Rmu_ssc0000255.1_g000038

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000255.1
OG0006733
Physical Location & Seq
Reverse (-)
237442 .. 246294
8853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1857 bp
atggccttgtttcgcaggctgcttcctcctctctgctcctccatcaacaggacgagcttttgctcatctggagctctacaaggttttcggagttatggagccattgatacacccgagccaaccgtgttgaaatttgagaatggagagacggctgattctgtgactgctggtgatgagatagcaaagattcggcgtgaatttgatgccgcaaagcagagtttcctcaagattccacaggcacttcaagaattgccgaagatgaatccggaaggtttgtcagtccgaattcatttaaatttaggttttcggagttatggagccattgatacacccgagccaaccgtgttgaagaaatttgagaatggagagaaggctgatgctgtgactgctgatgatgagatagcaaagattcggcgtgaattcgatgccgcaaagcagagtttcctcaagattccacaggcacttcaagaattgccgaagatgaatccggaagggatatatgtaaataaaaacctcaggttggacagtattcaagtttatggatttgactatgactacacgctggtgcattactcggcagatttacagaacttgatatatgatcttgctaaggagcatatggtcaatgagttccggtatcctcatgtttgcatggaatttaagtacgatcctacttttcccattagaggactgtgctatgacaaattgaaagggtgtctcttgaagctggacttttttgggtcaattgagccagatggatgctactatggtcgtcgaaagcttagtaggaaggaaatagatgagatatatggcacaaggcgtatcggtcgtgatcaagcaggaagccttgttgggttgatggatttcttctgctttagtgaggcatgcctgattgcagacatcgtacaatattttgtggatgctaagctggaatttgatgcttgctacatctatcaagatgtaaatcgtgcgattcagcatgttcaccatacaggccttgttcatagaggaattctttctgatccccgtaaatatcttgttaaaaatgaccagctgttacactttctgaagatgctcagggaaaaaggaaagaaacttttcttgctgactaactctccatattactttgtggatagaggaatgcgttttttgttggaggattctttgggttgtaatgactcttggagggaactttttgacgttgtgattgccaaagccaataagccagatttctacacatctgagcatccattccgttgttatgatgcagagaaggataatttagctttcatgaaggtggatgcgtttcttcctaataaaatttattaccacggatgcctgaaatcattccttcaaattactaagtggaagggaccagaggtgatatactttggtgatcacctctttagcgacctaaggggaccatcaaaagctggttggcgcactgctgctataatccatgaactagaagatgaaatacgtatacaaaatgaggatgcttaccgcgtggagcaggcaaaatttcatatattccaagaattgcttggtagactgcatgcaacggtagctaatagtcagaaaagtgaggccaagttactgttggaggagctaaatgagtgcaggcgtaaggcaagctacaagatgaagagaatgttcaatagctctttcggagccactttccttacggataaaggtcaagaatctgctttctcgtatcatattcatcaatatgcagatgtctataccagtaagccagagaattttttgctctatccacctgaagcatggcttcatgcaccctacgatattaagattatgccacatcatgtgaaggttcgtctgacttatatcttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

618

Amino Acids

71.52

Weight (kDa)

7.04

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 166 - 610 1.1e-148 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1483, 1549
AccII CGCG 1 cut(s) 1506
AccIII TCCGGA 2 cut(s) 265, 487
AciI CCGC 3 cut(s) 207, 429, 1504
AclWI GGATC 2 cut(s) 662, 1016
AcuI CTGAAG 2 cut(s) 1088, 1800
AfaI GTAC 2 cut(s) 665, 906
AfiI CCNNNNNNNGG 3 cut(s) 48, 520, 686
AgsI TTSAA 9 cut(s) 130, 245, 349, 467, 533, 709, 724, 1355, 1657
AleI CACNNNNGTG 1 cut(s) 563
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 2 cut(s) 140, 722
AlwI GGATC 2 cut(s) 662, 1016
Ama87I CYCGRG 2 cut(s) 113, 332
Aor13HI TCCGGA 2 cut(s) 265, 487
AoxI GGCC 3 cut(s) 3, 994, 1587
ApeKI GCWGC 2 cut(s) 19, 1448
Asp700I GAANNNNTTC 3 cut(s) 1015, 1097, 1346
AspLEI GCGC 1 cut(s) 1443
AspS9I GGNCC 2 cut(s) 1373, 1421
AsuHPI GGTGA 5 cut(s) 182, 977, 1391, 1393, 1406
AvaI CYCGRG 2 cut(s) 113, 332
AvaII GGWCC 2 cut(s) 1373, 1421
AxyI CCTNAGG 2 cut(s) 515, 1415
BanII GRGCYC 1 cut(s) 76
BarI GAAGNNNNNNTAC 2 cut(s) 1461, 1493
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 6, 1435
BccI CCATC 4 cut(s) 50, 749, 853, 1432
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 2 cut(s) 832, 1396
BcoDI GTCTC 2 cut(s) 140, 722
BfaI CTAG 1 cut(s) 1466
BfuI GTATCC 1 cut(s) 648
BisI GCNGC 4 cut(s) 20, 207, 429, 1449
BlpI GCTNAGC 1 cut(s) 924
BlsI GCNGC 4 cut(s) 21, 208, 430, 1450
Bme18I GGWCC 2 cut(s) 1373, 1421
BmeT110I CYCGRG 2 cut(s) 113, 332
BmgT120I GGNCC 2 cut(s) 1373, 1421
BmiI GGNNCC 5 cut(s) 100, 319, 1374, 1422, 1672
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 2 cut(s) 609, 1076
Bpu1102I GCTNAGC 1 cut(s) 924
BpuEI CTTGAG 2 cut(s) 209, 431
BsaAI YACGTR 1 cut(s) 1481
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 1 cut(s) 1330
BsaWI WCCGGW 3 cut(s) 265, 487, 633
BsaXI ACNNNNNCTCC 2 cut(s) 1099, 1129
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 520, 686
Bse1I ACTGG 1 cut(s) 1746
Bse21I CCTNAGG 2 cut(s) 515, 1415
Bse8I GATNNNNATC 1 cut(s) 963
BseAI TCCGGA 2 cut(s) 265, 487
BseDI CCNNGG 1 cut(s) 1330
BseGI GGATG 6 cut(s) 764, 925, 1246, 1306, 1340, 1501
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 3 cut(s) 48, 520, 686
BseMII CTCAG 3 cut(s) 529, 1090, 1233
BseNI ACTGG 1 cut(s) 1746
BseRI GAGGAG 3 cut(s) 18, 28, 1619
BseXI GCAGC 2 cut(s) 6, 1435
BsgI GTGCAG 1 cut(s) 1639
Bsh1236I CGCG 1 cut(s) 1506
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 3 cut(s) 5, 996, 1589
BsiEI CGRYCG 1 cut(s) 829
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 2 cut(s) 113, 332
BsiSI CCGG 3 cut(s) 266, 488, 634
BslFI GGGAC 2 cut(s) 1386, 1434
BslI CCNNNNNNNGG 3 cut(s) 48, 520, 686
BsmAI GTCTC 2 cut(s) 140, 722
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 1386, 1434
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 3 cut(s) 5, 996, 1589
BsoBI CYCGRG 2 cut(s) 113, 332
Bsp1286I GDGCHC 1 cut(s) 76
Bsp13I TCCGGA 2 cut(s) 265, 487
Bsp143I GATC 5 cut(s) 601, 667, 832, 1021, 1396
Bsp1720I GCTNAGC 1 cut(s) 924
BspACI CCGC 3 cut(s) 207, 429, 1504
BspANI GGCC 3 cut(s) 5, 996, 1589
BspCNI CTCAG 3 cut(s) 528, 1089, 1234
BspEI TCCGGA 2 cut(s) 265, 487
BspFNI CGCG 1 cut(s) 1506
BspHI TCATGA 1 cut(s) 1290
BspLI GGNNCC 5 cut(s) 100, 319, 1374, 1422, 1672
BspPI GGATC 2 cut(s) 662, 1016
BsrI ACTGG 1 cut(s) 1746
BssECI CCNNGG 1 cut(s) 1330
BssMI GATC 5 cut(s) 601, 667, 832, 1021, 1396
BssNAI GTATAC 1 cut(s) 1484
Bst1107I GTATAC 1 cut(s) 1484
Bst4CI ACNGT 6 cut(s) 124, 343, 527, 693, 1564, 1599
Bst6I CTCTTC 1 cut(s) 1640
BstBAI YACGTR 1 cut(s) 1481
BstC8I GCNNGC 7 cut(s) 17, 886, 943, 1515, 1557, 1622, 1633
BstDEI CTNAG 8 cut(s) 515, 609, 782, 924, 1076, 1242, 1362, 1415
BstDSI CCRYGG 1 cut(s) 1330
BstF5I GGATG 6 cut(s) 764, 925, 1246, 1306, 1340, 1501
BstFNI CGCG 1 cut(s) 1506
BstHHI GCGC 1 cut(s) 1443
BstKTI GATC 5 cut(s) 604, 670, 835, 1024, 1399
BstMAI GTCTC 2 cut(s) 140, 722
BstMBI GATC 5 cut(s) 601, 667, 832, 1021, 1396
BstMCI CGRYCG 1 cut(s) 829
BstMWI GCNNNNNNNGC 2 cut(s) 386, 1565
BstNSI RCATGY 3 cut(s) 888, 983, 1559
BstSNI TACGTA 1 cut(s) 1481
BstUI CGCG 1 cut(s) 1506
BstV1I GCAGC 2 cut(s) 6, 1435
BstZ17I GTATAC 1 cut(s) 1484
Bsu36I CCTNAGG 2 cut(s) 515, 1415
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 3 cut(s) 5, 996, 1589
BtgI CCRYGG 1 cut(s) 1330
BtsCI GGATG 6 cut(s) 764, 925, 1246, 1306, 1340, 1501
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 1 cut(s) 1443
Cac8I GCNNGC 7 cut(s) 17, 886, 943, 1515, 1557, 1622, 1633
CciI TCATGA 1 cut(s) 1290
CfoI GCGC 1 cut(s) 1443
Cfr13I GGNCC 2 cut(s) 1373, 1421
Csp6I GTAC 2 cut(s) 664, 905
CviQI GTAC 2 cut(s) 664, 905
DdeI CTNAG 8 cut(s) 515, 609, 782, 924, 1076, 1242, 1362, 1415
DpnI GATC 5 cut(s) 603, 669, 834, 1023, 1398
DpnII GATC 5 cut(s) 601, 667, 832, 1021, 1396
DraI TTTAAA 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 1640
EarI CTCTTC 1 cut(s) 1640
Ecl136II GAGCTC 1 cut(s) 74
Eco105I TACGTA 1 cut(s) 1481
Eco147I AGGCCT 1 cut(s) 996
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 1373, 1421
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 2 cut(s) 1088, 1800
Eco81I CCTNAGG 2 cut(s) 515, 1415
Eco88I CYCGRG 2 cut(s) 113, 332
EcoICRI GAGCTC 1 cut(s) 74
EcoRI GAATTC 3 cut(s) 285, 419, 1011
EcoT38I GRGCYC 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 140
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 1527, 1559, 1773, 1805
FaqI GGGAC 2 cut(s) 1386, 1434
FauNDI CATATG 1 cut(s) 618
FbaI TGATCA 2 cut(s) 832, 1396
FblI GTMKAC 2 cut(s) 1483, 1549
Fnu4HI GCNGC 4 cut(s) 20, 207, 429, 1449
FokI GGATG 6 cut(s) 771, 932, 1233, 1313, 1347, 1508
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 4 cut(s) 20, 207, 429, 1449
FspBI CTAG 1 cut(s) 1466
GlaI GCGC 1 cut(s) 1442
GluI GCNGC 4 cut(s) 20, 207, 429, 1449
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 3 cut(s) 5, 996, 1589
HapII CCGG 3 cut(s) 266, 488, 634
HhaI GCGC 1 cut(s) 1443
Hin6I GCGC 1 cut(s) 1441
HinP1I GCGC 1 cut(s) 1441
HindIII AAGCTT 1 cut(s) 779
HpaII CCGG 3 cut(s) 266, 488, 634
HphI GGTGA 5 cut(s) 182, 977, 1391, 1393, 1406
Hpy166II GTNNAC 3 cut(s) 985, 1484, 1550
Hpy188I TCNGA 9 cut(s) 90, 284, 309, 1021, 1068, 1243, 1578, 1670, 1842
Hpy8I GTNNAC 3 cut(s) 985, 1484, 1550
Hpy99I CGWCG 1 cut(s) 777
HpyAV CCTTC 9 cut(s) 263, 364, 485, 784, 1267, 1288, 1361, 1363, 1825
HpyCH4III ACNGT 6 cut(s) 124, 343, 527, 693, 1564, 1599
HpyCH4IV ACGT 2 cut(s) 1200, 1480
HpyCH4V TGCA 9 cut(s) 568, 651, 896, 1268, 1555, 1559, 1620, 1733, 1796
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 1565
HpyF3I CTNAG 8 cut(s) 515, 609, 782, 924, 1076, 1242, 1362, 1415
HpySE526I ACGT 2 cut(s) 1200, 1480
HspAI GCGC 1 cut(s) 1441
Kpn2I TCCGGA 2 cut(s) 265, 487
Ksp22I TGATCA 2 cut(s) 832, 1396
Kzo9I GATC 5 cut(s) 601, 667, 832, 1021, 1396
LmnI GCTCC 8 cut(s) 41, 71, 98, 317, 613, 1510, 1606, 1670
Lsp1109I GCAGC 2 cut(s) 6, 1435
MaeI CTAG 1 cut(s) 1466
MaeII ACGT 2 cut(s) 1200, 1480
MaeIII GTNAC 4 cut(s) 160, 382, 1056, 1593
MalI GATC 5 cut(s) 603, 669, 834, 1023, 1398
MboI GATC 5 cut(s) 601, 667, 832, 1021, 1396
MboII GAAGA 8 cut(s) 268, 361, 490, 859, 1081, 1301, 1481, 1657
MfeI CAATTG 1 cut(s) 744
MhlI GDGCHC 1 cut(s) 76
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 3 cut(s) 501, 1134, 1581
MroI TCCGGA 2 cut(s) 265, 487
MroXI GAANNNNTTC 3 cut(s) 1015, 1097, 1346
MseI TTAA 5 cut(s) 293, 660, 1041, 1809, 1855
MslI CAYNNNNRTG 3 cut(s) 563, 1295, 1728
MspA1I CMGCKG 1 cut(s) 1054
MspI CCGG 3 cut(s) 266, 488, 634
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 1146
MvnI CGCG 1 cut(s) 1506
MwoI GCNNNNNNNGC 2 cut(s) 386, 1565
NdeI CATATG 1 cut(s) 618
NdeII GATC 5 cut(s) 601, 667, 832, 1021, 1396
NlaIV GGNNCC 5 cut(s) 100, 319, 1374, 1422, 1672
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 2 cut(s) 160, 382
NspI RCATGY 3 cut(s) 888, 983, 1559
OliI CACNNNNGTG 1 cut(s) 563
PaeI GCATGC 2 cut(s) 888, 1559
PagI TCATGA 1 cut(s) 1290
PceI AGGCCT 1 cut(s) 996
PctI GAATGC 1 cut(s) 1146
PdmI GAANNNNTTC 3 cut(s) 1015, 1097, 1346
PkrI GCNGC 4 cut(s) 21, 208, 430, 1450
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
Ppu21I YACGTR 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 76
PspN4I GGNNCC 5 cut(s) 100, 319, 1374, 1422, 1672
PspPI GGNCC 2 cut(s) 1373, 1421
PvuII CAGCTG 1 cut(s) 1054
RsaI GTAC 2 cut(s) 665, 906
RsaNI GTAC 2 cut(s) 664, 905
RseI CAYNNNNRTG 3 cut(s) 563, 1295, 1728
SacI GAGCTC 1 cut(s) 76
SaqAI TTAA 5 cut(s) 293, 660, 1041, 1809, 1855
SatI GCNGC 4 cut(s) 20, 207, 429, 1449
Sau3AI GATC 5 cut(s) 601, 667, 832, 1021, 1396
Sau96I GGNCC 2 cut(s) 1373, 1421
SchI GAGTC 1 cut(s) 1172
SduI GDGCHC 1 cut(s) 76
SinI GGWCC 2 cut(s) 1373, 1421
SmiI ATTTAAAT 1 cut(s) 294
SmiMI CAYNNNNRTG 3 cut(s) 563, 1295, 1728
SmlI CTYRAG 2 cut(s) 224, 446
SmoI CTYRAG 2 cut(s) 224, 446
SnaBI TACGTA 1 cut(s) 1481
SphI GCATGC 2 cut(s) 888, 1559
SseBI AGGCCT 1 cut(s) 996
SsiI CCGC 3 cut(s) 207, 429, 1504
SspI AATATT 1 cut(s) 911
SspMI CTAG 1 cut(s) 1466
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 996
SwaI ATTTAAAT 1 cut(s) 294
TaaI ACNGT 6 cut(s) 124, 343, 527, 693, 1564, 1599
TaiI ACGT 2 cut(s) 1203, 1483
TaqI TCGA 2 cut(s) 423, 775
TaqII GACCGA 1 cut(s) 815
TauI GCSGC 2 cut(s) 209, 431
Tru1I TTAA 5 cut(s) 293, 660, 1041, 1809, 1855
Tru9I TTAA 5 cut(s) 293, 660, 1041, 1809, 1855
TscAI CASTG 1 cut(s) 1450
TseFI GTSAC 2 cut(s) 160, 382
TseI GCWGC 2 cut(s) 19, 1448
Tsp45I GTSAC 2 cut(s) 160, 382
TspGWI ACGGA 3 cut(s) 1244, 1347, 1700
TspRI CASTG 1 cut(s) 1450
VpaK11BI GGWCC 2 cut(s) 1373, 1421
XceI RCATGY 3 cut(s) 888, 983, 1559
XcmI CCANNNNNNNNNTGG 3 cut(s) 1541, 1597, 1782
XmiI GTMKAC 2 cut(s) 1483, 1549
XmnI GAANNNNTTC 3 cut(s) 1015, 1097, 1346
XspI CTAG 1 cut(s) 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.