Rmu_sc0006449.1_g000007

phosphate transporter PHO1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006449.1
OG0011751
Physical Location & Seq
Forward (+)
23016 .. 26161
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1233 bp
atgagcataaattggctgggaagtcatagcatctccggcagcggtttagtaaactttccggctaaaaggtggccactatacctctccaagtggaggaataggatggttcacaagaagcttgcctcatcagcaagcaagggagacatgtacgaaactgaattgttcgagcaacttgctgatactgatgctgcgaaagagttttttgcatgcttggaccttcaactcaacaaggtgaatcagttctaccagaaaaaggagaaagagttcatggagagagggcagtccttgaggaagcagatggatatactcattgagctcaaaaccgctttcaagcaacagcgcgccaaaggtggagcatctgctcaggactccaaggaagaagcctctatctcatgcaccttctcatctgaggaggactctgtgaagggcaaaacggaacttgaacagccgcaagaaacagatgagttggagaaaaatgatgaagtagcctacacagaagtcccaatatcaggcgaattggtgaaaccaataagaatgaaaagtgaggatattgggaaacaactgaggagcgcctcaagccgttctttcagttgccaagggaagaacttaaagataaacattcctctgactacaccgtcacgcaccttctcagcaatcagttatttagtttgggaagatctagttaatcagtcctcaaagaaatgtacttcagaaggcatgagtaagctgcacatcaacaaaaccaagttgcatcatgcagataagatgatcagaggagcttttgttgagctgtacaaaggcttgggatatctcaaaacctacaggaacttgaacatgcttgcttttataaagattttaaagaagtttgacaaggcgactgaaaaacaagtgcttccaatctatctaaaagttgttgagagttcctatttcaacagctcagataaggttatgaatctagcagatgaagttgaggagctgtttaccaaacacttcgctgaagaagaccgaagaaaggccatgaaataccttaaaccacatcatcgcaaagaatcacactctgtaacgttcttcattggtctattcactgggtgtttcattgcactctttgctggatatgtcattatggctcatattactgggttgtacagaaggcaaccaaattctatatacatggaaactgcctaccctatactcaggcaagtatttctgcaattaaattctttggcatcttaa

Protein Analysis

410

Amino Acids

46.99

Weight (kDa)

9.2

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 37 - 305 1.4e-48 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011751)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68740 AT1G68740
fragaria_vesca FvH4_4g28410
malus_domestica MD13G1049800.v1.1 MD16G1050800.v1.1
prunus_persica Prupe.1G301900_v2.0.a1
pyrus_communis pycom13g04410 pycom13g04420 pycom16g04450
rosa_chinensis RchiOBHm_Chr4g0436361
rosa_laevigata RLG00000006525
rosa_multiflora Rmu_sc0006449.1_g000007
rosa_roxburghii Rroxscaffold_5G00377530
rosa_rugosa Rorug04G0293300
rosa_samantha Rh4AG347500 Rh4BG356300 Rh4CG370900 Rh4DG350000
rosa_wichuraiana Rw4G030380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
AasI GACNNNNNNGTC 1 cut(s) 634
AccII CGCG 1 cut(s) 342
AciI CCGC 3 cut(s) 42, 324, 449
AclI AACGTT 1 cut(s) 1064
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 2 cut(s) 1159, 1216
AcuI CTGAAG 2 cut(s) 693, 1017
AdeI CACNNNGTG 1 cut(s) 1089
AfaI GTAC 4 cut(s) 149, 706, 794, 1145
AfiI CCNNNNNNNGG 4 cut(s) 93, 253, 509, 1012
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 5 cut(s) 221, 331, 443, 832, 931
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 7 cut(s) 118, 316, 727, 779, 790, 936, 976
AluI AGCT 7 cut(s) 118, 316, 727, 779, 790, 936, 976
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 2 cut(s) 71, 1014
ApeKI GCWGC 3 cut(s) 39, 188, 727
ApoI RAATTY 2 cut(s) 1159, 1216
Asp700I GAANNNNTTC 2 cut(s) 239, 263
AspLEI GCGC 3 cut(s) 342, 344, 572
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 244, 532
AvaII GGWCC 1 cut(s) 214
BalI TGGCCA 1 cut(s) 73
BanII GRGCYC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 1008
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 3 cut(s) 51, 175, 714
BccI CCATC 2 cut(s) 97, 292
BceAI ACGGC 1 cut(s) 564
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BclI TGATCA 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 2 cut(s) 680, 956
BfmI CTRYAG 1 cut(s) 820
BfoI RGCGCY 1 cut(s) 573
BglII AGATCT 1 cut(s) 676
BisI GCNGC 4 cut(s) 40, 189, 449, 728
BlsI GCNGC 4 cut(s) 41, 190, 450, 729
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmrI ACTGGG 2 cut(s) 1095, 1146
BmsI GCATC 4 cut(s) 39, 175, 365, 760
BmuI ACTGGG 2 cut(s) 1095, 1146
BpiI GAAGAC 1 cut(s) 1008
BplI GAGNNNNNCTC 2 cut(s) 401, 433
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 307, 559
BsaJI CCNNGG 2 cut(s) 372, 595
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 768, 798
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 253, 509, 1012
Bse1I ACTGG 2 cut(s) 1090, 1141
Bse3DI GCAATG 1 cut(s) 1095
BseDI CCNNGG 2 cut(s) 372, 595
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 4 cut(s) 93, 253, 509, 1012
BseMI GCAATG 1 cut(s) 1095
BseMII CTCAG 6 cut(s) 377, 399, 554, 663, 951, 1207
BseNI ACTGG 2 cut(s) 1090, 1141
BsePI GCGCGC 1 cut(s) 340
BseRI GAGGAG 4 cut(s) 425, 580, 789, 986
BseXI GCAGC 3 cut(s) 51, 175, 714
BseYI CCCAGC 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 713
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 2 cut(s) 73, 1016
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 2 cut(s) 36, 59
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 4 cut(s) 93, 253, 509, 1012
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 2 cut(s) 73, 1016
Bsp1286I GDGCHC 1 cut(s) 318
Bsp1407I TGTACA 2 cut(s) 792, 1143
Bsp143I GATC 2 cut(s) 676, 768
BspACI CCGC 3 cut(s) 42, 324, 449
BspANI GGCC 2 cut(s) 73, 1016
BspCNI CTCAG 6 cut(s) 376, 400, 555, 662, 950, 1206
BspFNI CGCG 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 1095
BsrGI TGTACA 2 cut(s) 792, 1143
BsrI ACTGG 2 cut(s) 1090, 1141
BssECI CCNNGG 2 cut(s) 372, 595
BssHII GCGCGC 1 cut(s) 340
BssMI GATC 2 cut(s) 676, 768
BssT1I CCWWGG 2 cut(s) 372, 595
Bst4CI ACNGT 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstAUI TGTACA 2 cut(s) 792, 1143
BstC8I GCNNGC 5 cut(s) 120, 133, 208, 342, 840
BstDEI CTNAG 6 cut(s) 363, 408, 563, 649, 937, 1193
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 1 cut(s) 573
BstHHI GCGC 3 cut(s) 342, 344, 572
BstKTI GATC 2 cut(s) 679, 771
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 676, 768
BstMWI GCNNNNNNNGC 4 cut(s) 36, 128, 576, 1106
BstNSI RCATGY 3 cut(s) 148, 210, 838
BstSFI CTRYAG 1 cut(s) 820
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 3 cut(s) 51, 175, 714
BstV2I GAAGAC 1 cut(s) 1008
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BsuRI GGCC 2 cut(s) 73, 1016
BtgZI GCGATG 1 cut(s) 1025
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 1083
Cac8I GCNNGC 5 cut(s) 120, 133, 208, 342, 840
CfoI GCGC 3 cut(s) 342, 344, 572
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 4 cut(s) 148, 705, 793, 1144
CviAII CATG 9 cut(s) 145, 207, 268, 393, 718, 755, 835, 1018, 1171
CviQI GTAC 4 cut(s) 148, 705, 793, 1144
DdeI CTNAG 6 cut(s) 363, 408, 563, 649, 937, 1193
DpnI GATC 2 cut(s) 678, 770
DpnII GATC 2 cut(s) 676, 768
DraI TTTAAA 1 cut(s) 858
DraIII CACNNNGTG 1 cut(s) 1089
DrdI GACNNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 634
EaeI YGGCCR 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 372, 595
Eco24I GRGCYC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 809
Eco47I GGWCC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 693, 1017
EcoICRI GAGCTC 1 cut(s) 316
EcoRV GATATC 1 cut(s) 809
EcoT14I CCWWGG 2 cut(s) 372, 595
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 372, 595
FaeI CATG 9 cut(s) 148, 210, 271, 396, 721, 758, 838, 1021, 1174
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FaqI GGGAC 1 cut(s) 485
FatI CATG 9 cut(s) 144, 206, 267, 392, 717, 754, 834, 1017, 1170
FbaI TGATCA 1 cut(s) 768
Fnu4HI GCNGC 4 cut(s) 40, 189, 449, 728
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 4 cut(s) 40, 189, 449, 728
FspBI CTAG 2 cut(s) 680, 956
GlaI GCGC 3 cut(s) 341, 343, 571
GluI GCNGC 4 cut(s) 40, 189, 449, 728
GsaI CCCAGC 1 cut(s) 20
HaeII RGCGCY 1 cut(s) 573
HaeIII GGCC 2 cut(s) 73, 1016
HapII CCGG 2 cut(s) 36, 59
HhaI GCGC 3 cut(s) 342, 344, 572
Hin1II CATG 9 cut(s) 148, 210, 271, 396, 721, 758, 838, 1021, 1174
Hin6I GCGC 3 cut(s) 340, 342, 570
HinP1I GCGC 3 cut(s) 340, 342, 570
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 5 cut(s) 235, 368, 416, 952, 1049
HpaII CCGG 2 cut(s) 36, 59
HphI GGTGA 2 cut(s) 244, 532
Hpy166II GTNNAC 3 cut(s) 52, 109, 981
Hpy188I TCNGA 5 cut(s) 409, 627, 712, 773, 940
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 3 cut(s) 52, 109, 981
HpyAV CCTTC 6 cut(s) 227, 409, 418, 655, 707, 1143
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 7 cut(s) 206, 396, 730, 751, 758, 1100, 1210
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 128, 576, 1106
HpyF3I CTNAG 6 cut(s) 363, 408, 563, 649, 937, 1193
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 9 cut(s) 148, 210, 271, 396, 721, 758, 838, 1021, 1174
HspAI GCGC 3 cut(s) 340, 342, 570
Ksp22I TGATCA 1 cut(s) 768
Kzo9I GATC 2 cut(s) 676, 768
LmnI GCTCC 4 cut(s) 353, 567, 776, 973
Lsp1109I GCAGC 3 cut(s) 51, 175, 714
LweI GCATC 4 cut(s) 39, 175, 365, 760
MaeI CTAG 2 cut(s) 680, 956
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 2 cut(s) 636, 1060
MalI GATC 2 cut(s) 678, 770
MboI GATC 2 cut(s) 676, 768
MboII GAAGA 7 cut(s) 389, 613, 686, 1010, 1013, 1020, 1060
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 1 cut(s) 318
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 6 cut(s) 10, 158, 515, 1159, 1211, 1216
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 2 cut(s) 362, 410
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 1 cut(s) 73
MroXI GAANNNNTTC 2 cut(s) 239, 263
MscI TGGCCA 1 cut(s) 73
MseI TTAA 6 cut(s) 608, 684, 857, 1029, 1214, 1231
Msp20I TGGCCA 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 2 cut(s) 36, 59
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 36, 128, 576, 1106
NdeII GATC 2 cut(s) 676, 768
NlaIII CATG 9 cut(s) 148, 210, 271, 396, 721, 758, 838, 1021, 1174
NmuCI GTSAC 1 cut(s) 636
NspI RCATGY 3 cut(s) 148, 210, 838
PaeI GCATGC 1 cut(s) 210
PauI GCGCGC 1 cut(s) 340
PciI ACATGT 1 cut(s) 144
PdmI GAANNNNTTC 2 cut(s) 239, 263
PfeI GAWTC 3 cut(s) 235, 952, 1049
PkrI GCNGC 4 cut(s) 41, 190, 450, 729
PleI GAGTC 2 cut(s) 362, 410
PpsI GAGTC 2 cut(s) 362, 410
PscI ACATGT 1 cut(s) 144
PsiI TTATAA 1 cut(s) 848
Psp124BI GAGCTC 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 1064
PspFI CCCAGC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 214
PsuI RGATCY 1 cut(s) 676
PteI GCGCGC 1 cut(s) 340
RsaI GTAC 4 cut(s) 149, 706, 794, 1145
RsaNI GTAC 4 cut(s) 148, 705, 793, 1144
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 6 cut(s) 608, 684, 857, 1029, 1214, 1231
SatI GCNGC 4 cut(s) 40, 189, 449, 728
Sau3AI GATC 2 cut(s) 676, 768
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 2 cut(s) 362, 410
SduI GDGCHC 1 cut(s) 318
SfaNI GCATC 4 cut(s) 39, 175, 365, 760
SfcI CTRYAG 1 cut(s) 820
SinI GGWCC 1 cut(s) 214
SmlI CTYRAG 2 cut(s) 286, 574
SmoI CTYRAG 2 cut(s) 286, 574
SphI GCATGC 1 cut(s) 210
Sse9I AATT 6 cut(s) 10, 158, 515, 1159, 1211, 1216
SsiI CCGC 3 cut(s) 42, 324, 449
SspMI CTAG 2 cut(s) 680, 956
SstI GAGCTC 1 cut(s) 318
StyI CCWWGG 2 cut(s) 372, 595
TaaI ACNGT 1 cut(s) 636
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 1 cut(s) 165
TaqII GACCGA 1 cut(s) 1020
TasI AATT 6 cut(s) 10, 158, 515, 1159, 1211, 1216
TatI WGTACW 3 cut(s) 704, 792, 1143
TauI GCSGC 1 cut(s) 451
TfiI GAWTC 3 cut(s) 235, 952, 1049
Tru1I TTAA 6 cut(s) 608, 684, 857, 1029, 1214, 1231
Tru9I TTAA 6 cut(s) 608, 684, 857, 1029, 1214, 1231
TscAI CASTG 1 cut(s) 1090
TseFI GTSAC 1 cut(s) 636
TseI GCWGC 3 cut(s) 39, 188, 727
Tsp45I GTSAC 1 cut(s) 636
TspDTI ATGAA 8 cut(s) 256, 495, 551, 965, 978, 1034, 1060, 1084
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 1090
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 2 cut(s) 1159, 1216
XceI RCATGY 3 cut(s) 148, 210, 838
XmnI GAANNNNTTC 2 cut(s) 239, 263
XspI CTAG 2 cut(s) 680, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.