Rmu_sc0006261.1_g000001

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006261.1
OG0010359
Physical Location & Seq
Reverse (-)
4196 .. 6066
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
atgagtgtttatttgactgtgatgacgttcaatcttcatgaagatccgacagcagatagttctaattcatgggaaaagagaagagatttgtgtataagcgtcgttactagttattctccgatgattctctgtactcaacaaggagctaaatcacaattggattatcttcaacaatgcttgccaggttatgaccaatttgggatatcaagaaaaggacctgaagacacttcagacgaacattgcaccatcttctatgacaaggagaaggtggaggttattgaaggtggaacattttggttgtcagagtcaccttctgtccctggaagcatgtcgtggggtgctctaatagtaaatacaaacatggatgagttcagtcctcgtgcacggaggcgaagtgctttgcttacatggcagcacattgcatccttaccacctagcttgccagttgtttattgtggagggttcaacacacaaaaggaatcaactactggtcgttttcttcttgggagatcaagagagcatggtgtggtcggtgatatgagggatacatggcctaatgcccgtgtgaggaaaaatgcttctctaattcgcacttttcatggatttaaaggtaacaaacaaggagctcttgaattcttcaagttggttttcagagccctgtgcctttgctgggatcgccaaacacaggatctacacatagattggattcttttcagaggtagatctcttgttcctgtttcgtgtgaagtggtgagtgataacattgatggacaatatccatcctcccactatccaatagttgctgagtttctgcttcctcgtgctgtgagaattgttgacccacctcctccagaggaaaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

287

Amino Acids

32.5

Weight (kDa)

6.18

Isoelectric Point (pI)

59.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 254 5.2e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 38, 681, 696
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 2 cut(s) 213, 240
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 567, 670, 685
AgsI TTSAA 6 cut(s) 31, 170, 281, 466, 632, 640
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 2 cut(s) 181, 319
AluBI AGCT 3 cut(s) 146, 438, 626
AluI AGCT 3 cut(s) 146, 438, 626
Alw21I GWGCWC 3 cut(s) 343, 385, 628
Alw44I GTGCAC 1 cut(s) 381
AlwI GGATC 3 cut(s) 38, 681, 696
AoxI GGCC 1 cut(s) 551
ApaLI GTGCAC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 300, 545, 763
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 385
BanII GRGCYC 2 cut(s) 628, 658
BauI CACGAG 2 cut(s) 378, 819
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 343, 385, 628
BbvI GCAGC 1 cut(s) 424
BccI CCATC 3 cut(s) 254, 761, 787
BciT130I CCWGG 2 cut(s) 183, 321
BciVI GTATCC 1 cut(s) 538
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 2 cut(s) 108, 435
BfuI GTATCC 1 cut(s) 538
BglII AGATCT 1 cut(s) 722
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
Bme1390I CCNGG 2 cut(s) 183, 321
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 2 cut(s) 183, 321
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 834
BsaJI CCNNGG 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 567, 670, 685
Bse1I ACTGG 2 cut(s) 443, 493
Bse3DI GCAATG 2 cut(s) 238, 417
BseBI CCWGG 2 cut(s) 183, 321
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 3 cut(s) 370, 422, 779
BseLI CCNNNNNNNGG 3 cut(s) 567, 670, 685
BseMI GCAATG 2 cut(s) 238, 417
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 2 cut(s) 443, 493
BseRI GAGGAG 1 cut(s) 837
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 1 cut(s) 424
BseYI CCCAGC 1 cut(s) 669
BshFI GGCC 1 cut(s) 553
BsiHKAI GWGCWC 3 cut(s) 343, 385, 628
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 567, 670, 685
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 1 cut(s) 553
Bsp1286I GDGCHC 4 cut(s) 343, 385, 628, 658
Bsp143I GATC 5 cut(s) 43, 509, 673, 688, 722
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 1 cut(s) 796
BspHI TCATGA 1 cut(s) 37
BspPI GGATC 3 cut(s) 38, 681, 696
BsrDI GCAATG 2 cut(s) 238, 417
BsrI ACTGG 2 cut(s) 443, 493
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 5 cut(s) 43, 509, 673, 688, 722
BssSI CACGAG 2 cut(s) 378, 819
Bst2BI CACGAG 2 cut(s) 378, 819
Bst2UI CCWGG 2 cut(s) 183, 321
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 179, 440
BstDEI CTNAG 1 cut(s) 804
BstF5I GGATG 3 cut(s) 370, 422, 779
BstKTI GATC 5 cut(s) 46, 512, 676, 691, 725
BstMBI GATC 5 cut(s) 43, 509, 673, 688, 722
BstMWI GCNNNNNNNGC 2 cut(s) 409, 675
BstNI CCWGG 2 cut(s) 183, 321
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 181, 319
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 3 cut(s) 43, 688, 722
BstYI RGATCY 3 cut(s) 43, 688, 722
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 1 cut(s) 553
BtsCI GGATG 3 cut(s) 370, 422, 779
Cac8I GCNNGC 2 cut(s) 179, 440
CciI TCATGA 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 8 cut(s) 38, 69, 328, 361, 408, 521, 549, 599
CviJI RGCY 5 cut(s) 146, 438, 553, 626, 656
CviKI_1 RGCY 5 cut(s) 146, 438, 553, 626, 656
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 804
DpnI GATC 5 cut(s) 45, 511, 675, 690, 724
DpnII GATC 5 cut(s) 43, 509, 673, 688, 722
DraI TTTAAA 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 626
Eco24I GRGCYC 2 cut(s) 628, 658
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 626
Eco57I CTGAAG 2 cut(s) 213, 240
EcoICRI GAGCTC 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 181, 319
EcoRV GATATC 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 628, 658
FaeI CATG 8 cut(s) 41, 72, 331, 364, 411, 524, 552, 602
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 302
FatI CATG 8 cut(s) 37, 68, 327, 360, 407, 520, 548, 598
Fnu4HI GCNGC 1 cut(s) 413
FokI GGATG 3 cut(s) 377, 409, 766
FriOI GRGCYC 2 cut(s) 628, 658
Fsp4HI GCNGC 1 cut(s) 413
FspBI CTAG 2 cut(s) 108, 435
GluI GCNGC 1 cut(s) 413
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 834
HaeIII GGCC 1 cut(s) 553
HgaI GACGC 1 cut(s) 88
Hin1II CATG 8 cut(s) 41, 72, 331, 364, 411, 524, 552, 602
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HinfI GANTC 4 cut(s) 124, 305, 479, 706
HphI GGTGA 3 cut(s) 300, 545, 763
Hpy166II GTNNAC 2 cut(s) 383, 838
Hpy188I TCNGA 6 cut(s) 48, 120, 232, 304, 653, 716
Hpy188III TCNNGA 5 cut(s) 38, 207, 513, 629, 851
Hpy8I GTNNAC 2 cut(s) 383, 838
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 259, 275, 321
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 243, 383, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 409, 675
HpyF3I CTNAG 1 cut(s) 804
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 8 cut(s) 41, 72, 331, 364, 411, 524, 552, 602
Kzo9I GATC 5 cut(s) 43, 509, 673, 688, 722
LmnI GCTCC 2 cut(s) 143, 623
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 431
MaeI CTAG 2 cut(s) 108, 435
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 3 cut(s) 103, 306, 611
MalI GATC 5 cut(s) 45, 511, 675, 690, 724
MboI GATC 5 cut(s) 43, 509, 673, 688, 722
MboII GAAGA 8 cut(s) 26, 53, 93, 158, 233, 241, 491, 628
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 3 cut(s) 43, 688, 722
MhlI GDGCHC 4 cut(s) 343, 385, 628, 658
MluCI AATT 7 cut(s) 64, 155, 194, 585, 632, 831, 859
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 71
MseI TTAA 1 cut(s) 606
MspR9I CCNGG 2 cut(s) 183, 321
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 2 cut(s) 183, 321
MwoI GCNNNNNNNGC 2 cut(s) 409, 675
NdeII GATC 5 cut(s) 43, 509, 673, 688, 722
NlaIII CATG 8 cut(s) 41, 72, 331, 364, 411, 524, 552, 602
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 331
PagI TCATGA 1 cut(s) 37
PfeI GAWTC 3 cut(s) 124, 479, 706
PkrI GCNGC 1 cut(s) 414
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 628
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 181, 319
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 181, 319
PspPI GGNCC 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 215
PsuI RGATCY 3 cut(s) 43, 688, 722
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SacI GAGCTC 1 cut(s) 628
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 1 cut(s) 413
Sau3AI GATC 5 cut(s) 43, 509, 673, 688, 722
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 183, 321
SduI GDGCHC 4 cut(s) 343, 385, 628, 658
SfaNI GCATC 1 cut(s) 431
SinI GGWCC 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 7 cut(s) 64, 155, 194, 585, 632, 831, 859
SspMI CTAG 2 cut(s) 108, 435
SstI GAGCTC 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 181, 319
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 29
TasI AATT 7 cut(s) 64, 155, 194, 585, 632, 831, 859
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 3 cut(s) 124, 479, 706
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 4 cut(s) 26, 54, 57, 587
TspGWI ACGGA 1 cut(s) 400
VneI GTGCAC 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 632
XceI RCATGY 1 cut(s) 331
XspI CTAG 2 cut(s) 108, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.