Rmu_sc0007286.1_g000001

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007286.1
OG0014846
Physical Location & Seq
Reverse (-)
1 .. 2503
2503 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 412 bp
atgcccctacatgtatactggcggttttggtatacggtgttggaaggggttagtctcttcttgaggttggctgtaaggaggtatcgggactccttggataggttgattctttgtagcctgaagagtgggcgattcatgtggttagagtttagtctctatcggctcatggtgtctgtaactgttggtcttacattagggaaagcactaaagtccatggatccttcagtcatgcaaaaccctgagctggccagcatcataaatcaagagcagcaaagggcgatggtcaatgagatggtgggaaagcttacgagtgcatgctgggacaagtgcatcacctctgcacctgggagcaagttcagttccagtgaaacagcttgcctctcaaattgtgcccggcgctatttggatatga

Protein Analysis

137

Amino Acids

15.72

Weight (kDa)

9.27

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Tim10_DDP PF02953 85 - 137 1.4e-20 Tim10/DDP family zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 15, 32
AciI CCGC 1 cut(s) 22
AclWI GGATC 2 cut(s) 212, 225
AcoI YGGCCR 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 140, 207
AfiI CCNNNNNNNGG 2 cut(s) 99, 244
AflIII ACRYGT 1 cut(s) 10
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 3 cut(s) 244, 304, 374
AluI AGCT 3 cut(s) 244, 304, 374
Alw26I GTCTC 2 cut(s) 59, 158
AlwI GGATC 2 cut(s) 212, 225
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 268
AspLEI GCGC 1 cut(s) 399
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 248
BamHI GGATCC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 280
BccI CCATC 2 cut(s) 274, 286
BciT130I CCWGG 1 cut(s) 345
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 59, 158
BfoI RGCGCY 1 cut(s) 400
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 2 cut(s) 345, 394
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 345, 394
BmsI GCATC 2 cut(s) 261, 339
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 394
BsaJI CCNNGG 3 cut(s) 93, 213, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 244
Bse1I ACTGG 2 cut(s) 23, 363
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 93, 213, 344
BseLI CCNNNNNNNGG 2 cut(s) 99, 244
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 23, 363
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 1 cut(s) 280
BseYI CCCAGC 1 cut(s) 318
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 1 cut(s) 248
BsiSI CCGG 1 cut(s) 394
BslFI GGGAC 2 cut(s) 101, 335
BslI CCNNNNNNNGG 2 cut(s) 99, 244
BsmAI GTCTC 2 cut(s) 59, 158
BsmFI GGGAC 2 cut(s) 101, 335
BsnI GGCC 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 1 cut(s) 217
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 2 cut(s) 212, 225
BsrI ACTGG 2 cut(s) 23, 363
BssECI CCNNGG 3 cut(s) 93, 213, 344
BssMI GATC 1 cut(s) 217
BssNAI GTATAC 2 cut(s) 16, 33
BssT1I CCWWGG 2 cut(s) 93, 213
Bst1107I GTATAC 2 cut(s) 16, 33
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 37, 181
Bst6I CTCTTC 2 cut(s) 62, 116
BstC8I GCNNGC 4 cut(s) 246, 250, 316, 376
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 213
BstENI CCTNNNNNAGG 1 cut(s) 97
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 1 cut(s) 220
BstMAI GTCTC 2 cut(s) 59, 158
BstMBI GATC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 2 cut(s) 14, 318
BstSCI CCNGG 2 cut(s) 343, 392
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BstZ17I GTATAC 2 cut(s) 16, 33
BsuRI GGCC 1 cut(s) 248
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 4 cut(s) 246, 250, 316, 376
CfoI GCGC 1 cut(s) 399
CviAII CATG 6 cut(s) 11, 136, 166, 214, 229, 315
CviJI RGCY 7 cut(s) 71, 117, 163, 244, 248, 304, 374
CviKI_1 RGCY 7 cut(s) 71, 117, 163, 244, 248, 304, 374
DdeI CTNAG 1 cut(s) 240
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 62, 116
EarI CTCTTC 2 cut(s) 62, 116
Eco130I CCWWGG 2 cut(s) 93, 213
Eco57I CTGAAG 2 cut(s) 140, 207
EcoNI CCTNNNNNAGG 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 93, 213
ErhI CCWWGG 2 cut(s) 93, 213
FaeI CATG 6 cut(s) 14, 139, 169, 217, 232, 318
FaqI GGGAC 2 cut(s) 101, 335
FatI CATG 6 cut(s) 10, 135, 165, 213, 228, 314
FblI GTMKAC 2 cut(s) 15, 32
Fnu4HI GCNGC 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 269
GlaI GCGC 1 cut(s) 398
GluI GCNGC 1 cut(s) 269
GsaI CCCAGC 1 cut(s) 322
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 1 cut(s) 248
HapII CCGG 1 cut(s) 394
HhaI GCGC 1 cut(s) 399
Hin1II CATG 6 cut(s) 14, 139, 169, 217, 232, 318
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 3 cut(s) 89, 106, 132
HpaII CCGG 1 cut(s) 394
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 16, 33
Hpy188III TCNNGA 3 cut(s) 61, 86, 263
Hpy8I GTNNAC 2 cut(s) 16, 33
HpyAV CCTTC 2 cut(s) 38, 231
HpyCH4III ACNGT 2 cut(s) 37, 181
HpyCH4V TGCA 4 cut(s) 232, 314, 330, 341
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 6 cut(s) 14, 139, 169, 217, 232, 318
HspAI GCGC 1 cut(s) 397
Kzo9I GATC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 2 cut(s) 261, 339
MaeIII GTNAC 1 cut(s) 175
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MboII GAAGA 2 cut(s) 49, 133
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 1 cut(s) 385
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 21
MnlI CCTC 4 cut(s) 57, 72, 346, 389
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
Msp20I TGGCCA 1 cut(s) 248
MspI CCGG 1 cut(s) 394
MspR9I CCNGG 2 cut(s) 345, 394
MvaI CCWGG 1 cut(s) 345
NciI CCSGG 1 cut(s) 394
NcoI CCATGG 1 cut(s) 213
NdeII GATC 1 cut(s) 217
NlaIII CATG 6 cut(s) 14, 139, 169, 217, 232, 318
NlaIV GGNNCC 1 cut(s) 219
NspI RCATGY 2 cut(s) 14, 318
PaeI GCATGC 1 cut(s) 318
PciI ACATGT 1 cut(s) 10
PfeI GAWTC 2 cut(s) 106, 132
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PscI ACATGT 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 219
PsuI RGATCY 1 cut(s) 217
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 1 cut(s) 217
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 345, 394
SduI GDGCHC 1 cut(s) 394
SetI ASST 8 cut(s) 68, 83, 104, 246, 306, 338, 346, 376
SfaNI GCATC 2 cut(s) 261, 339
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SphI GCATGC 1 cut(s) 318
Sse9I AATT 1 cut(s) 385
SsiI CCGC 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 343, 392
StyI CCWWGG 2 cut(s) 93, 213
TaaI ACNGT 2 cut(s) 37, 181
TasI AATT 1 cut(s) 385
TfiI GAWTC 2 cut(s) 106, 132
TscAI CASTG 1 cut(s) 370
TseI GCWGC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 124
TspRI CASTG 1 cut(s) 370
XagI CCTNNNNNAGG 1 cut(s) 97
XceI RCATGY 2 cut(s) 14, 318
XmiI GTMKAC 2 cut(s) 15, 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.