Rmu_sc0007429.1_g000004

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007429.1
OG0006671
Physical Location & Seq
Forward (+)
10188 .. 14691
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2676 bp
atgcaatatttccaaaccctgaaaagaagcaagctcatcattaacaccagagaagcagagcagagctgcttggctctactccaactgtgcgacgtcgttccgaagctgacccaaatccaagcccacattgtcaaattgggcctccgaaacaaccccttagtgctcaccaagttcgcctccaccgcctccgaccacaaggccattgactacgcctcttccttcctgttctcgccggattccgatacccatttattcgatgcgtttctcttcaacaccgtcatcagggcttacgctcagaccggtcatgcaaagcataaagctttgtgcttttacagagttatggttaaaggtgaagtcttgcctaataaattcacctacccttttgttctcaaggcctgtgccggcgtcggggacttgaatttggggaagtcggttcatgggtcggtggtgaaattcgggtttggtgatgatgtccatgttcggaacaccatggttcatatgtactgttgctgcggtggagggattgaaagtgcacggaaggtgttcgatgaaatgcctaactgggactctgtttcgtggagcgcgatgattggaggatatgtgagggtggggtggtctagtgatgcggtggagttgtttagggagatgcagatggcgggagtgaggccggacgagattactatggtttcggttttgtctgcgtgtaccgatttgggtgcgcttgagcttggcaagtgggtggagtcctatattgagaaagaggggattcagaaaagtgtggagctttgtaatgcgctcattgacatgtttgcgaaatgtggcaatgttgataaggctttgaagttgtttaggaacatgagcgggagaacaattgtttcttggacttctgtgattgatggtttggcaatgcatggccgcggagaagaggccgtttctttgtttgaggaaatgattggagtcggggtggaaccggatgatgttgcgtttataggactgctttcggcttgtagtcattccgggttggtcgataagggaaagaggtacttcagttcaatgatggaaaaatttggacgtgtacctaagatcgaacactatgggtgcatggtggatatgttatgccgagctggacatgtcaaggaggcattggagtttattcggaaaatgcctattcagccaaatccaatagttttgcgaaccctgatcagtgcttgccgtgcgcatggtgagctcaagcttggagagagcatcaccaaggagttaatcagggctgaacctatgcatgagtcgaactatgtcctgctttccaacatctatgcaaaaatgaaccattgggagaagaaaaccaagactagagaggcgatggacaagaaagggatgaaaaagatccctgggagcactatgattgagcaagataatgaaatttacgaatttgtggcaggggataaatcgcataaacagtccaaagagatttatgaaatggttgatgaaatggggaggaagatgaagacggctggatatgttcccactacatcagaggttttgctagacattgatgaagaagataaagaagatgctttaaataggcatagtgaaaagttggccattgctttcgccctcctgaacacgccaccaggaactcctattcgcatagtaaagaatctgcgggtttgtgatgattgccactctgctactaagtacatctctaagatttataatcgagaaatagtggtgagggaccgaaacaggtactcggacagtgaattgcaaaaagaaaatgattgcatctttggaaaaccaaggatggtagaggatgtactgactgacttgctgatgcttgaaaatcagctgccattcttcattcttggggacaatccagctacagttaggagtctttccatacagtttcttcatgagcagggtgctatagctttcgggatagagttagatgattctaccagagtagaagcgaaacatattgttgatctctttagagagctattgatagctcctatgtcgaaagaaccacccatgaccaagacagaaaaatctgcaacaacaccttgcattgaaaagctacacctggctggagtccaatttaaacccgtcagaagcacgaaattgtttgagatacgatttgatgtaaccacgaaatggtttggcataggattccataaagggattctgaagattccaaaattgcacacaggggatacaacggagcttgtacttagaaacttaattgcctttgaacaatgcattacttctgaagattattgcataagtgattatgttagcatcatggatgagttggtggacgatccaaaagatgtggggttgcttgttaagtataaaattgttgaaaatgatctcggtggtggcgatcaggatttggctaccttgatcaacaacctttcaactgggattgtgtataagaagtggtcatctttgtggagagctggacaagttctacagttcgaagtggcacaaatggatggcaaatttgagaagcagttatttcaataccccttggacaactatttctttcgtggcagcaattgttctcctcatactcactgccattcaaactatctgctcaattatctctcttacgtgagcgttccttcattctgtcgatcgactctgctatcccatccttttccatcactttggaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

891

Amino Acids

100.5

Weight (kDa)

6.47

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 134 4.6e-07 PPR repeat family
PPR_2 PF13041 189 - 235 4.2e-09 PPR repeat family
PPR PF01535 191 - 221 2.4e-07 PPR repeat
PPR_2 PF13041 290 - 337 8.8e-10 PPR repeat family
PPR PF01535 292 - 322 5.9e-08 PPR repeat
PPR_1 PF12854 362 - 388 5.6e-06 PPR repeat
E_motif PF20431 407 - 469 1.5e-13 E motif
Eplus_motif PF20430 474 - 501 1.7e-07 E+ motif
DYW_deaminase PF14432 505 - 584 1.9e-31 DYW family of nucleic acid deaminases
DUF247 PF03140 585 - 814 5.4e-48 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1713
AatII GACGTC 1 cut(s) 96
Acc16I TGCGCA 1 cut(s) 1218
AccB7I CCANNNNNTGG 1 cut(s) 2151
AccBSI CCGCTC 1 cut(s) 861
AccII CGCG 2 cut(s) 584, 918
AciI CCGC 8 cut(s) 183, 513, 626, 656, 861, 916, 918, 1663
AclWI GGATC 2 cut(s) 1378, 2312
AcoI YGGCCR 2 cut(s) 913, 1599
AcsI RAATTY 7 cut(s) 368, 418, 452, 1064, 1419, 1427, 2498
AcuI CTGAAG 3 cut(s) 1030, 2204, 2286
AcyI GRCGYC 2 cut(s) 93, 405
AfaI GTAC 8 cut(s) 503, 706, 1043, 1077, 1697, 1748, 1815, 2226
AfiI CCNNNNNNNGG 3 cut(s) 282, 408, 2151
AflIII ACRYGT 3 cut(s) 804, 1072, 1129
AgeI ACCGGT 1 cut(s) 299
AjiI CACGTC 1 cut(s) 1073
AjnI CCWGG 3 cut(s) 1387, 1630, 2079
Alw21I GWGCWC 4 cut(s) 165, 535, 1230, 1397
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 2 cut(s) 1378, 2312
AoxI GGCC 7 cut(s) 139, 198, 393, 665, 913, 927, 1599
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 4 cut(s) 66, 510, 1846, 2550
ApoI RAATTY 7 cut(s) 368, 418, 452, 1064, 1419, 1427, 2498
AsiGI ACCGGT 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 542
AspLEI GCGC 4 cut(s) 584, 721, 796, 1219
AspS9I GGNCC 2 cut(s) 139, 1735
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 8 cut(s) 157, 362, 364, 460, 476, 1235, 1240, 1741
AsuII TTCGAA 1 cut(s) 2475
AvaII GGWCC 1 cut(s) 1735
BaeGI GKGCMC 1 cut(s) 535
BaeI ACNNNNGTAYC 2 cut(s) 1033, 1066
BalI TGGCCA 1 cut(s) 1601
BanII GRGCYC 1 cut(s) 1230
BbsI GAAGAC 1 cut(s) 1511
Bbv12I GWGCWC 4 cut(s) 165, 535, 1230, 1397
BbvI GCAGC 4 cut(s) 53, 497, 1833, 2562
BccI CCATC 8 cut(s) 646, 890, 1051, 1354, 1795, 2486, 2658, 2668
BceAI ACGGC 3 cut(s) 914, 1197, 1524
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 3 cut(s) 1389, 1632, 2081
BciVI GTATCC 1 cut(s) 2203
BclI TGATCA 2 cut(s) 1200, 2400
BcnI CCSGG 1 cut(s) 1018
BfaI CTAG 3 cut(s) 618, 1350, 1544
BfmI CTRYAG 3 cut(s) 1878, 1923, 2468
BfuI GTATCC 1 cut(s) 2203
BisI GCNGC 5 cut(s) 67, 511, 916, 1847, 2551
BlsI GCNGC 5 cut(s) 68, 512, 917, 1848, 2552
Bme1390I CCNGG 4 cut(s) 1018, 1389, 1632, 2081
Bme18I GGWCC 1 cut(s) 1735
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 2 cut(s) 139, 1735
BmiI GGNNCC 2 cut(s) 969, 1736
BmrFI CCNGG 4 cut(s) 1018, 1389, 1632, 2081
BmrI ACTGGG 2 cut(s) 571, 2427
BmsI GCATC 8 cut(s) 247, 613, 636, 1254, 1561, 1791, 1821, 2304
BmuI ACTGGG 2 cut(s) 571, 2427
BpiI GAAGAC 1 cut(s) 1511
BpmI CTGGAG 1 cut(s) 2106
Bpu14I TTCGAA 1 cut(s) 2475
BpuEI CTTGAG 3 cut(s) 374, 743, 1214
BpuMI CCSGG 1 cut(s) 1018
BsaAI YACGTR 1 cut(s) 2611
BsaHI GRCGYC 2 cut(s) 93, 405
BsaJI CCNNGG 7 cut(s) 489, 916, 1251, 1387, 1388, 1796, 2526
BsaWI WCCGGW 2 cut(s) 299, 970
BsaXI ACNNNNNCTCC 4 cut(s) 585, 615, 948, 978
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 408, 2151
Bse118I RCCGGY 2 cut(s) 299, 401
Bse1I ACTGG 2 cut(s) 566, 2422
Bse3DI GCAATG 3 cut(s) 829, 912, 1602
BseBI CCWGG 3 cut(s) 1389, 1632, 2081
BseDI CCNNGG 7 cut(s) 489, 916, 1251, 1387, 1388, 1796, 2526
BseGI GGATG 7 cut(s) 979, 1380, 1806, 1816, 2308, 2497, 2650
BseLI CCNNNNNNNGG 3 cut(s) 282, 408, 2151
BseMI GCAATG 3 cut(s) 829, 912, 1602
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 566, 2422
BseRI GAGGAG 1 cut(s) 2553
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 4 cut(s) 53, 497, 1833, 2562
Bsh1236I CGCG 2 cut(s) 584, 918
Bsh1285I CGRYCG 1 cut(s) 2636
BshFI GGCC 7 cut(s) 141, 200, 395, 667, 915, 929, 1601
BshTI ACCGGT 1 cut(s) 299
BsiEI CGRYCG 1 cut(s) 2636
BsiHKAI GWGCWC 4 cut(s) 165, 535, 1230, 1397
BsiSI CCGG 6 cut(s) 233, 300, 402, 668, 971, 1017
BslFI GGGAC 4 cut(s) 425, 578, 1748, 1880
BslI CCNNNNNNNGG 3 cut(s) 282, 408, 2151
BsmFI GGGAC 4 cut(s) 425, 578, 1748, 1880
BsnI GGCC 7 cut(s) 141, 200, 395, 667, 915, 929, 1601
Bsp119I TTCGAA 1 cut(s) 2475
Bsp1286I GDGCHC 4 cut(s) 165, 535, 1230, 1397
Bsp143I GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 8 cut(s) 183, 513, 626, 656, 861, 916, 918, 1663
BspANI GGCC 7 cut(s) 141, 200, 395, 667, 915, 929, 1601
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 2 cut(s) 584, 918
BspHI TCATGA 1 cut(s) 1909
BspLI GGNNCC 2 cut(s) 969, 1736
BspPI GGATC 2 cut(s) 1378, 2312
BspT104I TTCGAA 1 cut(s) 2475
BsrBI CCGCTC 1 cut(s) 861
BsrDI GCAATG 3 cut(s) 829, 912, 1602
BsrFI RCCGGY 2 cut(s) 299, 401
BsrI ACTGG 2 cut(s) 566, 2422
BssAI RCCGGY 2 cut(s) 299, 401
BssECI CCNNGG 7 cut(s) 489, 916, 1251, 1387, 1388, 1796, 2526
BssMI GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
BssNI GRCGYC 2 cut(s) 93, 405
BssT1I CCWWGG 4 cut(s) 489, 1251, 1796, 2526
Bst2UI CCWGG 3 cut(s) 1389, 1632, 2081
Bst4CI ACNGT 8 cut(s) 87, 277, 506, 1458, 1757, 1882, 1902, 2472
Bst6I CTCTTC 3 cut(s) 220, 272, 918
BstACI GRCGYC 2 cut(s) 93, 405
BstBAI YACGTR 1 cut(s) 2611
BstBI TTCGAA 1 cut(s) 2475
BstC8I GCNNGC 3 cut(s) 32, 403, 1210
BstDEI CTNAG 6 cut(s) 157, 294, 1080, 1692, 1704, 2228
BstDSI CCRYGG 2 cut(s) 489, 916
BstF5I GGATG 7 cut(s) 979, 1380, 1806, 1816, 2308, 2497, 2650
BstFNI CGCG 2 cut(s) 584, 918
BstHHI GCGC 4 cut(s) 584, 721, 796, 1219
BstKTI GATC 9 cut(s) 1086, 1203, 1386, 1984, 2320, 2368, 2383, 2403, 2636
BstMBI GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
BstMCI CGRYCG 1 cut(s) 2636
BstMWI GCNNNNNNNGC 4 cut(s) 182, 1171, 1214, 1225
BstNI CCWGG 3 cut(s) 1389, 1632, 2081
BstNSI RCATGY 2 cut(s) 808, 1133
BstSCI CCNGG 4 cut(s) 1016, 1387, 1630, 2079
BstSFI CTRYAG 3 cut(s) 1878, 1923, 2468
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 2 cut(s) 584, 918
BstV1I GCAGC 4 cut(s) 53, 497, 1833, 2562
BstV2I GAAGAC 1 cut(s) 1511
BstX2I RGATCY 1 cut(s) 1383
BstXI CCANNNNNNTGG 1 cut(s) 2667
BstYI RGATCY 1 cut(s) 1383
BsuI GTATCC 1 cut(s) 2203
BsuRI GGCC 7 cut(s) 141, 200, 395, 667, 915, 929, 1601
BtgI CCRYGG 2 cut(s) 489, 916
BtgZI GCGATG 2 cut(s) 599, 1373
BtrI CACGTC 1 cut(s) 1073
BtsCI GGATG 7 cut(s) 979, 1380, 1806, 1816, 2308, 2497, 2650
BtsI GCAGTG 1 cut(s) 2572
BtsIMutI CAGTG 3 cut(s) 1210, 1762, 2572
Cac8I GCNNGC 3 cut(s) 32, 403, 1210
CciI TCATGA 1 cut(s) 1909
CfoI GCGC 4 cut(s) 584, 721, 796, 1219
Cfr10I RCCGGY 2 cut(s) 299, 401
Cfr13I GGNCC 2 cut(s) 139, 1735
Cfr42I CCGCGG 1 cut(s) 919
CseI GACGC 1 cut(s) 394
Csp6I GTAC 8 cut(s) 502, 705, 1042, 1076, 1696, 1747, 1814, 2225
CspAI ACCGGT 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 2310, 2345
CviQI GTAC 8 cut(s) 502, 705, 1042, 1076, 1696, 1747, 1814, 2225
DdeI CTNAG 6 cut(s) 157, 294, 1080, 1692, 1704, 2228
DpnI GATC 9 cut(s) 1085, 1202, 1385, 1983, 2319, 2367, 2382, 2402, 2635
DpnII GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
DraI TTTAAA 2 cut(s) 1578, 2098
EaeI YGGCCR 2 cut(s) 913, 1599
Eam1104I CTCTTC 3 cut(s) 220, 272, 918
EarI CTCTTC 3 cut(s) 220, 272, 918
Ecl136II GAGCTC 1 cut(s) 1228
Eco130I CCWWGG 4 cut(s) 489, 1251, 1796, 2526
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 1230
Eco47I GGWCC 1 cut(s) 1735
Eco53kI GAGCTC 1 cut(s) 1228
Eco57I CTGAAG 3 cut(s) 1030, 2204, 2286
EcoICRI GAGCTC 1 cut(s) 1228
EcoRII CCWGG 3 cut(s) 1387, 1630, 2079
EcoT14I CCWWGG 4 cut(s) 489, 1251, 1796, 2526
EcoT22I ATGCAT 3 cut(s) 912, 1281, 2258
EcoT38I GRGCYC 1 cut(s) 1230
ErhI CCWWGG 4 cut(s) 489, 1251, 1796, 2526
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FaqI GGGAC 4 cut(s) 425, 578, 1748, 1880
FauI CCCGC 3 cut(s) 649, 854, 1656
FauNDI CATATG 1 cut(s) 498
FbaI TGATCA 2 cut(s) 1200, 2400
Fnu4HI GCNGC 5 cut(s) 67, 511, 916, 1847, 2551
FokI GGATG 7 cut(s) 986, 1387, 1813, 1823, 2315, 2504, 2637
FriOI GRGCYC 1 cut(s) 1230
Fsp4HI GCNGC 5 cut(s) 67, 511, 916, 1847, 2551
FspAI RTGCGCAY 1 cut(s) 1218
FspBI CTAG 3 cut(s) 618, 1350, 1544
FspI TGCGCA 1 cut(s) 1218
GlaI GCGC 4 cut(s) 583, 720, 795, 1218
GluI GCNGC 5 cut(s) 67, 511, 916, 1847, 2551
GsuI CTGGAG 1 cut(s) 2106
HaeIII GGCC 7 cut(s) 141, 200, 395, 667, 915, 929, 1601
HapII CCGG 6 cut(s) 233, 300, 402, 668, 971, 1017
HgaI GACGC 1 cut(s) 394
HhaI GCGC 4 cut(s) 584, 721, 796, 1219
Hin1I GRCGYC 2 cut(s) 93, 405
Hin6I GCGC 4 cut(s) 582, 719, 794, 1217
HinP1I GCGC 4 cut(s) 582, 719, 794, 1217
HindIII AAGCTT 2 cut(s) 318, 1232
HpaII CCGG 6 cut(s) 233, 300, 402, 668, 971, 1017
HphI GGTGA 8 cut(s) 157, 362, 364, 460, 476, 1235, 1240, 1741
Hpy166II GTNNAC 4 cut(s) 533, 705, 1076, 2314
Hpy188III TCNNGA 5 cut(s) 1618, 1718, 1910, 1933, 2384
Hpy8I GTNNAC 4 cut(s) 533, 705, 1076, 2314
Hpy99I CGWCG 3 cut(s) 95, 98, 410
HpyAV CCTTC 3 cut(s) 229, 532, 2631
HpyCH4III ACNGT 8 cut(s) 87, 277, 506, 1458, 1757, 1882, 1902, 2472
HpyCH4IV ACGT 3 cut(s) 93, 1072, 2610
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 1171, 1214, 1225
HpyF3I CTNAG 6 cut(s) 157, 294, 1080, 1692, 1704, 2228
HpySE526I ACGT 3 cut(s) 93, 1072, 2610
Hsp92I GRCGYC 2 cut(s) 93, 405
HspAI GCGC 4 cut(s) 582, 719, 794, 1217
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Ksp22I TGATCA 2 cut(s) 1200, 2400
KspI CCGCGG 1 cut(s) 919
Kzo9I GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
LmnI GCTCC 5 cut(s) 579, 781, 1392, 2011, 2218
Lsp1109I GCAGC 4 cut(s) 53, 497, 1833, 2562
LweI GCATC 8 cut(s) 247, 613, 636, 1254, 1561, 1791, 1821, 2304
MaeI CTAG 3 cut(s) 618, 1350, 1544
MaeII ACGT 3 cut(s) 93, 1072, 2610
MaeIII GTNAC 1 cut(s) 2140
MalI GATC 9 cut(s) 1085, 1202, 1385, 1983, 2319, 2367, 2382, 2402, 2635
MbiI CCGCTC 1 cut(s) 861
MboI GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
MfeI CAATTG 2 cut(s) 870, 2554
MflI RGATCY 1 cut(s) 1383
MhlI GDGCHC 4 cut(s) 165, 535, 1230, 1397
MlsI TGGCCA 1 cut(s) 1601
MluNI TGGCCA 1 cut(s) 1601
MlyI GAGTC 7 cut(s) 560, 752, 966, 1292, 1897, 2097, 2632
MmeI TCCRAC 3 cut(s) 106, 213, 1329
Mox20I TGGCCA 1 cut(s) 1601
Mph1103I ATGCAT 3 cut(s) 912, 1281, 2258
MroNI GCCGGC 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 542
MscI TGGCCA 1 cut(s) 1601
MseI TTAA 7 cut(s) 42, 345, 1259, 1577, 2097, 2237, 2343
MslI CAYNNNNRTG 2 cut(s) 803, 2446
Msp20I TGGCCA 1 cut(s) 1601
MspA1I CMGCKG 2 cut(s) 918, 1846
MspI CCGG 6 cut(s) 233, 300, 402, 668, 971, 1017
MspR9I CCNGG 4 cut(s) 1018, 1389, 1632, 2081
MunI CAATTG 2 cut(s) 870, 2554
MvaI CCWGG 3 cut(s) 1389, 1632, 2081
MvnI CGCG 2 cut(s) 584, 918
MwoI GCNNNNNNNGC 4 cut(s) 182, 1171, 1214, 1225
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 1 cut(s) 1018
NcoI CCATGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 498
NdeII GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
NgoMIV GCCGGC 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 969, 1736
NmeAIII GCCGAG 1 cut(s) 1145
NsbI TGCGCA 1 cut(s) 1218
NsiI ATGCAT 3 cut(s) 912, 1281, 2258
NspI RCATGY 2 cut(s) 808, 1133
NspV TTCGAA 1 cut(s) 2475
PagI TCATGA 1 cut(s) 1909
PasI CCCWGGG 1 cut(s) 1388
PceI AGGCCT 1 cut(s) 395
PciI ACATGT 2 cut(s) 804, 1129
PcsI WCGNNNNNNNCGW 2 cut(s) 581, 2376
PdiI GCCGGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 7 cut(s) 236, 766, 1657, 1949, 2166, 2179, 2188
PflMI CCANNNNNTGG 1 cut(s) 2151
PinAI ACCGGT 1 cut(s) 299
PkrI GCNGC 5 cut(s) 68, 512, 917, 1848, 2552
Ple19I CGATCG 1 cut(s) 2636
PleI GAGTC 7 cut(s) 560, 751, 965, 1291, 1896, 2096, 2632
PpsI GAGTC 7 cut(s) 560, 751, 965, 1291, 1896, 2096, 2632
Ppu21I YACGTR 1 cut(s) 2611
PscI ACATGT 2 cut(s) 804, 1129
PsiI TTATAA 1 cut(s) 1713
Psp124BI GAGCTC 1 cut(s) 1230
Psp6I CCWGG 3 cut(s) 1387, 1630, 2079
PspGI CCWGG 3 cut(s) 1387, 1630, 2079
PspN4I GGNNCC 2 cut(s) 969, 1736
PspPI GGNCC 2 cut(s) 139, 1735
PsuI RGATCY 1 cut(s) 1383
PvuI CGATCG 1 cut(s) 2636
PvuII CAGCTG 1 cut(s) 1846
RsaI GTAC 8 cut(s) 503, 706, 1043, 1077, 1697, 1748, 1815, 2226
RsaNI GTAC 8 cut(s) 502, 705, 1042, 1076, 1696, 1747, 1814, 2225
RseI CAYNNNNRTG 2 cut(s) 803, 2446
SacI GAGCTC 1 cut(s) 1230
SacII CCGCGG 1 cut(s) 919
SaqAI TTAA 7 cut(s) 42, 345, 1259, 1577, 2097, 2237, 2343
SatI GCNGC 5 cut(s) 67, 511, 916, 1847, 2551
Sau3AI GATC 9 cut(s) 1083, 1200, 1383, 1981, 2317, 2365, 2380, 2400, 2633
Sau96I GGNCC 2 cut(s) 139, 1735
SchI GAGTC 7 cut(s) 560, 752, 966, 1292, 1897, 2097, 2632
ScrFI CCNGG 4 cut(s) 1018, 1389, 1632, 2081
SduI GDGCHC 4 cut(s) 165, 535, 1230, 1397
SfaNI GCATC 8 cut(s) 247, 613, 636, 1254, 1561, 1791, 1821, 2304
SfcI CTRYAG 3 cut(s) 1878, 1923, 2468
Sfr303I CCGCGG 1 cut(s) 919
SfuI TTCGAA 1 cut(s) 2475
SgrBI CCGCGG 1 cut(s) 919
SinI GGWCC 1 cut(s) 1735
SmiMI CAYNNNNRTG 2 cut(s) 803, 2446
SmlI CTYRAG 3 cut(s) 389, 722, 1229
SmoI CTYRAG 3 cut(s) 389, 722, 1229
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 8 cut(s) 183, 513, 626, 656, 861, 916, 918, 1663
SspI AATATT 1 cut(s) 8
SspMI CTAG 3 cut(s) 618, 1350, 1544
SstI GAGCTC 1 cut(s) 1230
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 4 cut(s) 1016, 1387, 1630, 2079
StyI CCWWGG 4 cut(s) 489, 1251, 1796, 2526
TaaI ACNGT 8 cut(s) 87, 277, 506, 1458, 1757, 1882, 1902, 2472
TaiI ACGT 3 cut(s) 96, 1075, 2613
TaqII GACCGA 1 cut(s) 1752
TatI WGTACW 4 cut(s) 501, 1695, 1813, 2224
TauI GCSGC 1 cut(s) 918
TfiI GAWTC 7 cut(s) 236, 766, 1657, 1949, 2166, 2179, 2188
Tru1I TTAA 7 cut(s) 42, 345, 1259, 1577, 2097, 2237, 2343
Tru9I TTAA 7 cut(s) 42, 345, 1259, 1577, 2097, 2237, 2343
TscAI CASTG 3 cut(s) 1210, 1762, 2579
TseI GCWGC 4 cut(s) 66, 510, 1846, 2550
TspGWI ACGGA 2 cut(s) 550, 2231
TspRI CASTG 3 cut(s) 1210, 1762, 2579
Van91I CCANNNNNTGG 1 cut(s) 2151
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 1735
XapI RAATTY 7 cut(s) 368, 418, 452, 1064, 1419, 1427, 2498
XceI RCATGY 2 cut(s) 808, 1133
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 3 cut(s) 618, 1350, 1544
ZraI GACGTC 1 cut(s) 94
Zsp2I ATGCAT 3 cut(s) 912, 1281, 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.