Rmu_ssc0000155.1_g000023

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000155.1
OG0013721
Physical Location & Seq
Reverse (-)
99084 .. 100157
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
atgcttctagtcccaagcgactacgatgaatcacgtcaggaatggcgttatggaaagaagaacagacgaccaaagcaccaaggattactgtacagcattttggaacgaagaatctacagccatgtgcagagtcttctgctcccatcagtaagtaaaccaagacgtggatgtagcagatactatcgtggatgtggtcatggttgggtggccatgatagacatggatgagtttagaagggaagtgattataactctcgtcaaccccttcacaactgcaatgttgcagcgtttccctccattggaaatctatggctggggtatcccaaaagttatcttgtccgaagatccgactttgaatcaagacaattatgtggttgtagctttatctgattatgactccttggatttcatcaaaggaagacaagattggtatcatatcgaatgcaaaccgtacctcgcagagtttgaacattgcaaatttactgatgctatattttacaggggccaaatctatatggctgataattgttgcggaataattgcggtgttcgattacaaaagcaaacaacaaatgtacttggaaaagttatgcacaccatttcaaaatttatcacaacgagctgaacgagcatatcttgtggaatcaatcaagggagacttgttgtatattttaagatttacaacattaaagagtttagacgaaggccatgtgaccgagaattttagggtatataagatggtgctcaatggatccattgtggagcatgttgagataaagagcattggagacgaggctttgtttgttggtgacagtcattcaatatctttattggcttcaagctcttcaggatcgtgtcaaccaaattcgatttactttacagatgattcgataaatttttgcctatatttagataactacggcggactacaagtcctcgacaagaatattgaagtacaagttgatatgggcatcttccatttagaggatggaactattagccaatattacccaaaatattcctttgacagtaagttgctaccacccgctttttggattataccaccggccgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

357

Amino Acids

41.3

Weight (kDa)

5.75

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 55 - 302 1.2e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 164
AciI CCGC 4 cut(s) 531, 542, 921, 1044
AclWI GGATC 4 cut(s) 338, 744, 757, 856
AcoI YGGCCR 2 cut(s) 207, 1065
AcsI RAATTY 5 cut(s) 476, 604, 718, 862, 892
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 4 cut(s) 92, 452, 575, 954
AfiI CCNNNNNNNGG 4 cut(s) 164, 298, 982, 1050
AgsI TTSAA 6 cut(s) 355, 467, 602, 819, 837, 950
AhdI GACNNNNNGTC 1 cut(s) 929
AjiI CACGTC 2 cut(s) 35, 164
AjuI GAANNNNNNNTTGG 2 cut(s) 409, 441
AluBI AGCT 3 cut(s) 380, 620, 840
AluI AGCT 3 cut(s) 380, 620, 840
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 2 cut(s) 648, 780
AlwI GGATC 4 cut(s) 338, 744, 757, 856
AoxI GGCC 4 cut(s) 207, 502, 703, 1065
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 5 cut(s) 476, 604, 718, 862, 892
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 818
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BalI TGGCCA 1 cut(s) 209
BamHI GGATCC 1 cut(s) 749
BbsI GAAGAC 2 cut(s) 125, 424
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 1 cut(s) 295
BccI CCATC 3 cut(s) 151, 730, 980
BceAI ACGGC 2 cut(s) 934, 1052
BciVI GTATCC 1 cut(s) 329
BcoDI GTCTC 2 cut(s) 648, 780
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 115
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
BmeRI GACNNNNNGTC 1 cut(s) 929
BmgBI CACGTC 2 cut(s) 35, 164
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 503, 751
BmsI GCATC 2 cut(s) 475, 978
BpiI GAAGAC 2 cut(s) 125, 424
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 79, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 298, 982, 1050
Bse118I RCCGGY 1 cut(s) 1063
Bse3DI GCAATG 2 cut(s) 282, 469
Bse8I GATNNNNATC 1 cut(s) 429
BseDI CCNNGG 2 cut(s) 79, 399
BseGI GGATG 4 cut(s) 173, 194, 229, 991
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 4 cut(s) 164, 298, 982, 1050
BseMI GCAATG 2 cut(s) 282, 469
BseX3I CGGCCG 1 cut(s) 1065
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 1068
BshFI GGCC 4 cut(s) 209, 504, 705, 1067
BsiEI CGRYCG 1 cut(s) 1068
BsiHKAI GWGCWC 1 cut(s) 744
BsiSI CCGG 1 cut(s) 1064
BslI CCNNNNNNNGG 4 cut(s) 164, 298, 982, 1050
BsmAI GTCTC 2 cut(s) 648, 780
BsmBI CGTCTC 1 cut(s) 780
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 4 cut(s) 209, 504, 705, 1067
Bsp1286I GDGCHC 1 cut(s) 744
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 3 cut(s) 343, 749, 848
BspACI CCGC 4 cut(s) 531, 542, 921, 1044
BspANI GGCC 4 cut(s) 209, 504, 705, 1067
BspLI GGNNCC 2 cut(s) 503, 751
BspPI GGATC 4 cut(s) 338, 744, 757, 856
BspQI GCTCTTC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 282, 469
BsrFI RCCGGY 1 cut(s) 1063
BsrGI TGTACA 1 cut(s) 90
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 2 cut(s) 79, 399
BssMI GATC 3 cut(s) 343, 749, 848
BssT1I CCWWGG 2 cut(s) 79, 399
Bst4CI ACNGT 4 cut(s) 90, 450, 812, 1028
Bst6I CTCTTC 1 cut(s) 847
BstAUI TGTACA 1 cut(s) 90
BstF5I GGATG 4 cut(s) 173, 194, 229, 991
BstKTI GATC 3 cut(s) 346, 752, 851
BstMAI GTCTC 2 cut(s) 648, 780
BstMBI GATC 3 cut(s) 343, 749, 848
BstMCI CGRYCG 1 cut(s) 1068
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 767
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 295
BstV2I GAAGAC 2 cut(s) 125, 424
BstX2I RGATCY 2 cut(s) 343, 749
BstYI RGATCY 2 cut(s) 343, 749
BstZI CGGCCG 1 cut(s) 1065
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 4 cut(s) 209, 504, 705, 1067
BtrI CACGTC 2 cut(s) 35, 164
BtsCI GGATG 4 cut(s) 173, 194, 229, 991
Cfr10I RCCGGY 1 cut(s) 1063
Cfr13I GGNCC 1 cut(s) 502
Csp6I GTAC 4 cut(s) 91, 451, 574, 953
CviAII CATG 6 cut(s) 122, 197, 211, 220, 707, 764
CviQI GTAC 4 cut(s) 91, 451, 574, 953
DpnI GATC 3 cut(s) 345, 751, 850
DpnII GATC 3 cut(s) 343, 749, 848
DriI GACNNNNNGTC 1 cut(s) 929
EaeI YGGCCR 2 cut(s) 207, 1065
EagI CGGCCG 1 cut(s) 1065
Eam1104I CTCTTC 1 cut(s) 847
Eam1105I GACNNNNNGTC 1 cut(s) 929
EarI CTCTTC 1 cut(s) 847
EciI GGCGGA 1 cut(s) 936
EclXI CGGCCG 1 cut(s) 1065
Eco130I CCWWGG 2 cut(s) 79, 399
Eco52I CGGCCG 1 cut(s) 1065
Eco57I CTGAAG 1 cut(s) 828
EcoT14I CCWWGG 2 cut(s) 79, 399
ErhI CCWWGG 2 cut(s) 79, 399
Esp3I CGTCTC 1 cut(s) 780
FaeI CATG 6 cut(s) 125, 200, 214, 223, 710, 767
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FatI CATG 6 cut(s) 121, 196, 210, 219, 706, 763
FauI CCCGC 1 cut(s) 1051
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 4 cut(s) 180, 201, 236, 998
Fsp4HI GCNGC 1 cut(s) 284
FspBI CTAG 1 cut(s) 8
GluI GCNGC 1 cut(s) 284
GsaI CCCAGC 1 cut(s) 316
HaeIII GGCC 4 cut(s) 209, 504, 705, 1067
HapII CCGG 1 cut(s) 1064
Hin1II CATG 6 cut(s) 125, 200, 214, 223, 710, 767
HincII GTYRAC 2 cut(s) 259, 857
HindII GTYRAC 2 cut(s) 259, 857
HinfI GANTC 7 cut(s) 29, 111, 130, 355, 395, 641, 884
HpaII CCGG 1 cut(s) 1064
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 3 cut(s) 155, 259, 857
Hpy188I TCNGA 3 cut(s) 340, 348, 388
Hpy188III TCNNGA 3 cut(s) 38, 359, 846
Hpy8I GTNNAC 3 cut(s) 155, 259, 857
HpyAV CCTTC 3 cut(s) 228, 274, 695
HpyCH4III ACNGT 4 cut(s) 90, 450, 812, 1028
HpyCH4IV ACGT 2 cut(s) 34, 163
HpyCH4V TGCA 6 cut(s) 127, 275, 283, 444, 474, 591
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpySE526I ACGT 2 cut(s) 34, 163
Hsp92II CATG 6 cut(s) 125, 200, 214, 223, 710, 767
Kzo9I GATC 3 cut(s) 343, 749, 848
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 2 cut(s) 144, 760
LpnPI CCDG 4 cut(s) 23, 298, 484, 831
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 475, 978
MaeI CTAG 1 cut(s) 8
MaeII ACGT 2 cut(s) 34, 163
MaeIII GTNAC 2 cut(s) 709, 806
MalI GATC 3 cut(s) 345, 751, 850
MboI GATC 3 cut(s) 343, 749, 848
MboII GAAGA 7 cut(s) 70, 120, 125, 353, 429, 834, 964
MflI RGATCY 2 cut(s) 343, 749
MhlI GDGCHC 1 cut(s) 744
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 8 cut(s) 364, 476, 523, 537, 604, 718, 862, 892
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 139, 389
MmeI TCCRAC 1 cut(s) 371
MnlI CCTC 5 cut(s) 303, 464, 784, 944, 976
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MseI TTAA 3 cut(s) 671, 686, 1072
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 1 cut(s) 1064
Mva1269I GAATGC 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 626
NdeII GATC 3 cut(s) 343, 749, 848
NlaIII CATG 6 cut(s) 125, 200, 214, 223, 710, 767
NlaIV GGNNCC 2 cut(s) 503, 751
NmuCI GTSAC 2 cut(s) 709, 806
NspI RCATGY 1 cut(s) 767
PciSI GCTCTTC 1 cut(s) 847
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 5 cut(s) 29, 111, 355, 641, 884
PflMI CCANNNNNTGG 1 cut(s) 164
PkrI GCNGC 1 cut(s) 285
PleI GAGTC 2 cut(s) 138, 389
PpsI GAGTC 2 cut(s) 138, 389
PsiI TTATAA 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 503, 751
PspPI GGNCC 1 cut(s) 502
PsuI RGATCY 2 cut(s) 343, 749
RsaI GTAC 4 cut(s) 92, 452, 575, 954
RsaNI GTAC 4 cut(s) 91, 451, 574, 953
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 3 cut(s) 671, 686, 1072
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 3 cut(s) 343, 749, 848
Sau96I GGNCC 1 cut(s) 502
SchI GAGTC 2 cut(s) 139, 389
SduI GDGCHC 1 cut(s) 744
SetI ASST 6 cut(s) 37, 166, 382, 456, 622, 842
SfaNI GCATC 2 cut(s) 475, 978
SfcI CTRYAG 1 cut(s) 115
Sse9I AATT 8 cut(s) 364, 476, 523, 537, 604, 718, 862, 892
SsiI CCGC 4 cut(s) 531, 542, 921, 1044
SspI AATATT 3 cut(s) 946, 1004, 1016
SspMI CTAG 1 cut(s) 8
StyI CCWWGG 2 cut(s) 79, 399
TaaI ACNGT 4 cut(s) 90, 450, 812, 1028
TaiI ACGT 2 cut(s) 37, 166
TaqI TCGA 5 cut(s) 438, 549, 866, 887, 936
TaqII GACCGA 1 cut(s) 728
TasI AATT 8 cut(s) 364, 476, 523, 537, 604, 718, 862, 892
TatI WGTACW 3 cut(s) 90, 573, 952
TfiI GAWTC 5 cut(s) 29, 111, 355, 641, 884
Tru1I TTAA 3 cut(s) 671, 686, 1072
Tru9I TTAA 3 cut(s) 671, 686, 1072
TseFI GTSAC 2 cut(s) 709, 806
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 2 cut(s) 709, 806
TspDTI ATGAA 2 cut(s) 42, 397
Van91I CCANNNNNTGG 1 cut(s) 164
XapI RAATTY 5 cut(s) 476, 604, 718, 862, 892
XceI RCATGY 1 cut(s) 767
XcmI CCANNNNNNNNNTGG 3 cut(s) 217, 983, 1047
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.