Rmu_ssc0000175.1_g000004

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000175.1
OG0016368
Physical Location & Seq
Reverse (-)
12968 .. 14176
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
atgtgcacttgttattttgttgccggacagagatgcacaaattccgcgaagaggaggcaatgtacttcttcagcccaagggatcggaagtatccgaatgaaggcaactggtaaagacttggaaattttcagtcaaaggcggaaggtaattggaaccaggaagaccctggtcctttatgatctgaaatcaaaacatggagcaaaaaagaccaattggattatgcatgaatacaaaatcgaggaaaacatttcaccatccacaagcactactagagctcctggggacaacaggttggatgattgggttttgtgcaaggtgtatctaagtaagatgtctaaacctaaaccaaatactggggatggagggaaaacagaggtgacagattcagagccattagagcaggatcttccggtgcttgaaagccctgcgactccaaacctaggtgaacaagttaacatgagcaatgaaagtgtgcaagcattatacaatggaatcatgacagtaccatatggctgtgctactggttatgtgtctcaagaggtgacaaatataggtgcaaggtcgcttgctcatcatcaacaaaacctgtcattgctaccaagcttttcgcagcttccagttattaacagtgttagctctgctatttccagccttgctggaggagacaacaacaatggaacgatcactacaccaaatgatggttcaaatggatctgaaaaggtgataaatttgcataaaaaattgcatgcagatcatcagcaagaccttccatcacttcaaaactattcgaagccctctgttattaacagtgttgggtctgctattgccagtcttgccggcggtccagtgtttcctgatttccaacaggttcctcgtggttttggagctcaatttgggacaaatatagcttccataaatggaatccaagcacatcagtcccctcatggaattggacctcatcaatctcggctaagtttggacaatggctgcctccctccattaccgcagtgttattactattatgatgatcgagaatgtagttccttttga

Protein Analysis

349

Amino Acids

37.79

Weight (kDa)

8.3

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 4 - 79 2.6e-09 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 353, 698
AccII CGCG 1 cut(s) 47
AciI CCGC 4 cut(s) 45, 139, 840, 1004
AclWI GGATC 3 cut(s) 89, 411, 718
AcsI RAATTY 3 cut(s) 40, 123, 727
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 2 cut(s) 64, 504
AfiI CCNNNNNNNGG 5 cut(s) 51, 100, 353, 440, 698
AgsI TTSAA 3 cut(s) 419, 705, 779
AjnI CCWGG 3 cut(s) 155, 165, 277
AjuI GAANNNNNNNTTGG 2 cut(s) 685, 717
AluBI AGCT 6 cut(s) 275, 603, 613, 636, 887, 908
AluI AGCT 6 cut(s) 275, 603, 613, 636, 887, 908
Alw21I GWGCWC 3 cut(s) 8, 277, 889
Alw26I GTCTC 2 cut(s) 537, 657
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 3 cut(s) 89, 411, 718
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 2 cut(s) 610, 987
ApoI RAATTY 3 cut(s) 40, 123, 727
AspA2I CCTAGG 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 169, 842, 953
AsuHPI GGTGA 5 cut(s) 243, 388, 455, 553, 733
AsuII TTCGAA 1 cut(s) 788
AvaII GGWCC 3 cut(s) 169, 842, 953
AvrII CCTAGG 1 cut(s) 439
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 277, 889
BauI CACGAG 1 cut(s) 873
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 3 cut(s) 8, 277, 889
BbvI GCAGC 2 cut(s) 622, 974
BccI CCATC 4 cut(s) 262, 353, 692, 778
BciT130I CCWGG 3 cut(s) 157, 167, 279
BciVI GTATCC 1 cut(s) 101
BcoDI GTCTC 2 cut(s) 537, 657
BfaI CTAG 2 cut(s) 270, 440
BfuI GTATCC 1 cut(s) 101
BisI GCNGC 2 cut(s) 611, 988
BlnI CCTAGG 1 cut(s) 439
BlsI GCNGC 2 cut(s) 612, 989
Bme1390I CCNGG 3 cut(s) 157, 167, 279
Bme18I GGWCC 3 cut(s) 169, 842, 953
BmgT120I GGNCC 3 cut(s) 169, 842, 953
BmiI GGNNCC 2 cut(s) 154, 870
BmrFI CCNGG 3 cut(s) 157, 167, 279
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 363
BoxI GACNNNNGTC 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 788
BpuEI CTTGAG 1 cut(s) 519
BsaJI CCNNGG 4 cut(s) 76, 165, 278, 439
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 259, 289
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 100, 353, 440, 698
Bse118I RCCGGY 1 cut(s) 836
Bse1I ACTGG 6 cut(s) 112, 358, 526, 617, 828, 845
Bse3DI GCAATG 3 cut(s) 65, 469, 590
BseBI CCWGG 3 cut(s) 157, 167, 279
BseDI CCNNGG 4 cut(s) 76, 165, 278, 439
BseGI GGATG 3 cut(s) 254, 301, 364
BseLI CCNNNNNNNGG 5 cut(s) 51, 100, 353, 440, 698
BseMI GCAATG 3 cut(s) 65, 469, 590
BseNI ACTGG 6 cut(s) 112, 358, 526, 617, 828, 845
BseRI GAGGAG 2 cut(s) 67, 675
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 622, 974
Bsh1236I CGCG 1 cut(s) 47
BsiHKAI GWGCWC 3 cut(s) 8, 277, 889
BsiSI CCGG 3 cut(s) 24, 410, 837
BslFI GGGAC 3 cut(s) 296, 910, 922
BslI CCNNNNNNNGG 5 cut(s) 51, 100, 353, 440, 698
BsmAI GTCTC 2 cut(s) 537, 657
BsmFI GGGAC 3 cut(s) 296, 910, 922
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 8, 277, 889
Bsp143I GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
BspACI CCGC 4 cut(s) 45, 139, 840, 1004
BspFNI CGCG 1 cut(s) 47
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 2 cut(s) 154, 870
BspPI GGATC 3 cut(s) 89, 411, 718
BspT104I TTCGAA 1 cut(s) 788
BsrDI GCAATG 3 cut(s) 65, 469, 590
BsrFI RCCGGY 1 cut(s) 836
BsrI ACTGG 6 cut(s) 112, 358, 526, 617, 828, 845
BssAI RCCGGY 1 cut(s) 836
BssECI CCNNGG 4 cut(s) 76, 165, 278, 439
BssMI GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
BssSI CACGAG 1 cut(s) 873
BssT1I CCWWGG 2 cut(s) 76, 439
Bst2BI CACGAG 1 cut(s) 873
Bst2UI CCWGG 3 cut(s) 157, 167, 279
Bst4CI ACNGT 3 cut(s) 502, 629, 809
Bst6I CTCTTC 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 4 cut(s) 477, 567, 747, 838
BstDEI CTNAG 2 cut(s) 323, 971
BstF5I GGATG 3 cut(s) 254, 301, 364
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 7 cut(s) 84, 181, 406, 684, 713, 754, 1030
BstMAI GTCTC 2 cut(s) 537, 657
BstMBI GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
BstMWI GCNNNNNNNGC 2 cut(s) 397, 833
BstNI CCWGG 3 cut(s) 157, 167, 279
BstNSI RCATGY 1 cut(s) 749
BstPAI GACNNNNGTC 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 155, 165, 277
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 622, 974
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 2 cut(s) 403, 710
BstYI RGATCY 2 cut(s) 403, 710
BsuI GTATCC 1 cut(s) 101
BtsCI GGATG 3 cut(s) 254, 301, 364
BtsI GCAGTG 1 cut(s) 1013
BtsIMutI CAGTG 4 cut(s) 634, 814, 852, 1013
Cac8I GCNNGC 4 cut(s) 477, 567, 747, 838
CciI TCATGA 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 836
Cfr13I GGNCC 3 cut(s) 169, 842, 953
Csp6I GTAC 2 cut(s) 63, 503
CviAII CATG 6 cut(s) 194, 224, 457, 496, 746, 944
CviQI GTAC 2 cut(s) 63, 503
DdeI CTNAG 2 cut(s) 323, 971
DpnI GATC 7 cut(s) 83, 180, 405, 683, 712, 753, 1029
DpnII GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 2 cut(s) 275, 887
Eco130I CCWWGG 2 cut(s) 76, 439
Eco24I GRGCYC 2 cut(s) 277, 889
Eco47I GGWCC 3 cut(s) 169, 842, 953
Eco53kI GAGCTC 2 cut(s) 275, 887
Eco57I CTGAAG 1 cut(s) 54
EcoICRI GAGCTC 2 cut(s) 275, 887
EcoRII CCWGG 3 cut(s) 155, 165, 277
EcoT14I CCWWGG 2 cut(s) 76, 439
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 277, 889
ErhI CCWWGG 2 cut(s) 76, 439
FaeI CATG 6 cut(s) 197, 227, 460, 499, 749, 947
FaqI GGGAC 3 cut(s) 296, 910, 922
FatI CATG 6 cut(s) 193, 223, 456, 495, 745, 943
FauNDI CATATG 1 cut(s) 508
Fnu4HI GCNGC 2 cut(s) 611, 988
FokI GGATG 3 cut(s) 241, 308, 371
FriOI GRGCYC 2 cut(s) 277, 889
Fsp4HI GCNGC 2 cut(s) 611, 988
FspBI CTAG 2 cut(s) 270, 440
GluI GCNGC 2 cut(s) 611, 988
GsuI CTGGAG 1 cut(s) 678
HapII CCGG 3 cut(s) 24, 410, 837
Hin1II CATG 6 cut(s) 197, 227, 460, 499, 749, 947
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 4 cut(s) 383, 430, 492, 921
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 3 cut(s) 24, 410, 837
HphI GGTGA 5 cut(s) 243, 388, 455, 553, 733
Hpy166II GTNNAC 3 cut(s) 6, 446, 454
Hpy188I TCNGA 5 cut(s) 86, 95, 183, 388, 715
Hpy188III TCNNGA 4 cut(s) 496, 536, 854, 1031
Hpy8I GTNNAC 3 cut(s) 6, 446, 454
HpyAV CCTTC 3 cut(s) 94, 136, 776
HpyCH4III ACNGT 3 cut(s) 502, 629, 809
HpyCH4V TGCA 9 cut(s) 6, 36, 223, 312, 475, 557, 733, 745, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 397, 833
HpyF3I CTNAG 2 cut(s) 323, 971
Hsp92II CATG 6 cut(s) 197, 227, 460, 499, 749, 947
KroI GCCGGC 1 cut(s) 836
KroNI GCCGGC 1 cut(s) 838
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
LmnI GCTCC 3 cut(s) 197, 280, 884
Lsp1109I GCAGC 2 cut(s) 622, 974
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 270, 440
MaeIII GTNAC 2 cut(s) 376, 541
MalI GATC 7 cut(s) 83, 180, 405, 683, 712, 753, 1029
MboI GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
MboII GAAGA 4 cut(s) 60, 61, 172, 398
MfeI CAATTG 1 cut(s) 211
MflI RGATCY 2 cut(s) 403, 710
MhlI GDGCHC 3 cut(s) 8, 277, 889
MluCI AATT 8 cut(s) 40, 123, 147, 211, 727, 740, 890, 948
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 2 cut(s) 273, 886
Mph1103I ATGCAT 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 836
MseI TTAA 3 cut(s) 453, 624, 804
MspI CCGG 3 cut(s) 24, 410, 837
MspR9I CCNGG 3 cut(s) 157, 167, 279
MunI CAATTG 1 cut(s) 211
MvaI CCWGG 3 cut(s) 157, 167, 279
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 397, 833
NaeI GCCGGC 1 cut(s) 838
NdeI CATATG 1 cut(s) 508
NdeII GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
NgoMIV GCCGGC 1 cut(s) 836
NlaIII CATG 6 cut(s) 197, 227, 460, 499, 749, 947
NlaIV GGNNCC 2 cut(s) 154, 870
NmeAIII GCCGAG 1 cut(s) 946
NmuCI GTSAC 2 cut(s) 376, 541
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 1 cut(s) 749
NspV TTCGAA 1 cut(s) 788
PaeI GCATGC 1 cut(s) 749
PagI TCATGA 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 838
PfeI GAWTC 3 cut(s) 383, 492, 921
PflMI CCANNNNNTGG 2 cut(s) 353, 698
PkrI GCNGC 2 cut(s) 612, 989
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
PshAI GACNNNNGTC 1 cut(s) 167
Psp124BI GAGCTC 2 cut(s) 277, 889
Psp6I CCWGG 3 cut(s) 155, 165, 277
PspGI CCWGG 3 cut(s) 155, 165, 277
PspN4I GGNNCC 2 cut(s) 154, 870
PspPI GGNCC 3 cut(s) 169, 842, 953
PsrI GAACNNNNNNTAC 2 cut(s) 670, 702
PsuI RGATCY 2 cut(s) 403, 710
RsaI GTAC 2 cut(s) 64, 504
RsaNI GTAC 2 cut(s) 63, 503
SacI GAGCTC 2 cut(s) 277, 889
SaqAI TTAA 3 cut(s) 453, 624, 804
SatI GCNGC 2 cut(s) 611, 988
Sau3AI GATC 7 cut(s) 81, 178, 403, 681, 710, 751, 1027
Sau96I GGNCC 3 cut(s) 169, 842, 953
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 3 cut(s) 157, 167, 279
SduI GDGCHC 3 cut(s) 8, 277, 889
SfaNI GCATC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 788
SinI GGWCC 3 cut(s) 169, 842, 953
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
SphI GCATGC 1 cut(s) 749
Sse9I AATT 8 cut(s) 40, 123, 147, 211, 727, 740, 890, 948
SsiI CCGC 4 cut(s) 45, 139, 840, 1004
SspMI CTAG 2 cut(s) 270, 440
SstI GAGCTC 2 cut(s) 277, 889
StyD4I CCNGG 3 cut(s) 155, 165, 277
StyI CCWWGG 2 cut(s) 76, 439
TaaI ACNGT 3 cut(s) 502, 629, 809
TaqI TCGA 3 cut(s) 237, 788, 1030
TasI AATT 8 cut(s) 40, 123, 147, 211, 727, 740, 890, 948
TatI WGTACW 1 cut(s) 62
TfiI GAWTC 3 cut(s) 383, 492, 921
Tru1I TTAA 3 cut(s) 453, 624, 804
Tru9I TTAA 3 cut(s) 453, 624, 804
TscAI CASTG 4 cut(s) 634, 814, 852, 1013
TseFI GTSAC 2 cut(s) 376, 541
TseI GCWGC 2 cut(s) 610, 987
Tsp45I GTSAC 2 cut(s) 376, 541
TspDTI ATGAA 3 cut(s) 113, 240, 480
TspRI CASTG 4 cut(s) 634, 814, 852, 1013
Van91I CCANNNNNTGG 2 cut(s) 353, 698
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 3 cut(s) 169, 842, 953
XapI RAATTY 3 cut(s) 40, 123, 727
XceI RCATGY 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 2 cut(s) 270, 440
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.