AT1G71180

Contains the following InterPro domains 6-phosphogluconate dehydrogenase, NAD-binding (InterPro IPR006115), Dehydrogenase, multihelical (InterPro IPR013328), 6-phosphogluconate dehydrogenase, C-terminal-like (InterPro IPR008927), 3-hydroxyacid dehydrogenase reductase (InterPro IPR015815), NAD(P)-binding domain (InterPro IPR016040)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004641
Physical Location & Seq
Forward (+)
26832267 .. 26833709
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G71180.1

Sequence Viewer

Length: 957 bp
ATGTTCTACCAATCGTCGTCGTCTCTCACGCAAATTCTTCATTCGTTCTGTGGAAACACAGAAATGGAGACTCCATATCCGAAACTCATCGATCCTTCAAAGACTCGAATAGGATGGATTGGCATCGGGATAATGGGATCGGCCATGGTTTCTCATATTATCGCCGCAGGCTATTCTGTCACAGTTTACGCTCGCGACCTCCGGAAAACAAAAGATCTGCAAACCAAAGGAGCTCGCATAGCCAATAGCCCCAAAGAGTTAGCGGAGATGAGCGACGTTGTGTTCACGATTGTTGGAAACTTCAACGATGTCCGATCATTGCTTCTCGGTGACGATGGTGTACTCTCTGGTCTTACGCCCGGTGGAGTAACAGTGGACATGACTAGTAGCAAACCAGGATTGGCGCGTGAAATACACGCAGAAGCGAGGCGTAGGAATTGTTGGGCGGTGGATGCGCCGGTATCAGGTGGAGACGCTGGAGCACGAGAGGGGACGTTAGGGATTTTCGCCGGCGGAGATTCAGAGATTGTGGAGTGGTTGTCCCCGGTGATGAAGAATATAGGGACTGTTACTTATATGGGAGAAGCAGGGAGTGGGCAAAGCTGCAAGATAGGGAATCAGATTGCTGGTGCGAGCAACCTGGTTGGACTTGCTGAAGGGATAGTGTTCGCTGAGAAAGCGGGTTTAGATACAGTGAAATGGCTCGAGGCGGTGAAGGATGGTGCAGCTGGATCGGCGGTTATGCGGCTGTTTGGGGAGATGATTGTGAAGAGGGATTATAGGGCGACTGGCTTTGCGGAGTATATGGTGAAAGATTTGGGAATGGCTGCAGAGGCGGCGATGCCTGGTGCTGCTCTGAGCAAGCAGTTGTTCACTGGAATGGTGGCTAATGGAGATGGCAAGTTAGGTATTCAAGGCGTCGTCTCTGTTATTAGAAGACTTAACGGCATATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

318

Amino Acids

33.38

Weight (kDa)

6.34

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 36 - 194 1.5e-47 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 36 - 98 6.8e-08 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 197 - 310 8.1e-20 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 195, 406
AccIII TCCGGA 1 cut(s) 201
AclWI GGATC 3 cut(s) 86, 145, 739
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 99, 304, 914
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 3 cut(s) 394, 639, 844
AluBI AGCT 3 cut(s) 233, 603, 728
AluI AGCT 3 cut(s) 233, 603, 728
Alw21I GWGCWC 2 cut(s) 235, 484
Alw26I GTCTC 4 cut(s) 27, 62, 465, 928
AlwI GGATC 3 cut(s) 86, 145, 739
Ama87I CYCGRG 1 cut(s) 704
Aor13HI TCCGGA 1 cut(s) 201
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 4 cut(s) 603, 725, 827, 851
ApoI RAATTY 1 cut(s) 33
AspLEI GCGC 2 cut(s) 406, 457
AsuC2I CCSGG 2 cut(s) 360, 545
AsuHPI GGTGA 4 cut(s) 341, 559, 724, 820
AvaI CYCGRG 1 cut(s) 704
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 943
Bbv12I GWGCWC 2 cut(s) 235, 484
BbvI GCAGC 4 cut(s) 590, 737, 814, 838
BccI CCATC 4 cut(s) 108, 329, 713, 890
BcgI CGANNNNNNTGC 2 cut(s) 714, 748
BciT130I CCWGG 3 cut(s) 396, 641, 846
BcnI CCSGG 2 cut(s) 360, 545
BcoDI GTCTC 4 cut(s) 27, 62, 465, 928
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 828
BglII AGATCT 1 cut(s) 214
BisI GCNGC 7 cut(s) 165, 604, 726, 746, 828, 837, 852
BlsI GCNGC 7 cut(s) 166, 605, 727, 747, 829, 838, 853
Bme1390I CCNGG 5 cut(s) 360, 396, 545, 641, 846
BmeT110I CYCGRG 1 cut(s) 704
BmrFI CCNGG 5 cut(s) 360, 396, 545, 641, 846
BmsI GCATC 3 cut(s) 132, 442, 831
BpiI GAAGAC 1 cut(s) 943
BpmI CTGGAG 1 cut(s) 498
BpuMI CCSGG 2 cut(s) 360, 545
Bsa29I ATCGAT 1 cut(s) 90
BsaBI GATNNNNATC 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 2 cut(s) 144, 543
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 524, 554
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 2 cut(s) 457, 509
Bse1I ACTGG 2 cut(s) 793, 880
Bse3DI GCAATG 1 cut(s) 317
Bse8I GATNNNNATC 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 201
BseBI CCWGG 3 cut(s) 396, 641, 846
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 144, 543
BseGI GGATG 3 cut(s) 119, 457, 724
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 2 cut(s) 663, 848
BseNI ACTGG 2 cut(s) 793, 880
BseXI GCAGC 4 cut(s) 590, 737, 814, 838
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 2 cut(s) 195, 406
BshFI GGCC 1 cut(s) 143
BshVI ATCGAT 1 cut(s) 90
BsiHKAI GWGCWC 2 cut(s) 235, 484
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 5 cut(s) 202, 360, 458, 510, 545
BslFI GGGAC 3 cut(s) 505, 526, 577
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 4 cut(s) 27, 62, 465, 928
BsmBI CGTCTC 3 cut(s) 27, 465, 928
BsmFI GGGAC 3 cut(s) 505, 526, 577
BsnI GGCC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 235, 484
Bsp13I TCCGGA 1 cut(s) 201
Bsp143I GATC 5 cut(s) 91, 137, 214, 314, 731
Bsp19I CCATGG 1 cut(s) 144
Bsp68I TCGCGA 1 cut(s) 195
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 2 cut(s) 664, 849
BspDI ATCGAT 1 cut(s) 90
BspEI TCCGGA 1 cut(s) 201
BspFNI CGCG 2 cut(s) 195, 406
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 3 cut(s) 86, 145, 739
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 2 cut(s) 457, 509
BsrI ACTGG 2 cut(s) 793, 880
BssAI RCCGGY 2 cut(s) 457, 509
BssECI CCNNGG 2 cut(s) 144, 543
BssMI GATC 5 cut(s) 91, 137, 214, 314, 731
BssNI GRCGYC 1 cut(s) 918
BssSI CACGAG 1 cut(s) 483
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 483
Bst2UI CCWGG 3 cut(s) 396, 641, 846
Bst4CI ACNGT 4 cut(s) 184, 373, 568, 694
Bst6I CTCTTC 1 cut(s) 764
BstACI GRCGYC 1 cut(s) 918
BstC8I GCNNGC 6 cut(s) 169, 193, 235, 511, 634, 863
BstDEI CTNAG 2 cut(s) 672, 857
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 3 cut(s) 119, 457, 724
BstFNI CGCG 2 cut(s) 195, 406
BstHHI GCGC 2 cut(s) 406, 457
BstKTI GATC 5 cut(s) 94, 140, 217, 317, 734
BstMAI GTCTC 4 cut(s) 27, 62, 465, 928
BstMBI GATC 5 cut(s) 91, 137, 214, 314, 731
BstMWI GCNNNNNNNGC 6 cut(s) 239, 452, 677, 734, 833, 836
BstNI CCWGG 3 cut(s) 396, 641, 846
BstSCI CCNGG 5 cut(s) 358, 394, 543, 639, 844
BstSFI CTRYAG 1 cut(s) 828
BstUI CGCG 2 cut(s) 195, 406
BstV1I GCAGC 4 cut(s) 590, 737, 814, 838
BstV2I GAAGAC 1 cut(s) 943
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 90
BsuRI GGCC 1 cut(s) 143
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 854
BtsCI GGATG 3 cut(s) 119, 457, 724
BtsIMutI CAGTG 3 cut(s) 378, 699, 873
BtuMI TCGCGA 1 cut(s) 195
Cac8I GCNNGC 6 cut(s) 169, 193, 235, 511, 634, 863
CfoI GCGC 2 cut(s) 406, 457
Cfr10I RCCGGY 2 cut(s) 457, 509
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 2 cut(s) 482, 907
CsiI ACCWGGT 1 cut(s) 639
Csp6I GTAC 1 cut(s) 341
CviAII CATG 2 cut(s) 145, 379
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 2 cut(s) 672, 857
DpnI GATC 5 cut(s) 93, 139, 216, 316, 733
DpnII GATC 5 cut(s) 91, 137, 214, 314, 731
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
EciI GGCGGA 1 cut(s) 528
Ecl136II GAGCTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 704
EcoICRI GAGCTC 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 394, 639, 844
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 144
Esp3I CGTCTC 3 cut(s) 27, 465, 928
FaeI CATG 2 cut(s) 148, 382
FaqI GGGAC 3 cut(s) 505, 526, 577
FatI CATG 2 cut(s) 144, 378
FauI CCCGC 1 cut(s) 673
Fnu4HI GCNGC 7 cut(s) 165, 604, 726, 746, 828, 837, 852
FokI GGATG 3 cut(s) 126, 464, 731
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 7 cut(s) 165, 604, 726, 746, 828, 837, 852
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 405, 456
GluI GCNGC 7 cut(s) 165, 604, 726, 746, 828, 837, 852
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 1 cut(s) 143
HapII CCGG 5 cut(s) 202, 360, 458, 510, 545
HgaI GACGC 2 cut(s) 482, 907
HhaI GCGC 2 cut(s) 406, 457
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 2 cut(s) 148, 382
Hin6I GCGC 2 cut(s) 404, 455
HinP1I GCGC 2 cut(s) 404, 455
HinfI GANTC 4 cut(s) 70, 103, 518, 616
HpaII CCGG 5 cut(s) 202, 360, 458, 510, 545
HphI GGTGA 4 cut(s) 341, 559, 724, 820
Hpy166II GTNNAC 5 cut(s) 187, 285, 341, 376, 873
Hpy188I TCNGA 5 cut(s) 81, 314, 523, 621, 858
Hpy188III TCNNGA 5 cut(s) 127, 194, 202, 286, 954
Hpy8I GTNNAC 5 cut(s) 187, 285, 341, 376, 873
Hpy99I CGWCG 4 cut(s) 19, 22, 278, 923
HpyAV CCTTC 3 cut(s) 105, 650, 709
HpyCH4III ACNGT 4 cut(s) 184, 373, 568, 694
HpyCH4IV ACGT 2 cut(s) 276, 494
HpyCH4V TGCA 4 cut(s) 220, 606, 725, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 452, 677, 734, 833, 836
HpyF3I CTNAG 2 cut(s) 672, 857
HpySE526I ACGT 2 cut(s) 276, 494
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 2 cut(s) 148, 382
HspAI GCGC 2 cut(s) 404, 455
Kpn2I TCCGGA 1 cut(s) 201
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
Kzo9I GATC 5 cut(s) 91, 137, 214, 314, 731
LmnI GCTCC 2 cut(s) 230, 479
Lsp1109I GCAGC 4 cut(s) 590, 737, 814, 838
LweI GCATC 3 cut(s) 132, 442, 831
MabI ACCWGGT 1 cut(s) 639
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 276, 494
MaeIII GTNAC 4 cut(s) 178, 329, 367, 568
MalI GATC 5 cut(s) 93, 139, 216, 316, 733
MboI GATC 5 cut(s) 91, 137, 214, 314, 731
MboII GAAGA 4 cut(s) 29, 565, 781, 948
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 235, 484
MluCI AATT 2 cut(s) 33, 436
MlyI GAGTC 2 cut(s) 64, 97
MmeI TCCRAC 2 cut(s) 274, 625
MnlI CCTC 6 cut(s) 209, 420, 481, 700, 765, 826
MreI CGCCGGCG 1 cut(s) 509
MroI TCCGGA 1 cut(s) 201
MroNI GCCGGC 1 cut(s) 509
MseI TTAA 1 cut(s) 942
MslI CAYNNNNRTG 2 cut(s) 62, 878
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 5 cut(s) 202, 360, 458, 510, 545
MspR9I CCNGG 5 cut(s) 360, 396, 545, 641, 846
MvaI CCWGG 3 cut(s) 396, 641, 846
MvnI CGCG 2 cut(s) 195, 406
MwoI GCNNNNNNNGC 6 cut(s) 239, 452, 677, 734, 833, 836
NaeI GCCGGC 1 cut(s) 511
NciI CCSGG 2 cut(s) 360, 545
NcoI CCATGG 1 cut(s) 144
NdeII GATC 5 cut(s) 91, 137, 214, 314, 731
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 2 cut(s) 148, 382
NmuCI GTSAC 2 cut(s) 178, 329
NruI TCGCGA 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 704
PdiI GCCGGC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 518, 616
PkrI GCNGC 7 cut(s) 166, 605, 727, 747, 829, 838, 853
PleI GAGTC 2 cut(s) 64, 97
PpsI GAGTC 2 cut(s) 64, 97
Psp124BI GAGCTC 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 394, 639, 844
PspGI CCWGG 3 cut(s) 394, 639, 844
PspXI VCTCGAGB 1 cut(s) 704
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 728
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 2 cut(s) 62, 878
SacI GAGCTC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 942
SatI GCNGC 7 cut(s) 165, 604, 726, 746, 828, 837, 852
Sau3AI GATC 5 cut(s) 91, 137, 214, 314, 731
SchI GAGTC 2 cut(s) 64, 97
ScrFI CCNGG 5 cut(s) 360, 396, 545, 641, 846
SduI GDGCHC 2 cut(s) 235, 484
SetI ASST 9 cut(s) 201, 235, 279, 469, 497, 605, 642, 730, 910
SexAI ACCWGGT 1 cut(s) 639
SfaNI GCATC 3 cut(s) 132, 442, 831
SfcI CTRYAG 1 cut(s) 828
Sfr274I CTCGAG 1 cut(s) 704
SgrAI CRCCGGYG 1 cut(s) 509
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 62, 878
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 2 cut(s) 33, 436
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 1 cut(s) 235
StyD4I CCNGG 5 cut(s) 358, 394, 543, 639, 844
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 184, 373, 568, 694
TaiI ACGT 2 cut(s) 279, 497
TaqI TCGA 3 cut(s) 90, 106, 705
TasI AATT 2 cut(s) 33, 436
TatI WGTACW 1 cut(s) 340
TauI GCSGC 3 cut(s) 167, 748, 839
TfiI GAWTC 2 cut(s) 518, 616
Tru1I TTAA 1 cut(s) 942
Tru9I TTAA 1 cut(s) 942
TscAI CASTG 3 cut(s) 378, 699, 880
TseFI GTSAC 2 cut(s) 178, 329
TseI GCWGC 4 cut(s) 603, 725, 827, 851
Tsp45I GTSAC 2 cut(s) 178, 329
TspDTI ATGAA 2 cut(s) 29, 566
TspRI CASTG 3 cut(s) 378, 699, 880
XapI RAATTY 1 cut(s) 33
XhoI CTCGAG 1 cut(s) 704
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.