Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000135.1

Orthogroup ID

OG0010499

Physical Location & Seq

Genomic Coordinates Reverse (-)

190224 .. 195519

Gene Structure 5296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

atgaaaacgacgtcgctttcgtccactattatgcccgttaagctcctcctcaacaacccttctctcccagtaacccgcttcctctccatgaaattcggaccctccatttccgctcctcgacccgtacccgctttctcccgggtcttcccgctccgcctcaggtacctcgccctcggctcccagcgcacattctccacccgcgccttccggaccccccgaaccgggtcgggctcccaattctcgcgcggctacgagcgaaaggcggcggtcgggggatccaagagcctggtcgacgacgaggcggagctgagtgactgggtcagcgagttgaggaccgacccgacccgcggtggcggtggccggggagaggaatcggacggtgctggtcggagaggtagtagggttagagataggggcggcgatagagagccgtatccggcgaagcggagcaggagagatagcggcgccgacgagtttggtgagtcgagaactcggggagggtttcggtcttttccgggaagttcacggatgagtaaaagggatgatcatgatgatagagaaagtaggtcatttccgccgagaagcaatcgggggagttcgagctcgaatgaccggtttggtgagtcgaatcggagacgatttccgagctcgaatccgtcggcatttactagaaatccacggatggatagaaggtttgataacaaatttggggacaatgctgtgaaggaagatgggtttacggggaggagaggtccgagtttcagagggaattcgaatgcgagttcgagttctttgactaagagaggagggagagaggtggattcgggtttcagacgaggcggcgaggggttgaggaagggtgggagagattcgaggaaggaaaaggaaatgcttgtggtggatgatagtgatgaggatgaggaggtgaatgagcttggtgttggcattggggatttgcttaccgaggaagatagtgatagtgctgcttcggatgaggatgatgagtacaaaggattgaaagataagagttcagctgcattgtttggctcggataaggaagtggtgagtcagaaggctgtgccgaaaagttcagcgggagactctgactcttatttgagcgagtcaaggtttgatcagtgttcggtctcgccactgtccttgaagggaatcaaggatgcgggatatgagaacatgactgtggtacaagaggctactcttcccatcattctcaaaggcaaggatgttctagccaaggctaaaaccggaactggaaaaactgttgcattcttgcttccatcaattgaagttgttgtaaaatcacctcctattggacgcgatcataagagacccccaatttctgtacttgtaatatgcccaacccgagagctggcgagtcaggctgcggcagaagccaataaattgttgaagtatcatccttctattggtgttcaagttgtgataggaggcaaggatgttctagccaaggctaaaaccggaactggaaaaactgttgcattcttgcttccatcaattgaagttgttgtaaaatcacctcctattggacgcgatcataagagacccccaatttctgtacttgtaatatgcccaacccgagagctggcgagtcaggctgcggcagaagccaataaattgttgaagtatcatccttctattggtgttcaagttgtgataggaggtacaagacttgctctagaacagaaacgcatgcaagcaaacccttgccagattcttgtcgctacaccgggaaggctcaaagatcacattgagaataccgcaggtttcgcaactaggctgatgggtgtcaaagtcctcattcttgatgaagctgatcatctactggatatggggttccgtaaagacattgaaaggattatagcagctgttccaaaacagcgacagacactactgttctctgccaccgttccagaagaggtccgtcaaatctgtcatatcgctttgaaaagagatcatgaatttattaacacggttcaggaaggcagtgaagagacacatgcgcaggtcagacaaacccatttggttgctcctttggacaaacacttttcctttgtatatagtcttcttaaagagcacattgctgacgatcctgactataaggttctcgtattctgcactactgcaatggtcacacgactggttgctgaccttcttggtgagctgaacttgaatgttagagaaatccactccagaaagccacagagttatagaactagggtttccgatgaatttcggaaatcaaagggtcttatccttgtgacttctgatgtatctgcacgaggggttgattatccagacgttactctagtcatacaggtgggcattccagctgatagacagcagtatattcaccgacttggtagaactggccgtaaaggcaaagaagggcaaggaatattgctactggctccttgggaggaatttttcttatccactattaaagatttgccaatgaccaaggctcctgtacctttagttgatccagatacgaccaagaaggtagcacgagctctatcccaagtggacatgaagaacaaagaagcagcataccaagcgtggctggggtattacaattcaaataagaaaatagggcgtgataagcataggcttgtggagcttgctaatgagtttagccgaagcatgggattggacaaccctccagccattgccaagcttgttcttggtaaaatgggtcttaagaatgtccctggactgcgttccaaataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

920

Amino Acids

101.1

Weight (kDa)

9.67

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	400 - 489	3.9e-18	DEAD/DEAH box helicase
DEAD	PF00270	485 - 654	4.6e-42	DEAD/DEAH box helicase
ResIII	PF04851	490 - 649	1e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	700 - 805	3.4e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63630 AT5G63630
fragaria_vesca	FvH4_7g16280
malus_domestica	MD01G1079300.v1.1 MD07G1148200.v1.1
prunus_persica	Prupe.2G186500_v2.0.a1
pyrus_communis	pycom01g10760 pycom07g14720
rosa_chinensis	RchiOBHm_Chr1g0359291
rosa_laevigata	RLG00000027883
rosa_multiflora	Rmu_co8196408.1_g000001 Rmu_ssc0000135.1_g000038
rosa_roxburghii	Rroxscaffold_4G00297140 Rroxscaffold_5G00348520
rosa_rugosa	Rorug01G0269200
rosa_samantha	Rh1AG283200 Rh1BG249400 Rh1CG267300 Rh1DG278300 Rh2AG248100
rosa_wichuraiana	Rw1G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	2037
Acc36I	ACCTGC	2 cut(s)	1787, 2029
Acc65I	GGTACC	1 cut(s)	162
AccB1I	GGYRCC	2 cut(s)	162, 464
AccBSI	CCGCTC	2 cut(s)	113, 151
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
AccIII	TCCGGA	1 cut(s)	207
AclWI	GGATC	4 cut(s)	270, 283, 2117, 2510
AcoI	YGGCCR	2 cut(s)	358, 2404
AcsI	RAATTY	6 cut(s)	92, 704, 769, 1994, 2264, 2456
AcyI	GRCGYC	2 cut(s)	11, 465
AfaI	GTAC	8 cut(s)	126, 164, 1007, 1203, 1362, 1593, 1699, 2505
AflII	CTTAAG	1 cut(s)	2732
AgeI	ACCGGT	1 cut(s)	612
AjnI	CCWGG	2 cut(s)	285, 2743
AloI	GAACNNNNNNTCC	2 cut(s)	1853, 1885
Alw21I	GWGCWC	4 cut(s)	605, 650, 2112, 2548
Alw26I	GTCTC	6 cut(s)	628, 1092, 1150, 1339, 1570, 2021
AlwI	GGATC	4 cut(s)	270, 283, 2117, 2510
Ama87I	CYCGRG	4 cut(s)	138, 492, 1380, 1611
Aor13HI	TCCGGA	1 cut(s)	207
AoxI	GGCC	2 cut(s)	358, 2404
ApeKI	GCWGC	6 cut(s)	983, 1034, 1400, 1631, 1898, 2579
ApoI	RAATTY	6 cut(s)	92, 704, 769, 1994, 2264, 2456
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AsiGI	ACCGGT	1 cut(s)	612
Asp718I	GGTACC	1 cut(s)	162
AspLEI	GCGC	5 cut(s)	186, 203, 246, 467, 2038
AspS9I	GGNCC	5 cut(s)	98, 210, 333, 752, 1954
AsuC2I	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1764
AsuHPI	GGTGA	8 cut(s)	491, 632, 937, 1075, 1311, 1542, 2204, 2378
AsuII	TTCGAA	1 cut(s)	773
AvaI	CYCGRG	4 cut(s)	138, 492, 1380, 1611
AvaII	GGWCC	5 cut(s)	98, 210, 333, 752, 1954
AxyI	CCTNAGG	1 cut(s)	158
BamHI	GGATCC	1 cut(s)	275
BanI	GGYRCC	2 cut(s)	162, 464
BanII	GRGCYC	4 cut(s)	233, 605, 650, 2548
BarI	GAAGNNNNNNTAC	2 cut(s)	2082, 2114
BauI	CACGAG	2 cut(s)	2313, 2541
BbsI	GAAGAC	2 cut(s)	136, 2090
Bbv12I	GWGCWC	4 cut(s)	605, 650, 2112, 2548
BbvI	GCAGC	6 cut(s)	970, 1021, 1387, 1618, 1910, 2591
BccI	CCATC	6 cut(s)	676, 725, 1229, 1303, 1534, 1810
BceAI	ACGGC	2 cut(s)	415, 2391
BciT130I	CCWGG	2 cut(s)	287, 2745
BciVI	GTATCC	1 cut(s)	444
BclI	TGATCA	3 cut(s)	544, 1132, 1849
BcnI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1764
BcoDI	GTCTC	6 cut(s)	628, 1092, 1150, 1339, 1570, 2021
BfaI	CTAG	7 cut(s)	669, 1247, 1478, 1712, 1809, 2250, 2342
BfoI	RGCGCY	1 cut(s)	468
BfrI	CTTAAG	1 cut(s)	2732
BfuAI	ACCTGC	2 cut(s)	1787, 2029
BfuI	GTATCC	1 cut(s)	444
BglI	GCCNNNNNGGC	1 cut(s)	2412
Bme1390I	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1764, 2745
Bme18I	GGWCC	5 cut(s)	98, 210, 333, 752, 1954
BmeT110I	CYCGRG	4 cut(s)	138, 492, 1380, 1611
BmgT120I	GGNCC	5 cut(s)	98, 210, 333, 752, 1954
BmrFI	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1764, 2745
BmrI	ACTGGG	2 cut(s)	62, 325
BmsI	GCATC	1 cut(s)	1165
BmuI	ACTGGG	2 cut(s)	62, 325
BpiI	GAAGAC	2 cut(s)	136, 2090
BpmI	CTGGAG	2 cut(s)	2209, 2679
Bpu14I	TTCGAA	1 cut(s)	773
BpuMI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1764
BsaHI	GRCGYC	2 cut(s)	11, 465
BsaI	GGTCTC	3 cut(s)	1150, 1339, 1570
BsaWI	WCCGGW	4 cut(s)	207, 612, 1262, 1493
BsaXI	ACNNNNNCTCC	6 cut(s)	382, 412, 736, 766, 2482, 2512
Bse118I	RCCGGY	1 cut(s)	612
Bse1I	ACTGG	8 cut(s)	68, 320, 1273, 1504, 1863, 2178, 2407, 2445
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	3 cut(s)	2112, 2166, 2700
BseAI	TCCGGA	1 cut(s)	207
BseBI	CCWGG	2 cut(s)	287, 2745
BseMI	GCAATG	3 cut(s)	2112, 2166, 2700
BseMII	CTCAG	2 cut(s)	172, 299
BseNI	ACTGG	8 cut(s)	68, 320, 1273, 1504, 1863, 2178, 2407, 2445
BseRI	GAGGAG	6 cut(s)	35, 38, 105, 760, 819, 935
BseXI	GCAGC	6 cut(s)	970, 1021, 1387, 1618, 1910, 2591
BseYI	CCCAGC	2 cut(s)	180, 2596
BsgI	GTGCAG	2 cut(s)	2134, 2295
Bsh1236I	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
Bsh1285I	CGRYCG	1 cut(s)	270
BshFI	GGCC	2 cut(s)	360, 2406
BshNI	GGYRCC	2 cut(s)	162, 464
BshTI	ACCGGT	1 cut(s)	612
BsiEI	CGRYCG	1 cut(s)	270
BsiHKAI	GWGCWC	4 cut(s)	605, 650, 2112, 2548
BsiHKCI	CYCGRG	4 cut(s)	138, 492, 1380, 1611
BslFI	GGGAC	2 cut(s)	725, 2726
BsmAI	GTCTC	6 cut(s)	628, 1092, 1150, 1339, 1570, 2021
BsmBI	CGTCTC	1 cut(s)	628
BsmFI	GGGAC	2 cut(s)	725, 2726
BsmI	GAATGC	4 cut(s)	781, 1283, 1514, 2358
BsnI	GGCC	2 cut(s)	360, 2406
Bso31I	GGTCTC	3 cut(s)	1150, 1339, 1570
BsoBI	CYCGRG	4 cut(s)	138, 492, 1380, 1611
Bsp119I	TTCGAA	1 cut(s)	773
Bsp1286I	GDGCHC	5 cut(s)	233, 605, 650, 2112, 2548
Bsp13I	TCCGGA	1 cut(s)	207
BspANI	GGCC	2 cut(s)	360, 2406
BspCNI	CTCAG	2 cut(s)	171, 300
BspEI	TCCGGA	1 cut(s)	207
BspFNI	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
BspHI	TCATGA	2 cut(s)	547, 1990
BspMI	ACCTGC	2 cut(s)	1787, 2029
BspPI	GGATC	4 cut(s)	270, 283, 2117, 2510
BspT104I	TTCGAA	1 cut(s)	773
BspT107I	GGYRCC	2 cut(s)	162, 464
BspTI	CTTAAG	1 cut(s)	2732
BspTNI	GGTCTC	3 cut(s)	1150, 1339, 1570
BsrBI	CCGCTC	2 cut(s)	113, 151
BsrDI	GCAATG	3 cut(s)	2112, 2166, 2700
BsrFI	RCCGGY	1 cut(s)	612
BsrI	ACTGG	8 cut(s)	68, 320, 1273, 1504, 1863, 2178, 2407, 2445
BssAI	RCCGGY	1 cut(s)	612
BssNI	GRCGYC	2 cut(s)	11, 465
BssSI	CACGAG	2 cut(s)	2313, 2541
BssT1I	CCWWGG	4 cut(s)	1251, 1482, 2447, 2493
Bst2BI	CACGAG	2 cut(s)	2313, 2541
Bst2UI	CCWGG	2 cut(s)	287, 2745
Bst4CI	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1510, 1929, 1942, 2008
Bst6I	CTCTTC	3 cut(s)	1221, 1944, 2019
BstACI	GRCGYC	2 cut(s)	11, 465
BstAFI	CTTAAG	1 cut(s)	2732
BstBI	TTCGAA	1 cut(s)	773
BstC8I	GCNNGC	5 cut(s)	1389, 1620, 1727, 1731, 2655
BstDEI	CTNAG	3 cut(s)	158, 308, 798
BstDSI	CCRYGG	2 cut(s)	346, 677
BstFNI	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
BstH2I	RGCGCY	1 cut(s)	468
BstHHI	GCGC	5 cut(s)	186, 203, 246, 467, 2038
BstMAI	GTCTC	6 cut(s)	628, 1092, 1150, 1339, 1570, 2021
BstMCI	CGRYCG	1 cut(s)	270
BstNI	CCWGG	2 cut(s)	287, 2745
BstNSI	RCATGY	2 cut(s)	1729, 2036
BstSCI	CCNGG	8 cut(s)	137, 138, 221, 285, 360, 514, 1762, 2743
BstUI	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
BstV1I	GCAGC	6 cut(s)	970, 1021, 1387, 1618, 1910, 2591
BstV2I	GAAGAC	2 cut(s)	136, 2090
BstX2I	RGATCY	1 cut(s)	275
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	275
Bsu36I	CCTNAGG	1 cut(s)	158
BsuI	GTATCC	1 cut(s)	444
BsuRI	GGCC	2 cut(s)	360, 2406
BtgI	CCRYGG	2 cut(s)	346, 677
BtsI	GCAGTG	1 cut(s)	2026
BtsIMutI	CAGTG	3 cut(s)	1142, 1151, 2026
BveI	ACCTGC	2 cut(s)	1787, 2029
Cac8I	GCNNGC	5 cut(s)	1389, 1620, 1727, 1731, 2655
CciI	TCATGA	2 cut(s)	547, 1990
CfoI	GCGC	5 cut(s)	186, 203, 246, 467, 2038
Cfr10I	RCCGGY	1 cut(s)	612
Cfr13I	GGNCC	5 cut(s)	98, 210, 333, 752, 1954
Cfr42I	CCGCGG	1 cut(s)	349
Cfr9I	CCCGGG	1 cut(s)	138
CseI	GACGC	2 cut(s)	1341, 1572
Csp6I	GTAC	8 cut(s)	125, 163, 1006, 1202, 1361, 1592, 1698, 2504
CspAI	ACCGGT	1 cut(s)	612
CspCI	CAANNNNNGTGG	2 cut(s)	1140, 1175
CviAII	CATG	8 cut(s)	88, 548, 1192, 1726, 1991, 2033, 2563, 2677
CviQI	GTAC	8 cut(s)	125, 163, 1006, 1202, 1361, 1592, 1698, 2504
DdeI	CTNAG	3 cut(s)	158, 308, 798
DinI	GGCGCC	1 cut(s)	466
EaeI	YGGCCR	2 cut(s)	358, 2404
Eam1104I	CTCTTC	3 cut(s)	1221, 1944, 2019
EarI	CTCTTC	3 cut(s)	1221, 1944, 2019
EciI	GGCGGA	3 cut(s)	143, 317, 564
Ecl136II	GAGCTC	3 cut(s)	603, 648, 2546
Eco130I	CCWWGG	4 cut(s)	1251, 1482, 2447, 2493
Eco24I	GRGCYC	4 cut(s)	233, 605, 650, 2548
Eco31I	GGTCTC	3 cut(s)	1150, 1339, 1570
Eco47I	GGWCC	5 cut(s)	98, 210, 333, 752, 1954
Eco53kI	GAGCTC	3 cut(s)	603, 648, 2546
Eco81I	CCTNAGG	1 cut(s)	158
Eco88I	CYCGRG	4 cut(s)	138, 492, 1380, 1611
EcoICRI	GAGCTC	3 cut(s)	603, 648, 2546
EcoRI	GAATTC	1 cut(s)	769
EcoRII	CCWGG	2 cut(s)	285, 2743
EcoT14I	CCWWGG	4 cut(s)	1251, 1482, 2447, 2493
EcoT38I	GRGCYC	4 cut(s)	233, 605, 650, 2548
EgeI	GGCGCC	1 cut(s)	466
EheI	GGCGCC	1 cut(s)	466
ErhI	CCWWGG	4 cut(s)	1251, 1482, 2447, 2493
Esp3I	CGTCTC	1 cut(s)	628
FaeI	CATG	8 cut(s)	91, 551, 1195, 1729, 1994, 2036, 2566, 2680
FaqI	GGGAC	2 cut(s)	725, 2726
FatI	CATG	8 cut(s)	87, 547, 1191, 1725, 1990, 2032, 2562, 2676
FauI	CCCGC	7 cut(s)	83, 136, 156, 206, 353, 1087, 1171
FbaI	TGATCA	3 cut(s)	544, 1132, 1849
FblI	GTMKAC	1 cut(s)	291
FriOI	GRGCYC	4 cut(s)	233, 605, 650, 2548
FspBI	CTAG	7 cut(s)	669, 1247, 1478, 1712, 1809, 2250, 2342
FspI	TGCGCA	1 cut(s)	2037
GlaI	GCGC	5 cut(s)	185, 202, 245, 466, 2037
GsaI	CCCAGC	2 cut(s)	184, 2600
GsuI	CTGGAG	2 cut(s)	2209, 2679
HaeII	RGCGCY	1 cut(s)	468
HaeIII	GGCC	2 cut(s)	360, 2406
HgaI	GACGC	2 cut(s)	1341, 1572
HhaI	GCGC	5 cut(s)	186, 203, 246, 467, 2038
Hin1I	GRCGYC	2 cut(s)	11, 465
Hin1II	CATG	8 cut(s)	91, 551, 1195, 1729, 1994, 2036, 2566, 2680
Hin6I	GCGC	5 cut(s)	184, 201, 244, 465, 2036
HinP1I	GCGC	5 cut(s)	184, 201, 244, 465, 2036
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	2708
HphI	GGTGA	8 cut(s)	491, 632, 937, 1075, 1311, 1542, 2204, 2378
Hpy166II	GTNNAC	5 cut(s)	24, 292, 524, 738, 2560
Hpy8I	GTNNAC	5 cut(s)	24, 292, 524, 738, 2560
Hpy99I	CGWCG	6 cut(s)	13, 16, 296, 299, 473, 661
HpyCH4III	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1510, 1929, 1942, 2008
HpyCH4IV	ACGT	2 cut(s)	11, 2334
HpyCH4V	TGCA	7 cut(s)	1037, 1283, 1514, 1729, 2151, 2159, 2312
HpyF3I	CTNAG	3 cut(s)	158, 308, 798
HpySE526I	ACGT	2 cut(s)	11, 2334
Hsp92I	GRCGYC	2 cut(s)	11, 465
Hsp92II	CATG	8 cut(s)	91, 551, 1195, 1729, 1994, 2036, 2566, 2680
HspAI	GCGC	5 cut(s)	184, 201, 244, 465, 2036
KasI	GGCGCC	1 cut(s)	464
Kpn2I	TCCGGA	1 cut(s)	207
KpnI	GGTACC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	544, 1132, 1849
KspI	CCGCGG	1 cut(s)	349
Lsp1109I	GCAGC	6 cut(s)	970, 1021, 1387, 1618, 1910, 2591
LweI	GCATC	1 cut(s)	1165
MaeI	CTAG	7 cut(s)	669, 1247, 1478, 1712, 1809, 2250, 2342
MaeII	ACGT	2 cut(s)	11, 2334
MaeIII	GTNAC	5 cut(s)	70, 311, 2164, 2293, 2335
MbiI	CCGCTC	2 cut(s)	113, 151
MboII	GAAGA	8 cut(s)	136, 740, 980, 1208, 1961, 2036, 2090, 2578
MfeI	CAATTG	2 cut(s)	1299, 1530
MflI	RGATCY	1 cut(s)	275
MhlI	GDGCHC	5 cut(s)	233, 605, 650, 2112, 2548
Mly113I	GGCGCC	1 cut(s)	465
MlyI	GAGTC	8 cut(s)	491, 632, 1075, 1094, 1100, 1130, 1402, 1633
MmeI	TCCRAC	1 cut(s)	368
MroI	TCCGGA	1 cut(s)	207
MseI	TTAA	5 cut(s)	39, 2001, 2103, 2475, 2733
MslI	CAYNNNNRTG	3 cut(s)	29, 1196, 2351
MspA1I	CMGCKG	5 cut(s)	348, 1034, 1094, 1901, 2366
MspCI	CTTAAG	1 cut(s)	2732
MspR9I	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1764, 2745
MunI	CAATTG	2 cut(s)	1299, 1530
Mva1269I	GAATGC	4 cut(s)	781, 1283, 1514, 2358
MvaI	CCWGG	2 cut(s)	287, 2745
MvnI	CGCG	6 cut(s)	201, 244, 246, 348, 1335, 1566
NarI	GGCGCC	1 cut(s)	465
NciI	CCSGG	6 cut(s)	139, 140, 223, 362, 516, 1764
NlaIII	CATG	8 cut(s)	91, 551, 1195, 1729, 1994, 2036, 2566, 2680
NmeAIII	GCCGAG	2 cut(s)	153, 603
NmuCI	GTSAC	3 cut(s)	311, 2164, 2293
NsbI	TGCGCA	1 cut(s)	2037
NspI	RCATGY	2 cut(s)	1729, 2036
NspV	TTCGAA	1 cut(s)	773
PaeI	GCATGC	1 cut(s)	1729
PagI	TCATGA	2 cut(s)	547, 1990
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	4 cut(s)	781, 1283, 1514, 2358
PfeI	GAWTC	7 cut(s)	371, 628, 652, 821, 869, 1167, 1747
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	1 cut(s)	514
PinAI	ACCGGT	1 cut(s)	612
PleI	GAGTC	8 cut(s)	490, 631, 1074, 1094, 1100, 1129, 1401, 1632
PluTI	GGCGCC	1 cut(s)	468
PpsI	GAGTC	8 cut(s)	490, 631, 1074, 1094, 1100, 1129, 1401, 1632
Psp124BI	GAGCTC	3 cut(s)	605, 650, 2548
Psp6I	CCWGG	2 cut(s)	285, 2743
PspFI	CCCAGC	2 cut(s)	180, 2596
PspGI	CCWGG	2 cut(s)	285, 2743
PspPI	GGNCC	5 cut(s)	98, 210, 333, 752, 1954
PsuI	RGATCY	1 cut(s)	275
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	3 cut(s)	1034, 1901, 2366
RsaI	GTAC	8 cut(s)	126, 164, 1007, 1203, 1362, 1593, 1699, 2505
RsaNI	GTAC	8 cut(s)	125, 163, 1006, 1202, 1361, 1592, 1698, 2504
RseI	CAYNNNNRTG	3 cut(s)	29, 1196, 2351
SacI	GAGCTC	3 cut(s)	605, 650, 2548
SacII	CCGCGG	1 cut(s)	349
SalI	GTCGAC	1 cut(s)	290
SaqAI	TTAA	5 cut(s)	39, 2001, 2103, 2475, 2733
Sau96I	GGNCC	5 cut(s)	98, 210, 333, 752, 1954
SchI	GAGTC	8 cut(s)	491, 632, 1075, 1094, 1100, 1130, 1402, 1633
ScrFI	CCNGG	8 cut(s)	139, 140, 223, 287, 362, 516, 1764, 2745
SduI	GDGCHC	5 cut(s)	233, 605, 650, 2112, 2548
SfaNI	GCATC	1 cut(s)	1165
SfoI	GGCGCC	1 cut(s)	466
Sfr303I	CCGCGG	1 cut(s)	349
SfuI	TTCGAA	1 cut(s)	773
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	5 cut(s)	98, 210, 333, 752, 1954
SmaI	CCCGGG	1 cut(s)	140
SmiMI	CAYNNNNRTG	3 cut(s)	29, 1196, 2351
SmlI	CTYRAG	1 cut(s)	2732
SmoI	CTYRAG	1 cut(s)	2732
SphI	GCATGC	1 cut(s)	1729
SspDI	GGCGCC	1 cut(s)	464
SspI	AATATT	1 cut(s)	2433
SspMI	CTAG	7 cut(s)	669, 1247, 1478, 1712, 1809, 2250, 2342
SstI	GAGCTC	3 cut(s)	605, 650, 2548
StyD4I	CCNGG	8 cut(s)	137, 138, 221, 285, 360, 514, 1762, 2743
StyI	CCWWGG	4 cut(s)	1251, 1482, 2447, 2493
TaaI	ACNGT	8 cut(s)	380, 1155, 1198, 1279, 1510, 1929, 1942, 2008
TaiI	ACGT	2 cut(s)	14, 2337
TaqII	GACCGA	3 cut(s)	350, 495, 1132
TatI	WGTACW	3 cut(s)	1005, 1360, 1591
TauI	GCSGC	7 cut(s)	249, 266, 420, 465, 843, 1406, 1637
TfiI	GAWTC	7 cut(s)	371, 628, 652, 821, 869, 1167, 1747
Tru1I	TTAA	5 cut(s)	39, 2001, 2103, 2475, 2733
Tru9I	TTAA	5 cut(s)	39, 2001, 2103, 2475, 2733
TscAI	CASTG	3 cut(s)	1142, 1158, 2026
TseFI	GTSAC	3 cut(s)	311, 2164, 2293
TseI	GCWGC	6 cut(s)	983, 1034, 1400, 1631, 1898, 2579
Tsp45I	GTSAC	3 cut(s)	311, 2164, 2293
TspDTI	ATGAA	6 cut(s)	17, 104, 1857, 2007, 2277, 2579
TspGWI	ACGGA	5 cut(s)	541, 645, 694, 1862, 1946
TspMI	CCCGGG	1 cut(s)	138
TspRI	CASTG	3 cut(s)	1142, 1158, 2026
Tth111I	GACNNNGTC	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	2732
VpaK11BI	GGWCC	5 cut(s)	98, 210, 333, 752, 1954
XapI	RAATTY	6 cut(s)	92, 704, 769, 1994, 2264, 2456
XbaI	TCTAGA	1 cut(s)	1711
XceI	RCATGY	2 cut(s)	1729, 2036
XmaI	CCCGGG	1 cut(s)	138
XmiI	GTMKAC	1 cut(s)	291
XspI	CTAG	7 cut(s)	669, 1247, 1478, 1712, 1809, 2250, 2342
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.