Rmu_sc0006329.1_g000001

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006329.1
OG0011554
Physical Location & Seq
Forward (+)
1 .. 3937
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
gatgggcatggtccttgtggtcatatggtggcgaagaaggtgagggattctctacctttgaaggtcagctcacaatgggtgttgaacaccagcgccgcggatactaaccacgccggtgcgtctgtggagaacaaggacagccatggtgaatttttcgccacattgaaagattcatttttgaaggcttttaaggtcatggacaaggagcttaagttgcatcctcgtattgattgctattgcagtgggacaactgcagttacgttggtcaagcagggtcaggatcttgttattgcaaatattggggactctagagctgtgctaggcacaagagatgaagggggttctcttgttgcagttcagttgaccgtagacttaaaaccaaatcttccaagggaagccgagaggattacgttatgtaaaggtcgagtatttgctctccacaacgagcctgatgtcactcgagtttggttgcccaacagtaattcacctgggcttgctatggcccgggcttttggagatttctgcctaaaggattatggtttaatctctgtccctgatgtttcttatcgtcgcctcactgagaaggatgaatttgtagtactggctacagatgggatctgggatgttctctctaacaaagaagttgtggatatagtggcagcaaccccacgaccctctgctgctcgagccctggtggagtcagccaatcgagcttggagatttaaacacccatatgccaaggttgatgattgtgccgttgtttgcctcttcctcacttccggtgcttcagacaatacttccctttcctcaccagaggagaagaaagtgaactcaatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

279

Amino Acids

30.34

Weight (kDa)

6.72

Isoelectric Point (pI)

21.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 41 - 222 7.7e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 96, 98
AclWI GGATC 2 cut(s) 288, 623
AcsI RAATTY 2 cut(s) 149, 590
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 1 cut(s) 600
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 61, 85, 166, 181
AjnI CCWGG 2 cut(s) 487, 690
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 4 cut(s) 69, 208, 314, 713
AluI AGCT 4 cut(s) 69, 208, 314, 713
AlwI GGATC 2 cut(s) 288, 623
Ama87I CYCGRG 3 cut(s) 459, 504, 684
AoxI GGCC 1 cut(s) 501
ApeKI GCWGC 2 cut(s) 659, 680
ApoI RAATTY 2 cut(s) 149, 590
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 11, 502
AsuC2I CCSGG 2 cut(s) 505, 506
AsuHPI GGTGA 4 cut(s) 52, 158, 477, 801
AvaI CYCGRG 3 cut(s) 459, 504, 684
AvaII GGWCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 691
BbvI GCAGC 2 cut(s) 667, 671
BccI CCATC 1 cut(s) 605
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 2 cut(s) 489, 692
BciVI GTATCC 1 cut(s) 94
BcnI CCSGG 2 cut(s) 505, 506
BfaI CTAG 2 cut(s) 309, 320
BfmI CTRYAG 2 cut(s) 252, 606
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 209
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 3 cut(s) 96, 660, 681
BlsI GCNGC 3 cut(s) 97, 661, 682
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 4 cut(s) 489, 505, 506, 692
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 3 cut(s) 459, 504, 684
BmgT120I GGNCC 2 cut(s) 11, 502
BmrFI CCNGG 4 cut(s) 489, 505, 506, 692
BmsI GCATC 1 cut(s) 226
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpuMI CCSGG 2 cut(s) 505, 506
BsaJI CCNNGG 7 cut(s) 96, 142, 389, 488, 504, 690, 738
BsaWI WCCGGW 1 cut(s) 779
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 606
BseBI CCWGG 2 cut(s) 489, 692
BseDI CCNNGG 7 cut(s) 96, 142, 389, 488, 504, 690, 738
BseGI GGATG 3 cut(s) 217, 592, 628
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 1 cut(s) 830
BseXI GCAGC 2 cut(s) 667, 671
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 1 cut(s) 503
BsiHKCI CYCGRG 3 cut(s) 459, 504, 684
BsiSI CCGG 3 cut(s) 114, 505, 780
BslFI GGGAC 3 cut(s) 259, 317, 536
BsmFI GGGAC 3 cut(s) 259, 317, 536
BsnI GGCC 1 cut(s) 503
BsoBI CYCGRG 3 cut(s) 459, 504, 684
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 2 cut(s) 280, 615
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 96, 98
BspANI GGCC 1 cut(s) 503
BspCNI CTCAG 1 cut(s) 571
BspFNI CGCG 1 cut(s) 98
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 2 cut(s) 288, 623
BspTI CTTAAG 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 7 cut(s) 96, 142, 389, 488, 504, 690, 738
BssMI GATC 2 cut(s) 280, 615
BssT1I CCWWGG 3 cut(s) 142, 389, 738
Bst2UI CCWGG 2 cut(s) 489, 692
Bst4CI ACNGT 2 cut(s) 367, 479
Bst6I CTCTTC 1 cut(s) 773
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 2 cut(s) 96, 142
BstF5I GGATG 3 cut(s) 217, 592, 628
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 2 cut(s) 283, 618
BstMBI GATC 2 cut(s) 280, 615
BstMWI GCNNNNNNNGC 3 cut(s) 214, 686, 710
BstNI CCWGG 2 cut(s) 489, 692
BstSCI CCNGG 4 cut(s) 487, 503, 504, 690
BstSFI CTRYAG 2 cut(s) 252, 606
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 667, 671
BstX2I RGATCY 2 cut(s) 280, 615
BstYI RGATCY 2 cut(s) 280, 615
BsuI GTATCC 1 cut(s) 94
BsuRI GGCC 1 cut(s) 503
BtgI CCRYGG 2 cut(s) 96, 142
BtsCI GGATG 3 cut(s) 217, 592, 628
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 247, 576
Cac8I GCNNGC 1 cut(s) 495
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 11, 502
Cfr42I CCGCGG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 504
CseI GACGC 1 cut(s) 108
Csp6I GTAC 1 cut(s) 599
CviAII CATG 3 cut(s) 8, 143, 196
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 1 cut(s) 579
DpnI GATC 2 cut(s) 282, 617
DpnII GATC 2 cut(s) 280, 615
DraI TTTAAA 1 cut(s) 724
Eam1104I CTCTTC 1 cut(s) 773
EarI CTCTTC 1 cut(s) 773
Eco130I CCWWGG 3 cut(s) 142, 389, 738
Eco24I GRGCYC 1 cut(s) 691
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 3 cut(s) 459, 504, 684
EcoRII CCWGG 2 cut(s) 487, 690
EcoT14I CCWWGG 3 cut(s) 142, 389, 738
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 3 cut(s) 142, 389, 738
FaeI CATG 3 cut(s) 11, 146, 199
FaqI GGGAC 3 cut(s) 259, 317, 536
FatI CATG 3 cut(s) 7, 142, 195
FauNDI CATATG 2 cut(s) 24, 733
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 96, 660, 681
FokI GGATG 3 cut(s) 204, 599, 635
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 3 cut(s) 96, 660, 681
FspBI CTAG 2 cut(s) 309, 320
GlaI GCGC 1 cut(s) 94
GluI GCNGC 3 cut(s) 96, 660, 681
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 503
HapII CCGG 3 cut(s) 114, 505, 780
HgaI GACGC 1 cut(s) 108
HhaI GCGC 1 cut(s) 95
Hin1II CATG 3 cut(s) 11, 146, 199
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 4 cut(s) 47, 170, 305, 698
HpaII CCGG 3 cut(s) 114, 505, 780
HphI GGTGA 4 cut(s) 52, 158, 477, 801
Hpy166II GTNNAC 3 cut(s) 363, 370, 829
Hpy188I TCNGA 1 cut(s) 790
Hpy188III TCNNGA 2 cut(s) 278, 309
Hpy8I GTNNAC 3 cut(s) 363, 370, 829
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 5 cut(s) 31, 55, 175, 329, 577
HpyCH4III ACNGT 2 cut(s) 367, 479
HpyCH4IV ACGT 2 cut(s) 260, 410
HpyCH4V TGCA 5 cut(s) 217, 240, 254, 293, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 686, 710
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 2 cut(s) 260, 410
Hsp92II CATG 3 cut(s) 11, 146, 199
HspAI GCGC 1 cut(s) 93
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 2 cut(s) 280, 615
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 2 cut(s) 667, 671
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 309, 320
MaeII ACGT 2 cut(s) 260, 410
MaeIII GTNAC 2 cut(s) 256, 454
MalI GATC 2 cut(s) 282, 617
MboI GATC 2 cut(s) 280, 615
MboII GAAGA 4 cut(s) 46, 377, 760, 832
MflI RGATCY 2 cut(s) 280, 615
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 3 cut(s) 149, 481, 590
MlyI GAGTC 2 cut(s) 299, 707
MnlI CCTC 9 cut(s) 36, 231, 396, 584, 685, 776, 782, 808, 817
MseI TTAA 5 cut(s) 189, 210, 374, 542, 723
MslI CAYNNNNRTG 2 cut(s) 114, 732
MspA1I CMGCKG 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 3 cut(s) 114, 505, 780
MspR9I CCNGG 4 cut(s) 489, 505, 506, 692
MvaI CCWGG 2 cut(s) 489, 692
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 214, 686, 710
NciI CCSGG 2 cut(s) 505, 506
NcoI CCATGG 1 cut(s) 142
NdeI CATATG 2 cut(s) 24, 733
NdeII GATC 2 cut(s) 280, 615
NlaIII CATG 3 cut(s) 11, 146, 199
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 454
OliI CACNNNNGTG 1 cut(s) 114
PaeR7I CTCGAG 2 cut(s) 459, 684
PfeI GAWTC 2 cut(s) 47, 170
PkrI GCNGC 3 cut(s) 97, 661, 682
PleI GAGTC 2 cut(s) 299, 706
PpsI GAGTC 2 cut(s) 299, 706
Psp6I CCWGG 2 cut(s) 487, 690
PspGI CCWGG 2 cut(s) 487, 690
PspPI GGNCC 2 cut(s) 11, 502
PspXI VCTCGAGB 2 cut(s) 459, 684
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 2 cut(s) 280, 615
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 2 cut(s) 114, 732
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 5 cut(s) 189, 210, 374, 542, 723
SatI GCNGC 3 cut(s) 96, 660, 681
Sau3AI GATC 2 cut(s) 280, 615
Sau96I GGNCC 2 cut(s) 11, 502
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 2 cut(s) 299, 707
ScrFI CCNGG 4 cut(s) 489, 505, 506, 692
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 2 cut(s) 252, 606
Sfr274I CTCGAG 2 cut(s) 459, 684
Sfr303I CCGCGG 1 cut(s) 99
SgrAI CRCCGGYG 1 cut(s) 113
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 1 cut(s) 11
SlaI CTCGAG 2 cut(s) 459, 684
SmaI CCCGGG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 114, 732
SmlI CTYRAG 3 cut(s) 209, 459, 684
SmoI CTYRAG 3 cut(s) 209, 459, 684
SrfI GCCCGGGC 1 cut(s) 506
Sse9I AATT 3 cut(s) 149, 481, 590
SsiI CCGC 2 cut(s) 96, 98
SspI AATATT 1 cut(s) 298
SspMI CTAG 2 cut(s) 309, 320
StyD4I CCNGG 4 cut(s) 487, 503, 504, 690
StyI CCWWGG 3 cut(s) 142, 389, 738
TaaI ACNGT 2 cut(s) 367, 479
TaiI ACGT 2 cut(s) 263, 413
TaqI TCGA 4 cut(s) 424, 460, 685, 709
TasI AATT 3 cut(s) 149, 481, 590
TatI WGTACW 1 cut(s) 598
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 2 cut(s) 47, 170
Tru1I TTAA 5 cut(s) 189, 210, 374, 542, 723
Tru9I TTAA 5 cut(s) 189, 210, 374, 542, 723
TscAI CASTG 2 cut(s) 247, 583
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 659, 680
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 3 cut(s) 162, 348, 603
TspMI CCCGGG 1 cut(s) 504
TspRI CASTG 2 cut(s) 247, 583
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 2 cut(s) 149, 590
XbaI TCTAGA 1 cut(s) 308
XhoI CTCGAG 2 cut(s) 459, 684
XmaI CCCGGG 1 cut(s) 504
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 2 cut(s) 309, 320
ZrmI AGTACT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.