MD15G1425100.v1.1

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004641
Physical Location & Seq
Reverse (-)
52614383 .. 52615939
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1425100.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGGAACCCCTTACCCGAACCCCATCACTCCTGCCCAAACCCAGATCGGGTGGATCGGTATTGGCTTAATGGGTGCAGCCATGGCCTCCCGCCTCCTCTCCGCCGGCTACTCCCTCACCATCTACGCTCGCACTCCGTCCAAAGCCCTCTCCCTCCAATCCCAGGGTGCCCGCCTCGCCAAATCCCCGTTTGAAGTTGCTAAATCCTGCGACGTTGTTTTCACCATTGTGGGACACCCGCCGGATGTTCGACAAGTTATCTTGGAGACAAACGGCGTCCTTTCGGGCCTAAACCCTAACGGCGTCACGATATCTGCTGCCGCGCGAGCCAGAGGTTGCTGGGCTGTGGATGCCCCAGTTTCTGGGGCCGACGTGGGTGCTAGGGAAGGAAAACTTGGAATATTCGCCGGCGGAGATGCTGGAGTTGTGAAGTGGTTGACGCCGTTGTTTGACATCATGGGAAAGGTTACCTACATGGGGGAAGCCGGGTGCGGTCAGAGTTGCAAAATAGGAAACAACATTGTGGGCGGACTGGGGTTGGTTGGGTTGAGTGAAGGGCTGGTGTTTGCGGAGCGGGCGGGGTTGGATTTAAAGCAGTTTTTGGAGGCGGTGAGAGGAGGGGCGGCGGGGTCAACGCTGATGGAGTTGTTTGGGGAGAAAATGATAGGGAGGGACATGAGGCCTGGCGGGTTTGCAGAGTATTTGGTGAAGGATTTGGGGATGGGAGTGGATGTGGTGGAGGAGGAGGAGGATGAGAGGGTGGTGGTGCTGCCGGGGGCGGCGTTAAGTAAGCAGATGTATTCAGCGATGGTGGCTAATGGGGATGGGAAGCTTGGCATTCATGGGGTTATCTCTCTGCGGCGACGATCTTTGTGGGGCTTGTTGGAGGACTACGGAGCCTTGTTCTTCAATTGTGGGGCTTTGTTTGGTGTGATCTCAGGAGTCTGCAAAGGGTGTTGGTTTGCAATGGGTGGTCCTTCAATCCTGGGCTTTGTTTGGTTTGATCTCAGGCGTTTGCAGAGGGTGTTGGTTTGCAATGGGTGGTTGGAGCTCCGGCAGCGGCAATATTATGCTAGGGTTTCTTTGTTTAGGTCTTTCTGTTGGCTCCAGACCTTGTGTTGGCTCTTTTTTGTGTTGCTTTTGGGGGTCCATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

386

Amino Acids

41.13

Weight (kDa)

8.49

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 160 1.5e-34 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 90 7e-08 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 163 - 284 1.3e-21 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 574
AccII CGCG 2 cut(s) 323, 325
AclWI GGATC 1 cut(s) 62
AcyI GRCGYC 3 cut(s) 276, 303, 440
AfiI CCNNNNNNNGG 5 cut(s) 48, 163, 362, 477, 1119
AgsI TTSAA 3 cut(s) 194, 910, 981
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 3 cut(s) 162, 682, 984
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 2 cut(s) 832, 1051
AluI AGCT 2 cut(s) 832, 1051
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 4 cut(s) 84, 286, 366, 680
ApeKI GCWGC 4 cut(s) 77, 317, 769, 1057
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 4 cut(s) 286, 366, 974, 1147
AsuC2I CCSGG 2 cut(s) 487, 774
AsuHPI GGTGA 4 cut(s) 109, 214, 622, 718
AvaII GGWCC 2 cut(s) 974, 1147
BaeGI GKGCMC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 1053
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 4 cut(s) 89, 304, 756, 1069
BccI CCATC 6 cut(s) 32, 128, 634, 715, 802, 818
BceAI ACGGC 3 cut(s) 289, 316, 427
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 3 cut(s) 164, 684, 986
BcnI CCSGG 2 cut(s) 487, 774
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 2 cut(s) 381, 1074
BisI GCNGC 9 cut(s) 78, 318, 321, 624, 770, 780, 860, 1058, 1061
BlsI GCNGC 9 cut(s) 79, 319, 322, 625, 771, 781, 861, 1059, 1062
Bme1390I CCNGG 5 cut(s) 164, 487, 684, 774, 986
Bme18I GGWCC 2 cut(s) 974, 1147
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 4 cut(s) 286, 366, 974, 1147
BmiI GGNNCC 6 cut(s) 7, 169, 367, 898, 1106, 1148
BmrFI CCNGG 5 cut(s) 164, 487, 684, 774, 986
BmrI ACTGGG 2 cut(s) 350, 542
BmsI GCATC 2 cut(s) 340, 406
BmuI ACTGGG 2 cut(s) 350, 542
BpmI CTGGAG 2 cut(s) 441, 1091
BpuMI CCSGG 2 cut(s) 487, 774
BsaHI GRCGYC 3 cut(s) 276, 303, 440
BsaJI CCNNGG 5 cut(s) 81, 162, 163, 773, 985
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 163, 362, 477, 1119
Bse118I RCCGGY 2 cut(s) 104, 407
Bse1I ACTGG 2 cut(s) 356, 537
Bse3DI GCAATG 2 cut(s) 972, 1042
BseBI CCWGG 3 cut(s) 164, 684, 986
BseDI CCNNGG 5 cut(s) 81, 162, 163, 773, 985
BseGI GGATG 6 cut(s) 250, 355, 726, 736, 757, 829
BseLI CCNNNNNNNGG 5 cut(s) 48, 163, 362, 477, 1119
BseMI GCAATG 2 cut(s) 972, 1042
BseMII CTCAG 2 cut(s) 951, 1021
BseNI ACTGG 2 cut(s) 356, 537
BseRI GAGGAG 5 cut(s) 86, 630, 755, 758, 761
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 4 cut(s) 89, 304, 756, 1069
BseYI CCCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 323, 325
BshFI GGCC 4 cut(s) 86, 288, 368, 682
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 1053
BsiSI CCGG 6 cut(s) 105, 242, 408, 486, 773, 1054
BslFI GGGAC 2 cut(s) 246, 686
BslI CCNNNNNNNGG 5 cut(s) 48, 163, 362, 477, 1119
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 246, 686
BsmI GAATGC 1 cut(s) 837
BsnI GGCC 4 cut(s) 86, 288, 368, 682
Bsp1286I GDGCHC 2 cut(s) 172, 1053
Bsp143I GATC 5 cut(s) 45, 54, 866, 933, 1003
Bsp19I CCATGG 1 cut(s) 81
BspANI GGCC 4 cut(s) 86, 288, 368, 682
BspCNI CTCAG 2 cut(s) 950, 1020
BspFNI CGCG 2 cut(s) 323, 325
BspLI GGNNCC 6 cut(s) 7, 169, 367, 898, 1106, 1148
BspPI GGATC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 574
BsrDI GCAATG 2 cut(s) 972, 1042
BsrFI RCCGGY 2 cut(s) 104, 407
BsrI ACTGG 2 cut(s) 356, 537
BssAI RCCGGY 2 cut(s) 104, 407
BssECI CCNNGG 5 cut(s) 81, 162, 163, 773, 985
BssMI GATC 5 cut(s) 45, 54, 866, 933, 1003
BssNI GRCGYC 3 cut(s) 276, 303, 440
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 3 cut(s) 164, 684, 986
BstACI GRCGYC 3 cut(s) 276, 303, 440
BstC8I GCNNGC 6 cut(s) 106, 130, 172, 327, 409, 576
BstDEI CTNAG 2 cut(s) 937, 1007
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 6 cut(s) 250, 355, 726, 736, 757, 829
BstFNI CGCG 2 cut(s) 323, 325
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 5 cut(s) 48, 57, 869, 936, 1006
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 5 cut(s) 45, 54, 866, 933, 1003
BstMWI GCNNNNNNNGC 7 cut(s) 83, 176, 326, 350, 575, 812, 1057
BstNI CCWGG 3 cut(s) 164, 684, 986
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 5 cut(s) 162, 485, 682, 772, 984
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 2 cut(s) 323, 325
BstV1I GCAGC 4 cut(s) 89, 304, 756, 1069
BsuRI GGCC 4 cut(s) 86, 288, 368, 682
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 821
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 6 cut(s) 250, 355, 726, 736, 757, 829
Cac8I GCNNGC 6 cut(s) 106, 130, 172, 327, 409, 576
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 325
Cfr10I RCCGGY 2 cut(s) 104, 407
Cfr13I GGNCC 4 cut(s) 286, 366, 974, 1147
CseI GACGC 3 cut(s) 265, 292, 448
CviAII CATG 5 cut(s) 82, 457, 475, 676, 842
DdeI CTNAG 2 cut(s) 937, 1007
DpnI GATC 5 cut(s) 47, 56, 868, 935, 1005
DpnII GATC 5 cut(s) 45, 54, 866, 933, 1003
DraI TTTAAA 1 cut(s) 591
EciI GGCGGA 3 cut(s) 91, 426, 543
Ecl136II GAGCTC 1 cut(s) 1051
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 682
Eco24I GRGCYC 1 cut(s) 1053
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 974, 1147
Eco53kI GAGCTC 1 cut(s) 1051
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 1051
EcoO65I GGTNACC 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 162, 682, 984
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 1053
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 85, 460, 478, 679, 845
FaiI YATR 7 cut(s) 83, 458, 476, 677, 843, 1071, 1152
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 246, 686
FatI CATG 5 cut(s) 81, 456, 474, 675, 841
FauI CCCGC 7 cut(s) 98, 179, 246, 567, 571, 619, 680
Fnu4HI GCNGC 9 cut(s) 78, 318, 321, 624, 770, 780, 860, 1058, 1061
FokI GGATG 6 cut(s) 257, 362, 733, 743, 764, 836
FriOI GRGCYC 1 cut(s) 1053
Fsp4HI GCNGC 9 cut(s) 78, 318, 321, 624, 770, 780, 860, 1058, 1061
FspBI CTAG 2 cut(s) 381, 1074
GlaI GCGC 1 cut(s) 324
GluI GCNGC 9 cut(s) 78, 318, 321, 624, 770, 780, 860, 1058, 1061
GsaI CCCAGC 1 cut(s) 343
GsuI CTGGAG 2 cut(s) 441, 1091
HaeIII GGCC 4 cut(s) 86, 288, 368, 682
HapII CCGG 6 cut(s) 105, 242, 408, 486, 773, 1054
HgaI GACGC 3 cut(s) 265, 292, 448
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 3 cut(s) 276, 303, 440
Hin1II CATG 5 cut(s) 85, 460, 478, 679, 845
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 2 cut(s) 438, 633
HindII GTYRAC 2 cut(s) 438, 633
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 1 cut(s) 942
HpaII CCGG 6 cut(s) 105, 242, 408, 486, 773, 1054
HphI GGTGA 4 cut(s) 109, 214, 622, 718
Hpy166II GTNNAC 2 cut(s) 438, 633
Hpy188I TCNGA 1 cut(s) 498
Hpy188III TCNNGA 3 cut(s) 307, 939, 1108
Hpy8I GTNNAC 2 cut(s) 438, 633
Hpy99I CGWCG 3 cut(s) 215, 374, 867
HpyAV CCTTC 4 cut(s) 380, 548, 703, 987
HpyCH4IV ACGT 2 cut(s) 213, 372
HpyCH4V TGCA 7 cut(s) 77, 504, 695, 948, 965, 1018, 1035
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 176, 326, 350, 575, 812, 1057
HpyF3I CTNAG 2 cut(s) 937, 1007
HpySE526I ACGT 2 cut(s) 213, 372
Hsp92I GRCGYC 3 cut(s) 276, 303, 440
Hsp92II CATG 5 cut(s) 85, 460, 478, 679, 845
HspAI GCGC 1 cut(s) 323
KroI GCCGGC 2 cut(s) 104, 407
KroNI GCCGGC 2 cut(s) 106, 409
Kzo9I GATC 5 cut(s) 45, 54, 866, 933, 1003
LmnI GCTCC 5 cut(s) 571, 896, 1048, 1056, 1110
Lsp1109I GCAGC 4 cut(s) 89, 304, 756, 1069
LweI GCATC 2 cut(s) 340, 406
MaeI CTAG 2 cut(s) 381, 1074
MaeII ACGT 2 cut(s) 213, 372
MaeIII GTNAC 2 cut(s) 304, 466
MalI GATC 5 cut(s) 47, 56, 868, 935, 1005
MbiI CCGCTC 1 cut(s) 574
MboI GATC 5 cut(s) 45, 54, 866, 933, 1003
MboII GAAGA 1 cut(s) 898
MfeI CAATTG 1 cut(s) 910
MhlI GDGCHC 2 cut(s) 172, 1053
MluCI AATT 1 cut(s) 910
MlyI GAGTC 1 cut(s) 951
MmeI TCCRAC 3 cut(s) 564, 864, 1026
MreI CGCCGGCG 1 cut(s) 407
MroNI GCCGGC 2 cut(s) 104, 407
MseI TTAA 3 cut(s) 68, 590, 785
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 1060
MspI CCGG 6 cut(s) 105, 242, 408, 486, 773, 1054
MspR9I CCNGG 5 cut(s) 164, 487, 684, 774, 986
MunI CAATTG 1 cut(s) 910
Mva1269I GAATGC 1 cut(s) 837
MvaI CCWGG 3 cut(s) 164, 684, 986
MvnI CGCG 2 cut(s) 323, 325
MwoI GCNNNNNNNGC 7 cut(s) 83, 176, 326, 350, 575, 812, 1057
NaeI GCCGGC 2 cut(s) 106, 409
NciI CCSGG 2 cut(s) 487, 774
NcoI CCATGG 1 cut(s) 81
NdeII GATC 5 cut(s) 45, 54, 866, 933, 1003
NgoMIV GCCGGC 2 cut(s) 104, 407
NlaIII CATG 5 cut(s) 85, 460, 478, 679, 845
NlaIV GGNNCC 6 cut(s) 7, 169, 367, 898, 1106, 1148
NmuCI GTSAC 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 227
PasI CCCWGGG 1 cut(s) 163
PceI AGGCCT 1 cut(s) 682
PctI GAATGC 1 cut(s) 837
PdiI GCCGGC 2 cut(s) 106, 409
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 9 cut(s) 79, 319, 322, 625, 771, 781, 861, 1059, 1062
PleI GAGTC 1 cut(s) 950
PpsI GAGTC 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 1053
Psp6I CCWGG 3 cut(s) 162, 682, 984
PspEI GGTNACC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 339
PspGI CCWGG 3 cut(s) 162, 682, 984
PspN4I GGNNCC 6 cut(s) 7, 169, 367, 898, 1106, 1148
PspPI GGNCC 4 cut(s) 286, 366, 974, 1147
PstNI CAGNNNCTG 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 1053
SaqAI TTAA 3 cut(s) 68, 590, 785
SatI GCNGC 9 cut(s) 78, 318, 321, 624, 770, 780, 860, 1058, 1061
Sau3AI GATC 5 cut(s) 45, 54, 866, 933, 1003
Sau96I GGNCC 4 cut(s) 286, 366, 974, 1147
SchI GAGTC 1 cut(s) 951
ScrFI CCNGG 5 cut(s) 164, 487, 684, 774, 986
SduI GDGCHC 2 cut(s) 172, 1053
SetI ASST 9 cut(s) 216, 337, 375, 468, 473, 834, 1053, 1094, 1115
SfaNI GCATC 2 cut(s) 340, 406
SgrAI CRCCGGYG 1 cut(s) 407
SinI GGWCC 2 cut(s) 974, 1147
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 1 cut(s) 910
SseBI AGGCCT 1 cut(s) 682
SspI AATATT 2 cut(s) 402, 1067
SspMI CTAG 2 cut(s) 381, 1074
SstI GAGCTC 1 cut(s) 1053
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 5 cut(s) 162, 485, 682, 772, 984
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 216, 375
TaqI TCGA 1 cut(s) 250
TasI AATT 1 cut(s) 910
TauI GCSGC 5 cut(s) 323, 626, 782, 862, 1063
Tru1I TTAA 3 cut(s) 68, 590, 785
Tru9I TTAA 3 cut(s) 68, 590, 785
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 77, 317, 769, 1057
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 830
TspGWI ACGGA 2 cut(s) 126, 909
Van91I CCANNNNNTGG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 974, 1147
XspI CTAG 2 cut(s) 381, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.