MD15G1424900.v1.1

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004641
Physical Location & Seq
Forward (+)
52605001 .. 52605924
924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1424900.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGGAACTCCTTACCCAAACCCCATCTCTCCTACCCGAACCCGCGTGGGCTGGATTGGCATCGGCGTCATGGGCGCCGCCATGGCCACCCGTCTTCTCTCCACCGGCTACTCCGTCACCGTCTACGCTCGCACTCCTTCCAAAGCTCAACCACTACAGTCCCTCGGTGCCCAACTCGCCGATTCCCCTTTTCATCTTGCTCAGCTATGCGACGTCGTCTTCACCATGGTGGGACACCCACAAGATGTGCGATCAAATGTCCTCCACCCAGAAACCGGCATTCTATCCGGCATTAGTCCCAACTCCGTCACCGTCGACATGACTAGCAGTCATCCGGCCCTTGCGCGCGAGATATTCACTGCTGCGCGCGCCAAAAATTGTTGGTCCGTCGATGCGCCGGTGTCGGGTGGCGACATTGGCGCTAGAGAAGGCAAACTCGCCATTCTTGCGGGCGGTGATGCTTCCGTTGTCGAGTGGTTGGCCCCGTTGTTTGACATTTTGGGGAAGGCTACTTATGTGGGGCCTGCCGGTTCTGGCCAGAGTTGCAAGATTGCGAACCAGATTGTAGTGGGAGGGAATTTGCTAGGACTGAGTGAAGGGTTGGTGTTTGCGGAGCAGGCGGGGTTGGATGTGCGGCAGTTTATGGAGGCGGTGAGGGGCGGGGCCGCGGGGTCAATGGTGATGGAGTTGTTTGGGGAGAGGATGATTGGGAGGGACTTCAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGTGTGGATGTTGTGGAGGAGAGTGAGGATGAGAGAGTGGTGGTGTTACCTGGTGCTGCATTGTGCAAGCAGTTATATTCCGGCATGGTGGCGAATGGAGACGGGAAGCTTGGAACTCAAGGTCTTATCACTGCCATAGAGAGGCTCAATGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

308

Amino Acids

32.02

Weight (kDa)

5.45

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 1e-44 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 83 4.6e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 177 - 299 1.1e-26 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccB1I GGYRCC 2 cut(s) 74, 167
AccI GTMKAC 2 cut(s) 123, 315
AccII CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
AcoI YGGCCR 2 cut(s) 84, 535
AcsI RAATTY 1 cut(s) 577
AcuI CTGAAG 1 cut(s) 703
AcyI GRCGYC 3 cut(s) 66, 75, 213
AfiI CCNNNNNNNGG 3 cut(s) 275, 404, 906
AhdI GACNNNNNGTC 1 cut(s) 327
AjnI CCWGG 2 cut(s) 724, 814
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 3 cut(s) 146, 205, 874
AluI AGCT 3 cut(s) 146, 205, 874
Alw26I GTCTC 1 cut(s) 858
AoxI GGCC 7 cut(s) 84, 336, 480, 521, 535, 663, 722
ApeKI GCWGC 2 cut(s) 362, 821
ApoI RAATTY 1 cut(s) 577
AspLEI GCGC 8 cut(s) 77, 346, 348, 367, 369, 371, 397, 422
AspS9I GGNCC 5 cut(s) 337, 384, 481, 521, 663
AsuHPI GGTGA 7 cut(s) 109, 214, 301, 467, 664, 691, 760
AvaII GGWCC 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 2 cut(s) 86, 537
BanI GGYRCC 2 cut(s) 74, 167
BbsI GAAGAC 2 cut(s) 86, 211
BbvI GCAGC 2 cut(s) 349, 808
BccI CCATC 2 cut(s) 32, 676
BciT130I CCWGG 2 cut(s) 726, 816
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 3 cut(s) 324, 423, 584
BfmI CTRYAG 1 cut(s) 155
BfoI RGCGCY 2 cut(s) 78, 423
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 5 cut(s) 78, 363, 635, 666, 822
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 5 cut(s) 79, 364, 636, 667, 823
Bme1390I CCNGG 2 cut(s) 726, 816
Bme18I GGWCC 1 cut(s) 384
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgT120I GGNCC 5 cut(s) 337, 384, 481, 521, 663
BmiI GGNNCC 5 cut(s) 76, 169, 483, 522, 664
BmrFI CCNGG 2 cut(s) 726, 816
BmsI GCATC 3 cut(s) 69, 382, 448
BpiI GAAGAC 2 cut(s) 86, 211
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 867
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 3 cut(s) 66, 75, 213
BsaJI CCNNGG 4 cut(s) 81, 163, 225, 666
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 404, 906
Bse118I RCCGGY 4 cut(s) 104, 275, 397, 527
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 2 cut(s) 726, 816
BseDI CCNNGG 4 cut(s) 81, 163, 225, 666
BseGI GGATG 5 cut(s) 331, 634, 708, 778, 799
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 3 cut(s) 275, 404, 906
BseMII CTCAG 3 cut(s) 215, 581, 729
BsePI GCGCGC 3 cut(s) 344, 365, 367
BseRI GAGGAG 1 cut(s) 797
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 2 cut(s) 349, 808
Bsh1236I CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
BshFI GGCC 7 cut(s) 86, 338, 482, 523, 537, 665, 724
BshNI GGYRCC 2 cut(s) 74, 167
BsiSI CCGG 7 cut(s) 105, 276, 288, 335, 398, 528, 846
BslFI GGGAC 4 cut(s) 145, 246, 282, 728
BslI CCNNNNNNNGG 3 cut(s) 275, 404, 906
BsmAI GTCTC 1 cut(s) 858
BsmBI CGTCTC 1 cut(s) 858
BsmFI GGGAC 4 cut(s) 145, 246, 282, 728
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 7 cut(s) 86, 338, 482, 523, 537, 665, 724
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 1 cut(s) 251
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 2 cut(s) 81, 225
BspANI GGCC 7 cut(s) 86, 338, 482, 523, 537, 665, 724
BspCNI CTCAG 3 cut(s) 214, 582, 730
BspFNI CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
BspLI GGNNCC 5 cut(s) 76, 169, 483, 522, 664
BspT107I GGYRCC 2 cut(s) 74, 167
BsrFI RCCGGY 4 cut(s) 104, 275, 397, 527
BssAI RCCGGY 4 cut(s) 104, 275, 397, 527
BssECI CCNNGG 4 cut(s) 81, 163, 225, 666
BssHII GCGCGC 3 cut(s) 344, 365, 367
BssMI GATC 1 cut(s) 251
BssNI GRCGYC 3 cut(s) 66, 75, 213
BssT1I CCWWGG 2 cut(s) 81, 225
Bst2UI CCWGG 2 cut(s) 726, 816
Bst4CI ACNGT 3 cut(s) 121, 159, 313
BstACI GRCGYC 3 cut(s) 66, 75, 213
BstC8I GCNNGC 8 cut(s) 130, 346, 367, 369, 451, 525, 618, 833
BstDEI CTNAG 3 cut(s) 201, 590, 738
BstDSI CCRYGG 3 cut(s) 81, 225, 666
BstF5I GGATG 5 cut(s) 331, 634, 708, 778, 799
BstFNI CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
BstH2I RGCGCY 2 cut(s) 78, 423
BstHHI GCGC 8 cut(s) 77, 346, 348, 367, 369, 371, 397, 422
BstKTI GATC 1 cut(s) 254
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 1 cut(s) 251
BstMWI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 368, 417, 446, 543, 617
BstNI CCWGG 2 cut(s) 726, 816
BstSCI CCNGG 2 cut(s) 724, 814
BstSFI CTRYAG 1 cut(s) 155
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
BstV1I GCAGC 2 cut(s) 349, 808
BstV2I GAAGAC 2 cut(s) 86, 211
BsuRI GGCC 7 cut(s) 86, 338, 482, 523, 537, 665, 724
BtgI CCRYGG 3 cut(s) 81, 225, 666
BtsCI GGATG 5 cut(s) 331, 634, 708, 778, 799
BtsI GCAGTG 2 cut(s) 357, 894
BtsIMutI CAGTG 2 cut(s) 357, 894
Cac8I GCNNGC 8 cut(s) 130, 346, 367, 369, 451, 525, 618, 833
CfoI GCGC 8 cut(s) 77, 346, 348, 367, 369, 371, 397, 422
Cfr10I RCCGGY 4 cut(s) 104, 275, 397, 527
Cfr13I GGNCC 5 cut(s) 337, 384, 481, 521, 663
Cfr42I CCGCGG 1 cut(s) 669
CseI GACGC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 814
CviAII CATG 5 cut(s) 70, 82, 226, 319, 850
DdeI CTNAG 3 cut(s) 201, 590, 738
DinI GGCGCC 1 cut(s) 76
DpnI GATC 1 cut(s) 253
DpnII GATC 1 cut(s) 251
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 2 cut(s) 84, 535
Eam1105I GACNNNNNGTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 81, 225
Eco147I AGGCCT 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 724, 814
EcoT14I CCWWGG 2 cut(s) 81, 225
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 81, 225
Esp3I CGTCTC 1 cut(s) 858
FaeI CATG 5 cut(s) 73, 85, 229, 322, 853
FaqI GGGAC 4 cut(s) 145, 246, 282, 728
FatI CATG 5 cut(s) 69, 81, 225, 318, 849
FauI CCCGC 5 cut(s) 50, 442, 613, 653, 661
FblI GTMKAC 2 cut(s) 123, 315
Fnu4HI GCNGC 5 cut(s) 78, 363, 635, 666, 822
FokI GGATG 5 cut(s) 318, 641, 715, 785, 806
Fsp4HI GCNGC 5 cut(s) 78, 363, 635, 666, 822
FspBI CTAG 3 cut(s) 324, 423, 584
GlaI GCGC 8 cut(s) 76, 345, 347, 366, 368, 370, 396, 421
GluI GCNGC 5 cut(s) 78, 363, 635, 666, 822
HaeII RGCGCY 2 cut(s) 78, 423
HaeIII GGCC 7 cut(s) 86, 338, 482, 523, 537, 665, 724
HapII CCGG 7 cut(s) 105, 276, 288, 335, 398, 528, 846
HgaI GACGC 1 cut(s) 55
HhaI GCGC 8 cut(s) 77, 346, 348, 367, 369, 371, 397, 422
Hin1I GRCGYC 3 cut(s) 66, 75, 213
Hin1II CATG 5 cut(s) 73, 85, 229, 322, 853
Hin6I GCGC 8 cut(s) 75, 344, 346, 365, 367, 369, 395, 420
HinP1I GCGC 8 cut(s) 75, 344, 346, 365, 367, 369, 395, 420
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 1 cut(s) 182
HpaII CCGG 7 cut(s) 105, 276, 288, 335, 398, 528, 846
HphI GGTGA 7 cut(s) 109, 214, 301, 467, 664, 691, 760
Hpy166II GTNNAC 2 cut(s) 124, 316
Hpy8I GTNNAC 2 cut(s) 124, 316
Hpy99I CGWCG 4 cut(s) 215, 218, 317, 392
HpyAV CCTTC 5 cut(s) 147, 422, 499, 590, 745
HpyCH4III ACNGT 3 cut(s) 121, 159, 313
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 546, 824, 831
HpyF10VI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 368, 417, 446, 543, 617
HpyF3I CTNAG 3 cut(s) 201, 590, 738
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 3 cut(s) 66, 75, 213
Hsp92II CATG 5 cut(s) 73, 85, 229, 322, 853
HspAI GCGC 8 cut(s) 75, 344, 346, 365, 367, 369, 395, 420
KasI GGCGCC 1 cut(s) 74
KspI CCGCGG 1 cut(s) 669
Kzo9I GATC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 2 cut(s) 349, 808
LweI GCATC 3 cut(s) 69, 382, 448
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 3 cut(s) 324, 423, 584
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 115, 307, 810
MalI GATC 1 cut(s) 253
MboI GATC 1 cut(s) 251
MboII GAAGA 2 cut(s) 86, 211
MhlI GDGCHC 1 cut(s) 172
MlsI TGGCCA 2 cut(s) 86, 537
MluCI AATT 2 cut(s) 376, 577
MluNI TGGCCA 2 cut(s) 86, 537
Mly113I GGCGCC 1 cut(s) 75
MmeI TCCRAC 1 cut(s) 606
Mox20I TGGCCA 2 cut(s) 86, 537
MscI TGGCCA 2 cut(s) 86, 537
MslI CAYNNNNRTG 1 cut(s) 227
Msp20I TGGCCA 2 cut(s) 86, 537
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 7 cut(s) 105, 276, 288, 335, 398, 528, 846
MspR9I CCNGG 2 cut(s) 726, 816
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 2 cut(s) 726, 816
MvnI CGCG 6 cut(s) 45, 346, 348, 367, 369, 668
MwoI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 368, 417, 446, 543, 617
NarI GGCGCC 1 cut(s) 75
NcoI CCATGG 2 cut(s) 81, 225
NdeII GATC 1 cut(s) 251
NlaIII CATG 5 cut(s) 73, 85, 229, 322, 853
NlaIV GGNNCC 5 cut(s) 76, 169, 483, 522, 664
NmuCI GTSAC 2 cut(s) 115, 307
OliI CACNNNNGTG 1 cut(s) 227
PauI GCGCGC 3 cut(s) 344, 365, 367
PceI AGGCCT 1 cut(s) 724
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 182
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 5 cut(s) 79, 364, 636, 667, 823
PluTI GGCGCC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 724, 814
PspGI CCWGG 2 cut(s) 724, 814
PspN4I GGNNCC 5 cut(s) 76, 169, 483, 522, 664
PspPI GGNCC 5 cut(s) 337, 384, 481, 521, 663
PsyI GACNNNGTC 1 cut(s) 215
PteI GCGCGC 3 cut(s) 344, 365, 367
RseI CAYNNNNRTG 1 cut(s) 227
SacII CCGCGG 1 cut(s) 669
SalI GTCGAC 1 cut(s) 314
SatI GCNGC 5 cut(s) 78, 363, 635, 666, 822
Sau3AI GATC 1 cut(s) 251
Sau96I GGNCC 5 cut(s) 337, 384, 481, 521, 663
ScrFI CCNGG 2 cut(s) 726, 816
SduI GDGCHC 1 cut(s) 172
SetI ASST 6 cut(s) 148, 207, 216, 817, 876, 889
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 3 cut(s) 69, 382, 448
SfcI CTRYAG 1 cut(s) 155
SfoI GGCGCC 1 cut(s) 76
Sfr303I CCGCGG 1 cut(s) 669
SgrAI CRCCGGYG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 669
SinI GGWCC 1 cut(s) 384
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 882
SmoI CTYRAG 1 cut(s) 882
Sse9I AATT 2 cut(s) 376, 577
SseBI AGGCCT 1 cut(s) 724
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 3 cut(s) 324, 423, 584
StuI AGGCCT 1 cut(s) 724
StyD4I CCNGG 2 cut(s) 724, 814
StyI CCWWGG 2 cut(s) 81, 225
TaaI ACNGT 3 cut(s) 121, 159, 313
TaiI ACGT 1 cut(s) 216
TaqI TCGA 3 cut(s) 315, 390, 471
TasI AATT 2 cut(s) 376, 577
TauI GCSGC 3 cut(s) 80, 637, 668
TfiI GAWTC 1 cut(s) 182
TscAI CASTG 2 cut(s) 364, 901
TseFI GTSAC 2 cut(s) 115, 307
TseI GCWGC 2 cut(s) 362, 821
Tsp45I GTSAC 2 cut(s) 115, 307
TspDTI ATGAA 1 cut(s) 182
TspGWI ACGGA 4 cut(s) 103, 295, 376, 454
TspRI CASTG 2 cut(s) 364, 901
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 384
XapI RAATTY 1 cut(s) 577
XmiI GTMKAC 2 cut(s) 123, 315
XspI CTAG 3 cut(s) 324, 423, 584
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.