Rh3DG283200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0014846
Physical Location & Seq
Forward (+)
28852358 .. 28856251
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG283200.1

Sequence Viewer

Length: 699 bp
ATGGTAAGACTCGTTCGAATTCGAGCGGATAATGGTGACGGAGCCGACGACGACGTCGCCGTGGTGGTACTGGAGAGGCAAAAGCTTCTGGAGCAAGAGGTCGTAGCCGTTCGGTCGCTGAAGCCTCAGGTCTTCTCGTTCCAGATGCGAATCGGAGTTGCCGATTTCCCATGGATCAATTGTTTCATGGTACCCTTTACCGGCAGGCTTCGTCTCTCTCCTCTGAAGGCGGTGTGGGCTATTCTGTTGGCTGGATCGAGCGGGGCTTCTCTGAACTGGGGACCCAGGTTTGCAGTTTGGAGATGGGGTTGCAATTGGGTTGAGCTTGACATTCGTTGTAGCGGTATTACATGGTGGAACCTCCGGGATCAGCAAGAAGATGGGGCAATACATCAGGGCAGATGGTGGTTGACGGTTTCGAGCCTATGGAGGCTATGCAGCGGTGTTTTTGGAAGATGGCGATGGCTGGAGACCTTGTGGGTTCGAGATCTGAAACTAGGTGATGGGGTTGAATTGATGTTTTGGGTCTTTGGATGGGTGATCACCGGTGGTTGGGTTGGCATCAAGTTGCTATGTCGAAGAGGGCGGTGGTCGTGTTTCGCTAACCCCCAATGCTCTTGCAGCGGGATGACGGTGGCCATCGGGCAGATGGAGGCGGCGGTCTTGGAGAGGATAGTTGTTCGTGGTAGAGTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

26.55

Weight (kDa)

9.57

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 190
AccBSI CCGCTC 2 cut(s) 26, 261
AciI CCGC 9 cut(s) 26, 230, 261, 342, 441, 586, 624, 656, 659
AclWI GGATC 3 cut(s) 182, 262, 375
AcoI YGGCCR 1 cut(s) 636
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 2 cut(s) 140, 245
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 69, 192
AfiI CCNNNNNNNGG 2 cut(s) 200, 552
AgeI ACCGGT 1 cut(s) 545
AgsI TTSAA 1 cut(s) 512
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 2 cut(s) 85, 325
AluI AGCT 2 cut(s) 85, 325
Alw26I GTCTC 2 cut(s) 218, 464
AlwI GGATC 3 cut(s) 182, 262, 375
AoxI GGCC 1 cut(s) 636
ApeKI GCWGC 2 cut(s) 438, 621
ApoI RAATTY 1 cut(s) 18
AsiGI ACCGGT 1 cut(s) 545
Asp718I GGTACC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 4 cut(s) 47, 512, 535, 550
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 281
AxyI CCTNAGG 1 cut(s) 126
BalI TGGCCA 1 cut(s) 638
BanI GGYRCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 450, 633
BccI CCATC 9 cut(s) 297, 374, 396, 450, 456, 497, 528, 643, 647
BceAI ACGGC 2 cut(s) 44, 92
BciT130I CCWGG 1 cut(s) 286
BclI TGATCA 1 cut(s) 540
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 218, 464
BfaI CTAG 1 cut(s) 497
BglII AGATCT 1 cut(s) 487
BisI GCNGC 3 cut(s) 439, 622, 657
BlsI GCNGC 3 cut(s) 440, 623, 658
Bme1390I CCNGG 2 cut(s) 286, 365
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 5 cut(s) 43, 192, 282, 283, 359
BmrFI CCNGG 2 cut(s) 286, 365
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 135, 570
BmuI ACTGGG 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 3 cut(s) 92, 110, 488
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 149
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 60, 170, 284
BsaWI WCCGGW 1 cut(s) 545
BsaXI ACNNNNNCTCC 4 cut(s) 292, 322, 461, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 552
Bse118I RCCGGY 2 cut(s) 200, 545
Bse1I ACTGG 2 cut(s) 75, 281
Bse21I CCTNAGG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 149
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 3 cut(s) 60, 170, 284
BseGI GGATG 2 cut(s) 539, 633
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 200, 552
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 75, 281
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 450, 633
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 1 cut(s) 638
BshNI GGYRCC 1 cut(s) 190
BshTI ACCGGT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 201, 364, 546
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 200, 552
BsmAI GTCTC 2 cut(s) 218, 464
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 1 cut(s) 638
Bso31I GGTCTC 1 cut(s) 464
Bsp119I TTCGAA 1 cut(s) 16
Bsp143I GATC 5 cut(s) 174, 254, 367, 487, 540
Bsp19I CCATGG 1 cut(s) 170
BspACI CCGC 9 cut(s) 26, 230, 261, 342, 441, 586, 624, 656, 659
BspANI GGCC 1 cut(s) 638
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 5 cut(s) 43, 192, 282, 283, 359
BspPI GGATC 3 cut(s) 182, 262, 375
BspT104I TTCGAA 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 464
BsrBI CCGCTC 2 cut(s) 26, 261
BsrFI RCCGGY 2 cut(s) 200, 545
BsrI ACTGG 2 cut(s) 75, 281
BssAI RCCGGY 2 cut(s) 200, 545
BssECI CCNNGG 3 cut(s) 60, 170, 284
BssMI GATC 5 cut(s) 174, 254, 367, 487, 540
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 415, 634
Bst6I CTCTTC 1 cut(s) 574
BstACI GRCGYC 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 126
BstDSI CCRYGG 2 cut(s) 60, 170
BstF5I GGATG 2 cut(s) 539, 633
BstKTI GATC 5 cut(s) 177, 257, 370, 490, 543
BstMAI GTCTC 2 cut(s) 218, 464
BstMBI GATC 5 cut(s) 174, 254, 367, 487, 540
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 3 cut(s) 91, 236, 621
BstNI CCWGG 1 cut(s) 286
BstSCI CCNGG 2 cut(s) 284, 363
BstV1I GCAGC 2 cut(s) 450, 633
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 126
BsuRI GGCC 1 cut(s) 638
BtgI CCRYGG 2 cut(s) 60, 170
BtgZI GCGATG 1 cut(s) 475
BtsCI GGATG 2 cut(s) 539, 633
Cac8I GCNNGC 1 cut(s) 206
Cfr10I RCCGGY 2 cut(s) 200, 545
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 68, 191
CspAI ACCGGT 1 cut(s) 545
CviAII CATG 3 cut(s) 171, 187, 351
CviQI GTAC 2 cut(s) 68, 191
DdeI CTNAG 1 cut(s) 126
DpnI GATC 5 cut(s) 176, 256, 369, 489, 542
DpnII GATC 5 cut(s) 174, 254, 367, 487, 540
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 636
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
Eco130I CCWWGG 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 1 cut(s) 281
Eco57I CTGAAG 2 cut(s) 140, 245
Eco81I CCTNAGG 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 3 cut(s) 174, 190, 354
FaiI YATR 6 cut(s) 172, 188, 352, 427, 436, 574
FaqI GGGAC 1 cut(s) 294
FatI CATG 3 cut(s) 170, 186, 350
FauI CCCGC 2 cut(s) 254, 617
FbaI TGATCA 1 cut(s) 540
Fnu4HI GCNGC 3 cut(s) 439, 622, 657
FokI GGATG 2 cut(s) 546, 640
Fsp4HI GCNGC 3 cut(s) 439, 622, 657
FspBI CTAG 1 cut(s) 497
GluI GCNGC 3 cut(s) 439, 622, 657
GsuI CTGGAG 3 cut(s) 92, 110, 488
HaeIII GGCC 1 cut(s) 638
HapII CCGG 3 cut(s) 201, 364, 546
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 174, 190, 354
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 2 cut(s) 9, 150
HpaII CCGG 3 cut(s) 201, 364, 546
HphI GGTGA 4 cut(s) 47, 512, 535, 550
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 4 cut(s) 155, 225, 273, 492
Hpy188III TCNNGA 3 cut(s) 89, 142, 485
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 4 cut(s) 50, 53, 56, 59
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 415, 634
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 4 cut(s) 293, 312, 438, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 236, 621
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 174, 190, 354
KflI GGGWCCC 1 cut(s) 281
KpnI GGTACC 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 5 cut(s) 174, 254, 367, 487, 540
LmnI GCTCC 2 cut(s) 41, 91
Lsp1109I GCAGC 2 cut(s) 450, 633
LweI GCATC 2 cut(s) 135, 570
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 35
MalI GATC 5 cut(s) 176, 256, 369, 489, 542
MbiI CCGCTC 2 cut(s) 26, 261
MboI GATC 5 cut(s) 174, 254, 367, 487, 540
MboII GAAGA 4 cut(s) 124, 389, 465, 591
MfeI CAATTG 2 cut(s) 178, 313
MflI RGATCY 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 638
MluCI AATT 4 cut(s) 18, 178, 313, 512
MluNI TGGCCA 1 cut(s) 638
MlyI GAGTC 1 cut(s) 3
MnlI CCTC 9 cut(s) 69, 91, 135, 231, 371, 423, 575, 646, 663
Mox20I TGGCCA 1 cut(s) 638
MscI TGGCCA 1 cut(s) 638
MseI TTAA 1 cut(s) 693
Msp20I TGGCCA 1 cut(s) 638
MspA1I CMGCKG 2 cut(s) 441, 624
MspI CCGG 3 cut(s) 201, 364, 546
MspR9I CCNGG 2 cut(s) 286, 365
MunI CAATTG 2 cut(s) 178, 313
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 91, 236, 621
NciI CCSGG 1 cut(s) 365
NcoI CCATGG 1 cut(s) 170
NdeII GATC 5 cut(s) 174, 254, 367, 487, 540
NlaIII CATG 3 cut(s) 174, 190, 354
NlaIV GGNNCC 5 cut(s) 43, 192, 282, 283, 359
NmuCI GTSAC 1 cut(s) 35
NspV TTCGAA 1 cut(s) 16
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 57, 159
PfeI GAWTC 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 363
PinAI ACCGGT 1 cut(s) 545
PkrI GCNGC 3 cut(s) 440, 623, 658
PleI GAGTC 1 cut(s) 3
PpsI GAGTC 1 cut(s) 3
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 5 cut(s) 43, 192, 282, 283, 359
PspPI GGNCC 1 cut(s) 281
PspPPI RGGWCCY 1 cut(s) 281
PsrI GAACNNNNNNTAC 1 cut(s) 29
PsuI RGATCY 1 cut(s) 487
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 2 cut(s) 69, 192
RsaNI GTAC 2 cut(s) 68, 191
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 3 cut(s) 439, 622, 657
Sau3AI GATC 5 cut(s) 174, 254, 367, 487, 540
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 1 cut(s) 3
ScrFI CCNGG 2 cut(s) 286, 365
SetI ASST 9 cut(s) 57, 87, 102, 132, 290, 327, 363, 476, 502
SfaNI GCATC 2 cut(s) 135, 570
SfuI TTCGAA 1 cut(s) 16
SgrAI CRCCGGYG 1 cut(s) 545
SinI GGWCC 1 cut(s) 281
Sse9I AATT 4 cut(s) 18, 178, 313, 512
SsiI CCGC 9 cut(s) 26, 230, 261, 342, 441, 586, 624, 656, 659
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 284, 363
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 415, 634
TaiI ACGT 1 cut(s) 57
TaqI TCGA 6 cut(s) 16, 22, 257, 419, 484, 577
TaqII GACCGA 1 cut(s) 102
TasI AATT 4 cut(s) 18, 178, 313, 512
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 438, 621
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 175
TspGWI ACGGA 1 cut(s) 54
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 1 cut(s) 18
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XspI CTAG 1 cut(s) 497
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.