Rmu_sc0014416.1_g000002

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014416.1
OG0015755
Physical Location & Seq
Reverse (-)
11998 .. 13137
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014416.1_g000002.1.cds

Sequence Viewer

Length: 534 bp
atggagcggcagaagcggttggtggtgtgctccgagaatgaagcgctggcagagtacatgttgagtaagtggcaggaaatggcagacaagaagtccaaaggaatctctgaaaacattgaaaagactcttaccaaggcatattccaatgtctgcaactctaagaatcctgttaaaaccctcaaagagttttcccaaatcaagggtgttgggaaatggattttgaagcttatgcaagggtattttgacaccggttcaggcagttctgaaccagaagatgtaactaaaaagggtaagagaaccaaaggaagcaaacactatgttccacaaaagaattctgtagcatatgctttactgatcacactttacaggctcgagaatcatattcaggattccataggccttgacatgcttactgacatcacgaatccacctgtgatatctagagccagttgttcccaatggagtaaaatccaatttgttcaagttactcatcctgaaagagaccaagaaattagggttagtttcggagcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

177

Amino Acids

20.06

Weight (kDa)

9.27

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 7
AciI CCGC 2 cut(s) 7, 16
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 1 cut(s) 56
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 57
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 3 cut(s) 119, 223, 482
AhdI GACNNNNNGTC 1 cut(s) 91
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 495
Ama87I CYCGRG 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 1 cut(s) 397
ApoI RAATTY 1 cut(s) 331
AsiGI ACCGGT 1 cut(s) 248
AspLEI GCGC 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 32
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 47
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 371
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 447
BshFI GGCC 1 cut(s) 399
BshTI ACCGGT 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 495
BsnI GGCC 1 cut(s) 399
Bso31I GGTCTC 1 cut(s) 495
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 354
BspACI CCGC 2 cut(s) 7, 16
BspANI GGCC 1 cut(s) 399
BspTNI GGTCTC 1 cut(s) 495
BsrBI CCGCTC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 447
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 490
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 1 cut(s) 354
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 61, 409
BstSFI CTRYAG 1 cut(s) 336
BsuRI GGCC 1 cut(s) 399
BtsCI GGATG 1 cut(s) 490
Cac8I GCNNGC 1 cut(s) 48
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 248
Csp6I GTAC 1 cut(s) 55
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 2 cut(s) 58, 406
CviJI RGCY 4 cut(s) 226, 370, 399, 446
CviKI_1 RGCY 4 cut(s) 226, 370, 399, 446
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 159
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
DriI GACNNNNNGTC 1 cut(s) 91
Eam1105I GACNNNNNGTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 495
Eco32I GATATC 1 cut(s) 438
Eco47III AGCGCT 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 371
EcoRI GAATTC 1 cut(s) 331
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 2 cut(s) 61, 409
FaiI YATR 9 cut(s) 59, 139, 230, 318, 343, 345, 381, 395, 407
FatI CATG 2 cut(s) 57, 405
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 1 cut(s) 399
HapII CCGG 1 cut(s) 249
HhaI GCGC 1 cut(s) 46
Hin1II CATG 2 cut(s) 61, 409
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 6 cut(s) 102, 124, 163, 376, 389, 424
HpaII CCGG 1 cut(s) 249
Hpy188I TCNGA 4 cut(s) 34, 109, 265, 527
Hpy188III TCNNGA 5 cut(s) 373, 386, 421, 441, 494
HpyCH4V TGCA 2 cut(s) 153, 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 159
Hsp92II CATG 2 cut(s) 61, 409
HspAI GCGC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 1 cut(s) 354
LmnI GCTCC 3 cut(s) 4, 35, 527
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 277, 484
MalI GATC 1 cut(s) 356
MbiI CCGCTC 1 cut(s) 7
MboI GATC 1 cut(s) 354
MboII GAAGA 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 3 cut(s) 331, 473, 510
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 1 cut(s) 188
MseI TTAA 1 cut(s) 171
MspI CCGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeI CATATG 1 cut(s) 343
NdeII GATC 1 cut(s) 354
NlaIII CATG 2 cut(s) 61, 409
NspI RCATGY 2 cut(s) 61, 409
PaeR7I CTCGAG 1 cut(s) 371
PceI AGGCCT 1 cut(s) 399
PciI ACATGT 1 cut(s) 57
PfeI GAWTC 5 cut(s) 102, 163, 376, 389, 424
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PscI ACATGT 1 cut(s) 57
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 1 cut(s) 354
SchI GAGTC 1 cut(s) 118
SduI GDGCHC 1 cut(s) 32
SetI ASST 2 cut(s) 228, 433
SfcI CTRYAG 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 3 cut(s) 331, 473, 510
SseBI AGGCCT 1 cut(s) 399
SsiI CCGC 2 cut(s) 7, 16
SspMI CTAG 1 cut(s) 441
StuI AGGCCT 1 cut(s) 399
StyI CCWWGG 1 cut(s) 132
TaqI TCGA 1 cut(s) 372
TasI AATT 3 cut(s) 331, 473, 510
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 5 cut(s) 102, 163, 376, 389, 424
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TspDTI ATGAA 1 cut(s) 54
XapI RAATTY 1 cut(s) 331
XbaI TCTAGA 1 cut(s) 440
XceI RCATGY 2 cut(s) 61, 409
XhoI CTCGAG 1 cut(s) 371
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.