FvH4_5g29271

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004641
Physical Location & Seq
Reverse (-)
20301338 .. 20302527
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29271.t1

Sequence Viewer

Length: 939 bp
ATGGGAACTCCTTACCCAAACCCCATTTCTCCGGCCAAAACCCGCATCGGCTGGATTGGCATCGGCGTTATGGGCGCTGCCATGGCCTCCCGCCTCCTCTCAGCTGGCTACTCCCTCACCGTCTACGCTCGCACTCCTTCAAAAGCCGCTCCCCTCCAATCCCAGGGCGCCCACCTCGCCGACTCCCCATTTCAACTCGCCCAACTCTCCGACGTCGTCTTCACCATGGTGGGCCACCCCTCCGATGTCCGATCCAACTACCTCTCCCCCAACGCCCTTCTTTCCAGCCTCAATCCCAATTCCGTCACCGTCGACATGACCAGCAGCCACCCGGCGCTCGCGCGTGAGATTTTCGCCGCCTCACGCGCCAAACACTGCTGGTCTGTCGACGCGCCGGTATCCGGCGGCGACATCGGCGCCAGGGAAGGCAAGCTTGCCATACTCGCCGGCGGGGATGCCTCGGTGGTGACGTGGCTGGCGCCCTTGTTTGAGATTATGGGGAAGGTTACTTATATGGGTCCGGCTGGGTCCGGGCAGAGTTGTAAAATTGCTAACCAGATCACTGTGGGGGCCAATTTGCTTGGATTGAGTGAAGGGTTGGTGTTTGCGGAGCGGGCTGGGTTGGATGTAAAGCGGTTTATGGAGGCGGTGAGGGGCGGGGCGGCGGGGTCTAAAGTAATGGAGTTGTTTGGGGAGAGGATGATTGAGAGGGACTTCAGGCCTGGTGGGTTTGCAGAGTATATGGTGAAGGATATGGGGATGGGTGTGGATGTTGTGGAGGAAGGTGAGGATGGGAGAGTGGTAGTGTTGCCTGGTGCTGCATTGTGCAAGCAGTTGTTTTCGGGTATGGTGGCTAATGGTGACGGTAAGCTTGGTACGCAAGGGCTTATCACTGCTATAGAGAGGCTCAATGGAAACATGTTTCATATAAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

313

Amino Acids

32.59

Weight (kDa)

6.53

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 4.3e-45 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 77 2.5e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 298 9.8e-27 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccB1I GGYRCC 3 cut(s) 167, 416, 478
AccBSI CCGCTC 2 cut(s) 149, 613
AccI GTMKAC 3 cut(s) 123, 312, 387
AccII CGCG 4 cut(s) 341, 343, 366, 392
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 700
AcyI GRCGYC 4 cut(s) 168, 213, 417, 479
AfaI GTAC 1 cut(s) 877
AfiI CCNNNNNNNGG 2 cut(s) 163, 401
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 2 cut(s) 141, 194
AjiI CACGTC 1 cut(s) 471
AjnI CCWGG 4 cut(s) 162, 419, 721, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 3 cut(s) 104, 433, 871
AluI AGCT 3 cut(s) 104, 433, 871
AlwI GGATC 1 cut(s) 246
AoxI GGCC 5 cut(s) 33, 84, 232, 570, 719
ApeKI GCWGC 3 cut(s) 77, 324, 818
AspLEI GCGC 8 cut(s) 77, 170, 337, 343, 368, 394, 419, 481
AspS9I GGNCC 4 cut(s) 232, 518, 528, 570
AsuC2I CCSGG 2 cut(s) 332, 532
AsuHPI GGTGA 8 cut(s) 109, 214, 298, 478, 661, 757, 797, 872
AvaII GGWCC 2 cut(s) 518, 528
BanI GGYRCC 3 cut(s) 167, 416, 478
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 3 cut(s) 64, 336, 805
BccI CCATC 2 cut(s) 754, 785
BciT130I CCWGG 4 cut(s) 164, 421, 723, 813
BciVI GTATCC 1 cut(s) 409
BcnI CCSGG 2 cut(s) 332, 532
BfmI CTRYAG 1 cut(s) 897
BfoI RGCGCY 5 cut(s) 78, 171, 338, 420, 482
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 7 cut(s) 78, 147, 325, 357, 406, 663, 819
BlsI GCNGC 7 cut(s) 79, 148, 326, 358, 407, 664, 820
Bme1390I CCNGG 6 cut(s) 164, 332, 421, 532, 723, 813
Bme18I GGWCC 2 cut(s) 518, 528
BmgBI CACGTC 1 cut(s) 471
BmgT120I GGNCC 4 cut(s) 232, 518, 528, 570
BmiI GGNNCC 6 cut(s) 169, 418, 480, 519, 529, 571
BmrFI CCNGG 6 cut(s) 164, 332, 421, 532, 723, 813
BmsI GCATC 3 cut(s) 54, 69, 445
BpiI GAAGAC 1 cut(s) 211
BpuMI CCSGG 2 cut(s) 332, 532
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 4 cut(s) 168, 213, 417, 479
BsaJI CCNNGG 6 cut(s) 81, 162, 163, 225, 420, 459
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 401
Bse118I RCCGGY 2 cut(s) 394, 446
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 4 cut(s) 164, 421, 723, 813
BseDI CCNNGG 6 cut(s) 81, 162, 163, 225, 420, 459
BseGI GGATG 6 cut(s) 460, 631, 705, 765, 775, 796
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 2 cut(s) 163, 401
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 3 cut(s) 64, 336, 805
BseYI CCCAGC 2 cut(s) 524, 617
Bsh1236I CGCG 4 cut(s) 341, 343, 366, 392
BshFI GGCC 5 cut(s) 35, 86, 234, 572, 721
BshNI GGYRCC 3 cut(s) 167, 416, 478
BsiSI CCGG 7 cut(s) 32, 332, 395, 402, 447, 521, 531
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 2 cut(s) 163, 401
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 5 cut(s) 35, 86, 234, 572, 721
Bsp143I GATC 2 cut(s) 251, 558
Bsp19I CCATGG 2 cut(s) 81, 225
BspANI GGCC 5 cut(s) 35, 86, 234, 572, 721
BspCNI CTCAG 1 cut(s) 113
BspFNI CGCG 4 cut(s) 341, 343, 366, 392
BspLI GGNNCC 6 cut(s) 169, 418, 480, 519, 529, 571
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 3 cut(s) 167, 416, 478
BsrBI CCGCTC 2 cut(s) 149, 613
BsrFI RCCGGY 2 cut(s) 394, 446
BssAI RCCGGY 2 cut(s) 394, 446
BssECI CCNNGG 6 cut(s) 81, 162, 163, 225, 420, 459
BssMI GATC 2 cut(s) 251, 558
BssNI GRCGYC 4 cut(s) 168, 213, 417, 479
BssT1I CCWWGG 2 cut(s) 81, 225
Bst2UI CCWGG 4 cut(s) 164, 421, 723, 813
Bst4CI ACNGT 4 cut(s) 121, 310, 565, 866
BstACI GRCGYC 4 cut(s) 168, 213, 417, 479
BstC8I GCNNGC 9 cut(s) 106, 130, 339, 431, 435, 448, 477, 615, 830
BstDEI CTNAG 1 cut(s) 100
BstDSI CCRYGG 2 cut(s) 81, 225
BstF5I GGATG 6 cut(s) 460, 631, 705, 765, 775, 796
BstFNI CGCG 4 cut(s) 341, 343, 366, 392
BstH2I RGCGCY 5 cut(s) 78, 171, 338, 420, 482
BstHHI GCGC 8 cut(s) 77, 170, 337, 343, 368, 394, 419, 481
BstKTI GATC 2 cut(s) 254, 561
BstMBI GATC 2 cut(s) 251, 558
BstMWI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 414, 443, 614, 877
BstNI CCWGG 4 cut(s) 164, 421, 723, 813
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 6 cut(s) 162, 330, 419, 530, 721, 811
BstSFI CTRYAG 1 cut(s) 897
BstUI CGCG 4 cut(s) 341, 343, 366, 392
BstV1I GCAGC 3 cut(s) 64, 336, 805
BstV2I GAAGAC 1 cut(s) 211
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 5 cut(s) 35, 86, 234, 572, 721
BtgI CCRYGG 2 cut(s) 81, 225
BtrI CACGTC 1 cut(s) 471
BtsCI GGATG 6 cut(s) 460, 631, 705, 765, 775, 796
BtsI GCAGTG 2 cut(s) 373, 891
BtsIMutI CAGTG 3 cut(s) 373, 561, 891
Cac8I GCNNGC 9 cut(s) 106, 130, 339, 431, 435, 448, 477, 615, 830
CfoI GCGC 8 cut(s) 77, 170, 337, 343, 368, 394, 419, 481
Cfr10I RCCGGY 2 cut(s) 394, 446
Cfr13I GGNCC 4 cut(s) 232, 518, 528, 570
CseI GACGC 1 cut(s) 398
Csp6I GTAC 1 cut(s) 876
CviAII CATG 4 cut(s) 82, 226, 316, 919
CviQI GTAC 1 cut(s) 876
DdeI CTNAG 1 cut(s) 100
DinI GGCGCC 3 cut(s) 169, 418, 480
DpnI GATC 2 cut(s) 253, 560
DpnII GATC 2 cut(s) 251, 558
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 81, 225
Eco147I AGGCCT 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 518, 528
Eco57I CTGAAG 1 cut(s) 700
EcoRII CCWGG 4 cut(s) 162, 419, 721, 811
EcoT14I CCWWGG 2 cut(s) 81, 225
EgeI GGCGCC 3 cut(s) 169, 418, 480
EheI GGCGCC 3 cut(s) 169, 418, 480
ErhI CCWWGG 2 cut(s) 81, 225
FaeI CATG 4 cut(s) 85, 229, 319, 922
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 725
FatI CATG 4 cut(s) 81, 225, 315, 918
FauI CCCGC 6 cut(s) 50, 98, 443, 606, 650, 658
FblI GTMKAC 3 cut(s) 123, 312, 387
Fnu4HI GCNGC 7 cut(s) 78, 147, 325, 357, 406, 663, 819
FokI GGATG 6 cut(s) 467, 638, 712, 772, 782, 803
Fsp4HI GCNGC 7 cut(s) 78, 147, 325, 357, 406, 663, 819
GlaI GCGC 8 cut(s) 76, 169, 336, 342, 367, 393, 418, 480
GluI GCNGC 7 cut(s) 78, 147, 325, 357, 406, 663, 819
GsaI CCCAGC 2 cut(s) 528, 621
HaeII RGCGCY 5 cut(s) 78, 171, 338, 420, 482
HaeIII GGCC 5 cut(s) 35, 86, 234, 572, 721
HapII CCGG 7 cut(s) 32, 332, 395, 402, 447, 521, 531
HgaI GACGC 1 cut(s) 398
HhaI GCGC 8 cut(s) 77, 170, 337, 343, 368, 394, 419, 481
Hin1I GRCGYC 4 cut(s) 168, 213, 417, 479
Hin1II CATG 4 cut(s) 85, 229, 319, 922
Hin6I GCGC 8 cut(s) 75, 168, 335, 341, 366, 392, 417, 479
HinP1I GCGC 8 cut(s) 75, 168, 335, 341, 366, 392, 417, 479
HincII GTYRAC 2 cut(s) 313, 388
HindII GTYRAC 2 cut(s) 313, 388
HindIII AAGCTT 2 cut(s) 431, 869
HinfI GANTC 1 cut(s) 182
HpaII CCGG 7 cut(s) 32, 332, 395, 402, 447, 521, 531
HphI GGTGA 8 cut(s) 109, 214, 298, 478, 661, 757, 797, 872
Hpy166II GTNNAC 3 cut(s) 124, 313, 388
Hpy188I TCNGA 3 cut(s) 211, 244, 251
Hpy8I GTNNAC 3 cut(s) 124, 313, 388
Hpy99I CGWCG 4 cut(s) 215, 218, 314, 392
HpyAV CCTTC 7 cut(s) 147, 287, 419, 496, 587, 742, 776
HpyCH4III ACNGT 4 cut(s) 121, 310, 565, 866
HpyCH4IV ACGT 2 cut(s) 213, 470
HpyCH4V TGCA 3 cut(s) 734, 821, 828
HpyF10VI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 414, 443, 614, 877
HpyF3I CTNAG 1 cut(s) 100
HpySE526I ACGT 2 cut(s) 213, 470
Hsp92I GRCGYC 4 cut(s) 168, 213, 417, 479
Hsp92II CATG 4 cut(s) 85, 229, 319, 922
HspAI GCGC 8 cut(s) 75, 168, 335, 341, 366, 392, 417, 479
KasI GGCGCC 3 cut(s) 167, 416, 478
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
Kzo9I GATC 2 cut(s) 251, 558
LmnI GCTCC 2 cut(s) 154, 610
Lsp1109I GCAGC 3 cut(s) 64, 336, 805
LweI GCATC 3 cut(s) 54, 69, 445
MaeII ACGT 2 cut(s) 213, 470
MaeIII GTNAC 4 cut(s) 304, 466, 505, 860
MalI GATC 2 cut(s) 253, 560
MbiI CCGCTC 2 cut(s) 149, 613
MboI GATC 2 cut(s) 251, 558
MboII GAAGA 1 cut(s) 211
MluCI AATT 3 cut(s) 298, 546, 574
Mly113I GGCGCC 3 cut(s) 168, 417, 479
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 3 cut(s) 234, 279, 603
MreI CGCCGGCG 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 7 cut(s) 32, 332, 395, 402, 447, 521, 531
MspR9I CCNGG 6 cut(s) 164, 332, 421, 532, 723, 813
MvaI CCWGG 4 cut(s) 164, 421, 723, 813
MvnI CGCG 4 cut(s) 341, 343, 366, 392
MwoI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 414, 443, 614, 877
NaeI GCCGGC 1 cut(s) 448
NarI GGCGCC 3 cut(s) 168, 417, 479
NciI CCSGG 2 cut(s) 332, 532
NcoI CCATGG 2 cut(s) 81, 225
NdeII GATC 2 cut(s) 251, 558
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 4 cut(s) 85, 229, 319, 922
NlaIV GGNNCC 6 cut(s) 169, 418, 480, 519, 529, 571
NmuCI GTSAC 3 cut(s) 304, 466, 860
NspI RCATGY 1 cut(s) 922
OliI CACNNNNGTG 1 cut(s) 227
PasI CCCWGGG 1 cut(s) 163
PceI AGGCCT 1 cut(s) 721
PciI ACATGT 1 cut(s) 918
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PdiI GCCGGC 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 7 cut(s) 79, 148, 326, 358, 407, 664, 820
PleI GAGTC 1 cut(s) 176
PluTI GGCGCC 3 cut(s) 171, 420, 482
PpsI GAGTC 1 cut(s) 176
PscI ACATGT 1 cut(s) 918
Psp6I CCWGG 4 cut(s) 162, 419, 721, 811
PspFI CCCAGC 2 cut(s) 524, 617
PspGI CCWGG 4 cut(s) 162, 419, 721, 811
PspN4I GGNNCC 6 cut(s) 169, 418, 480, 519, 529, 571
PspPI GGNCC 4 cut(s) 232, 518, 528, 570
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 877
RsaNI GTAC 1 cut(s) 876
RseI CAYNNNNRTG 1 cut(s) 227
SalI GTCGAC 2 cut(s) 311, 386
SatI GCNGC 7 cut(s) 78, 147, 325, 357, 406, 663, 819
Sau3AI GATC 2 cut(s) 251, 558
Sau96I GGNCC 4 cut(s) 232, 518, 528, 570
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 6 cut(s) 164, 332, 421, 532, 723, 813
SetI ASST 9 cut(s) 106, 177, 216, 264, 435, 473, 507, 787, 873
SfaNI GCATC 3 cut(s) 54, 69, 445
SfcI CTRYAG 1 cut(s) 897
SfoI GGCGCC 3 cut(s) 169, 418, 480
SgrAI CRCCGGYG 1 cut(s) 446
SinI GGWCC 2 cut(s) 518, 528
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 3 cut(s) 298, 546, 574
SseBI AGGCCT 1 cut(s) 721
SspDI GGCGCC 3 cut(s) 167, 416, 478
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 6 cut(s) 162, 330, 419, 530, 721, 811
StyI CCWWGG 2 cut(s) 81, 225
TaaI ACNGT 4 cut(s) 121, 310, 565, 866
TaiI ACGT 2 cut(s) 216, 473
TaqI TCGA 2 cut(s) 312, 387
TasI AATT 3 cut(s) 298, 546, 574
TauI GCSGC 4 cut(s) 149, 359, 408, 665
TscAI CASTG 3 cut(s) 380, 568, 898
TseFI GTSAC 3 cut(s) 304, 466, 860
TseI GCWGC 3 cut(s) 77, 324, 818
Tsp45I GTSAC 3 cut(s) 304, 466, 860
TspDTI ATGAA 1 cut(s) 914
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 3 cut(s) 380, 568, 898
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 2 cut(s) 518, 528
XceI RCATGY 1 cut(s) 922
XmiI GTMKAC 3 cut(s) 123, 312, 387
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.