Rmu_ssc0000024.1_g000045

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000024.1
OG0012383
Physical Location & Seq
Forward (+)
196823 .. 197875
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
atggccacactgactcacgccgccgccatcaccgcctcatcaccgctcgcaaaacccatctcttccagtcccaccaccaccaatcccctatcccatctctctttccctttctccccatccaaaaccctaaacctcaccgccaaaaccacctcaatcaaatcctcctcctcctccgatctctccaccaccaccgtaacgaccacctccatctcctcctccccgcaaaccctcaagtcccgcctccacaatggcgacaccctctacggcctcttcctcctcagcttctcccccaccctcgccgagatcgccggcctctccggctacgacttcgtcgtcgtcgacatggagcacggccccggcggaatctccgaagccctctcctgcctccgcgccctcgccgccacccaaaccccggcgatcctccggctgccggaatcctccccgacctgggccaagaaagccctcgatctcggcccgcaggggatcatgttcccgatgatcgacagctccaaggaggccgagaaagccgtctcctactgccgcttccctcccgccggcatccgcggctccgcacacaccgtcgtccgagcctccagctacggcatcgacgaggggtatttgagtaattacgaggaccaattgctgatcatgtgccaggtggagaccgaggagggcgtgaagagagcggaggagatcgcggccgtcgaaggcgttgactgcgtgcagatggggccgttggatctgagcgccagcctggggtacctgtgggacccggggaacaagaaggtccggaacgcgttgaaggttgccgagaaggccgtactgggcaccgacccccttgtgggtggggcctacttggctgggttcgctatgccccacgatgggccccaggacctgaggacacgtggctaccacatggtgtccggttccgtcgacgtcgggctgtttaggagcgcagcggtggaggacgtgaggaggtttcaggcgagcttgatcaacggctctgatgaggagcgtggggatgatgcagatgagaagtactggagcgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

350

Amino Acids

37.16

Weight (kDa)

5.16

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 88 - 318 9e-55 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AatII GACGTC 1 cut(s) 939
Acc65I GGTACC 1 cut(s) 759
AccB1I GGYRCC 2 cut(s) 759, 827
AccBSI CCGCTC 2 cut(s) 46, 686
AccI GTMKAC 2 cut(s) 339, 933
AccII CGCG 4 cut(s) 390, 564, 698, 797
AccIII TCCGGA 1 cut(s) 789
AclWI GGATC 3 cut(s) 412, 491, 747
AcoI YGGCCR 2 cut(s) 3, 699
AcvI CACGTG 1 cut(s) 905
AcyI GRCGYC 1 cut(s) 936
AdeI CACNNNGTG 1 cut(s) 919
AfaI GTAC 3 cut(s) 761, 822, 1040
AflIII ACRYGT 2 cut(s) 795, 902
AgsI TTSAA 1 cut(s) 802
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 4 cut(s) 446, 654, 753, 888
AluBI AGCT 4 cut(s) 282, 507, 597, 990
AluI AGCT 4 cut(s) 282, 507, 597, 990
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 535, 656
AlwI GGATC 3 cut(s) 412, 491, 747
AlwNI CAGNNNCTG 1 cut(s) 895
Ama87I CYCGRG 1 cut(s) 772
Aor13HI TCCGGA 1 cut(s) 789
ApaI GGGCCC 1 cut(s) 888
ApeKI GCWGC 2 cut(s) 427, 956
Asp718I GGTACC 1 cut(s) 759
AspLEI GCGC 3 cut(s) 392, 749, 956
AsuC2I CCSGG 4 cut(s) 357, 413, 773, 774
AsuHPI GGTGA 3 cut(s) 22, 33, 127
AvaI CYCGRG 1 cut(s) 772
AvaII GGWCC 4 cut(s) 634, 769, 787, 892
AxyI CCTNAGG 1 cut(s) 896
BaeGI GKGCMC 2 cut(s) 830, 888
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 759, 827
BanII GRGCYC 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 414, 968
BccI CCATC 7 cut(s) 35, 65, 102, 124, 215, 721, 875
BceAI ACGGC 8 cut(s) 280, 367, 512, 616, 686, 718, 803, 1015
BciT130I CCWGG 4 cut(s) 448, 656, 755, 890
BclI TGATCA 2 cut(s) 645, 993
BcnI CCSGG 4 cut(s) 357, 413, 773, 774
BcoDI GTCTC 2 cut(s) 535, 656
BfoI RGCGCY 1 cut(s) 750
BglI GCCNNNNNGGC 3 cut(s) 318, 815, 857
BisI GCNGC 8 cut(s) 21, 24, 399, 428, 541, 565, 699, 957
BlsI GCNGC 8 cut(s) 22, 25, 400, 429, 542, 566, 700, 958
BmcAI AGTACT 1 cut(s) 1040
Bme1390I CCNGG 8 cut(s) 357, 413, 448, 656, 755, 773, 774, 890
Bme18I GGWCC 4 cut(s) 634, 769, 787, 892
BmeT110I CYCGRG 1 cut(s) 772
BmgBI CACGTC 1 cut(s) 970
BmrFI CCNGG 8 cut(s) 357, 413, 448, 656, 755, 773, 774, 890
BmrI ACTGGG 1 cut(s) 833
BmsI GCATC 3 cut(s) 567, 612, 1015
BmuI ACTGGG 1 cut(s) 833
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 4 cut(s) 357, 413, 773, 774
BsaAI YACGTR 1 cut(s) 905
BsaHI GRCGYC 1 cut(s) 936
BsaI GGTCTC 1 cut(s) 656
BsaWI WCCGGW 2 cut(s) 789, 923
Bse118I RCCGGY 2 cut(s) 308, 554
Bse1I ACTGG 3 cut(s) 66, 828, 1046
Bse21I CCTNAGG 1 cut(s) 896
BseAI TCCGGA 1 cut(s) 789
BseBI CCWGG 4 cut(s) 448, 656, 755, 890
BseGI GGATG 3 cut(s) 116, 558, 1027
BseMII CTCAG 3 cut(s) 292, 734, 887
BseNI ACTGG 3 cut(s) 66, 828, 1046
BseSI GKGCMC 2 cut(s) 830, 888
BseX3I CGGCCG 1 cut(s) 699
BseXI GCAGC 2 cut(s) 414, 968
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 1 cut(s) 743
Bsh1236I CGCG 4 cut(s) 390, 564, 698, 797
Bsh1285I CGRYCG 1 cut(s) 702
BshNI GGYRCC 2 cut(s) 759, 827
BsiEI CGRYCG 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 772
BslFI GGGAC 3 cut(s) 54, 220, 782
BsmAI GTCTC 2 cut(s) 535, 656
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 3 cut(s) 54, 220, 782
Bso31I GGTCTC 1 cut(s) 656
BsoBI CYCGRG 1 cut(s) 772
Bsp120I GGGCCC 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 351, 830, 888
Bsp13I TCCGGA 1 cut(s) 789
BspCNI CTCAG 3 cut(s) 291, 735, 888
BspEI TCCGGA 1 cut(s) 789
BspFNI CGCG 4 cut(s) 390, 564, 698, 797
BspPI GGATC 3 cut(s) 412, 491, 747
BspT107I GGYRCC 2 cut(s) 759, 827
BspTNI GGTCTC 1 cut(s) 656
BsrBI CCGCTC 2 cut(s) 46, 686
BsrFI RCCGGY 2 cut(s) 308, 554
BsrI ACTGG 3 cut(s) 66, 828, 1046
BssAI RCCGGY 2 cut(s) 308, 554
BssNI GRCGYC 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 4 cut(s) 448, 656, 755, 890
Bst4CI ACNGT 2 cut(s) 193, 580
Bst6I CTCTTC 3 cut(s) 67, 275, 674
BstACI GRCGYC 1 cut(s) 936
BstBAI YACGTR 1 cut(s) 905
BstC8I GCNNGC 7 cut(s) 48, 310, 476, 556, 722, 751, 988
BstDEI CTNAG 3 cut(s) 278, 743, 896
BstDSI CCRYGG 1 cut(s) 562
BstF5I GGATG 3 cut(s) 116, 558, 1027
BstFNI CGCG 4 cut(s) 390, 564, 698, 797
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 3 cut(s) 392, 749, 956
BstMAI GTCTC 2 cut(s) 535, 656
BstMCI CGRYCG 1 cut(s) 702
BstNI CCWGG 4 cut(s) 448, 656, 755, 890
BstSCI CCNGG 8 cut(s) 355, 411, 446, 654, 753, 771, 772, 888
BstSLI GKGCMC 2 cut(s) 830, 888
BstUI CGCG 4 cut(s) 390, 564, 698, 797
BstV1I GCAGC 2 cut(s) 414, 968
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BstZI CGGCCG 1 cut(s) 699
Bsu36I CCTNAGG 1 cut(s) 896
BtgI CCRYGG 1 cut(s) 562
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 3 cut(s) 116, 558, 1027
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 7 cut(s) 48, 310, 476, 556, 722, 751, 988
CaiI CAGNNNCTG 1 cut(s) 895
CfoI GCGC 3 cut(s) 392, 749, 956
Cfr10I RCCGGY 2 cut(s) 308, 554
Cfr42I CCGCGG 1 cut(s) 565
Cfr9I CCCGGG 1 cut(s) 772
Csp6I GTAC 3 cut(s) 760, 821, 1039
CviAII CATG 4 cut(s) 343, 487, 649, 916
CviQI GTAC 3 cut(s) 760, 821, 1039
DdeI CTNAG 3 cut(s) 278, 743, 896
DraIII CACNNNGTG 1 cut(s) 919
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 2 cut(s) 3, 699
EagI CGGCCG 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 67, 275, 674
EarI CTCTTC 3 cut(s) 67, 275, 674
EciI GGCGGA 1 cut(s) 375
EclXI CGGCCG 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 1 cut(s) 888
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 4 cut(s) 634, 769, 787, 892
Eco52I CGGCCG 1 cut(s) 699
Eco72I CACGTG 1 cut(s) 905
Eco81I CCTNAGG 1 cut(s) 896
Eco88I CYCGRG 1 cut(s) 772
EcoO109I RGGNCCY 4 cut(s) 769, 849, 885, 892
EcoRII CCWGG 4 cut(s) 446, 654, 753, 888
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 888
ErhI CCWWGG 1 cut(s) 510
Esp3I CGTCTC 1 cut(s) 535
FaeI CATG 4 cut(s) 346, 490, 652, 919
FaiI YATR 5 cut(s) 344, 488, 650, 872, 917
FaqI GGGAC 3 cut(s) 54, 220, 782
FatI CATG 4 cut(s) 342, 486, 648, 915
FauI CCCGC 4 cut(s) 228, 245, 483, 559
FbaI TGATCA 2 cut(s) 645, 993
FblI GTMKAC 2 cut(s) 339, 933
Fnu4HI GCNGC 8 cut(s) 21, 24, 399, 428, 541, 565, 699, 957
FokI GGATG 3 cut(s) 103, 545, 1034
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 8 cut(s) 21, 24, 399, 428, 541, 565, 699, 957
GlaI GCGC 3 cut(s) 391, 748, 955
GluI GCNGC 8 cut(s) 21, 24, 399, 428, 541, 565, 699, 957
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 1 cut(s) 577
HaeII RGCGCY 1 cut(s) 750
HhaI GCGC 3 cut(s) 392, 749, 956
Hin1I GRCGYC 1 cut(s) 936
Hin1II CATG 4 cut(s) 346, 490, 652, 919
Hin6I GCGC 3 cut(s) 390, 747, 954
HinP1I GCGC 3 cut(s) 390, 747, 954
HincII GTYRAC 3 cut(s) 340, 715, 934
HindII GTYRAC 3 cut(s) 340, 715, 934
HinfI GANTC 3 cut(s) 13, 363, 434
HphI GGTGA 3 cut(s) 22, 33, 127
Hpy166II GTNNAC 3 cut(s) 340, 715, 934
Hpy188I TCNGA 5 cut(s) 175, 370, 587, 744, 1006
Hpy188III TCNNGA 2 cut(s) 493, 790
Hpy8I GTNNAC 3 cut(s) 340, 715, 934
Hpy99I CGWCG 9 cut(s) 335, 338, 341, 584, 611, 707, 935, 938, 941
HpyAV CCTTC 4 cut(s) 701, 778, 796, 808
HpyCH4III ACNGT 2 cut(s) 193, 580
HpyCH4IV ACGT 3 cut(s) 904, 936, 969
HpyCH4V TGCA 2 cut(s) 724, 1028
HpyF3I CTNAG 3 cut(s) 278, 743, 896
HpySE526I ACGT 3 cut(s) 904, 936, 969
Hsp92I GRCGYC 1 cut(s) 936
Hsp92II CATG 4 cut(s) 346, 490, 652, 919
HspAI GCGC 3 cut(s) 390, 747, 954
KflI GGGWCCC 1 cut(s) 769
Kpn2I TCCGGA 1 cut(s) 789
KpnI GGTACC 1 cut(s) 763
KroI GCCGGC 2 cut(s) 308, 554
KroNI GCCGGC 2 cut(s) 310, 556
Ksp22I TGATCA 2 cut(s) 645, 993
KspI CCGCGG 1 cut(s) 565
LmnI GCTCC 6 cut(s) 346, 512, 572, 951, 1012, 1044
Lsp1109I GCAGC 2 cut(s) 414, 968
LweI GCATC 3 cut(s) 567, 612, 1015
MaeII ACGT 3 cut(s) 904, 936, 969
MaeIII GTNAC 1 cut(s) 193
MbiI CCGCTC 2 cut(s) 46, 686
MboII GAAGA 3 cut(s) 54, 262, 691
MfeI CAATTG 1 cut(s) 638
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 3 cut(s) 351, 830, 888
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 625, 638
MluI ACGCGT 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 717
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 789
MroNI GCCGGC 2 cut(s) 308, 554
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 564, 959
MspR9I CCNGG 8 cut(s) 357, 413, 448, 656, 755, 773, 774, 890
MunI CAATTG 1 cut(s) 638
MvaI CCWGG 4 cut(s) 448, 656, 755, 890
MvnI CGCG 4 cut(s) 390, 564, 698, 797
NaeI GCCGGC 2 cut(s) 310, 556
NciI CCSGG 4 cut(s) 357, 413, 773, 774
NgoMIV GCCGGC 2 cut(s) 308, 554
NlaIII CATG 4 cut(s) 346, 490, 652, 919
NmeAIII GCCGAG 4 cut(s) 325, 450, 544, 835
PcsI WCGNNNNNNNCGW 4 cut(s) 330, 336, 606, 702
PdiI GCCGGC 2 cut(s) 310, 556
PfeI GAWTC 2 cut(s) 363, 434
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 8 cut(s) 22, 25, 400, 429, 542, 566, 700, 958
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 905
PmlI CACGTG 1 cut(s) 905
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 905
PpuMI RGGWCCY 2 cut(s) 769, 892
Psp5II RGGWCCY 2 cut(s) 769, 892
Psp6I CCWGG 4 cut(s) 446, 654, 753, 888
PspCI CACGTG 1 cut(s) 905
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 4 cut(s) 446, 654, 753, 888
PspOMI GGGCCC 1 cut(s) 884
PspPPI RGGWCCY 2 cut(s) 769, 892
PstNI CAGNNNCTG 1 cut(s) 895
PsuI RGATCY 1 cut(s) 739
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 3 cut(s) 761, 822, 1040
RsaNI GTAC 3 cut(s) 760, 821, 1039
SacII CCGCGG 1 cut(s) 565
SalI GTCGAC 2 cut(s) 338, 932
SatI GCNGC 8 cut(s) 21, 24, 399, 428, 541, 565, 699, 957
ScaI AGTACT 1 cut(s) 1040
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 8 cut(s) 357, 413, 448, 656, 755, 773, 774, 890
SduI GDGCHC 3 cut(s) 351, 830, 888
SfaNI GCATC 3 cut(s) 567, 612, 1015
Sfr303I CCGCGG 1 cut(s) 565
SgrBI CCGCGG 1 cut(s) 565
SgrDI CGTCGACG 1 cut(s) 932
SinI GGWCC 4 cut(s) 634, 769, 787, 892
SmaI CCCGGG 1 cut(s) 774
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 2 cut(s) 625, 638
StyD4I CCNGG 8 cut(s) 355, 411, 446, 654, 753, 771, 772, 888
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 193, 580
TaiI ACGT 3 cut(s) 907, 939, 972
TaqI TCGA 6 cut(s) 339, 465, 501, 606, 705, 933
TaqII GACCGA 1 cut(s) 680
TasI AATT 2 cut(s) 625, 638
TatI WGTACW 1 cut(s) 1038
TauI GCSGC 6 cut(s) 23, 26, 401, 543, 567, 701
TfiI GAWTC 2 cut(s) 363, 434
TscAI CASTG 1 cut(s) 15
TseI GCWGC 2 cut(s) 427, 956
TspGWI ACGGA 1 cut(s) 919
TspMI CCCGGG 1 cut(s) 772
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 329
VpaK11BI GGWCC 4 cut(s) 634, 769, 787, 892
XmaI CCCGGG 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 339, 933
ZraI GACGTC 1 cut(s) 937
ZrmI AGTACT 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.