Rmu_sc0008092.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008092.1
OG0008899
Physical Location & Seq
Reverse (-)
40733 .. 42048
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008092.1_g000009.1.cds

Sequence Viewer

Length: 510 bp
atggtttgcgtggattggtttgatgtgttggcaattgatggggatgtaaggagaggtcagttcctccttgagccaccggtggcccaaaacacttactccaagtgtcgcgggagtagggttccttggggaggcggcgctgtggtaatccggtctccgggtcgtcacatgcctgattcacctataaggcaagctgaggtggtaaagctagtgacaacaccatcaagaagagctccaactgttgaccttgctgataagagtaagaagaaagtagagttagagcaactagttgatgaagatgagagtgaagatgatgggtggtttcaggaagagcctgaagaggagaaagcagatgatggtaaagaaggtggttgggaaacttctgatgatgataattacaaccctgcaaaagatgatgaagagtatgcagggtatggggtggatgatgattttctctttgactggctaacagaagctaaagttgtgtctaatcaaggggagggcccttattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.94

Weight (kDa)

4.24

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 108
AciI CCGC 2 cut(s) 108, 132
AcuI CTGAAG 1 cut(s) 354
AfiI CCNNNNNNNGG 2 cut(s) 128, 154
AgeI ACCGGT 1 cut(s) 76
AhlI ACTAGT 1 cut(s) 283
AluBI AGCT 4 cut(s) 191, 205, 230, 473
AluI AGCT 4 cut(s) 191, 205, 230, 473
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 1 cut(s) 156
AoxI GGCC 2 cut(s) 81, 499
ApaI GGGCCC 1 cut(s) 503
AsiGI ACCGGT 1 cut(s) 76
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 82, 499, 500
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 503
BanII GRGCYC 2 cut(s) 232, 503
Bbv12I GWGCWC 1 cut(s) 232
BbvCI CCTCAGC 1 cut(s) 192
BccI CCATC 4 cut(s) 32, 226, 305, 347
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 156
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 2 cut(s) 206, 284
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 82, 499, 500
BmiI GGNNCC 2 cut(s) 120, 501
BmrFI CCNGG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 122
BsaWI WCCGGW 2 cut(s) 76, 147
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 154
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 2 cut(s) 49, 445
BseLI CCNNNNNNNGG 2 cut(s) 128, 154
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 83, 501
BshTI ACCGGT 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 3 cut(s) 77, 148, 155
BslI CCNNNNNNNGG 2 cut(s) 128, 154
BsmAI GTCTC 1 cut(s) 156
BsnI GGCC 2 cut(s) 83, 501
Bso31I GGTCTC 1 cut(s) 156
Bsp120I GGGCCC 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 232, 503
BspACI CCGC 2 cut(s) 108, 132
BspANI GGCC 2 cut(s) 83, 501
BspCNI CTCAG 1 cut(s) 184
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 120, 501
BspQI GCTCTTC 2 cut(s) 220, 321
BspTNI GGTCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 4 cut(s) 220, 321, 330, 411
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 192
BstENI CCTNNNNNAGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 49, 445
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 503
BstUI CGCG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 83, 501
BtsCI GGATG 2 cut(s) 49, 445
Cac8I GCNNGC 1 cut(s) 189
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 3 cut(s) 82, 499, 500
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 1 cut(s) 166
CviJI RGCY 9 cut(s) 73, 83, 191, 205, 230, 331, 463, 473, 501
CviKI_1 RGCY 9 cut(s) 73, 83, 191, 205, 230, 331, 463, 473, 501
DdeI CTNAG 1 cut(s) 192
Eam1104I CTCTTC 4 cut(s) 220, 321, 330, 411
EarI CTCTTC 4 cut(s) 220, 321, 330, 411
Ecl136II GAGCTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 122
Eco24I GRGCYC 2 cut(s) 232, 503
Eco31I GGTCTC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoO109I RGGNCCY 2 cut(s) 499, 500
EcoT14I CCWWGG 1 cut(s) 122
EcoT38I GRGCYC 2 cut(s) 232, 503
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 1 cut(s) 169
FaiI YATR 4 cut(s) 167, 182, 423, 432
FatI CATG 1 cut(s) 165
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 2 cut(s) 56, 452
FriOI GRGCYC 2 cut(s) 232, 503
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 2 cut(s) 206, 284
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 2 cut(s) 83, 501
HapII CCGG 3 cut(s) 77, 148, 155
HhaI GCGC 1 cut(s) 137
Hin1II CATG 1 cut(s) 169
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 173
HpaII CCGG 3 cut(s) 77, 148, 155
HphI GGTGA 1 cut(s) 168
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 382
Hpy188III TCNNGA 2 cut(s) 222, 323
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 356
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 2 cut(s) 404, 425
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 1 cut(s) 169
HspAI GCGC 1 cut(s) 135
LguI GCTCTTC 2 cut(s) 220, 321
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 9 cut(s) 90, 161, 168, 183, 308, 345, 411, 414, 445
MaeI CTAG 2 cut(s) 206, 284
MaeIII GTNAC 2 cut(s) 161, 208
MboII GAAGA 7 cut(s) 237, 274, 305, 317, 338, 347, 428
MfeI CAATTG 1 cut(s) 33
MhlI GDGCHC 2 cut(s) 232, 503
MluCI AATT 2 cut(s) 33, 391
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 6 cut(s) 47, 74, 122, 187, 331, 490
MspI CCGG 3 cut(s) 77, 148, 155
MspR9I CCNGG 1 cut(s) 156
MunI CAATTG 1 cut(s) 33
MvnI CGCG 1 cut(s) 108
NciI CCSGG 1 cut(s) 156
NlaIII CATG 1 cut(s) 169
NlaIV GGNNCC 2 cut(s) 120, 501
NmuCI GTSAC 2 cut(s) 161, 208
NspI RCATGY 1 cut(s) 169
PciSI GCTCTTC 2 cut(s) 220, 321
PfeI GAWTC 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 120, 501
PspOMI GGGCCC 1 cut(s) 499
PspPI GGNCC 3 cut(s) 82, 499, 500
SacI GAGCTC 1 cut(s) 232
SapI GCTCTTC 2 cut(s) 220, 321
SatI GCNGC 1 cut(s) 133
Sau96I GGNCC 3 cut(s) 82, 499, 500
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 2 cut(s) 232, 503
SetI ASST 9 cut(s) 58, 181, 193, 198, 207, 232, 246, 367, 475
SgrAI CRCCGGYG 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 283
Sse9I AATT 2 cut(s) 33, 391
SsiI CCGC 2 cut(s) 108, 132
SspMI CTAG 2 cut(s) 206, 284
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 238
TasI AATT 2 cut(s) 33, 391
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 173
TseFI GTSAC 2 cut(s) 161, 208
Tsp45I GTSAC 2 cut(s) 161, 208
TspDTI ATGAA 2 cut(s) 306, 429
XagI CCTNNNNNAGG 1 cut(s) 126
XceI RCATGY 1 cut(s) 169
XspI CTAG 2 cut(s) 206, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.