Rmu_sc0018426.1_g000004

methylesterase 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018426.1
OG0007331
Physical Location & Seq
Forward (+)
11923 .. 13336
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018426.1_g000004.1.cds

Sequence Viewer

Length: 615 bp
atgggcaacctctgcgccatccttgcccccaaaccccccctcaagaaaaagcccattaagcgcctcccgaacccgccgcccgtacccaactcgagcacccgctggacccggatccgatcctcccgcaaacaaaacccggatgacgctctcctccaagaacaggctctcgccgccgcgatcctcttccagcagcaccagcaaaacggcggcggttctttgccgtttgatcgctccacatcgctgcgctaccccaataataacaactccagctccaagaagggcaacagcaacgtcttgccacgtagctccagcactcgggctcggtccttaaccgacccgctgctccagccgcaccagcttgtgaaccaggatgtaaagattgatgatcttgagaccaaccactttgttcttgtacatggaggcgggtttggtgcttggtgttggtataaaaccattgcacttctagaagagagtggttataaagttaatgccatagacttaactggttctggaattcattcatctgatacaaacaccattgcaagtctttcagaatatgtgaagcctcttactgattttcttgaaaagcttcctgatggaaaaaaggtaaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

204

Amino Acids

22.43

Weight (kDa)

9.6

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
AccII CGCG 1 cut(s) 176
AclWI GGATC 4 cut(s) 106, 111, 119, 172
AcsI RAATTY 1 cut(s) 513
AfaI GTAC 2 cut(s) 84, 414
AfiI CCNNNNNNNGG 2 cut(s) 160, 315
AgsI TTSAA 1 cut(s) 584
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 4 cut(s) 270, 306, 358, 589
AluI AGCT 4 cut(s) 270, 306, 358, 589
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 4 cut(s) 106, 111, 119, 172
Ama87I CYCGRG 2 cut(s) 91, 315
ApeKI GCWGC 3 cut(s) 190, 241, 340
ApoI RAATTY 1 cut(s) 513
Asp700I GAANNNNTTC 2 cut(s) 517, 588
AspLEI GCGC 3 cut(s) 17, 63, 246
AspS9I GGNCC 2 cut(s) 105, 324
AsuC2I CCSGG 2 cut(s) 109, 137
AvaI CYCGRG 2 cut(s) 91, 315
AvaII GGWCC 2 cut(s) 105, 324
BamHI GGATCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 202, 228, 327
BccI CCATC 2 cut(s) 26, 590
BceAI ACGGC 2 cut(s) 205, 220
BciT130I CCWGG 1 cut(s) 368
BcnI CCSGG 2 cut(s) 109, 137
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 464
BfoI RGCGCY 1 cut(s) 64
BisI GCNGC 8 cut(s) 77, 171, 174, 191, 208, 242, 341, 350
BlsI GCNGC 8 cut(s) 78, 172, 175, 192, 209, 243, 342, 351
Bme1390I CCNGG 3 cut(s) 109, 137, 368
Bme18I GGWCC 2 cut(s) 105, 324
BmeT110I CYCGRG 2 cut(s) 91, 315
BmgT120I GGNCC 2 cut(s) 105, 324
BmiI GGNNCC 2 cut(s) 107, 113
BmrFI CCNGG 3 cut(s) 109, 137, 368
BpmI CTGGAG 3 cut(s) 250, 292, 329
BpuEI CTTGAG 2 cut(s) 26, 410
BpuMI CCSGG 2 cut(s) 109, 137
BsaAI YACGTR 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 315
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 2 cut(s) 453, 537
BseBI CCWGG 1 cut(s) 368
BseGI GGATG 3 cut(s) 18, 145, 376
BseLI CCNNNNNNNGG 2 cut(s) 160, 315
BseMI GCAATG 2 cut(s) 453, 537
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 3 cut(s) 202, 228, 327
Bsh1236I CGCG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 91, 315
BsiSI CCGG 2 cut(s) 109, 137
BslI CCNNNNNNNGG 2 cut(s) 160, 315
BsmAI GTCTC 1 cut(s) 386
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 2 cut(s) 91, 315
Bsp1286I GDGCHC 2 cut(s) 98, 322
Bsp1407I TGTACA 1 cut(s) 412
Bsp143I GATC 5 cut(s) 111, 116, 177, 226, 385
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 107, 113
BspPI GGATC 4 cut(s) 106, 111, 119, 172
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 453, 537
BsrGI TGTACA 1 cut(s) 412
BsrI ACTGG 1 cut(s) 508
BssMI GATC 5 cut(s) 111, 116, 177, 226, 385
Bst2UI CCWGG 1 cut(s) 368
Bst6I CTCTTC 2 cut(s) 188, 462
BstAPI GCANNNNNTGC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 412
BstBAI YACGTR 1 cut(s) 302
BstF5I GGATG 3 cut(s) 18, 145, 376
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 3 cut(s) 17, 63, 246
BstKTI GATC 5 cut(s) 114, 119, 180, 229, 388
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 5 cut(s) 111, 116, 177, 226, 385
BstMWI GCNNNNNNNGC 8 cut(s) 12, 23, 58, 170, 196, 346, 349, 355
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 107, 135, 366
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 202, 228, 327
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 18, 145, 376
CfoI GCGC 3 cut(s) 17, 63, 246
Cfr13I GGNCC 2 cut(s) 105, 324
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 83, 413
CviAII CATG 1 cut(s) 416
CviJI RGCY 9 cut(s) 52, 164, 270, 306, 320, 349, 358, 565, 589
CviKI_1 RGCY 9 cut(s) 52, 164, 270, 306, 320, 349, 358, 565, 589
CviQI GTAC 2 cut(s) 83, 413
DpnI GATC 5 cut(s) 113, 118, 179, 228, 387
DpnII GATC 5 cut(s) 111, 116, 177, 226, 385
Eam1104I CTCTTC 2 cut(s) 188, 462
EarI CTCTTC 2 cut(s) 188, 462
Eco24I GRGCYC 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 105, 324
Eco88I CYCGRG 2 cut(s) 91, 315
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 322
FaeI CATG 1 cut(s) 419
FaiI YATR 5 cut(s) 417, 447, 480, 494, 558
FatI CATG 1 cut(s) 415
FauI CCCGC 5 cut(s) 81, 107, 131, 345, 416
Fnu4HI GCNGC 8 cut(s) 77, 171, 174, 191, 208, 242, 341, 350
FokI GGATG 3 cut(s) 5, 152, 383
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 8 cut(s) 77, 171, 174, 191, 208, 242, 341, 350
FspBI CTAG 1 cut(s) 464
GlaI GCGC 3 cut(s) 16, 62, 245
GluI GCNGC 8 cut(s) 77, 171, 174, 191, 208, 242, 341, 350
GsuI CTGGAG 3 cut(s) 250, 292, 329
HaeII RGCGCY 1 cut(s) 64
HapII CCGG 2 cut(s) 109, 137
HgaI GACGC 1 cut(s) 152
HhaI GCGC 3 cut(s) 17, 63, 246
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 3 cut(s) 15, 61, 244
HinP1I GCGC 3 cut(s) 15, 61, 244
HindIII AAGCTT 1 cut(s) 587
HpaII CCGG 2 cut(s) 109, 137
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 3 cut(s) 116, 526, 553
Hpy188III TCNNGA 7 cut(s) 43, 67, 389, 464, 510, 581, 593
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 271
HpyCH4IV ACGT 2 cut(s) 291, 301
HpyCH4V TGCA 2 cut(s) 458, 542
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 23, 58, 170, 196, 346, 349, 355
HpySE526I ACGT 2 cut(s) 291, 301
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 3 cut(s) 15, 61, 244
Kzo9I GATC 5 cut(s) 111, 116, 177, 226, 385
LmnI GCTCC 4 cut(s) 236, 275, 311, 348
Lsp1109I GCAGC 3 cut(s) 202, 228, 327
MaeI CTAG 1 cut(s) 464
MaeII ACGT 2 cut(s) 291, 301
MalI GATC 5 cut(s) 113, 118, 179, 228, 387
MboI GATC 5 cut(s) 111, 116, 177, 226, 385
MboII GAAGA 2 cut(s) 175, 479
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 2 cut(s) 98, 322
MluCI AATT 2 cut(s) 513, 610
MnlI CCTC 8 cut(s) 20, 50, 74, 130, 161, 191, 413, 576
MroXI GAANNNNTTC 2 cut(s) 517, 588
MseI TTAA 4 cut(s) 57, 329, 486, 500
MspA1I CMGCKG 2 cut(s) 102, 340
MspI CCGG 2 cut(s) 109, 137
MspR9I CCNGG 3 cut(s) 109, 137, 368
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 8 cut(s) 12, 23, 58, 170, 196, 346, 349, 355
NciI CCSGG 2 cut(s) 109, 137
NdeII GATC 5 cut(s) 111, 116, 177, 226, 385
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 2 cut(s) 107, 113
PaeR7I CTCGAG 1 cut(s) 91
PdmI GAANNNNTTC 2 cut(s) 517, 588
PkrI GCNGC 8 cut(s) 78, 172, 175, 192, 209, 243, 342, 351
Ppu21I YACGTR 1 cut(s) 302
PsiI TTATAA 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 2 cut(s) 107, 113
PspPI GGNCC 2 cut(s) 105, 324
PspXI VCTCGAGB 1 cut(s) 91
PsuI RGATCY 1 cut(s) 111
RsaI GTAC 2 cut(s) 84, 414
RsaNI GTAC 2 cut(s) 83, 413
SaqAI TTAA 4 cut(s) 57, 329, 486, 500
SatI GCNGC 8 cut(s) 77, 171, 174, 191, 208, 242, 341, 350
Sau3AI GATC 5 cut(s) 111, 116, 177, 226, 385
Sau96I GGNCC 2 cut(s) 105, 324
ScrFI CCNGG 3 cut(s) 109, 137, 368
SduI GDGCHC 2 cut(s) 98, 322
SetI ASST 8 cut(s) 12, 272, 294, 304, 308, 360, 591, 609
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 2 cut(s) 105, 324
SlaI CTCGAG 1 cut(s) 91
SmlI CTYRAG 3 cut(s) 41, 91, 389
SmoI CTYRAG 3 cut(s) 41, 91, 389
Sse9I AATT 2 cut(s) 513, 610
SspMI CTAG 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 107, 135, 366
TaiI ACGT 2 cut(s) 294, 304
TaqI TCGA 1 cut(s) 92
TaqII GACCGA 1 cut(s) 312
TasI AATT 2 cut(s) 513, 610
TatI WGTACW 1 cut(s) 412
TauI GCSGC 5 cut(s) 79, 173, 176, 210, 352
Tru1I TTAA 4 cut(s) 57, 329, 486, 500
Tru9I TTAA 4 cut(s) 57, 329, 486, 500
TseI GCWGC 3 cut(s) 190, 241, 340
TspDTI ATGAA 2 cut(s) 506, 510
VpaK11BI GGWCC 2 cut(s) 105, 324
XapI RAATTY 1 cut(s) 513
XbaI TCTAGA 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 91
XmnI GAANNNNTTC 2 cut(s) 517, 588
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.