Rh3AG216000

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015755
Physical Location & Seq
Reverse (-)
21826757 .. 21829682
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG216000.1

Sequence Viewer

Length: 789 bp
ATGATGGAGACCTCCTACACCGTTAACAACAAGCCTCCGATCAAAGAGATCGCTCTCGCCATTGCCCTTCTCGTCCTTGGCATTGTCGTCGGCTGCCTCATGGCCTATAACCGCGAATGTTCTTTGCCATTCTTGGTGGGATATTGTTCATTCAAGGCTCCTATTACGCGCGGATTACTTACTATGCTTACAAATGATACAAGGGATTCTCTTTCACCAACATACTGCGTATGTAGTAGCAGCTCCTTTACTCTGCTTCAACTTCAAATGGGGACCTATTTAGTTCACACATTGACTGTCAAAACTGAGAGACAAGCCTGCCCTTATCACGATTCAATGGAGCGGCAGAAGCGGTTGGTGGTGTGCTCCGAGAATGAAGCGCTGGCAGAGTACATGTTGAGTAAGTGGCAGGAAATGGCAGACAAGAAGGCCAAAGGAATCTCTGAAAACATTGAAAAGACTCTTACCAAGGCATACTCCAATGTCTGCAACTCTAAGAATCCTGTTAAAACCCTTAAAGAGTTTTCCCAAATCAGGGGTGTTGGGAAATGGATTTTGAAGCTTATGCAAGGGTATTTTGACACCGGTTCAGGCAGTTCTGAACCAGAAGATGTAACTAAAAAGGGTAAGAGAACCAAAGGAAGCAAACGCTATGTTCCACAAAAGAATTCTGTAGCATATGATTTACTGATCACACTTTACAGGTCTTTTGCAATCCAGAGGCTCAAATGGGTTTCCATCCTGATGCAGGAGCTTCATATTATCTTTCTCGTCTACCTGGCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

262

Amino Acids

29.62

Weight (kDa)

9.28

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 774
AccII CGCG 3 cut(s) 114, 169, 171
AciI CCGC 4 cut(s) 112, 171, 343, 352
AcsI RAATTY 1 cut(s) 667
AfaI GTAC 1 cut(s) 392
AfeI AGCGCT 1 cut(s) 381
AfiI CCNNNNNNNGG 3 cut(s) 534, 535, 748
AflIII ACRYGT 1 cut(s) 393
AgeI ACCGGT 1 cut(s) 584
AgsI TTSAA 6 cut(s) 154, 260, 266, 336, 455, 559
AjnI CCWGG 1 cut(s) 777
AluBI AGCT 3 cut(s) 243, 562, 754
AluI AGCT 3 cut(s) 243, 562, 754
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 2, 304
Aor51HI AGCGCT 1 cut(s) 381
AoxI GGCC 2 cut(s) 102, 429
ApeKI GCWGC 2 cut(s) 93, 240
ApoI RAATTY 1 cut(s) 667
AsiGI ACCGGT 1 cut(s) 584
AspLEI GCGC 2 cut(s) 171, 382
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 207
AvaII GGWCC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 80, 252
BccI CCATC 1 cut(s) 746
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 779
BclI TGATCA 1 cut(s) 690
BcoDI GTCTC 2 cut(s) 2, 304
BfmI CTRYAG 1 cut(s) 672
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 3 cut(s) 94, 241, 344
BlsI GCNGC 3 cut(s) 95, 242, 345
Bme1390I CCNGG 1 cut(s) 779
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 159, 274
BmrFI CCNGG 1 cut(s) 779
BmsI GCATC 1 cut(s) 735
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 2 cut(s) 76, 468
BsaWI WCCGGW 1 cut(s) 584
Bsc4I CCNNNNNNNGG 3 cut(s) 534, 535, 748
Bse118I RCCGGY 1 cut(s) 584
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 779
BseDI CCNNGG 2 cut(s) 76, 468
BseGI GGATG 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 534, 535, 748
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 80, 252
Bsh1236I CGCG 3 cut(s) 114, 169, 171
BshFI GGCC 2 cut(s) 104, 431
BshTI ACCGGT 1 cut(s) 584
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 3 cut(s) 534, 535, 748
BsmAI GTCTC 2 cut(s) 2, 304
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 2 cut(s) 104, 431
Bso31I GGTCTC 1 cut(s) 2
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 3 cut(s) 39, 48, 690
BspACI CCGC 4 cut(s) 112, 171, 343, 352
BspANI GGCC 2 cut(s) 104, 431
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 3 cut(s) 114, 169, 171
BspLI GGNNCC 2 cut(s) 159, 274
BspTNI GGTCTC 1 cut(s) 2
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 584
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 2 cut(s) 76, 468
BssMI GATC 3 cut(s) 39, 48, 690
BssT1I CCWWGG 2 cut(s) 76, 468
Bst2UI CCWGG 1 cut(s) 779
Bst4CI ACNGT 2 cut(s) 22, 298
BstC8I GCNNGC 2 cut(s) 319, 384
BstDEI CTNAG 3 cut(s) 306, 495, 786
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 1 cut(s) 738
BstFNI CGCG 3 cut(s) 114, 169, 171
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 2 cut(s) 171, 382
BstKTI GATC 3 cut(s) 42, 51, 693
BstMAI GTCTC 2 cut(s) 2, 304
BstMBI GATC 3 cut(s) 39, 48, 690
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 779
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 777
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 3 cut(s) 114, 169, 171
BstV1I GCAGC 2 cut(s) 80, 252
BsuRI GGCC 2 cut(s) 104, 431
BtsCI GGATG 1 cut(s) 738
Cac8I GCNNGC 2 cut(s) 319, 384
CfoI GCGC 2 cut(s) 171, 382
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 584
CviAII CATG 2 cut(s) 100, 394
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 306, 495, 786
DpnI GATC 3 cut(s) 41, 50, 692
DpnII GATC 3 cut(s) 39, 48, 690
Eco130I CCWWGG 2 cut(s) 76, 468
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 1 cut(s) 273
Eco47III AGCGCT 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 777
EcoT14I CCWWGG 2 cut(s) 76, 468
ErhI CCWWGG 2 cut(s) 76, 468
FaeI CATG 2 cut(s) 103, 397
FaqI GGGAC 1 cut(s) 286
FatI CATG 2 cut(s) 99, 393
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 690
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 3 cut(s) 94, 241, 344
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 3 cut(s) 94, 241, 344
GlaI GCGC 2 cut(s) 170, 381
GluI GCNGC 3 cut(s) 94, 241, 344
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 2 cut(s) 104, 431
HapII CCGG 1 cut(s) 585
HhaI GCGC 2 cut(s) 171, 382
Hin1II CATG 2 cut(s) 103, 397
Hin6I GCGC 2 cut(s) 169, 380
HinP1I GCGC 2 cut(s) 169, 380
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 5 cut(s) 206, 332, 438, 460, 499
HpaI GTTAAC 1 cut(s) 25
HpaII CCGG 1 cut(s) 585
HphI GGTGA 1 cut(s) 207
Hpy166II GTNNAC 3 cut(s) 25, 286, 775
Hpy188I TCNGA 4 cut(s) 39, 370, 445, 601
Hpy188III TCNNGA 3 cut(s) 329, 718, 742
Hpy8I GTNNAC 3 cut(s) 25, 286, 775
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 77, 421
HpyCH4III ACNGT 2 cut(s) 22, 298
HpyCH4V TGCA 4 cut(s) 489, 568, 713, 748
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 3 cut(s) 306, 495, 786
Hsp92II CATG 2 cut(s) 103, 397
HspAI GCGC 2 cut(s) 169, 380
Ksp22I TGATCA 1 cut(s) 690
KspAI GTTAAC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 39, 48, 690
LmnI GCTCC 5 cut(s) 163, 248, 340, 371, 751
Lsp1109I GCAGC 2 cut(s) 80, 252
LweI GCATC 1 cut(s) 735
MaeIII GTNAC 1 cut(s) 613
MalI GATC 3 cut(s) 41, 50, 692
MbiI CCGCTC 1 cut(s) 343
MboI GATC 3 cut(s) 39, 48, 690
MboII GAAGA 1 cut(s) 620
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 1 cut(s) 667
MlyI GAGTC 1 cut(s) 454
MnlI CCTC 4 cut(s) 22, 45, 107, 714
MseI TTAA 3 cut(s) 24, 507, 516
MslI CAYNNNNRTG 1 cut(s) 743
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 1 cut(s) 779
MvaI CCWGG 1 cut(s) 779
MvnI CGCG 3 cut(s) 114, 169, 171
MwoI GCNNNNNNNGC 1 cut(s) 349
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 39, 48, 690
NlaIII CATG 2 cut(s) 103, 397
NlaIV GGNNCC 2 cut(s) 159, 274
NspI RCATGY 1 cut(s) 397
PciI ACATGT 1 cut(s) 393
PfeI GAWTC 4 cut(s) 206, 332, 438, 499
PinAI ACCGGT 1 cut(s) 584
PkrI GCNGC 3 cut(s) 95, 242, 345
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 273
PscI ACATGT 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 777
PspGI CCWGG 1 cut(s) 777
PspN4I GGNNCC 2 cut(s) 159, 274
PspPI GGNCC 1 cut(s) 273
PspPPI RGGWCCY 1 cut(s) 273
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 743
SaqAI TTAA 3 cut(s) 24, 507, 516
SatI GCNGC 3 cut(s) 94, 241, 344
Sau3AI GATC 3 cut(s) 39, 48, 690
Sau96I GGNCC 1 cut(s) 273
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 1 cut(s) 779
SduI GDGCHC 1 cut(s) 368
SetI ASST 7 cut(s) 14, 245, 278, 564, 707, 756, 780
SfaNI GCATC 1 cut(s) 735
SfcI CTRYAG 1 cut(s) 672
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 743
Sse9I AATT 1 cut(s) 667
SsiI CCGC 4 cut(s) 112, 171, 343, 352
StyD4I CCNGG 1 cut(s) 777
StyI CCWWGG 2 cut(s) 76, 468
TaaI ACNGT 2 cut(s) 22, 298
TasI AATT 1 cut(s) 667
TatI WGTACW 1 cut(s) 390
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 4 cut(s) 206, 332, 438, 499
Tru1I TTAA 3 cut(s) 24, 507, 516
Tru9I TTAA 3 cut(s) 24, 507, 516
TseI GCWGC 2 cut(s) 93, 240
TspDTI ATGAA 3 cut(s) 138, 390, 746
VpaK11BI GGWCC 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 746
XapI RAATTY 1 cut(s) 667
XceI RCATGY 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.