Rh7BG336500

3-hydroxyisobutyrate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004641
Physical Location & Seq
Forward (+)
35076163 .. 35077107
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG336500.1

Sequence Viewer

Length: 945 bp
ATGGGAACTCCTTACCCAAACCCCATAACTCCTTCTCAGACCCGCATTGGCTGGATCGGCATTGGCCTCATGGGCGCTCCTATGGCCTCTCGCCTCATCTCAGCCGGCTACTCCCTCACTATCTATGCTCGCACTCCTTCTAAAGCTCTTTCACTACAATCCCAAGGTGCCCTCCTAGCCCAATCCCCTTTCGAAGTTGCTCAACGTAGTGATGTTGTCTTCACCATAGTGGGACACCCACCAGATGTACGACAAGTTATCTTGGAGGCAAACGGCATCCTTTCAGGCCTAAACCCGAACGGCGTCACGGTGGACATGACCACCAATCTACCAGCCTCAGCACGTGAGATATCATCAGCTGTGCGTGCGAAGGATTGTTGGGCAGTTGACGCCCCAGTCTCCGGGGCAGATGTTGGCGCAAGAGAAGGGAAACTTGGAATATTTGCTGGCGGTGATGCTGGAGTCGTCGAGTGGTTAACACCATTGTTTGATATCATGGGGAAGGTTACGTATATGGGTGAAGCAGGGTGCGGCCAGAGTTGCAAGATAGCGAATAACATTGTGGGAGGGGTGAGTTTTGTCGGGTTGAGTGAAGGTTTGGTGTTTGCAGAGAGAGCTGGCTTGGATTTGAAGCAGTTTTTGGAAGCAGTGAGAGGAGGGGCGGCAGGATCGACGTTGATGGAGTTATTTGGGGAGAAAATGATCGAGAGGGACTTCAGGCCTGGTGGGTTTGTTGAGTATATTGTGAAGGACTTAGGGATGGGATTAAATATTGTGGAGGAAACTAATGATGAAAGGGTGGTGGTGTTGCCTGGTGCTGCATTGTGCAAGCAACTGTTTACTGGGATGGTTGCTAATGGGGATGCAAAGCTCGGCCTTCAGGGCCTTATCACTGTTATACAAAGGCTCAATGGCATATCTGAAATCGACAATTCTACTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

314

Amino Acids

32.92

Weight (kDa)

5.01

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 15 - 173 2.2e-44 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 15 - 90 3.7e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 176 - 299 1.6e-24 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 167
AciI CCGC 4 cut(s) 43, 450, 531, 662
AclWI GGATC 2 cut(s) 62, 676
AcoI YGGCCR 1 cut(s) 532
AcuI CTGAAG 2 cut(s) 700, 863
AcvI CACGTG 1 cut(s) 344
AcyI GRCGYC 2 cut(s) 303, 390
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 1 cut(s) 631
AjnI CCWGG 2 cut(s) 721, 811
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 623, 655
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 4 cut(s) 146, 359, 617, 871
AluI AGCT 4 cut(s) 146, 359, 617, 871
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 2 cut(s) 62, 676
AoxI GGCC 7 cut(s) 64, 84, 286, 532, 719, 874, 883
ApeKI GCWGC 1 cut(s) 818
AspLEI GCGC 2 cut(s) 77, 419
AspS9I GGNCC 1 cut(s) 883
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 4 cut(s) 214, 464, 530, 583
AsuII TTCGAA 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 167
BbrPI CACGTG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 211
BbvCI CCTCAGC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 805
BccI CCATC 3 cut(s) 673, 754, 841
BceAI ACGGC 2 cut(s) 289, 316
BciT130I CCWGG 2 cut(s) 723, 813
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 176
BfoI RGCGCY 1 cut(s) 78
BglI GCCNNNNNGGC 2 cut(s) 72, 882
BisI GCNGC 3 cut(s) 532, 663, 819
BlsI GCNGC 3 cut(s) 533, 664, 820
Bme1390I CCNGG 3 cut(s) 403, 723, 813
BmgT120I GGNCC 1 cut(s) 883
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 3 cut(s) 403, 723, 813
BmrI ACTGGG 2 cut(s) 389, 852
BmsI GCATC 3 cut(s) 285, 445, 853
BmuI ACTGGG 2 cut(s) 389, 852
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 403
BsaAI YACGTR 2 cut(s) 344, 510
BsaHI GRCGYC 2 cut(s) 303, 390
BsaJI CCNNGG 2 cut(s) 163, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 395, 847
BseBI CCWGG 2 cut(s) 723, 813
BseDI CCNNGG 2 cut(s) 163, 402
BseGI GGATG 4 cut(s) 276, 765, 852, 868
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 3 cut(s) 50, 114, 351
BseNI ACTGG 2 cut(s) 395, 847
BseRI GAGGAG 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 1 cut(s) 805
BshFI GGCC 7 cut(s) 66, 86, 288, 534, 721, 876, 885
BshNI GGYRCC 1 cut(s) 167
BsiSI CCGG 2 cut(s) 105, 402
BslFI GGGAC 2 cut(s) 246, 725
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 403
BsmFI GGGAC 2 cut(s) 246, 725
BsnI GGCC 7 cut(s) 66, 86, 288, 534, 721, 876, 885
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 54, 668, 702
BspACI CCGC 4 cut(s) 43, 450, 531, 662
BspANI GGCC 7 cut(s) 66, 86, 288, 534, 721, 876, 885
BspCNI CTCAG 3 cut(s) 49, 113, 350
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 2 cut(s) 62, 676
BspT104I TTCGAA 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 395, 847
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 163, 402
BssMI GATC 3 cut(s) 54, 668, 702
BssNI GRCGYC 2 cut(s) 303, 390
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 723, 813
Bst4CI ACNGT 3 cut(s) 310, 837, 895
BstACI GRCGYC 2 cut(s) 303, 390
BstBAI YACGTR 2 cut(s) 344, 510
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 6 cut(s) 106, 130, 366, 448, 619, 830
BstDEI CTNAG 4 cut(s) 36, 100, 337, 754
BstF5I GGATG 4 cut(s) 276, 765, 852, 868
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 419
BstKTI GATC 3 cut(s) 57, 671, 705
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 54, 668, 702
BstMWI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 389, 540, 614, 882
BstNI CCWGG 2 cut(s) 723, 813
BstSCI CCNGG 3 cut(s) 401, 721, 811
BstSLI GKGCMC 1 cut(s) 172
BstSNI TACGTA 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 805
BstV2I GAAGAC 1 cut(s) 211
BsuRI GGCC 7 cut(s) 66, 86, 288, 534, 721, 876, 885
BtsCI GGATG 4 cut(s) 276, 765, 852, 868
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 654, 891
Cac8I GCNNGC 6 cut(s) 106, 130, 366, 448, 619, 830
CfoI GCGC 2 cut(s) 77, 419
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 883
CseI GACGC 2 cut(s) 292, 398
Csp6I GTAC 1 cut(s) 248
CviAII CATG 3 cut(s) 70, 316, 496
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 4 cut(s) 36, 100, 337, 754
DpnI GATC 3 cut(s) 56, 670, 704
DpnII GATC 3 cut(s) 54, 668, 702
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 532
Eco105I TACGTA 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 163
Eco147I AGGCCT 2 cut(s) 288, 721
Eco32I GATATC 2 cut(s) 351, 493
Eco57I CTGAAG 2 cut(s) 700, 863
Eco72I CACGTG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 883
EcoRII CCWGG 2 cut(s) 721, 811
EcoRV GATATC 2 cut(s) 351, 493
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 3 cut(s) 73, 319, 499
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 2 cut(s) 246, 725
FatI CATG 3 cut(s) 69, 315, 495
FauI CCCGC 1 cut(s) 50
Fnu4HI GCNGC 3 cut(s) 532, 663, 819
FokI GGATG 4 cut(s) 263, 772, 859, 875
Fsp4HI GCNGC 3 cut(s) 532, 663, 819
FspBI CTAG 1 cut(s) 176
GlaI GCGC 2 cut(s) 76, 418
GluI GCNGC 3 cut(s) 532, 663, 819
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 7 cut(s) 66, 86, 288, 534, 721, 876, 885
HapII CCGG 2 cut(s) 105, 402
HgaI GACGC 2 cut(s) 292, 398
HhaI GCGC 2 cut(s) 77, 419
Hin1I GRCGYC 2 cut(s) 303, 390
Hin1II CATG 3 cut(s) 73, 319, 499
Hin6I GCGC 2 cut(s) 75, 417
HinP1I GCGC 2 cut(s) 75, 417
HincII GTYRAC 2 cut(s) 388, 477
HindII GTYRAC 2 cut(s) 388, 477
HinfI GANTC 1 cut(s) 462
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 2 cut(s) 105, 402
HphI GGTGA 4 cut(s) 214, 464, 530, 583
Hpy166II GTNNAC 4 cut(s) 313, 388, 477, 840
Hpy188I TCNGA 2 cut(s) 39, 922
Hpy188III TCNNGA 1 cut(s) 706
Hpy8I GTNNAC 4 cut(s) 313, 388, 477, 840
Hpy99I CGWCG 2 cut(s) 470, 676
HpyAV CCTTC 8 cut(s) 42, 147, 364, 419, 496, 587, 742, 887
HpyCH4III ACNGT 3 cut(s) 310, 837, 895
HpyCH4IV ACGT 4 cut(s) 205, 343, 509, 674
HpyCH4V TGCA 5 cut(s) 543, 608, 821, 828, 866
HpyF10VI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 389, 540, 614, 882
HpyF3I CTNAG 4 cut(s) 36, 100, 337, 754
HpySE526I ACGT 4 cut(s) 205, 343, 509, 674
Hsp92I GRCGYC 2 cut(s) 303, 390
Hsp92II CATG 3 cut(s) 73, 319, 499
HspAI GCGC 2 cut(s) 75, 417
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 3 cut(s) 54, 668, 702
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 1 cut(s) 805
LweI GCATC 3 cut(s) 285, 445, 853
MaeI CTAG 1 cut(s) 176
MaeII ACGT 4 cut(s) 205, 343, 509, 674
MaeIII GTNAC 2 cut(s) 304, 505
MalI GATC 3 cut(s) 56, 670, 704
MboI GATC 3 cut(s) 54, 668, 702
MboII GAAGA 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 931
MlyI GAGTC 1 cut(s) 471
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 2 cut(s) 476, 767
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 2 cut(s) 105, 402
MspR9I CCNGG 3 cut(s) 403, 723, 813
MvaI CCWGG 2 cut(s) 723, 813
MwoI GCNNNNNNNGC 9 cut(s) 57, 72, 83, 176, 365, 389, 540, 614, 882
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 1 cut(s) 403
NdeII GATC 3 cut(s) 54, 668, 702
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 3 cut(s) 73, 319, 499
NlaIV GGNNCC 1 cut(s) 169
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 1 cut(s) 304
NspV TTCGAA 1 cut(s) 192
OliI CACNNNNGTG 1 cut(s) 227
PceI AGGCCT 2 cut(s) 288, 721
PdiI GCCGGC 1 cut(s) 106
PkrI GCNGC 3 cut(s) 533, 664, 820
PleI GAGTC 1 cut(s) 470
PmaCI CACGTG 1 cut(s) 344
PmlI CACGTG 1 cut(s) 344
PpsI GAGTC 1 cut(s) 470
Ppu21I YACGTR 2 cut(s) 344, 510
Psp6I CCWGG 2 cut(s) 721, 811
PspCI CACGTG 1 cut(s) 344
PspGI CCWGG 2 cut(s) 721, 811
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 883
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 2 cut(s) 476, 767
SatI GCNGC 3 cut(s) 532, 663, 819
Sau3AI GATC 3 cut(s) 54, 668, 702
Sau96I GGNCC 1 cut(s) 883
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 3 cut(s) 403, 723, 813
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 285, 445, 853
SfiI GGCCNNNNNGGCC 1 cut(s) 882
SfuI TTCGAA 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 227
SnaBI TACGTA 1 cut(s) 510
Sse9I AATT 1 cut(s) 931
SseBI AGGCCT 2 cut(s) 288, 721
SsiI CCGC 4 cut(s) 43, 450, 531, 662
SspI AATATT 2 cut(s) 441, 772
SspMI CTAG 1 cut(s) 176
StuI AGGCCT 2 cut(s) 288, 721
StyD4I CCNGG 3 cut(s) 401, 721, 811
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 3 cut(s) 310, 837, 895
TaiI ACGT 4 cut(s) 208, 346, 512, 677
TaqI TCGA 5 cut(s) 192, 468, 671, 705, 927
TasI AATT 1 cut(s) 931
TauI GCSGC 2 cut(s) 534, 665
Tru1I TTAA 2 cut(s) 476, 767
Tru9I TTAA 2 cut(s) 476, 767
TscAI CASTG 2 cut(s) 654, 898
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 818
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 807
TspRI CASTG 2 cut(s) 654, 898
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.