RLG00000002096

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004641
Physical Location & Seq
Reverse (-)
25325982 .. 25326926
945 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002096

Sequence Viewer

Length: 945 bp
ATGGGAACTCCTTACCCAAACCCCATAACTCCTGCTCAGACCCGCATTGGCTGGATCGGCATTGGCCTCATGGGCGCTCCTATGGCTTCTCGCCTCATCTCAGCTGGTCACTCCCTCACCATCTATGCTCGCACTCCTTCCAAGGCCCTTTCACTACAATCCCAAGGTGCCCGCCTAGCCCAATCCCCTTCTGAAGTTGCTCAATGTAGTGATGTTGTCTTCACCATAGTGGGACACCCACCAGATGTACGAGAAGTTATCTTGGAGGCAAACGGCATCCTTTCAGGCCTAAACCCGAACGGCGTCACGGTGGACATGACCACCAATCTACCAGCCTTGGCACGTGAGATATCATCAGCTGCGCGTGCCAAAGATTGTTGGGCCGTTGACGCCCCAGTCTCTGGGGCAGATGTTGGCGCCAGGGAAGGGAAACTTGGAATATTTGCAGGCGGTGATGCTGGAGTCGTCAAGTGGTTAACACCATTGTTTGATATCATGGGAAAGGTTACGTATATGGGTGAAGCAGGGTGCGGCCAGAGCTGCAAGATAGCGAATAACATTGTAGGAGGGGTGAGTTTTGTCGGGTTGAGTGAAGGTCTGGTGTTTGCAGAGAGAGCTGGCTTGGATTTGAAGCAGTTTTTGGAAGCAGTGAGAGGAGGGGCGGCAGGATCGACGTTGATGGAGTTATTTGGGAAGAAAATGATCGAGAGGGACTTCAGGCCTGGTGGGTTTGTTGAGTATATAGTGAAGGACTTAGGGATGGGTTTAAATATTGTGGAGGAAGCTGATGATGAAAGGGTGGTGGTGTTGCCTGGTGCTGCATTGTGCAAGCAACTGTTTACTGGGATGGTTGCTAATGGGGATGCAAAGCTCGGCCTTCAGGGCCTTATCACTGTTATACAAAGGCTCAATGGCATATCTGAAATCGACAATTCTACTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

315

Amino Acids

32.77

Weight (kDa)

5.37

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 7.6e-45 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 90 3.6e-08 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 299 1.2e-24 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB1I GGYRCC 2 cut(s) 167, 416
AccB7I CCANNNNNTGG 1 cut(s) 401
AccII CGCG 1 cut(s) 364
AciI CCGC 5 cut(s) 43, 172, 450, 531, 662
AclWI GGATC 2 cut(s) 62, 676
AcoI YGGCCR 1 cut(s) 532
AcuI CTGAAG 3 cut(s) 213, 700, 863
AcvI CACGTG 1 cut(s) 344
AcyI GRCGYC 3 cut(s) 303, 390, 417
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 2 cut(s) 401, 426
AgsI TTSAA 1 cut(s) 631
AjnI CCWGG 3 cut(s) 419, 721, 811
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 623, 655
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 6 cut(s) 104, 359, 540, 617, 785, 871
AluI AGCT 6 cut(s) 104, 359, 540, 617, 785, 871
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 2 cut(s) 62, 676
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 8 cut(s) 64, 144, 286, 381, 532, 719, 874, 883
ApeKI GCWGC 3 cut(s) 359, 540, 818
AspLEI GCGC 3 cut(s) 77, 364, 419
AspS9I GGNCC 3 cut(s) 145, 381, 883
AsuHPI GGTGA 5 cut(s) 109, 214, 464, 530, 583
BaeGI GKGCMC 1 cut(s) 172
BanI GGYRCC 2 cut(s) 167, 416
BbrPI CACGTG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 3 cut(s) 346, 527, 805
BccI CCATC 4 cut(s) 128, 673, 754, 841
BceAI ACGGC 3 cut(s) 289, 316, 368
BciT130I CCWGG 3 cut(s) 421, 723, 813
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 176
BfoI RGCGCY 2 cut(s) 78, 420
BglI GCCNNNNNGGC 2 cut(s) 72, 882
BisI GCNGC 5 cut(s) 360, 532, 541, 663, 819
BlsI GCNGC 5 cut(s) 361, 533, 542, 664, 820
Bme1390I CCNGG 3 cut(s) 421, 723, 813
BmgT120I GGNCC 3 cut(s) 145, 381, 883
BmiI GGNNCC 2 cut(s) 169, 418
BmrFI CCNGG 3 cut(s) 421, 723, 813
BmrI ACTGGG 2 cut(s) 389, 852
BmsI GCATC 3 cut(s) 285, 445, 853
BmuI ACTGGG 2 cut(s) 389, 852
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 480
BsaAI YACGTR 2 cut(s) 344, 510
BsaHI GRCGYC 3 cut(s) 303, 390, 417
BsaJI CCNNGG 4 cut(s) 141, 163, 336, 420
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 426
Bse1I ACTGG 2 cut(s) 395, 847
BseBI CCWGG 3 cut(s) 421, 723, 813
BseDI CCNNGG 4 cut(s) 141, 163, 336, 420
BseGI GGATG 4 cut(s) 276, 765, 852, 868
BseLI CCNNNNNNNGG 2 cut(s) 401, 426
BseMII CTCAG 2 cut(s) 50, 114
BseNI ACTGG 2 cut(s) 395, 847
BseRI GAGGAG 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 3 cut(s) 346, 527, 805
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 8 cut(s) 66, 146, 288, 383, 534, 721, 876, 885
BshNI GGYRCC 2 cut(s) 167, 416
BslFI GGGAC 2 cut(s) 246, 725
BslI CCNNNNNNNGG 2 cut(s) 401, 426
BsmAI GTCTC 1 cut(s) 403
BsmFI GGGAC 2 cut(s) 246, 725
BsnI GGCC 8 cut(s) 66, 146, 288, 383, 534, 721, 876, 885
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 54, 668, 702
BspACI CCGC 5 cut(s) 43, 172, 450, 531, 662
BspANI GGCC 8 cut(s) 66, 146, 288, 383, 534, 721, 876, 885
BspCNI CTCAG 2 cut(s) 49, 113
BspFNI CGCG 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 169, 418
BspPI GGATC 2 cut(s) 62, 676
BspT107I GGYRCC 2 cut(s) 167, 416
BsrI ACTGG 2 cut(s) 395, 847
BssECI CCNNGG 4 cut(s) 141, 163, 336, 420
BssMI GATC 3 cut(s) 54, 668, 702
BssNI GRCGYC 3 cut(s) 303, 390, 417
BssT1I CCWWGG 3 cut(s) 141, 163, 336
Bst2UI CCWGG 3 cut(s) 421, 723, 813
Bst4CI ACNGT 3 cut(s) 310, 837, 895
BstACI GRCGYC 3 cut(s) 303, 390, 417
BstBAI YACGTR 2 cut(s) 344, 510
BstC8I GCNNGC 6 cut(s) 130, 172, 366, 448, 619, 830
BstDEI CTNAG 3 cut(s) 36, 100, 754
BstF5I GGATG 4 cut(s) 276, 765, 852, 868
BstFNI CGCG 1 cut(s) 364
BstH2I RGCGCY 2 cut(s) 78, 420
BstHHI GCGC 3 cut(s) 77, 364, 419
BstKTI GATC 3 cut(s) 57, 671, 705
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 54, 668, 702
BstNI CCWGG 3 cut(s) 421, 723, 813
BstSCI CCNGG 3 cut(s) 419, 721, 811
BstSLI GKGCMC 1 cut(s) 172
BstSNI TACGTA 1 cut(s) 510
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 3 cut(s) 346, 527, 805
BstV2I GAAGAC 1 cut(s) 211
BsuRI GGCC 8 cut(s) 66, 146, 288, 383, 534, 721, 876, 885
BtsCI GGATG 4 cut(s) 276, 765, 852, 868
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 654, 891
Cac8I GCNNGC 6 cut(s) 130, 172, 366, 448, 619, 830
CaiI CAGNNNCTG 1 cut(s) 401
CfoI GCGC 3 cut(s) 77, 364, 419
Cfr13I GGNCC 3 cut(s) 145, 381, 883
CseI GACGC 2 cut(s) 292, 398
Csp6I GTAC 1 cut(s) 248
CviAII CATG 3 cut(s) 70, 316, 496
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 3 cut(s) 36, 100, 754
DinI GGCGCC 1 cut(s) 418
DpnI GATC 3 cut(s) 56, 670, 704
DpnII GATC 3 cut(s) 54, 668, 702
DraI TTTAAA 1 cut(s) 768
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 532
Eco105I TACGTA 1 cut(s) 510
Eco130I CCWWGG 3 cut(s) 141, 163, 336
Eco147I AGGCCT 2 cut(s) 288, 721
Eco32I GATATC 2 cut(s) 351, 493
Eco57I CTGAAG 3 cut(s) 213, 700, 863
Eco72I CACGTG 1 cut(s) 344
EcoO109I RGGNCCY 2 cut(s) 145, 883
EcoRII CCWGG 3 cut(s) 419, 721, 811
EcoRV GATATC 2 cut(s) 351, 493
EcoT14I CCWWGG 3 cut(s) 141, 163, 336
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 141, 163, 336
FaeI CATG 3 cut(s) 73, 319, 499
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 2 cut(s) 246, 725
FatI CATG 3 cut(s) 69, 315, 495
FauI CCCGC 2 cut(s) 50, 179
Fnu4HI GCNGC 5 cut(s) 360, 532, 541, 663, 819
FokI GGATG 4 cut(s) 263, 772, 859, 875
Fsp4HI GCNGC 5 cut(s) 360, 532, 541, 663, 819
FspBI CTAG 1 cut(s) 176
GlaI GCGC 3 cut(s) 76, 363, 418
GluI GCNGC 5 cut(s) 360, 532, 541, 663, 819
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 2 cut(s) 78, 420
HaeIII GGCC 8 cut(s) 66, 146, 288, 383, 534, 721, 876, 885
HgaI GACGC 2 cut(s) 292, 398
HhaI GCGC 3 cut(s) 77, 364, 419
Hin1I GRCGYC 3 cut(s) 303, 390, 417
Hin1II CATG 3 cut(s) 73, 319, 499
Hin6I GCGC 3 cut(s) 75, 362, 417
HinP1I GCGC 3 cut(s) 75, 362, 417
HincII GTYRAC 2 cut(s) 388, 477
HindII GTYRAC 2 cut(s) 388, 477
HinfI GANTC 1 cut(s) 462
HpaI GTTAAC 1 cut(s) 477
HphI GGTGA 5 cut(s) 109, 214, 464, 530, 583
Hpy166II GTNNAC 4 cut(s) 313, 388, 477, 840
Hpy188I TCNGA 3 cut(s) 39, 193, 922
Hpy188III TCNNGA 1 cut(s) 706
Hpy8I GTNNAC 4 cut(s) 313, 388, 477, 840
Hpy99I CGWCG 1 cut(s) 676
HpyAV CCTTC 6 cut(s) 147, 198, 419, 587, 742, 887
HpyCH4III ACNGT 3 cut(s) 310, 837, 895
HpyCH4IV ACGT 3 cut(s) 343, 509, 674
HpyCH4V TGCA 6 cut(s) 446, 543, 608, 821, 828, 866
HpyF3I CTNAG 3 cut(s) 36, 100, 754
HpySE526I ACGT 3 cut(s) 343, 509, 674
Hsp92I GRCGYC 3 cut(s) 303, 390, 417
Hsp92II CATG 3 cut(s) 73, 319, 499
HspAI GCGC 3 cut(s) 75, 362, 417
KasI GGCGCC 1 cut(s) 416
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 3 cut(s) 54, 668, 702
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 3 cut(s) 346, 527, 805
LweI GCATC 3 cut(s) 285, 445, 853
MaeI CTAG 1 cut(s) 176
MaeII ACGT 3 cut(s) 343, 509, 674
MaeIII GTNAC 3 cut(s) 107, 304, 505
MalI GATC 3 cut(s) 56, 670, 704
MboI GATC 3 cut(s) 54, 668, 702
MboII GAAGA 2 cut(s) 211, 706
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 931
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 1 cut(s) 471
MnlI CCTC 9 cut(s) 77, 104, 125, 259, 560, 647, 650, 702, 772
MseI TTAA 2 cut(s) 476, 767
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 2 cut(s) 104, 359
MspR9I CCNGG 3 cut(s) 421, 723, 813
MvaI CCWGG 3 cut(s) 421, 723, 813
MvnI CGCG 1 cut(s) 364
NarI GGCGCC 1 cut(s) 417
NdeII GATC 3 cut(s) 54, 668, 702
NlaIII CATG 3 cut(s) 73, 319, 499
NlaIV GGNNCC 2 cut(s) 169, 418
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 2 cut(s) 107, 304
OliI CACNNNNGTG 1 cut(s) 227
PceI AGGCCT 2 cut(s) 288, 721
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 5 cut(s) 361, 533, 542, 664, 820
PleI GAGTC 1 cut(s) 470
PluTI GGCGCC 1 cut(s) 420
PmaCI CACGTG 1 cut(s) 344
PmlI CACGTG 1 cut(s) 344
PpsI GAGTC 1 cut(s) 470
Ppu21I YACGTR 2 cut(s) 344, 510
Psp6I CCWGG 3 cut(s) 419, 721, 811
PspCI CACGTG 1 cut(s) 344
PspGI CCWGG 3 cut(s) 419, 721, 811
PspN4I GGNNCC 2 cut(s) 169, 418
PspPI GGNCC 3 cut(s) 145, 381, 883
PstNI CAGNNNCTG 1 cut(s) 401
PvuII CAGCTG 2 cut(s) 104, 359
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 2 cut(s) 476, 767
SatI GCNGC 5 cut(s) 360, 532, 541, 663, 819
Sau3AI GATC 3 cut(s) 54, 668, 702
Sau96I GGNCC 3 cut(s) 145, 381, 883
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 3 cut(s) 421, 723, 813
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 285, 445, 853
SfiI GGCCNNNNNGGCC 1 cut(s) 882
SfoI GGCGCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 227
SnaBI TACGTA 1 cut(s) 510
Sse9I AATT 1 cut(s) 931
SseBI AGGCCT 2 cut(s) 288, 721
SsiI CCGC 5 cut(s) 43, 172, 450, 531, 662
SspDI GGCGCC 1 cut(s) 416
SspI AATATT 2 cut(s) 441, 772
SspMI CTAG 1 cut(s) 176
StuI AGGCCT 2 cut(s) 288, 721
StyD4I CCNGG 3 cut(s) 419, 721, 811
StyI CCWWGG 3 cut(s) 141, 163, 336
TaaI ACNGT 3 cut(s) 310, 837, 895
TaiI ACGT 3 cut(s) 346, 512, 677
TaqI TCGA 3 cut(s) 671, 705, 927
TasI AATT 1 cut(s) 931
TauI GCSGC 2 cut(s) 534, 665
Tru1I TTAA 2 cut(s) 476, 767
Tru9I TTAA 2 cut(s) 476, 767
TscAI CASTG 2 cut(s) 654, 898
TseFI GTSAC 2 cut(s) 107, 304
TseI GCWGC 3 cut(s) 359, 540, 818
Tsp45I GTSAC 2 cut(s) 107, 304
TspDTI ATGAA 1 cut(s) 807
TspRI CASTG 2 cut(s) 654, 898
Van91I CCANNNNNTGG 1 cut(s) 401
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.