggcacaaagaatcccgtgacaacgttggtgctttcctcatccacaatttcttgaaagaagtcaggtcaagggacacccccgacgaccgtacccgaacggtgttggcaagcccacgcagaaaagagactgttgaccagctgcaacaaaactggagccaaacagtcacgtttggttcgtggaaagtaagcatcaggaaaagaaatttgtttctttcatgtgtttgggatacaaatggaaagaaaatactttcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

556

Amino Acids

62.37

Weight (kDa)

8.64

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 296 - 352 3.1e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 749, 1358
AccIII TCCGGA 3 cut(s) 177, 328, 1221
AciI CCGC 8 cut(s) 75, 216, 219, 516, 623, 734, 1187, 1356
AclI AACGTT 1 cut(s) 1440
AclWI GGATC 2 cut(s) 175, 188
AcoI YGGCCR 1 cut(s) 158
AcsI RAATTY 2 cut(s) 101, 1618
AfaI GTAC 3 cut(s) 1056, 1354, 1507
AfiI CCNNNNNNNGG 3 cut(s) 84, 313, 470
AgsI TTSAA 3 cut(s) 731, 844, 1471
AluBI AGCT 9 cut(s) 55, 153, 195, 213, 434, 483, 931, 1395, 1555
AluI AGCT 9 cut(s) 55, 153, 195, 213, 434, 483, 931, 1395, 1555
Alw26I GTCTC 7 cut(s) 39, 130, 620, 705, 1070, 1336, 1535
AlwI GGATC 2 cut(s) 175, 188
Ama87I CYCGRG 1 cut(s) 1084
Aor13HI TCCGGA 3 cut(s) 177, 328, 1221
AoxI GGCC 4 cut(s) 158, 289, 805, 1214
ApeKI GCWGC 4 cut(s) 213, 458, 1048, 1555
ApoI RAATTY 2 cut(s) 101, 1618
ArsI GACNNNNNNTTYG 2 cut(s) 1294, 1326
AseI ATTAAT 1 cut(s) 687
AspA2I CCTAGG 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 892, 1214, 1324
AsuC2I CCSGG 2 cut(s) 293, 308
AsuHPI GGTGA 7 cut(s) 21, 97, 494, 600, 809, 1066, 1402
AvaI CYCGRG 1 cut(s) 1084
AvaII GGWCC 2 cut(s) 892, 1324
AvrII CCTAGG 1 cut(s) 802
AxyI CCTNAGG 1 cut(s) 1276
BaeI ACNNNNGTAYC 4 cut(s) 1336, 1369, 1489, 1522
BamHI GGATCC 1 cut(s) 180
BarI GAAGNNNNNNTAC 2 cut(s) 338, 370
BauI CACGAG 1 cut(s) 1414
BbvCI CCTCAGC 1 cut(s) 191
BbvI GCAGC 4 cut(s) 200, 445, 1035, 1542
BccI CCATC 3 cut(s) 572, 674, 889
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 2 cut(s) 325, 1636
BcnI CCSGG 2 cut(s) 293, 308
BcoDI GTCTC 7 cut(s) 39, 130, 620, 705, 1070, 1336, 1535
BfaI CTAG 4 cut(s) 647, 803, 932, 1328
BfmI CTRYAG 2 cut(s) 834, 1348
BfuI GTATCC 2 cut(s) 325, 1636
BglII AGATCT 1 cut(s) 395
BisI GCNGC 8 cut(s) 75, 214, 217, 220, 459, 735, 1049, 1556
BlnI CCTAGG 1 cut(s) 802
BlpI GCTNAGC 1 cut(s) 1396
BlsI GCNGC 8 cut(s) 76, 215, 218, 221, 460, 736, 1050, 1557
Bme1390I CCNGG 2 cut(s) 293, 308
Bme18I GGWCC 2 cut(s) 892, 1324
BmeT110I CYCGRG 1 cut(s) 1084
BmgT120I GGNCC 3 cut(s) 892, 1214, 1324
BmiI GGNNCC 4 cut(s) 182, 1282, 1377, 1571
BmrFI CCNGG 2 cut(s) 293, 308
BmsI GCATC 2 cut(s) 776, 1614
BpmI CTGGAG 2 cut(s) 1245, 1588
Bpu10I CCTNAGC 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 1396
BpuEI CTTGAG 2 cut(s) 32, 1054
BpuMI CCSGG 2 cut(s) 293, 308
BsaBI GATNNNNATC 3 cut(s) 496, 758, 858
BsaI GGTCTC 3 cut(s) 130, 620, 705
BsaJI CCNNGG 3 cut(s) 802, 808, 1335
BsaWI WCCGGW 5 cut(s) 139, 177, 328, 610, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 313, 470
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 4 cut(s) 411, 1052, 1262, 1571
Bse21I CCTNAGG 1 cut(s) 1276
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 3 cut(s) 496, 758, 858
BseAI TCCGGA 3 cut(s) 177, 328, 1221
BseDI CCNNGG 3 cut(s) 802, 808, 1335
BseGI GGATG 3 cut(s) 583, 1168, 1455
BseJI GATNNNNATC 3 cut(s) 496, 758, 858
BseLI CCNNNNNNNGG 3 cut(s) 84, 313, 470
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 4 cut(s) 205, 223, 390, 1290
BseNI ACTGG 4 cut(s) 411, 1052, 1262, 1571
BseRI GAGGAG 6 cut(s) 597, 670, 723, 998, 1030, 1051
BseXI GCAGC 4 cut(s) 200, 445, 1035, 1542
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 2 cut(s) 749, 1358
Bsh1285I CGRYCG 2 cut(s) 429, 1504
BshFI GGCC 4 cut(s) 160, 291, 807, 1216
BsiEI CGRYCG 2 cut(s) 429, 1504
BsiHKCI CYCGRG 1 cut(s) 1084
BsiSI CCGG 8 cut(s) 140, 178, 292, 308, 329, 559, 611, 1222
BslFI GGGAC 3 cut(s) 555, 1250, 1502
BslI CCNNNNNNNGG 3 cut(s) 84, 313, 470
BsmAI GTCTC 7 cut(s) 39, 130, 620, 705, 1070, 1336, 1535
BsmBI CGTCTC 2 cut(s) 39, 1070
BsmFI GGGAC 3 cut(s) 555, 1250, 1502
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 4 cut(s) 160, 291, 807, 1216
Bso31I GGTCTC 3 cut(s) 130, 620, 705
BsoBI CYCGRG 1 cut(s) 1084
Bsp13I TCCGGA 3 cut(s) 177, 328, 1221
Bsp143I GATC 5 cut(s) 8, 180, 395, 426, 507
Bsp1720I GCTNAGC 1 cut(s) 1396
BspACI CCGC 8 cut(s) 75, 216, 219, 516, 623, 734, 1187, 1356
BspANI GGCC 4 cut(s) 160, 291, 807, 1216
BspCNI CTCAG 4 cut(s) 204, 222, 391, 1289
BspEI TCCGGA 3 cut(s) 177, 328, 1221
BspFNI CGCG 2 cut(s) 749, 1358
BspLI GGNNCC 4 cut(s) 182, 1282, 1377, 1571
BspPI GGATC 2 cut(s) 175, 188
BspTNI GGTCTC 3 cut(s) 130, 620, 705
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 4 cut(s) 411, 1052, 1262, 1571
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 3 cut(s) 802, 808, 1335
BssMI GATC 5 cut(s) 8, 180, 395, 426, 507
BssSI CACGAG 1 cut(s) 1414
BssT1I CCWWGG 2 cut(s) 802, 1335
Bst2BI CACGAG 1 cut(s) 1414
Bst6I CTCTTC 2 cut(s) 371, 1200
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 242, 747, 1525
BstDEI CTNAG 5 cut(s) 191, 209, 399, 1276, 1396
BstDSI CCRYGG 1 cut(s) 808
BstEII GGTNACC 2 cut(s) 606, 815
BstENI CCTNNNNNAGG 1 cut(s) 82
BstF5I GGATG 3 cut(s) 583, 1168, 1455
BstFNI CGCG 2 cut(s) 749, 1358
BstKTI GATC 5 cut(s) 11, 183, 398, 429, 510
BstMAI GTCTC 7 cut(s) 39, 130, 620, 705, 1070, 1336, 1535
BstMBI GATC 5 cut(s) 8, 180, 395, 426, 507
BstMCI CGRYCG 2 cut(s) 429, 1504
BstMWI GCNNNNNNNGC 3 cut(s) 219, 755, 764
BstNSI RCATGY 1 cut(s) 1319
BstPI GGTNACC 2 cut(s) 606, 815
BstSCI CCNGG 2 cut(s) 291, 306
BstSFI CTRYAG 2 cut(s) 834, 1348
BstUI CGCG 2 cut(s) 749, 1358
BstV1I GCAGC 4 cut(s) 200, 445, 1035, 1542
BstX2I RGATCY 2 cut(s) 180, 395
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 2 cut(s) 180, 395
Bsu36I CCTNAGG 1 cut(s) 1276
BsuI GTATCC 2 cut(s) 325, 1636
BsuRI GGCC 4 cut(s) 160, 291, 807, 1216
BtgI CCRYGG 1 cut(s) 808
BtsCI GGATG 3 cut(s) 583, 1168, 1455
BtsI GCAGTG 2 cut(s) 979, 1027
BtsIMutI CAGTG 3 cut(s) 979, 1027, 1102
Cac8I GCNNGC 3 cut(s) 242, 747, 1525
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 3 cut(s) 892, 1214, 1324
CseI GACGC 2 cut(s) 58, 235
Csp6I GTAC 3 cut(s) 1055, 1353, 1506
CviAII CATG 4 cut(s) 637, 1160, 1316, 1632
CviQI GTAC 3 cut(s) 1055, 1353, 1506
DdeI CTNAG 5 cut(s) 191, 209, 399, 1276, 1396
DpnI GATC 5 cut(s) 10, 182, 397, 428, 509
DpnII GATC 5 cut(s) 8, 180, 395, 426, 507
EaeI YGGCCR 1 cut(s) 158
Eam1104I CTCTTC 2 cut(s) 371, 1200
EarI CTCTTC 2 cut(s) 371, 1200
EciI GGCGGA 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 802, 1335
Eco31I GGTCTC 3 cut(s) 130, 620, 705
Eco47I GGWCC 2 cut(s) 892, 1324
Eco81I CCTNAGG 1 cut(s) 1276
Eco88I CYCGRG 1 cut(s) 1084
Eco91I GGTNACC 2 cut(s) 606, 815
EcoNI CCTNNNNNAGG 1 cut(s) 82
EcoO65I GGTNACC 2 cut(s) 606, 815
EcoRI GAATTC 1 cut(s) 101
EcoT14I CCWWGG 2 cut(s) 802, 1335
ErhI CCWWGG 2 cut(s) 802, 1335
Esp3I CGTCTC 2 cut(s) 39, 1070
FaeI CATG 4 cut(s) 640, 1163, 1319, 1635
FaqI GGGAC 3 cut(s) 555, 1250, 1502
FatI CATG 4 cut(s) 636, 1159, 1315, 1631
FauI CCCGC 1 cut(s) 1180
Fnu4HI GCNGC 8 cut(s) 75, 214, 217, 220, 459, 735, 1049, 1556
FokI GGATG 3 cut(s) 590, 1155, 1442
Fsp4HI GCNGC 8 cut(s) 75, 214, 217, 220, 459, 735, 1049, 1556
FspBI CTAG 4 cut(s) 647, 803, 932, 1328
GluI GCNGC 8 cut(s) 75, 214, 217, 220, 459, 735, 1049, 1556
GsuI CTGGAG 2 cut(s) 1245, 1588
HaeIII GGCC 4 cut(s) 160, 291, 807, 1216
HapII CCGG 8 cut(s) 140, 178, 292, 308, 329, 559, 611, 1222
HgaI GACGC 2 cut(s) 58, 235
Hin1II CATG 4 cut(s) 640, 1163, 1319, 1635
HincII GTYRAC 2 cut(s) 1313, 1549
HindII GTYRAC 2 cut(s) 1313, 1549
HindIII AAGCTT 3 cut(s) 151, 481, 1393
HpaII CCGG 8 cut(s) 140, 178, 292, 308, 329, 559, 611, 1222
HphI GGTGA 7 cut(s) 21, 97, 494, 600, 809, 1066, 1402
Hpy166II GTNNAC 3 cut(s) 892, 1313, 1549
Hpy8I GTNNAC 3 cut(s) 892, 1313, 1549
HpyAV CCTTC 5 cut(s) 290, 916, 932, 959, 1248
HpyCH4IV ACGT 4 cut(s) 26, 1057, 1440, 1583
HpyCH4V TGCA 5 cut(s) 322, 461, 758, 767, 1558
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 755, 764
HpyF3I CTNAG 5 cut(s) 191, 209, 399, 1276, 1396
HpySE526I ACGT 4 cut(s) 26, 1057, 1440, 1583
Hsp92II CATG 4 cut(s) 640, 1163, 1319, 1635
Kpn2I TCCGGA 3 cut(s) 177, 328, 1221
Kzo9I GATC 5 cut(s) 8, 180, 395, 426, 507
LmnI GCTCC 6 cut(s) 431, 1017, 1224, 1280, 1340, 1569
Lsp1109I GCAGC 4 cut(s) 200, 445, 1035, 1542
LweI GCATC 2 cut(s) 776, 1614
MaeI CTAG 4 cut(s) 647, 803, 932, 1328
MaeII ACGT 4 cut(s) 26, 1057, 1440, 1583
MaeIII GTNAC 9 cut(s) 27, 223, 606, 815, 1100, 1250, 1369, 1433, 1579
MalI GATC 5 cut(s) 10, 182, 397, 428, 509
MboI GATC 5 cut(s) 8, 180, 395, 426, 507
MboII GAAGA 8 cut(s) 18, 35, 54, 166, 388, 926, 1000, 1217
MflI RGATCY 2 cut(s) 180, 395
MluCI AATT 8 cut(s) 101, 115, 529, 684, 688, 775, 1462, 1618
MlyI GAGTC 4 cut(s) 229, 602, 683, 1217
MmeI TCCRAC 2 cut(s) 384, 506
MroI TCCGGA 3 cut(s) 177, 328, 1221
MseI TTAA 2 cut(s) 687, 906
MslI CAYNNNNRTG 1 cut(s) 957
MspA1I CMGCKG 2 cut(s) 213, 1555
MspI CCGG 8 cut(s) 140, 178, 292, 308, 329, 559, 611, 1222
MspR9I CCNGG 2 cut(s) 293, 308
Mva1269I GAATGC 1 cut(s) 361
MvnI CGCG 2 cut(s) 749, 1358
MwoI GCNNNNNNNGC 3 cut(s) 219, 755, 764
NciI CCSGG 2 cut(s) 293, 308
NdeII GATC 5 cut(s) 8, 180, 395, 426, 507
NlaIII CATG 4 cut(s) 640, 1163, 1319, 1635
NlaIV GGNNCC 4 cut(s) 182, 1282, 1377, 1571
NmuCI GTSAC 8 cut(s) 27, 223, 606, 815, 1100, 1250, 1433, 1579
NspI RCATGY 1 cut(s) 1319
PaeR7I CTCGAG 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 2 cut(s) 1091, 1589
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 9 cut(s) 40, 110, 386, 407, 497, 572, 614, 1271, 1427
PkrI GCNGC 8 cut(s) 76, 215, 218, 221, 460, 736, 1050, 1557
Ple19I CGATCG 1 cut(s) 429
PleI GAGTC 4 cut(s) 229, 601, 682, 1216
PpsI GAGTC 4 cut(s) 229, 601, 682, 1216
PshBI ATTAAT 1 cut(s) 687
Psp1406I AACGTT 1 cut(s) 1440
PspEI GGTNACC 2 cut(s) 606, 815
PspN4I GGNNCC 4 cut(s) 182, 1282, 1377, 1571
PspPI GGNCC 3 cut(s) 892, 1214, 1324
PspXI VCTCGAGB 1 cut(s) 1084
PsuI RGATCY 2 cut(s) 180, 395
PvuI CGATCG 1 cut(s) 429
PvuII CAGCTG 2 cut(s) 213, 1555
RsaI GTAC 3 cut(s) 1056, 1354, 1507
RsaNI GTAC 3 cut(s) 1055, 1353, 1506
RseI CAYNNNNRTG 1 cut(s) 957
SaqAI TTAA 2 cut(s) 687, 906
SatI GCNGC 8 cut(s) 75, 214, 217, 220, 459, 735, 1049, 1556
Sau3AI GATC 5 cut(s) 8, 180, 395, 426, 507
Sau96I GGNCC 3 cut(s) 892, 1214, 1324
SchI GAGTC 4 cut(s) 229, 602, 683, 1217
ScrFI CCNGG 2 cut(s) 293, 308
SfaNI GCATC 2 cut(s) 776, 1614
SfcI CTRYAG 2 cut(s) 834, 1348
Sfr274I CTCGAG 1 cut(s) 1084
SinI GGWCC 2 cut(s) 892, 1324
SlaI CTCGAG 1 cut(s) 1084
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 3 cut(s) 47, 1033, 1084
SmoI CTYRAG 3 cut(s) 47, 1033, 1084
Sse9I AATT 8 cut(s) 101, 115, 529, 684, 688, 775, 1462, 1618
SsiI CCGC 8 cut(s) 75, 216, 219, 516, 623, 734, 1187, 1356
SspMI CTAG 4 cut(s) 647, 803, 932, 1328
StyD4I CCNGG 2 cut(s) 291, 306
StyI CCWWGG 2 cut(s) 802, 1335
TaiI ACGT 4 cut(s) 29, 1060, 1443, 1586
TaqI TCGA 4 cut(s) 65, 164, 653, 1085
TasI AATT 8 cut(s) 101, 115, 529, 684, 688, 775, 1462, 1618
TauI GCSGC 4 cut(s) 77, 219, 222, 737
TfiI GAWTC 9 cut(s) 40, 110, 386, 407, 497, 572, 614, 1271, 1427
Tru1I TTAA 2 cut(s) 687, 906
Tru9I TTAA 2 cut(s) 687, 906
TscAI CASTG 3 cut(s) 979, 1027, 1109
TseFI GTSAC 8 cut(s) 27, 223, 606, 815, 1100, 1250, 1433, 1579
TseI GCWGC 4 cut(s) 213, 458, 1048, 1555
Tsp45I GTSAC 8 cut(s) 27, 223, 606, 815, 1100, 1250, 1433, 1579
TspDTI ATGAA 6 cut(s) 653, 714, 927, 941, 1080, 1620
TspGWI ACGGA 5 cut(s) 148, 329, 807, 1167, 1388
TspRI CASTG 3 cut(s) 979, 1027, 1109
VpaK11BI GGWCC 2 cut(s) 892, 1324
VspI ATTAAT 1 cut(s) 687
XagI CCTNNNNNAGG 1 cut(s) 82
XapI RAATTY 2 cut(s) 101, 1618
XceI RCATGY 1 cut(s) 1319
XhoI CTCGAG 1 cut(s) 1084
XmaJI CCTAGG 1 cut(s) 802
XspI CTAG 4 cut(s) 647, 803, 932, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.