Rroxscaffold_3G00237440

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004641
Physical Location & Seq
Forward (+)
25028036 .. 25028956
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237440.1

Sequence Viewer

Length: 921 bp
ATGGGGACTCCTTACCCAAACCCCATCTCTCCTGCCCACACCCGCATAGGCTGGATTGGCATCGGCGTAATGGGTGCCGCCATGGCCTGCCGCCTTATCTCAGCCGGCTACTCCCTCACCGTCTACGCTCGCACTCCTTCCAAAGCCGCTCCACTACAATCCCAGGGGGCCCACCTCGCCGACTCCCCATTTCAACTCGCCCAACGCGCCGACGTCGTCTTCACCATGGTGGGCCACCCGTCCGATGTCCGATCAAATGTCCTCGGCCCAAACGCCCTTCTTTCCGGCCTCAATCCCAACTGCGTCACCGTCGACATGACCAGCAGCCATCCCGCCCTCGCGCGTGAGATTTTCGCCGCCGCACGCGCCAAGGACTGCTGGTCTGTCGACGCGCCGGTGTCCGGCGGTGACATTGGCGCCAGGGAAGGCAAGCTTGCTATACTCGCCGGTGGGGATGCCTCGGTGGTGAAGTGGTTGGCGCCATTGTTTGAAATTATGGGGAAGGTTACTTATATGGGTCCGGCAGGGTGCGGTCAGAGTTGTAAAATTGGTAACCAGATCATTGTGGGGGCGAATTTGCTTGGATTGAGTGAAGGGTTGGTGTTTGCGGAGCGGGCCGGGTTGGATGTGAGGCGGTTTATGGAGGCGGTGAGGGGCGGGGCGGCGGGGTCCAAAGTAATGGAGTTGTTTGGGGAGAGGATGATTGAGAGGGACTTCAGGCCTGGTGGTTTTGCTGAGTACATGGTGAAGGATATGGGGATGGGAGTGGATGTTGTGGAGGAAGGTGAGGATGAGAAAGTAGTAGTGTTGCCGGGTGCTGCATTGTGCAAGCAGCTGTTTTCGGGTATGGTGGCTAATGGGGACGGGAAGCTTGGTACGCAAGGTCTTATCACTGCTATAGAGAGGCTCAATGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

306

Amino Acids

31.92

Weight (kDa)

6.44

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 1.6e-46 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 77 2.3e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 298 9.2e-26 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccB1I GGYRCC 3 cut(s) 74, 416, 478
AccBSI CCGCTC 2 cut(s) 149, 613
AccI GTMKAC 3 cut(s) 123, 312, 387
AccII CGCG 5 cut(s) 207, 341, 343, 366, 392
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 700
AcyI GRCGYC 3 cut(s) 213, 417, 479
AfaI GTAC 2 cut(s) 740, 877
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 2 cut(s) 194, 491
AhdI GACNNNNNGTC 1 cut(s) 379
AjnI CCWGG 3 cut(s) 162, 419, 721
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 3 cut(s) 433, 835, 871
AluI AGCT 3 cut(s) 433, 835, 871
AoxI GGCC 7 cut(s) 84, 168, 232, 265, 286, 615, 719
ApaI GGGCCC 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 324, 818, 832
ApoI RAATTY 1 cut(s) 574
AspLEI GCGC 6 cut(s) 209, 343, 368, 394, 419, 481
AspS9I GGNCC 7 cut(s) 168, 169, 232, 266, 518, 615, 669
AsuC2I CCSGG 2 cut(s) 619, 813
AsuHPI GGTGA 8 cut(s) 109, 214, 298, 419, 478, 661, 757, 797
AvaII GGWCC 2 cut(s) 518, 669
BaeGI GKGCMC 1 cut(s) 172
BanI GGYRCC 3 cut(s) 74, 416, 478
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 3 cut(s) 336, 805, 844
BccI CCATC 3 cut(s) 32, 336, 754
BciT130I CCWGG 3 cut(s) 164, 421, 723
BcnI CCSGG 2 cut(s) 619, 813
BfmI CTRYAG 1 cut(s) 897
BfoI RGCGCY 2 cut(s) 420, 482
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 9 cut(s) 78, 91, 147, 325, 357, 360, 663, 819, 833
BlsI GCNGC 9 cut(s) 79, 92, 148, 326, 358, 361, 664, 820, 834
Bme1390I CCNGG 5 cut(s) 164, 421, 619, 723, 813
Bme18I GGWCC 2 cut(s) 518, 669
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgT120I GGNCC 7 cut(s) 168, 169, 232, 266, 518, 615, 669
BmiI GGNNCC 7 cut(s) 76, 169, 170, 418, 480, 519, 670
BmrFI CCNGG 5 cut(s) 164, 421, 619, 723, 813
BmsI GCATC 2 cut(s) 69, 445
BpiI GAAGAC 1 cut(s) 211
BpuMI CCSGG 2 cut(s) 619, 813
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 3 cut(s) 213, 417, 479
BsaJI CCNNGG 8 cut(s) 81, 162, 163, 225, 262, 369, 420, 459
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 3 cut(s) 104, 394, 446
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 3 cut(s) 164, 421, 723
BseDI CCNNGG 8 cut(s) 81, 162, 163, 225, 262, 369, 420, 459
BseGI GGATG 7 cut(s) 328, 460, 631, 705, 765, 775, 796
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 114, 726
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 3 cut(s) 336, 805, 844
Bsh1236I CGCG 5 cut(s) 207, 341, 343, 366, 392
BshFI GGCC 7 cut(s) 86, 170, 234, 267, 288, 617, 721
BshNI GGYRCC 3 cut(s) 74, 416, 478
BsiSI CCGG 8 cut(s) 105, 285, 395, 402, 447, 521, 618, 812
BslFI GGGAC 3 cut(s) 19, 725, 875
BslI CCNNNNNNNGG 1 cut(s) 401
BsmFI GGGAC 3 cut(s) 19, 725, 875
BsnI GGCC 7 cut(s) 86, 170, 234, 267, 288, 617, 721
Bsp120I GGGCCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 2 cut(s) 251, 558
Bsp19I CCATGG 2 cut(s) 81, 225
BspANI GGCC 7 cut(s) 86, 170, 234, 267, 288, 617, 721
BspCNI CTCAG 2 cut(s) 113, 727
BspFNI CGCG 5 cut(s) 207, 341, 343, 366, 392
BspLI GGNNCC 7 cut(s) 76, 169, 170, 418, 480, 519, 670
BspT107I GGYRCC 3 cut(s) 74, 416, 478
BsrBI CCGCTC 2 cut(s) 149, 613
BsrFI RCCGGY 3 cut(s) 104, 394, 446
BssAI RCCGGY 3 cut(s) 104, 394, 446
BssECI CCNNGG 8 cut(s) 81, 162, 163, 225, 262, 369, 420, 459
BssMI GATC 2 cut(s) 251, 558
BssNI GRCGYC 3 cut(s) 213, 417, 479
BssT1I CCWWGG 3 cut(s) 81, 225, 369
Bst2UI CCWGG 3 cut(s) 164, 421, 723
Bst4CI ACNGT 2 cut(s) 121, 310
BstACI GRCGYC 3 cut(s) 213, 417, 479
BstC8I GCNNGC 8 cut(s) 88, 106, 130, 364, 431, 435, 615, 830
BstDEI CTNAG 2 cut(s) 100, 735
BstDSI CCRYGG 2 cut(s) 81, 225
BstEII GGTNACC 1 cut(s) 551
BstF5I GGATG 7 cut(s) 328, 460, 631, 705, 765, 775, 796
BstFNI CGCG 5 cut(s) 207, 341, 343, 366, 392
BstH2I RGCGCY 2 cut(s) 420, 482
BstHHI GCGC 6 cut(s) 209, 343, 368, 394, 419, 481
BstKTI GATC 2 cut(s) 254, 561
BstMBI GATC 2 cut(s) 251, 558
BstMWI GCNNNNNNNGC 8 cut(s) 57, 83, 176, 206, 365, 443, 614, 877
BstNI CCWGG 3 cut(s) 164, 421, 723
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 5 cut(s) 162, 419, 617, 721, 811
BstSFI CTRYAG 1 cut(s) 897
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 5 cut(s) 207, 341, 343, 366, 392
BstV1I GCAGC 3 cut(s) 336, 805, 844
BstV2I GAAGAC 1 cut(s) 211
BstXI CCANNNNNNTGG 1 cut(s) 679
BsuRI GGCC 7 cut(s) 86, 170, 234, 267, 288, 617, 721
BtgI CCRYGG 2 cut(s) 81, 225
BtsCI GGATG 7 cut(s) 328, 460, 631, 705, 765, 775, 796
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 1 cut(s) 891
Cac8I GCNNGC 8 cut(s) 88, 106, 130, 364, 431, 435, 615, 830
CfoI GCGC 6 cut(s) 209, 343, 368, 394, 419, 481
Cfr10I RCCGGY 3 cut(s) 104, 394, 446
Cfr13I GGNCC 7 cut(s) 168, 169, 232, 266, 518, 615, 669
CseI GACGC 2 cut(s) 292, 398
Csp6I GTAC 2 cut(s) 739, 876
CviAII CATG 4 cut(s) 82, 226, 316, 742
CviQI GTAC 2 cut(s) 739, 876
DdeI CTNAG 2 cut(s) 100, 735
DinI GGCGCC 2 cut(s) 418, 480
DpnI GATC 2 cut(s) 253, 560
DpnII GATC 2 cut(s) 251, 558
DriI GACNNNNNGTC 1 cut(s) 379
Eam1105I GACNNNNNGTC 1 cut(s) 379
Eco130I CCWWGG 3 cut(s) 81, 225, 369
Eco147I AGGCCT 1 cut(s) 721
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 518, 669
Eco57I CTGAAG 1 cut(s) 700
Eco91I GGTNACC 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 168
EcoO65I GGTNACC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 162, 419, 721
EcoT14I CCWWGG 3 cut(s) 81, 225, 369
EcoT38I GRGCYC 1 cut(s) 172
EgeI GGCGCC 2 cut(s) 418, 480
EheI GGCGCC 2 cut(s) 418, 480
ErhI CCWWGG 3 cut(s) 81, 225, 369
FaeI CATG 4 cut(s) 85, 229, 319, 745
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 3 cut(s) 19, 725, 875
FatI CATG 4 cut(s) 81, 225, 315, 741
FauI CCCGC 5 cut(s) 50, 340, 606, 650, 658
FblI GTMKAC 3 cut(s) 123, 312, 387
Fnu4HI GCNGC 9 cut(s) 78, 91, 147, 325, 357, 360, 663, 819, 833
FokI GGATG 7 cut(s) 315, 467, 638, 712, 772, 782, 803
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 9 cut(s) 78, 91, 147, 325, 357, 360, 663, 819, 833
GlaI GCGC 6 cut(s) 208, 342, 367, 393, 418, 480
GluI GCNGC 9 cut(s) 78, 91, 147, 325, 357, 360, 663, 819, 833
HaeII RGCGCY 2 cut(s) 420, 482
HaeIII GGCC 7 cut(s) 86, 170, 234, 267, 288, 617, 721
HapII CCGG 8 cut(s) 105, 285, 395, 402, 447, 521, 618, 812
HgaI GACGC 2 cut(s) 292, 398
HhaI GCGC 6 cut(s) 209, 343, 368, 394, 419, 481
Hin1I GRCGYC 3 cut(s) 213, 417, 479
Hin1II CATG 4 cut(s) 85, 229, 319, 745
Hin6I GCGC 6 cut(s) 207, 341, 366, 392, 417, 479
HinP1I GCGC 6 cut(s) 207, 341, 366, 392, 417, 479
HincII GTYRAC 2 cut(s) 313, 388
HindII GTYRAC 2 cut(s) 313, 388
HindIII AAGCTT 2 cut(s) 431, 869
HinfI GANTC 2 cut(s) 7, 182
HpaII CCGG 8 cut(s) 105, 285, 395, 402, 447, 521, 618, 812
HphI GGTGA 8 cut(s) 109, 214, 298, 419, 478, 661, 757, 797
Hpy166II GTNNAC 3 cut(s) 124, 313, 388
Hpy188I TCNGA 3 cut(s) 244, 251, 537
Hpy8I GTNNAC 3 cut(s) 124, 313, 388
Hpy99I CGWCG 4 cut(s) 215, 218, 314, 392
HpyAV CCTTC 7 cut(s) 147, 287, 419, 496, 587, 742, 776
HpyCH4III ACNGT 2 cut(s) 121, 310
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 2 cut(s) 821, 828
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 83, 176, 206, 365, 443, 614, 877
HpyF3I CTNAG 2 cut(s) 100, 735
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 3 cut(s) 213, 417, 479
Hsp92II CATG 4 cut(s) 85, 229, 319, 745
HspAI GCGC 6 cut(s) 207, 341, 366, 392, 417, 479
KasI GGCGCC 2 cut(s) 416, 478
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 251, 558
LmnI GCTCC 2 cut(s) 154, 610
Lsp1109I GCAGC 3 cut(s) 336, 805, 844
LweI GCATC 2 cut(s) 69, 445
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 304, 407, 505, 551
MalI GATC 2 cut(s) 253, 560
MbiI CCGCTC 2 cut(s) 149, 613
MboI GATC 2 cut(s) 251, 558
MboII GAAGA 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 3 cut(s) 492, 546, 574
Mly113I GGCGCC 2 cut(s) 417, 479
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 8 cut(s) 105, 285, 395, 402, 447, 521, 618, 812
MspR9I CCNGG 5 cut(s) 164, 421, 619, 723, 813
MvaI CCWGG 3 cut(s) 164, 421, 723
MvnI CGCG 5 cut(s) 207, 341, 343, 366, 392
MwoI GCNNNNNNNGC 8 cut(s) 57, 83, 176, 206, 365, 443, 614, 877
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 2 cut(s) 417, 479
NciI CCSGG 2 cut(s) 619, 813
NcoI CCATGG 2 cut(s) 81, 225
NdeII GATC 2 cut(s) 251, 558
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 4 cut(s) 85, 229, 319, 745
NlaIV GGNNCC 7 cut(s) 76, 169, 170, 418, 480, 519, 670
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 304, 407
OliI CACNNNNGTG 1 cut(s) 227
PasI CCCWGGG 1 cut(s) 163
PceI AGGCCT 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 9 cut(s) 79, 92, 148, 326, 358, 361, 664, 820, 834
PleI GAGTC 1 cut(s) 176
PluTI GGCGCC 2 cut(s) 420, 482
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 3 cut(s) 162, 419, 721
PspEI GGTNACC 1 cut(s) 551
PspGI CCWGG 3 cut(s) 162, 419, 721
PspN4I GGNNCC 7 cut(s) 76, 169, 170, 418, 480, 519, 670
PspOMI GGGCCC 1 cut(s) 168
PspPI GGNCC 7 cut(s) 168, 169, 232, 266, 518, 615, 669
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 2 cut(s) 740, 877
RsaNI GTAC 2 cut(s) 739, 876
RseI CAYNNNNRTG 1 cut(s) 227
SalI GTCGAC 2 cut(s) 311, 386
SatI GCNGC 9 cut(s) 78, 91, 147, 325, 357, 360, 663, 819, 833
Sau3AI GATC 2 cut(s) 251, 558
Sau96I GGNCC 7 cut(s) 168, 169, 232, 266, 518, 615, 669
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 5 cut(s) 164, 421, 619, 723, 813
SduI GDGCHC 1 cut(s) 172
SetI ASST 8 cut(s) 177, 216, 435, 507, 787, 837, 873, 886
SfaNI GCATC 2 cut(s) 69, 445
SfcI CTRYAG 1 cut(s) 897
SfoI GGCGCC 2 cut(s) 418, 480
SgrAI CRCCGGYG 2 cut(s) 394, 446
SinI GGWCC 2 cut(s) 518, 669
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 3 cut(s) 492, 546, 574
SseBI AGGCCT 1 cut(s) 721
SspDI GGCGCC 2 cut(s) 416, 478
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 5 cut(s) 162, 419, 617, 721, 811
StyI CCWWGG 3 cut(s) 81, 225, 369
TaaI ACNGT 2 cut(s) 121, 310
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 312, 387
TasI AATT 3 cut(s) 492, 546, 574
TatI WGTACW 1 cut(s) 738
TauI GCSGC 6 cut(s) 80, 93, 149, 359, 362, 665
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 2 cut(s) 304, 407
TseI GCWGC 3 cut(s) 324, 818, 832
Tsp45I GTSAC 2 cut(s) 304, 407
TspRI CASTG 1 cut(s) 898
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 2 cut(s) 518, 669
XapI RAATTY 1 cut(s) 574
XmiI GTMKAC 3 cut(s) 123, 312, 387
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.