Rmu_sc0016601.1_g000009

methylesterase 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016601.1
OG0007331
Physical Location & Seq
Reverse (-)
34579 .. 35558
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016601.1_g000009.1.cds

Sequence Viewer

Length: 561 bp
atgcaggtgatcttggtgggccatgattttggtggcgcgtgcatatcgtatgcaatggagttgtttccatctaaagttgcgaaagccatttttcttgctgctgcaatgttgaaaaatggacagagcacccttgatacgttctcccaacaggcaacttcagatgatcttatgcgacaggctcagatatttttgtatgcaaatgggaataatcagcctccaactgctattgatctagataaatcaatgttgaaggatttgttattcaaccaaagtccttccaaggatgttgcattggcatctgtttcaatgagaccgattccatttgccccagtcctggagaaagtttcactttctgattcgaaatatggatcagttaggcggttttatatagaaacattagaagacaatgccatacccgtcacagtccaagagagcatgataaatgcaagcccccctgaacaggttctgcggttaaaaggcgctgatcactcacctttcttctcaaagcctcaagcactgcacaagttattggtagagatctcaaagatttcttcaccgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

186

Amino Acids

20.35

Weight (kDa)

6.28

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 38
AciI CCGC 2 cut(s) 379, 469
AclWI GGATC 1 cut(s) 376
AcuI CTGAAG 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 334, 460
AgsI TTSAA 4 cut(s) 112, 250, 265, 306
AjnI CCWGG 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 376
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 1 cut(s) 19
ApeKI GCWGC 2 cut(s) 98, 101
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 2 cut(s) 38, 482
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 3 cut(s) 19, 483, 546
AsuII TTCGAA 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 2 cut(s) 85, 88
BccI CCATC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 335
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 483
BglII AGATCT 1 cut(s) 537
BisI GCNGC 2 cut(s) 99, 102
BlsI GCNGC 2 cut(s) 100, 103
Bme1390I CCNGG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 335
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 460
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 60, 111
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 334, 460
BseMI GCAATG 2 cut(s) 60, 111
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 85, 88
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 334, 460
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 1 cut(s) 21
Bso31I GGTCTC 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 6 cut(s) 9, 163, 229, 368, 484, 537
BspACI CCGC 2 cut(s) 379, 469
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 1 cut(s) 38
BspPI GGATC 1 cut(s) 376
BspT104I TTCGAA 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 60, 111
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 6 cut(s) 9, 163, 229, 368, 484, 537
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 424, 558
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 40, 448
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 2 cut(s) 38, 482
BstKTI GATC 6 cut(s) 12, 166, 232, 371, 487, 540
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 6 cut(s) 9, 163, 229, 368, 484, 537
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 333
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 85, 88
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 1 cut(s) 537
BsuRI GGCC 1 cut(s) 21
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 2 cut(s) 40, 448
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 2 cut(s) 38, 482
Cfr13I GGNCC 1 cut(s) 19
CviAII CATG 2 cut(s) 23, 436
CviJI RGCY 6 cut(s) 21, 86, 179, 214, 450, 508
CviKI_1 RGCY 6 cut(s) 21, 86, 179, 214, 450, 508
DdeI CTNAG 1 cut(s) 180
DpnI GATC 6 cut(s) 11, 165, 231, 370, 486, 539
DpnII GATC 6 cut(s) 9, 163, 229, 368, 484, 537
Eco130I CCWWGG 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 2 cut(s) 26, 439
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FatI CATG 2 cut(s) 22, 435
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 99, 102
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 99, 102
FspBI CTAG 1 cut(s) 233
GlaI GCGC 2 cut(s) 37, 481
GluI GCNGC 2 cut(s) 99, 102
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 1 cut(s) 21
HhaI GCGC 2 cut(s) 38, 482
Hin1II CATG 2 cut(s) 26, 439
Hin6I GCGC 2 cut(s) 36, 480
HinP1I GCGC 2 cut(s) 36, 480
HinfI GANTC 2 cut(s) 316, 356
HphI GGTGA 3 cut(s) 19, 483, 546
Hpy188I TCNGA 3 cut(s) 160, 183, 355
Hpy188III TCNNGA 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 244, 285
HpyCH4III ACNGT 2 cut(s) 424, 558
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 8 cut(s) 4, 42, 53, 104, 197, 290, 446, 520
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 2 cut(s) 26, 439
HspAI GCGC 2 cut(s) 36, 480
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 6 cut(s) 9, 163, 229, 368, 484, 537
LpnPI CCDG 7 cut(s) 134, 161, 320, 342, 347, 446, 468
Lsp1109I GCAGC 2 cut(s) 85, 88
LweI GCATC 1 cut(s) 305
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 418
MalI GATC 6 cut(s) 11, 165, 231, 370, 486, 539
MboI GATC 6 cut(s) 9, 163, 229, 368, 484, 537
MboII GAAGA 3 cut(s) 413, 490, 543
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 2 cut(s) 225, 519
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 38
NdeII GATC 6 cut(s) 9, 163, 229, 368, 484, 537
NlaIII CATG 2 cut(s) 26, 439
NmuCI GTSAC 1 cut(s) 418
NspV TTCGAA 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 2 cut(s) 316, 356
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 2 cut(s) 100, 103
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspPI GGNCC 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 537
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 2 cut(s) 99, 102
Sau3AI GATC 6 cut(s) 9, 163, 229, 368, 484, 537
Sau96I GGNCC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 1 cut(s) 128
SetI ASST 4 cut(s) 9, 140, 465, 496
SfaNI GCATC 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
SsiI CCGC 2 cut(s) 379, 469
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 424, 558
TaiI ACGT 1 cut(s) 140
TaqI TCGA 1 cut(s) 359
TaqII GACCGA 1 cut(s) 328
TfiI GAWTC 2 cut(s) 316, 356
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 98, 101
Tsp45I GTSAC 1 cut(s) 418
TspRI CASTG 1 cut(s) 522
XbaI TCTAGA 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.