pycom15g37680

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004641
Physical Location & Seq
Reverse (-)
37611482 .. 37612414
933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g37680.1

Sequence Viewer

Length: 933 bp
ATGGAAACCCCTTACCCGAACCCCATCACTCCTGCCCAAACCCAGATCGGGTGGATCGGTATTGGCTTAATGGGTGCAGCCATGGCCTCCCGCCTCCTCTCCGCCGGCTACTCCCTCACCATCTACGCTCGCACTCCGTCCAAAGCCCTCTCCCTCCAATCCCAGGGCGCCCGCCTCCCCGAATCCCCGTTTGAAGTTGCAAAATCCTGCGAAGTTGTTTTCACCATTGTGGGACACCCGCCGGATGTTCGACAAGTTATCTTGGAGACAAACGGCGTCCTTTCGGGCCTAAACCCGAACGGTGTCACGGTGGATATGACTACCAATCTACCCTCCATGGCACGAGAGATATCTGCTGCCGCGCGCGCCCGAGATTGCTGGGTTGTGGATGCCCCAGTTTCTGGGGCGGACGTGGGTGCTAGGGAGGGAAAACTTGGAATATTTGCCGGCGGAGATGCCGGAGTTGTGAAGTGGTTGACGCCGTTGTTTGACATCATGGGAAATGTTACCTACATGGGGGAAGCCGGGTGTGGTCAGAGTTGCAAAATAGGAAACAACATCGTGGGCGGAGTGGGGTTGGTTGGGTTGAGTGAAGGGCTGGTGTTTGCGGAGCGGGCGGGGTTGGATTTGAAGCAGTTTTTGGAGGCGATGAGAGGAGGGGCGGCGGGGTCAACGCTGATGGAGTTGTTTGGGGAGAAAATGATAGAGAGGGACATGAGGCCTGGCGGGTTTGCAGAGTATTTGGTGAAGGATTTGGGGATGGGAGTGGATGTGGTGGAGGAGGAGGAGGAGGATAGGAGGGTGGTGGTGCTGCCGGGGGCGGCGTTAAGTAAGCAGATGTATTCAGCGATGGTGGCTAATGGGGATGGGAAGCTTGGCATTCATGGGGTTATCTCTGTTATTGACAGGCTCAATGGCAACATTAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

311

Amino Acids

32.5

Weight (kDa)

4.97

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 1.4e-43 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 89 3.6e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 300 8.7e-22 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 401
AccBSI CCGCTC 1 cut(s) 613
AccII CGCG 3 cut(s) 362, 364, 366
AclWI GGATC 1 cut(s) 62
AcyI GRCGYC 3 cut(s) 168, 276, 479
AfiI CCNNNNNNNGG 4 cut(s) 48, 163, 401, 516
AgsI TTSAA 2 cut(s) 194, 631
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 2 cut(s) 162, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 1 cut(s) 874
AluI AGCT 1 cut(s) 874
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 369
AoxI GGCC 3 cut(s) 84, 286, 719
ApeKI GCWGC 3 cut(s) 77, 356, 811
AspLEI GCGC 4 cut(s) 170, 364, 366, 368
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 2 cut(s) 526, 816
AsuHPI GGTGA 3 cut(s) 109, 214, 757
AvaI CYCGRG 1 cut(s) 369
BanI GGYRCC 1 cut(s) 167
BauI CACGAG 1 cut(s) 342
BbvI GCAGC 3 cut(s) 89, 343, 798
BccI CCATC 6 cut(s) 32, 128, 673, 754, 844, 860
BceAI ACGGC 2 cut(s) 289, 466
BciT130I CCWGG 2 cut(s) 164, 723
BcnI CCSGG 2 cut(s) 526, 816
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 6 cut(s) 78, 357, 360, 663, 812, 822
BlsI GCNGC 6 cut(s) 79, 358, 361, 664, 813, 823
Bme1390I CCNGG 4 cut(s) 164, 526, 723, 816
BmeT110I CYCGRG 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 4 cut(s) 164, 526, 723, 816
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 379, 445
BmuI ACTGGG 1 cut(s) 389
BpuMI CCSGG 2 cut(s) 526, 816
BsaHI GRCGYC 3 cut(s) 168, 276, 479
BsaJI CCNNGG 5 cut(s) 81, 162, 163, 336, 815
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 163, 401, 516
Bse118I RCCGGY 2 cut(s) 104, 446
Bse1I ACTGG 1 cut(s) 395
BseBI CCWGG 2 cut(s) 164, 723
BseDI CCNNGG 5 cut(s) 81, 162, 163, 336, 815
BseGI GGATG 5 cut(s) 250, 394, 765, 775, 871
BseLI CCNNNNNNNGG 4 cut(s) 48, 163, 401, 516
BseNI ACTGG 1 cut(s) 395
BsePI GCGCGC 2 cut(s) 362, 364
BseRI GAGGAG 6 cut(s) 86, 669, 794, 797, 800, 803
BseXI GCAGC 3 cut(s) 89, 343, 798
BseYI CCCAGC 1 cut(s) 378
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 3 cut(s) 362, 364, 366
BshFI GGCC 3 cut(s) 86, 288, 721
BshNI GGYRCC 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 6 cut(s) 105, 242, 447, 459, 525, 815
BslFI GGGAC 2 cut(s) 246, 725
BslI CCNNNNNNNGG 4 cut(s) 48, 163, 401, 516
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 246, 725
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 3 cut(s) 86, 288, 721
BsoBI CYCGRG 1 cut(s) 369
Bsp143I GATC 2 cut(s) 45, 54
Bsp19I CCATGG 2 cut(s) 81, 336
BspANI GGCC 3 cut(s) 86, 288, 721
BspFNI CGCG 3 cut(s) 362, 364, 366
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 613
BsrFI RCCGGY 2 cut(s) 104, 446
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 2 cut(s) 104, 446
BssECI CCNNGG 5 cut(s) 81, 162, 163, 336, 815
BssHII GCGCGC 2 cut(s) 362, 364
BssMI GATC 2 cut(s) 45, 54
BssNI GRCGYC 3 cut(s) 168, 276, 479
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 2 cut(s) 81, 336
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 164, 723
Bst4CI ACNGT 2 cut(s) 302, 310
BstACI GRCGYC 3 cut(s) 168, 276, 479
BstC8I GCNNGC 7 cut(s) 106, 130, 172, 364, 366, 448, 615
BstDSI CCRYGG 2 cut(s) 81, 336
BstF5I GGATG 5 cut(s) 250, 394, 765, 775, 871
BstFNI CGCG 3 cut(s) 362, 364, 366
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 4 cut(s) 170, 364, 366, 368
BstKTI GATC 2 cut(s) 48, 57
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 45, 54
BstMWI GCNNNNNNNGC 5 cut(s) 83, 365, 614, 854, 924
BstNI CCWGG 2 cut(s) 164, 723
BstSCI CCNGG 4 cut(s) 162, 524, 721, 814
BstUI CGCG 3 cut(s) 362, 364, 366
BstV1I GCAGC 3 cut(s) 89, 343, 798
BsuRI GGCC 3 cut(s) 86, 288, 721
BtgI CCRYGG 2 cut(s) 81, 336
BtgZI GCGATG 2 cut(s) 662, 863
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 5 cut(s) 250, 394, 765, 775, 871
Cac8I GCNNGC 7 cut(s) 106, 130, 172, 364, 366, 448, 615
CaiI CAGNNNCTG 1 cut(s) 401
CfoI GCGC 4 cut(s) 170, 364, 366, 368
Cfr10I RCCGGY 2 cut(s) 104, 446
Cfr13I GGNCC 1 cut(s) 286
CseI GACGC 2 cut(s) 265, 487
CviAII CATG 6 cut(s) 82, 337, 496, 514, 715, 884
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 47, 56
DpnII GATC 2 cut(s) 45, 54
EciI GGCGGA 4 cut(s) 91, 422, 465, 582
Eco130I CCWWGG 2 cut(s) 81, 336
Eco147I AGGCCT 1 cut(s) 721
Eco32I GATATC 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 369
EcoRII CCWGG 2 cut(s) 162, 721
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 2 cut(s) 81, 336
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 81, 336
FaeI CATG 6 cut(s) 85, 340, 499, 517, 718, 887
FaiI YATR 7 cut(s) 83, 317, 338, 497, 515, 716, 885
FaqI GGGAC 2 cut(s) 246, 725
FatI CATG 6 cut(s) 81, 336, 495, 513, 714, 883
FauI CCCGC 7 cut(s) 98, 179, 246, 606, 610, 658, 719
Fnu4HI GCNGC 6 cut(s) 78, 357, 360, 663, 812, 822
FokI GGATG 5 cut(s) 257, 401, 772, 782, 878
Fsp4HI GCNGC 6 cut(s) 78, 357, 360, 663, 812, 822
FspBI CTAG 1 cut(s) 420
GlaI GCGC 4 cut(s) 169, 363, 365, 367
GluI GCNGC 6 cut(s) 78, 357, 360, 663, 812, 822
GsaI CCCAGC 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 86, 288, 721
HapII CCGG 6 cut(s) 105, 242, 447, 459, 525, 815
HgaI GACGC 2 cut(s) 265, 487
HhaI GCGC 4 cut(s) 170, 364, 366, 368
Hin1I GRCGYC 3 cut(s) 168, 276, 479
Hin1II CATG 6 cut(s) 85, 340, 499, 517, 718, 887
Hin6I GCGC 4 cut(s) 168, 362, 364, 366
HinP1I GCGC 4 cut(s) 168, 362, 364, 366
HincII GTYRAC 2 cut(s) 477, 672
HindII GTYRAC 2 cut(s) 477, 672
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 1 cut(s) 182
HpaII CCGG 6 cut(s) 105, 242, 447, 459, 525, 815
HphI GGTGA 3 cut(s) 109, 214, 757
Hpy166II GTNNAC 2 cut(s) 477, 672
Hpy188I TCNGA 1 cut(s) 537
Hpy8I GTNNAC 2 cut(s) 477, 672
HpyAV CCTTC 2 cut(s) 587, 742
HpyCH4III ACNGT 2 cut(s) 302, 310
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 4 cut(s) 77, 200, 543, 734
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 365, 614, 854, 924
HpySE526I ACGT 1 cut(s) 411
Hsp92I GRCGYC 3 cut(s) 168, 276, 479
Hsp92II CATG 6 cut(s) 85, 340, 499, 517, 718, 887
HspAI GCGC 4 cut(s) 168, 362, 364, 366
KasI GGCGCC 1 cut(s) 167
KroI GCCGGC 2 cut(s) 104, 446
KroNI GCCGGC 2 cut(s) 106, 448
Kzo9I GATC 2 cut(s) 45, 54
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 3 cut(s) 89, 343, 798
LweI GCATC 2 cut(s) 379, 445
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 304, 505
MalI GATC 2 cut(s) 47, 56
MauBI CGCGCGCG 1 cut(s) 362
MbiI CCGCTC 1 cut(s) 613
MboI GATC 2 cut(s) 45, 54
Mly113I GGCGCC 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 603
MroNI GCCGGC 2 cut(s) 104, 446
MseI TTAA 2 cut(s) 68, 827
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 6 cut(s) 105, 242, 447, 459, 525, 815
MspR9I CCNGG 4 cut(s) 164, 526, 723, 816
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 2 cut(s) 164, 723
MvnI CGCG 3 cut(s) 362, 364, 366
MwoI GCNNNNNNNGC 5 cut(s) 83, 365, 614, 854, 924
NaeI GCCGGC 2 cut(s) 106, 448
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 2 cut(s) 526, 816
NcoI CCATGG 2 cut(s) 81, 336
NdeII GATC 2 cut(s) 45, 54
NgoMIV GCCGGC 2 cut(s) 104, 446
NlaIII CATG 6 cut(s) 85, 340, 499, 517, 718, 887
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 227
PasI CCCWGGG 1 cut(s) 163
PauI GCGCGC 2 cut(s) 362, 364
PceI AGGCCT 1 cut(s) 721
PctI GAATGC 1 cut(s) 879
PdiI GCCGGC 2 cut(s) 106, 448
PfeI GAWTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 6 cut(s) 79, 358, 361, 664, 813, 823
PluTI GGCGCC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 162, 721
PspFI CCCAGC 1 cut(s) 378
PspGI CCWGG 2 cut(s) 162, 721
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 401
PteI GCGCGC 2 cut(s) 362, 364
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 2 cut(s) 68, 827
SatI GCNGC 6 cut(s) 78, 357, 360, 663, 812, 822
Sau3AI GATC 2 cut(s) 45, 54
Sau96I GGNCC 1 cut(s) 286
ScrFI CCNGG 4 cut(s) 164, 526, 723, 816
SetI ASST 3 cut(s) 414, 512, 876
SfaNI GCATC 2 cut(s) 379, 445
SfoI GGCGCC 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 227
SseBI AGGCCT 1 cut(s) 721
SspDI GGCGCC 1 cut(s) 167
SspI AATATT 1 cut(s) 441
SspMI CTAG 1 cut(s) 420
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 4 cut(s) 162, 524, 721, 814
StyI CCWWGG 2 cut(s) 81, 336
TaaI ACNGT 2 cut(s) 302, 310
TaiI ACGT 1 cut(s) 414
TaqI TCGA 1 cut(s) 250
TauI GCSGC 3 cut(s) 362, 665, 824
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 2 cut(s) 68, 827
Tru9I TTAA 2 cut(s) 68, 827
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 77, 356, 811
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 872
TspGWI ACGGA 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 401
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.