AT1G71170

Contains the following InterPro domains 6-phosphogluconate dehydrogenase, NAD-binding (InterPro IPR006115), Dehydrogenase, multihelical (InterPro IPR013328), 6-phosphogluconate dehydrogenase, C-terminal-like (InterPro IPR008927), 3-hydroxyacid dehydrogenase reductase (InterPro IPR015815), NAD(P)-binding domain (InterPro IPR016040)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004641
Physical Location & Seq
Forward (+)
26830603 .. 26832123
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G71170.1

Sequence Viewer

Length: 900 bp
ATGGAGACTCAGTATCCGAAACTAATCGAACCATCCAAGACCCGAATTGGATGGATTGGGATCGGGATAATGGGATCAGCGATGGTTTCTCATATCCTCGCCGCAGGCTATTCAGTCACCGTTTATGCTCGCGATCTTCGTAAAACAAAGGATCTGCAAACCAAAGGAGGTCGTACCGCCAATAGCCCCAAAGAGTTAGGGGAGATGAGCGACGTTGTGTTCACGATCGTTGGAAACTCTAACGATGTTAGATCGTTGCTTCTTGGGGATGATGGTGTACTCTCTGGTCTTAAACCCGGTGGAGTAACAGTGGACATGACTAGTAGCAAGCCAGGATTGGCGCGTGAGATATACGCGGAGGCGAGGCGGAGGGATTGCTGGGCGGTGGATGCGCCTGTATCGGGTGGAGACGCTGGAGCACGTGAGGGGAAGCTAACGATATTCGCCGGCGGAGATTCCGAGATTGTGGAGTGGTTGGCTCCGGTGATGAAAACGATGGGAATTGTGAGGTTTATGGGAGGAGCTGGGAGTGGACAGAGCTGTAAAATTGGGAATCAGATTTGTGTTGGAAGCAACATGATTGGATTAGCTGAAGGGATTGTGTTTGCTGAGAAGGCGGGTTTAGATCCGGTGAAATGGCTTGAGGCGGTTAAGGACGGTGCAGCTGGATCGGCGGTTATGCGGCTTTTCGGGGAGATGATGGCGGTGAGGGACTATAAGGCGACGGGGTTTGCGGAGTATATGGTGAAAGATTTGGGAATGGCGGCGGAGGCGGCAATGGCGATGCCTGGAACTGCTTTGAACAAGCAGTTGTTCACTGTAATGGTGGCAAATGGAGATGGGAAGTTAGGATTTCAAGGCGTCGTCGATGTTATTAGGAGACTTAACGGCTTATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

299

Amino Acids

31.44

Weight (kDa)

7.63

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 169 2.4e-46 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 77 6.1e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 291 7.1e-24 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 132, 343, 356
AclWI GGATC 5 cut(s) 68, 82, 159, 620, 676
AcuI CTGAAG 1 cut(s) 612
AcvI CACGTG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 2 cut(s) 175, 279
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 2 cut(s) 802, 857
AhlI ACTAGT 1 cut(s) 320
AjnI CCWGG 2 cut(s) 331, 787
AluBI AGCT 5 cut(s) 433, 524, 540, 590, 665
AluI AGCT 5 cut(s) 433, 524, 540, 590, 665
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 2 cut(s) 402, 874
AlwI GGATC 5 cut(s) 68, 82, 159, 620, 676
ApeKI GCWGC 1 cut(s) 662
AspLEI GCGC 2 cut(s) 343, 394
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 5 cut(s) 109, 496, 643, 718, 757
BbrPI CACGTG 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 1 cut(s) 674
BccI CCATC 7 cut(s) 40, 45, 76, 266, 490, 694, 833
BcgI CGANNNNNNTGC 2 cut(s) 651, 685
BciT130I CCWGG 2 cut(s) 333, 789
BciVI GTATCC 1 cut(s) 24
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 402, 874
BcuI ACTAGT 1 cut(s) 320
BfaI CTAG 1 cut(s) 321
BfuI GTATCC 1 cut(s) 24
BisI GCNGC 5 cut(s) 102, 663, 683, 765, 774
BlsI GCNGC 5 cut(s) 103, 664, 684, 766, 775
Bme1390I CCNGG 3 cut(s) 297, 333, 789
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 3 cut(s) 297, 333, 789
BmsI GCATC 2 cut(s) 379, 774
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 861
BsaWI WCCGGW 2 cut(s) 481, 628
BsaXI ACNNNNNCTCC 3 cut(s) 26, 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 2 cut(s) 333, 789
BseGI GGATG 4 cut(s) 32, 56, 274, 394
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 2 cut(s) 23, 600
BseRI GAGGAG 1 cut(s) 534
BseXI GCAGC 1 cut(s) 674
BseYI CCCAGC 2 cut(s) 378, 524
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 3 cut(s) 132, 343, 356
Bsh1285I CGRYCG 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 4 cut(s) 297, 447, 482, 629
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 2 cut(s) 402, 874
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
Bsp68I TCGCGA 1 cut(s) 132
BspCNI CTCAG 2 cut(s) 22, 601
BspFNI CGCG 3 cut(s) 132, 343, 356
BspLI GGNNCC 1 cut(s) 480
BspPI GGATC 5 cut(s) 68, 82, 159, 620, 676
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 446
BssAI RCCGGY 1 cut(s) 446
BssMI GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
BssNI GRCGYC 1 cut(s) 861
Bst2UI CCWGG 2 cut(s) 333, 789
Bst4CI ACNGT 4 cut(s) 121, 310, 659, 820
BstACI GRCGYC 1 cut(s) 861
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 4 cut(s) 106, 130, 329, 448
BstDEI CTNAG 2 cut(s) 9, 609
BstF5I GGATG 4 cut(s) 32, 56, 274, 394
BstFNI CGCG 3 cut(s) 132, 343, 356
BstHHI GCGC 2 cut(s) 343, 394
BstKTI GATC 8 cut(s) 63, 77, 136, 154, 228, 254, 628, 671
BstMAI GTCTC 2 cut(s) 402, 874
BstMBI GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 6 cut(s) 389, 614, 671, 770, 773, 779
BstNI CCWGG 2 cut(s) 333, 789
BstSCI CCNGG 3 cut(s) 295, 331, 787
BstUI CGCG 3 cut(s) 132, 343, 356
BstV1I GCAGC 1 cut(s) 674
BstX2I RGATCY 2 cut(s) 151, 625
BstYI RGATCY 2 cut(s) 151, 625
BsuI GTATCC 1 cut(s) 24
BtgZI GCGATG 2 cut(s) 95, 797
BtsCI GGATG 4 cut(s) 32, 56, 274, 394
BtsIMutI CAGTG 2 cut(s) 315, 816
BtuMI TCGCGA 1 cut(s) 132
Cac8I GCNNGC 4 cut(s) 106, 130, 329, 448
CfoI GCGC 2 cut(s) 343, 394
Cfr10I RCCGGY 1 cut(s) 446
CseI GACGC 2 cut(s) 419, 850
Csp6I GTAC 2 cut(s) 174, 278
CviAII CATG 2 cut(s) 316, 577
CviQI GTAC 2 cut(s) 174, 278
DdeI CTNAG 2 cut(s) 9, 609
DpnI GATC 8 cut(s) 62, 76, 135, 153, 227, 253, 627, 670
DpnII GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
EciI GGCGGA 3 cut(s) 382, 465, 782
Eco57I CTGAAG 1 cut(s) 612
Eco72I CACGTG 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 331, 787
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 2 cut(s) 319, 580
FaqI GGGAC 1 cut(s) 725
FatI CATG 2 cut(s) 315, 576
FauI CCCGC 1 cut(s) 610
Fnu4HI GCNGC 5 cut(s) 102, 663, 683, 765, 774
FokI GGATG 4 cut(s) 19, 63, 281, 401
Fsp4HI GCNGC 5 cut(s) 102, 663, 683, 765, 774
FspBI CTAG 1 cut(s) 321
GlaI GCGC 2 cut(s) 342, 393
GluI GCNGC 5 cut(s) 102, 663, 683, 765, 774
GsaI CCCAGC 2 cut(s) 382, 528
GsuI CTGGAG 1 cut(s) 435
HapII CCGG 4 cut(s) 297, 447, 482, 629
HgaI GACGC 2 cut(s) 419, 850
HhaI GCGC 2 cut(s) 343, 394
Hin1I GRCGYC 1 cut(s) 861
Hin1II CATG 2 cut(s) 319, 580
Hin6I GCGC 2 cut(s) 341, 392
HinP1I GCGC 2 cut(s) 341, 392
HinfI GANTC 3 cut(s) 7, 455, 553
HpaII CCGG 4 cut(s) 297, 447, 482, 629
HphI GGTGA 5 cut(s) 109, 496, 643, 718, 757
Hpy166II GTNNAC 5 cut(s) 222, 278, 313, 533, 816
Hpy188I TCNGA 3 cut(s) 18, 460, 558
Hpy188III TCNNGA 4 cut(s) 64, 131, 223, 897
Hpy8I GTNNAC 5 cut(s) 222, 278, 313, 533, 816
Hpy99I CGWCG 4 cut(s) 215, 727, 866, 869
HpyAV CCTTC 2 cut(s) 587, 607
HpyCH4III ACNGT 4 cut(s) 121, 310, 659, 820
HpyCH4IV ACGT 2 cut(s) 213, 421
HpyCH4V TGCA 2 cut(s) 157, 662
HpyF10VI GCNNNNNNNGC 6 cut(s) 389, 614, 671, 770, 773, 779
HpyF3I CTNAG 2 cut(s) 9, 609
HpySE526I ACGT 2 cut(s) 213, 421
Hsp92I GRCGYC 1 cut(s) 861
Hsp92II CATG 2 cut(s) 319, 580
HspAI GCGC 2 cut(s) 341, 392
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
Kzo9I GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
LmnI GCTCC 3 cut(s) 416, 484, 521
Lsp1109I GCAGC 1 cut(s) 674
LweI GCATC 2 cut(s) 379, 774
MaeI CTAG 1 cut(s) 321
MaeII ACGT 2 cut(s) 213, 421
MaeIII GTNAC 2 cut(s) 115, 304
MalI GATC 8 cut(s) 62, 76, 135, 153, 227, 253, 627, 670
MboI GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
MboII GAAGA 1 cut(s) 128
MflI RGATCY 2 cut(s) 151, 625
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 3 cut(s) 45, 501, 546
MmeI TCCRAC 2 cut(s) 211, 547
MreI CGCCGGCG 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 446
MseI TTAA 3 cut(s) 291, 651, 885
MslI CAYNNNNRTG 1 cut(s) 821
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 4 cut(s) 297, 447, 482, 629
MspR9I CCNGG 3 cut(s) 297, 333, 789
MvaI CCWGG 2 cut(s) 333, 789
MvnI CGCG 3 cut(s) 132, 343, 356
MwoI GCNNNNNNNGC 6 cut(s) 389, 614, 671, 770, 773, 779
NaeI GCCGGC 1 cut(s) 448
NciI CCSGG 1 cut(s) 297
NdeII GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 2 cut(s) 319, 580
NlaIV GGNNCC 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PdiI GCCGGC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 455, 553
PkrI GCNGC 5 cut(s) 103, 664, 684, 766, 775
Ple19I CGATCG 1 cut(s) 228
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 331, 787
PspCI CACGTG 1 cut(s) 422
PspFI CCCAGC 2 cut(s) 378, 524
PspGI CCWGG 2 cut(s) 331, 787
PspN4I GGNNCC 1 cut(s) 480
PsuI RGATCY 2 cut(s) 151, 625
PvuI CGATCG 1 cut(s) 228
PvuII CAGCTG 1 cut(s) 665
RruI TCGCGA 1 cut(s) 132
RsaI GTAC 2 cut(s) 175, 279
RsaNI GTAC 2 cut(s) 174, 278
RseI CAYNNNNRTG 1 cut(s) 821
SaqAI TTAA 3 cut(s) 291, 651, 885
SatI GCNGC 5 cut(s) 102, 663, 683, 765, 774
Sau3AI GATC 8 cut(s) 60, 74, 133, 151, 225, 251, 625, 668
ScrFI CCNGG 3 cut(s) 297, 333, 789
SduI GDGCHC 1 cut(s) 421
SetI ASST 9 cut(s) 172, 216, 424, 435, 512, 526, 542, 592, 667
SfaNI GCATC 2 cut(s) 379, 774
SgrAI CRCCGGYG 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SpeI ACTAGT 1 cut(s) 320
Sse9I AATT 3 cut(s) 45, 501, 546
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 295, 331, 787
TaaI ACNGT 4 cut(s) 121, 310, 659, 820
TaiI ACGT 2 cut(s) 216, 424
TaqI TCGA 2 cut(s) 27, 867
TasI AATT 3 cut(s) 45, 501, 546
TatI WGTACW 1 cut(s) 277
TauI GCSGC 4 cut(s) 104, 685, 767, 776
TfiI GAWTC 2 cut(s) 455, 553
Tru1I TTAA 3 cut(s) 291, 651, 885
Tru9I TTAA 3 cut(s) 291, 651, 885
TscAI CASTG 2 cut(s) 315, 823
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 503
TspRI CASTG 2 cut(s) 315, 823
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.