Rh5AG051900

quinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014846
Physical Location & Seq
Reverse (-)
4378000 .. 4380997
2998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG051900.1

Sequence Viewer

Length: 1005 bp
ATGCTTCGTCTCTCTCCTCTGAAGGCGGTGTGGGCTGTTCTGATGGCTGGATTGAGCGGGGCTTCTCTGAACTGGGGACCCAGGTTTGCAGTTTGGAGATGGGGTTGCGATTGGGTTGAGCTTGACATTCGTTGTAGCGGTATTACATGGTGGAACCTCCGGGATCAGCAAGAAGATGGGGCAATACATCAGGGCAGATGGTGGTTGACGGTTTCGAGCCTATGGAGGCTATGCAGCGGTGTTTTTGGAAGATGGCGATGGCTGGAGATCTTGTGGGTTCGAGATTTGAAACTGGGTGATGGGGTTGAATTGATGTTTTGGGTCTTGGAATGGGTGATCATCGGTGGTTGGGTTGGCATCAAGTTGCTATGTCGAAGAGGGCGGTGGTCGTGTTTCGTTAACCCCCAATGCTCTTGCAGCGGGATGACGGTGGCAATCGGGCAGATGGAGGCGGTGGTCTTGGGGAGGATAGTTGTTCGTGGTAGGCCACAGTGGTTACCTCACCAGGCGGACCAAGTACGGGAAGTGGAAGACCCCAAAATAAAAGAAGACGAGGTCCTAGTCAAAGTCGAGGCCACGTCACTGAACCAGGCCGACAGCTTACAGCGGCTTGGCTTCTACCCACCACCTCCGGGTTCCAGCGAGTTACTGGGCCTGGAATGTTCTGGAACCATCGGGGCCGTAGGGGAACAAGTGTCACGCTGGAAAGCCGGCGATCAGGTGTGTGCTGTTCTTACGGGAGGAGGCTATGCCGAGAAGGTGGCTGTTCCGGCTGGACAAATTCTTCCACTCCCAACCGGTATTTCTTTGAAGGATGCAGCTAGTCTCCCAGAGGTGGCATGCACTGTCTGGTCGACTGTTTTCACGACGAGTCGACTATCTTCTGGGGAAATATTTTTGCGGCTGGCTTGCTATCTAGAAGTCGAGAAAAACAAAGCATTCATAGTGAGTGAGACAGAGAAAAATGTATGGTCTGCAATTGCGGCCGGCAAGGTGAAAAAGTGA

Protein Analysis

334

Amino Acids

37.09

Weight (kDa)

8.27

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 183 - 265 8.5e-15 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 2 cut(s) 854, 874
AclWI GGATC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 984
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 1 cut(s) 519
AfiI CCNNNNNNNGG 3 cut(s) 520, 632, 835
AgeI ACCGGT 1 cut(s) 797
AgsI TTSAA 3 cut(s) 289, 308, 811
AjiI CACGTC 1 cut(s) 579
AjnI CCWGG 4 cut(s) 80, 504, 588, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 787, 819
AluBI AGCT 3 cut(s) 121, 600, 821
AluI AGCT 3 cut(s) 121, 600, 821
Alw26I GTCTC 3 cut(s) 14, 830, 947
AlwI GGATC 1 cut(s) 171
AoxI GGCC 6 cut(s) 485, 573, 591, 652, 678, 984
ApeKI GCWGC 3 cut(s) 234, 417, 818
ApoI RAATTY 1 cut(s) 780
AsiGI ACCGGT 1 cut(s) 797
AspS9I GGNCC 5 cut(s) 77, 511, 556, 652, 678
AsuC2I CCSGG 2 cut(s) 161, 633
AsuHPI GGTGA 3 cut(s) 308, 346, 494
AvaII GGWCC 3 cut(s) 77, 511, 556
BbsI GAAGAC 2 cut(s) 537, 555
BbvI GCAGC 3 cut(s) 246, 429, 830
BccI CCATC 9 cut(s) 37, 93, 170, 192, 246, 252, 293, 439, 680
BceAI ACGGC 1 cut(s) 665
BciT130I CCWGG 4 cut(s) 82, 506, 590, 656
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 2 cut(s) 161, 633
BcoDI GTCTC 3 cut(s) 14, 830, 947
BfaI CTAG 3 cut(s) 560, 822, 917
BglII AGATCT 1 cut(s) 267
BisI GCNGC 6 cut(s) 235, 418, 608, 819, 902, 984
BlsI GCNGC 6 cut(s) 236, 419, 609, 820, 903, 985
Bme1390I CCNGG 6 cut(s) 82, 161, 506, 590, 633, 656
Bme18I GGWCC 3 cut(s) 77, 511, 556
BmgBI CACGTC 1 cut(s) 579
BmgT120I GGNCC 5 cut(s) 77, 511, 556, 652, 678
BmiI GGNNCC 6 cut(s) 78, 79, 155, 637, 670, 679
BmrFI CCNGG 6 cut(s) 82, 161, 506, 590, 633, 656
BmrI ACTGGG 3 cut(s) 82, 302, 659
BmsI GCATC 2 cut(s) 366, 805
BmuI ACTGGG 3 cut(s) 82, 302, 659
BpiI GAAGAC 2 cut(s) 537, 555
BpmI CTGGAG 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 161, 633
BsaJI CCNNGG 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 797
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 520, 632, 835
Bse118I RCCGGY 3 cut(s) 710, 797, 986
Bse1I ACTGG 3 cut(s) 77, 297, 654
BseBI CCWGG 4 cut(s) 82, 506, 590, 656
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 2 cut(s) 429, 820
BseLI CCNNNNNNNGG 3 cut(s) 520, 632, 835
BseNI ACTGG 3 cut(s) 77, 297, 654
BseRI GAGGAG 2 cut(s) 6, 756
BseX3I CGGCCG 1 cut(s) 984
BseXI GCAGC 3 cut(s) 246, 429, 830
Bsh1285I CGRYCG 1 cut(s) 987
BshFI GGCC 6 cut(s) 487, 575, 593, 654, 680, 986
BshTI ACCGGT 1 cut(s) 797
BsiEI CGRYCG 1 cut(s) 987
BsiSI CCGG 6 cut(s) 160, 632, 711, 770, 798, 987
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 3 cut(s) 520, 632, 835
BsmAI GTCTC 3 cut(s) 14, 830, 947
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 6 cut(s) 487, 575, 593, 654, 680, 986
Bsp143I GATC 4 cut(s) 163, 267, 336, 715
BspANI GGCC 6 cut(s) 487, 575, 593, 654, 680, 986
BspLI GGNNCC 6 cut(s) 78, 79, 155, 637, 670, 679
BspPI GGATC 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 57
BsrFI RCCGGY 3 cut(s) 710, 797, 986
BsrI ACTGG 3 cut(s) 77, 297, 654
BssAI RCCGGY 3 cut(s) 710, 797, 986
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 4 cut(s) 163, 267, 336, 715
Bst2UI CCWGG 4 cut(s) 82, 506, 590, 656
Bst4CI ACNGT 5 cut(s) 211, 430, 492, 847, 859
Bst6I CTCTTC 1 cut(s) 370
BstC8I GCNNGC 5 cut(s) 712, 841, 906, 910, 988
BstEII GGTNACC 1 cut(s) 495
BstF5I GGATG 2 cut(s) 429, 820
BstKTI GATC 4 cut(s) 166, 270, 339, 718
BstMAI GTCTC 3 cut(s) 14, 830, 947
BstMBI GATC 4 cut(s) 163, 267, 336, 715
BstMCI CGRYCG 1 cut(s) 987
BstMWI GCNNNNNNNGC 4 cut(s) 32, 417, 770, 983
BstNI CCWGG 4 cut(s) 82, 506, 590, 656
BstNSI RCATGY 1 cut(s) 843
BstPI GGTNACC 1 cut(s) 495
BstSCI CCNGG 6 cut(s) 80, 159, 504, 588, 631, 654
BstV1I GCAGC 3 cut(s) 246, 429, 830
BstV2I GAAGAC 2 cut(s) 537, 555
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BstZI CGGCCG 1 cut(s) 984
BsuRI GGCC 6 cut(s) 487, 575, 593, 654, 680, 986
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 1 cut(s) 579
BtsCI GGATG 2 cut(s) 429, 820
BtsIMutI CAGTG 3 cut(s) 497, 581, 843
Cac8I GCNNGC 5 cut(s) 712, 841, 906, 910, 988
Cfr10I RCCGGY 3 cut(s) 710, 797, 986
Cfr13I GGNCC 5 cut(s) 77, 511, 556, 652, 678
Csp6I GTAC 1 cut(s) 518
CspAI ACCGGT 1 cut(s) 797
CviAII CATG 2 cut(s) 147, 840
CviQI GTAC 1 cut(s) 518
DpnI GATC 4 cut(s) 165, 269, 338, 717
DpnII GATC 4 cut(s) 163, 267, 336, 715
EaeI YGGCCR 1 cut(s) 984
EagI CGGCCG 1 cut(s) 984
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
EciI GGCGGA 1 cut(s) 524
EclXI CGGCCG 1 cut(s) 984
Eco47I GGWCC 3 cut(s) 77, 511, 556
Eco52I CGGCCG 1 cut(s) 984
Eco57I CTGAAG 1 cut(s) 41
Eco91I GGTNACC 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 77, 556
EcoO65I GGTNACC 1 cut(s) 495
EcoRII CCWGG 4 cut(s) 80, 504, 588, 654
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 2 cut(s) 150, 843
FaiI YATR 8 cut(s) 148, 223, 232, 370, 750, 841, 944, 970
FaqI GGGAC 1 cut(s) 90
FatI CATG 2 cut(s) 146, 839
FauI CCCGC 2 cut(s) 50, 413
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 854, 874
Fnu4HI GCNGC 6 cut(s) 235, 418, 608, 819, 902, 984
FokI GGATG 2 cut(s) 436, 827
Fsp4HI GCNGC 6 cut(s) 235, 418, 608, 819, 902, 984
FspBI CTAG 3 cut(s) 560, 822, 917
GluI GCNGC 6 cut(s) 235, 418, 608, 819, 902, 984
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 6 cut(s) 487, 575, 593, 654, 680, 986
HapII CCGG 6 cut(s) 160, 632, 711, 770, 798, 987
Hin1II CATG 2 cut(s) 150, 843
HincII GTYRAC 4 cut(s) 207, 400, 855, 875
HindII GTYRAC 4 cut(s) 207, 400, 855, 875
HinfI GANTC 1 cut(s) 871
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 6 cut(s) 160, 632, 711, 770, 798, 987
HphI GGTGA 3 cut(s) 308, 346, 494
Hpy166II GTNNAC 4 cut(s) 207, 400, 855, 875
Hpy188I TCNGA 3 cut(s) 21, 42, 69
Hpy188III TCNNGA 5 cut(s) 281, 666, 865, 917, 925
Hpy8I GTNNAC 4 cut(s) 207, 400, 855, 875
Hpy99I CGWCG 1 cut(s) 871
HpyAV CCTTC 3 cut(s) 16, 751, 805
HpyCH4III ACNGT 5 cut(s) 211, 430, 492, 847, 859
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 6 cut(s) 89, 234, 417, 818, 843, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 417, 770, 983
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 2 cut(s) 150, 843
KflI GGGWCCC 1 cut(s) 77
KroI GCCGGC 2 cut(s) 710, 986
KroNI GCCGGC 2 cut(s) 712, 988
Ksp22I TGATCA 1 cut(s) 336
KspAI GTTAAC 1 cut(s) 400
Kzo9I GATC 4 cut(s) 163, 267, 336, 715
Lsp1109I GCAGC 3 cut(s) 246, 429, 830
LweI GCATC 2 cut(s) 366, 805
MaeI CTAG 3 cut(s) 560, 822, 917
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 4 cut(s) 495, 579, 645, 696
MalI GATC 4 cut(s) 165, 269, 338, 717
MbiI CCGCTC 1 cut(s) 57
MboI GATC 4 cut(s) 163, 267, 336, 715
MboII GAAGA 7 cut(s) 185, 261, 387, 542, 560, 776, 873
MfeI CAATTG 1 cut(s) 978
MflI RGATCY 1 cut(s) 267
MluCI AATT 3 cut(s) 308, 780, 978
MlyI GAGTC 1 cut(s) 880
MroNI GCCGGC 2 cut(s) 710, 986
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 3 cut(s) 237, 420, 607
MspI CCGG 6 cut(s) 160, 632, 711, 770, 798, 987
MspR9I CCNGG 6 cut(s) 82, 161, 506, 590, 633, 656
MunI CAATTG 1 cut(s) 978
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 4 cut(s) 82, 506, 590, 656
MwoI GCNNNNNNNGC 4 cut(s) 32, 417, 770, 983
NaeI GCCGGC 2 cut(s) 712, 988
NciI CCSGG 2 cut(s) 161, 633
NdeII GATC 4 cut(s) 163, 267, 336, 715
NgoMIV GCCGGC 2 cut(s) 710, 986
NlaIII CATG 2 cut(s) 150, 843
NlaIV GGNNCC 6 cut(s) 78, 79, 155, 637, 670, 679
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 2 cut(s) 579, 696
NspI RCATGY 1 cut(s) 843
PaeI GCATGC 1 cut(s) 843
PctI GAATGC 1 cut(s) 938
PdiI GCCGGC 2 cut(s) 712, 988
PflFI GACNNNGTC 1 cut(s) 554
PfoI TCCNGGA 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 797
PkrI GCNGC 6 cut(s) 236, 419, 609, 820, 903, 985
PleI GAGTC 1 cut(s) 879
PpsI GAGTC 1 cut(s) 879
PpuMI RGGWCCY 2 cut(s) 77, 556
Psp5II RGGWCCY 2 cut(s) 77, 556
Psp6I CCWGG 4 cut(s) 80, 504, 588, 654
PspEI GGTNACC 1 cut(s) 495
PspGI CCWGG 4 cut(s) 80, 504, 588, 654
PspN4I GGNNCC 6 cut(s) 78, 79, 155, 637, 670, 679
PspPI GGNCC 5 cut(s) 77, 511, 556, 652, 678
PspPPI RGGWCCY 2 cut(s) 77, 556
PsuI RGATCY 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 554
RsaI GTAC 1 cut(s) 519
RsaNI GTAC 1 cut(s) 518
SalI GTCGAC 2 cut(s) 853, 873
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 6 cut(s) 235, 418, 608, 819, 902, 984
Sau3AI GATC 4 cut(s) 163, 267, 336, 715
Sau96I GGNCC 5 cut(s) 77, 511, 556, 652, 678
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 6 cut(s) 82, 161, 506, 590, 633, 656
SfaNI GCATC 2 cut(s) 366, 805
SinI GGWCC 3 cut(s) 77, 511, 556
SphI GCATGC 1 cut(s) 843
Sse9I AATT 3 cut(s) 308, 780, 978
SspI AATATT 1 cut(s) 894
SspMI CTAG 3 cut(s) 560, 822, 917
StyD4I CCNGG 6 cut(s) 80, 159, 504, 588, 631, 654
TaaI ACNGT 5 cut(s) 211, 430, 492, 847, 859
TaiI ACGT 1 cut(s) 581
TaqI TCGA 7 cut(s) 215, 280, 373, 570, 854, 874, 924
TasI AATT 3 cut(s) 308, 780, 978
TauI GCSGC 3 cut(s) 610, 904, 986
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 3 cut(s) 497, 588, 850
TseFI GTSAC 2 cut(s) 579, 696
TseI GCWGC 3 cut(s) 234, 417, 818
Tsp45I GTSAC 2 cut(s) 579, 696
TspDTI ATGAA 1 cut(s) 931
TspRI CASTG 3 cut(s) 497, 588, 850
Tth111I GACNNNGTC 1 cut(s) 554
VpaK11BI GGWCC 3 cut(s) 77, 511, 556
XapI RAATTY 1 cut(s) 780
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 1 cut(s) 843
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 2 cut(s) 854, 874
XspI CTAG 3 cut(s) 560, 822, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.