Rorug07G0205600

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004641
Physical Location & Seq
Reverse (-)
18066601 .. 18070770
4170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0205600.1

Sequence Viewer

Length: 990 bp
ATGACGCGGCGGTGCTCGCATTGCAGCCACAATGGCCACAACTCCCGCACGTGCCCCAACCGCGGCGTCAAGCTCTTCGGCGTCCGCCTCACCGACGGCGGCATCAGGAAGAGTGCTAGTATGGGCAATCTGACCCACTATGCCGGGTCCGGTTCGGGTCTGCTCCATAACAACCCGGACTCTCCCGGCGAGGCTGCTGATCACTCCGGCGCCGCTGACGGTTATGCTTCTGAGGACTTCGTGCCCGGATCGTCTTCCAGCTGCCGGGAACGTAAAAAAGGCACCCCATGGACAGAGGAGGAACATAGGATGTTTTTACTTGGACTACAGAAACTTGGTAAAGGTGATTGGCGTGGAATCGCTCGCAATTATGTTATTTCAAGAACACCTACTCAAGTGGCTAGCCATGCTCAGAAATATTTCATTAGGCAGTCTAATGTATCACGGAGAAAAAGACGTTCTAGCCTGTTTGATATAGTAGCAGATGATTCAGTTGATACTCCAATGGAACCACTGGACTTCTTACCTGAAAACCATGTAGAAGATGATACTGAAAGCAACCACCCATTGCCTGCTCCTCCAGCTTTGGATGAAGAATGTGAATCAATGGCATCTACCAACTCAGATGGAGAACCTTCCCCTCCAAAGGCTGATAACTCACAGTCATGTTATCCAGTGATGTATCCCACTTATTTCTCACCTTTTCCATTTCCTTTTCCAATGTGGCCTGGATACAGTACTGAGCCAACGAAGGTGGATACACATGAGGTCCTTAAGCCAACGGCAGTACATTCAAAGAGCCCAATTAATGTTGATGAGCTAGTAGGCATGTCGAATCTGAGTATAGGAGAGCCTAAAGGTCAAGCTGGTCCGTCCTCGCTCTCACTAAATCTTGTTGAAGGGTCCTCTAGGCAATCGGCTTTCCATGCAAATCCAAGTTCTGGCAGTTCAAATATGAAATCAGGTGGTAGTCCAATCCATGCGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

329

Amino Acids

35.61

Weight (kDa)

6.27

Isoelectric Point (pI)

60.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 96 - 141 2.2e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 281
AccB7I CCANNNNNTGG 1 cut(s) 941
AccII CGCG 2 cut(s) 7, 63
AciI CCGC 8 cut(s) 7, 10, 46, 61, 63, 85, 99, 213
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 34
AcvI CACGTG 1 cut(s) 51
AcyI GRCGYC 3 cut(s) 66, 81, 210
AfaI GTAC 2 cut(s) 739, 789
AfiI CCNNNNNNNGG 4 cut(s) 62, 264, 646, 941
AflII CTTAAG 1 cut(s) 773
AgsI TTSAA 4 cut(s) 381, 795, 899, 951
AjnI CCWGG 1 cut(s) 727
AluBI AGCT 5 cut(s) 73, 261, 584, 820, 866
AluI AGCT 5 cut(s) 73, 261, 584, 820, 866
Alw21I GWGCWC 1 cut(s) 17
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 34, 725
ApeKI GCWGC 3 cut(s) 24, 194, 261
AseI ATTAAT 1 cut(s) 807
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 212, 985
AspS9I GGNCC 4 cut(s) 147, 769, 869, 903
AsuC2I CCSGG 5 cut(s) 145, 176, 186, 246, 266
AsuHPI GGTGA 3 cut(s) 82, 356, 690
AsuNHI GCTAGC 1 cut(s) 401
AvaII GGWCC 4 cut(s) 147, 769, 869, 903
BaeGI GKGCMC 2 cut(s) 56, 246
BalI TGGCCA 1 cut(s) 36
BanI GGYRCC 2 cut(s) 209, 281
BanII GRGCYC 1 cut(s) 803
BbrPI CACGTG 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 36, 181, 248
BccI CCATC 1 cut(s) 620
BceAI ACGGC 2 cut(s) 112, 798
BciT130I CCWGG 1 cut(s) 729
BciVI GTATCC 3 cut(s) 693, 725, 751
BclI TGATCA 1 cut(s) 199
BcnI CCSGG 5 cut(s) 145, 176, 186, 246, 266
BfaI CTAG 5 cut(s) 117, 402, 462, 821, 909
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 773
BfuI GTATCC 3 cut(s) 693, 725, 751
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 7 cut(s) 8, 25, 64, 100, 195, 213, 262
BlsI GCNGC 7 cut(s) 9, 26, 65, 101, 196, 214, 263
BmcAI AGTACT 1 cut(s) 739
Bme1390I CCNGG 6 cut(s) 145, 176, 186, 246, 266, 729
Bme18I GGWCC 4 cut(s) 147, 769, 869, 903
BmgT120I GGNCC 4 cut(s) 147, 769, 869, 903
BmiI GGNNCC 5 cut(s) 148, 211, 283, 510, 904
BmrFI CCNGG 6 cut(s) 145, 176, 186, 246, 266, 729
BmsI GCATC 2 cut(s) 111, 620
BmtI GCTAGC 1 cut(s) 405
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 5 cut(s) 145, 176, 186, 246, 266
BsaAI YACGTR 1 cut(s) 51
BsaHI GRCGYC 3 cut(s) 66, 81, 210
BsaJI CCNNGG 2 cut(s) 61, 287
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 264, 646, 941
Bse1I ACTGG 2 cut(s) 519, 674
Bse3DI GCAATG 2 cut(s) 19, 566
BseBI CCWGG 1 cut(s) 729
BseDI CCNNGG 2 cut(s) 61, 287
BseGI GGATG 2 cut(s) 315, 595
BseLI CCNNNNNNNGG 4 cut(s) 62, 264, 646, 941
BseMI GCAATG 2 cut(s) 19, 566
BseMII CTCAG 5 cut(s) 222, 425, 636, 732, 830
BseNI ACTGG 2 cut(s) 519, 674
BseRI GAGGAG 2 cut(s) 311, 567
BseSI GKGCMC 2 cut(s) 56, 246
BseXI GCAGC 3 cut(s) 36, 181, 248
Bsh1236I CGCG 2 cut(s) 7, 63
BshFI GGCC 2 cut(s) 36, 727
BshNI GGYRCC 2 cut(s) 209, 281
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 7 cut(s) 144, 150, 176, 186, 207, 246, 265
BslI CCNNNNNNNGG 4 cut(s) 62, 264, 646, 941
BsnI GGCC 2 cut(s) 36, 727
Bsp1286I GDGCHC 4 cut(s) 17, 56, 246, 803
Bsp143I GATC 2 cut(s) 199, 248
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 8 cut(s) 7, 10, 46, 61, 63, 85, 99, 213
BspANI GGCC 2 cut(s) 36, 727
BspCNI CTCAG 5 cut(s) 223, 424, 635, 733, 831
BspFNI CGCG 2 cut(s) 7, 63
BspLI GGNNCC 5 cut(s) 148, 211, 283, 510, 904
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 1 cut(s) 256
BspQI GCTCTTC 1 cut(s) 80
BspT107I GGYRCC 2 cut(s) 209, 281
BspTI CTTAAG 1 cut(s) 773
BsrDI GCAATG 2 cut(s) 19, 566
BsrI ACTGG 2 cut(s) 519, 674
BssECI CCNNGG 2 cut(s) 61, 287
BssMI GATC 2 cut(s) 199, 248
BssNI GRCGYC 3 cut(s) 66, 81, 210
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 729
Bst4CI ACNGT 3 cut(s) 221, 663, 737
Bst6I CTCTTC 2 cut(s) 80, 104
BstACI GRCGYC 3 cut(s) 66, 81, 210
BstAFI CTTAAG 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 4 cut(s) 17, 364, 403, 573
BstDEI CTNAG 5 cut(s) 231, 411, 622, 741, 839
BstDSI CCRYGG 2 cut(s) 61, 287
BstF5I GGATG 2 cut(s) 315, 595
BstFNI CGCG 2 cut(s) 7, 63
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 212, 985
BstKTI GATC 2 cut(s) 202, 251
BstMBI GATC 2 cut(s) 199, 248
BstMWI GCNNNNNNNGC 7 cut(s) 16, 21, 33, 60, 407, 581, 926
BstNI CCWGG 1 cut(s) 729
BstNSI RCATGY 1 cut(s) 832
BstSCI CCNGG 6 cut(s) 143, 174, 184, 244, 264, 727
BstSFI CTRYAG 1 cut(s) 326
BstSLI GKGCMC 2 cut(s) 56, 246
BstUI CGCG 2 cut(s) 7, 63
BstV1I GCAGC 3 cut(s) 36, 181, 248
BstV2I GAAGAC 1 cut(s) 246
BsuI GTATCC 3 cut(s) 693, 725, 751
BsuRI GGCC 2 cut(s) 36, 727
BtgI CCRYGG 2 cut(s) 61, 287
BtsCI GGATG 2 cut(s) 315, 595
BtsIMutI CAGTG 2 cut(s) 512, 681
Cac8I GCNNGC 4 cut(s) 17, 364, 403, 573
CfoI GCGC 2 cut(s) 212, 985
Cfr13I GGNCC 4 cut(s) 147, 769, 869, 903
Cfr42I CCGCGG 1 cut(s) 64
CseI GACGC 3 cut(s) 13, 55, 70
Csp6I GTAC 2 cut(s) 738, 788
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviAII CATG 8 cut(s) 288, 407, 536, 666, 764, 829, 926, 980
CviQI GTAC 2 cut(s) 738, 788
DdeI CTNAG 5 cut(s) 231, 411, 622, 741, 839
DinI GGCGCC 1 cut(s) 211
DpnI GATC 2 cut(s) 201, 250
DpnII GATC 2 cut(s) 199, 248
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 80, 104
EarI CTCTTC 2 cut(s) 80, 104
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 803
Eco47I GGWCC 4 cut(s) 147, 769, 869, 903
Eco72I CACGTG 1 cut(s) 51
EcoO109I RGGNCCY 2 cut(s) 769, 903
EcoRII CCWGG 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 8 cut(s) 291, 410, 539, 669, 767, 832, 929, 983
FatI CATG 8 cut(s) 287, 406, 535, 665, 763, 828, 925, 979
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 7 cut(s) 8, 25, 64, 100, 195, 213, 262
FokI GGATG 2 cut(s) 322, 602
FriOI GRGCYC 1 cut(s) 803
Fsp4HI GCNGC 7 cut(s) 8, 25, 64, 100, 195, 213, 262
FspBI CTAG 5 cut(s) 117, 402, 462, 821, 909
GlaI GCGC 2 cut(s) 211, 984
GluI GCNGC 7 cut(s) 8, 25, 64, 100, 195, 213, 262
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 2 cut(s) 36, 727
HapII CCGG 7 cut(s) 144, 150, 176, 186, 207, 246, 265
HgaI GACGC 3 cut(s) 13, 55, 70
HhaI GCGC 2 cut(s) 212, 985
Hin1I GRCGYC 3 cut(s) 66, 81, 210
Hin1II CATG 8 cut(s) 291, 410, 539, 669, 767, 832, 929, 983
Hin6I GCGC 2 cut(s) 210, 983
HinP1I GCGC 2 cut(s) 210, 983
HinfI GANTC 5 cut(s) 179, 357, 488, 602, 835
HpaII CCGG 7 cut(s) 144, 150, 176, 186, 207, 246, 265
HphI GGTGA 3 cut(s) 82, 356, 690
Hpy188I TCNGA 5 cut(s) 132, 232, 414, 625, 840
Hpy188III TCNNGA 2 cut(s) 106, 381
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 645, 745, 893
HpyCH4III ACNGT 3 cut(s) 221, 663, 737
HpyCH4IV ACGT 3 cut(s) 50, 271, 457
HpyCH4V TGCA 2 cut(s) 24, 929
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 21, 33, 60, 407, 581, 926
HpyF3I CTNAG 5 cut(s) 231, 411, 622, 741, 839
HpySE526I ACGT 3 cut(s) 50, 271, 457
Hsp92I GRCGYC 3 cut(s) 66, 81, 210
Hsp92II CATG 8 cut(s) 291, 410, 539, 669, 767, 832, 929, 983
HspAI GCGC 2 cut(s) 210, 983
KasI GGCGCC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 199
KspI CCGCGG 1 cut(s) 64
Kzo9I GATC 2 cut(s) 199, 248
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 2 cut(s) 168, 580
Lsp1109I GCAGC 3 cut(s) 36, 181, 248
LweI GCATC 2 cut(s) 111, 620
MaeI CTAG 5 cut(s) 117, 402, 462, 821, 909
MaeII ACGT 3 cut(s) 50, 271, 457
MalI GATC 2 cut(s) 201, 250
MboI GATC 2 cut(s) 199, 248
MboII GAAGA 5 cut(s) 67, 121, 246, 554, 605
MhlI GDGCHC 4 cut(s) 17, 56, 246, 803
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 2 cut(s) 367, 804
MluNI TGGCCA 1 cut(s) 36
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 173
Mox20I TGGCCA 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 36
MseI TTAA 2 cut(s) 774, 807
MslI CAYNNNNRTG 1 cut(s) 664
Msp20I TGGCCA 1 cut(s) 36
MspA1I CMGCKG 3 cut(s) 63, 215, 261
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 7 cut(s) 144, 150, 176, 186, 207, 246, 265
MspR9I CCNGG 6 cut(s) 145, 176, 186, 246, 266, 729
MvaI CCWGG 1 cut(s) 729
MvnI CGCG 2 cut(s) 7, 63
MwoI GCNNNNNNNGC 7 cut(s) 16, 21, 33, 60, 407, 581, 926
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 5 cut(s) 145, 176, 186, 246, 266
NcoI CCATGG 1 cut(s) 287
NdeII GATC 2 cut(s) 199, 248
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 8 cut(s) 291, 410, 539, 669, 767, 832, 929, 983
NlaIV GGNNCC 5 cut(s) 148, 211, 283, 510, 904
NspI RCATGY 1 cut(s) 832
PciSI GCTCTTC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 4 cut(s) 357, 488, 602, 835
PflMI CCANNNNNTGG 1 cut(s) 941
PkrI GCNGC 7 cut(s) 9, 26, 65, 101, 196, 214, 263
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 1 cut(s) 213
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 51
PpuMI RGGWCCY 2 cut(s) 769, 903
PshBI ATTAAT 1 cut(s) 807
Psp5II RGGWCCY 2 cut(s) 769, 903
Psp6I CCWGG 1 cut(s) 727
PspCI CACGTG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 727
PspN4I GGNNCC 5 cut(s) 148, 211, 283, 510, 904
PspPI GGNCC 4 cut(s) 147, 769, 869, 903
PspPPI RGGWCCY 2 cut(s) 769, 903
PvuII CAGCTG 1 cut(s) 261
RsaI GTAC 2 cut(s) 739, 789
RsaNI GTAC 2 cut(s) 738, 788
RseI CAYNNNNRTG 1 cut(s) 664
SacII CCGCGG 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 2 cut(s) 774, 807
SatI GCNGC 7 cut(s) 8, 25, 64, 100, 195, 213, 262
Sau3AI GATC 2 cut(s) 199, 248
Sau96I GGNCC 4 cut(s) 147, 769, 869, 903
ScaI AGTACT 1 cut(s) 739
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 6 cut(s) 145, 176, 186, 246, 266, 729
SduI GDGCHC 4 cut(s) 17, 56, 246, 803
SfaNI GCATC 2 cut(s) 111, 620
SfcI CTRYAG 1 cut(s) 326
SfoI GGCGCC 1 cut(s) 211
Sfr303I CCGCGG 1 cut(s) 64
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 4 cut(s) 147, 769, 869, 903
SmiMI CAYNNNNRTG 1 cut(s) 664
SmlI CTYRAG 2 cut(s) 393, 773
SmoI CTYRAG 2 cut(s) 393, 773
Sse9I AATT 2 cut(s) 367, 804
SsiI CCGC 8 cut(s) 7, 10, 46, 61, 63, 85, 99, 213
SspDI GGCGCC 1 cut(s) 209
SspI AATATT 1 cut(s) 419
SspMI CTAG 5 cut(s) 117, 402, 462, 821, 909
StyD4I CCNGG 6 cut(s) 143, 174, 184, 244, 264, 727
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 3 cut(s) 221, 663, 737
TaiI ACGT 3 cut(s) 53, 274, 460
TaqI TCGA 1 cut(s) 833
TasI AATT 2 cut(s) 367, 804
TatI WGTACW 2 cut(s) 737, 787
TauI GCSGC 4 cut(s) 10, 66, 102, 215
TfiI GAWTC 4 cut(s) 357, 488, 602, 835
Tru1I TTAA 2 cut(s) 774, 807
Tru9I TTAA 2 cut(s) 774, 807
TscAI CASTG 2 cut(s) 519, 681
TseI GCWGC 3 cut(s) 24, 194, 261
TspDTI ATGAA 3 cut(s) 412, 606, 971
TspGWI ACGGA 2 cut(s) 460, 861
TspRI CASTG 2 cut(s) 519, 681
Van91I CCANNNNNTGG 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 773
VpaK11BI GGWCC 4 cut(s) 147, 769, 869, 903
VspI ATTAAT 1 cut(s) 807
XceI RCATGY 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 5 cut(s) 117, 402, 462, 821, 909
ZrmI AGTACT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.