Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000387.1

Orthogroup ID

OG0004888

Physical Location & Seq

Genomic Coordinates Reverse (-)

10129 .. 17781

Gene Structure 7653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3870 bp

atgaattcctttgtgctttttcagtcctctttgattcctctgcttttgatcacttcctttctgtcgatcaattttcaattgagtgtgtgcgccgatgatgcatatgctaactgcagccaacctattacctgcgggagtgtcaagagcaacatctcttaccccttttggggagcgaaccgagccgattactgtggcaaatctgggttcgaggtcacatgccaagacgatgtcccgataatcagcatgcagaaaatcaatttcagaattattgacatgaacaatagcactactacaccaactccgactgtgacagttgcaaggcaggattattgggaaactctctgtcctgagagttatattgacacaaacctcaacttctctctgtttgattacgcatccgggcctcaaaacgtgtcgttttactacggatgcagtccgactgcaacttcagcgatgtcagcgataggatttaatttttctagtatctcacacaaatgcaacgataccgtcacagtttcctttctcacagagaagcaaattgctaaccttcccgatacaactgccttgcgctcctgtcaacctgtggttttggttccggtgtctgcggcagctgctgaagcgcttgacaacaatcaaacagcaactattcagaatgcggtagatggtggttttgaattgaatgtgcagaatgatgatactgctttttgcaacaattgcgtggcctcaggaggagtttgtgggcaagacactactaatgccaagttcatttgctattgccagaatttaaattcaacaggaacatgtcttagatctaaccaaaccacacagtctctatctccatctctagaaatgcatccctatcttctcctcctgcagctcttaataataatactagtattcttatctggggtaccaaaatccttgggggctgatgatgagcggtacctcaactgcagccaatcgtttgattgtgcaagtatcaggagcatcggttaccctttctggggatcaagccgacccgactactgtggcttcccggagttcaagttgaactgcagcggcgacgccctgtccatctcaatcaaggaacaagaatttcgagtcctttctatcaatcagagtcaaagtagtttcacaattgctagagccgattactggaacaccgtttgtccggtatcccttaccaataccaccaccatagataattcatcatcccctgttcttgaatttgcttcggatgttcaacaactctcgatcttttattcatgccctccacttaatattcctctcccacctcagcttaccagccaattcaactgcaccataaactctacggattccatcaactactatgtgacgaaagatcttgacattttcgggctctccaacatcagtagcacctttggaacgtgtcgcacaactgtcaccgtccgagtttctcaatcggcagcttccaatttagcattgaatacgtccaaggaaaatctaggcgcggcgcttgattccggttttaatttgatatgggatgcgagcaataccttgtgcaaacaatgtcaagactctggaggtcaatgcggctacaatacatcttcagctgaattcacctgttattgcaaggatggacctaatccctctgattgtagagctgcgatatactcacaagtcacaacagtccaagtcaaacttgtatggccgccccctcagctaaccacaccccaaaaccttcaccaagtccctaactgtgcaacccctctctccctctctctctctctctctctctcaactttccaatgccttcaatttttctctctccgtctccaatcttcacccttttccttcttcttcgtcttcttatgctccgtcaacttcaccctttctctttcgcccaactcaagcctccctaactgcacaaacaccttcaactgtggcacactccacaacctgacttaccccttcaccggcggtgaccgcccgtcctactgcggcccgccggagttccatatcaactgcttaaacgactcgccggagctgtccatcatgtcgctgacttaccgagtccttgctctcgaccaagctgttcaaacgctgagcttagcccgcacggacctctggaacgagaactgcacaggtgacttggctaactcaagtatttcttccggtttcttcagcttcagcgacgccgataacgtcgacatcaccattttctacggttgcaattcttctagtattctgcccaagcccgagaattggttccactgtaatatcaatcagacttctaacgatgcgtactacttgatcggtccggttcctctcgatccaattatgagcacatttaagtgttttattggcatcacggtgccgattctcaaactcactgcggctaagctcgttacgaatcggtctctgtttaagcaagcgattatgggtggcttcaacgctagctatactaatccctacctccgtcagtgcaataagtgtcttaatgtaaatggggaatgtgggtttgactccgattcgaaccggcccgtttgcatttgtggcggtcgcctgtgtgacccagcaggaaagaataatcaaataccaataggtcttgctgttggaggtgcaatcctttttggcattttcgtgggattctacgcatattttgtcatccagcgaaagaagaagaaacgtgcagcagaagctcagagcaaagagattcccaccccacttacaagcaaaagcactgttactccctcgactaatctttctcaatctcaaagcatcccttcttatccttcctttaactcaaaatcaaggtctgaccttggaaaagccagcacttactttggagttcaggtcttcagctatgctgaattagaggaagccactgaaaatttcaaccctgctaaagaacttggagatggaggctttggcaccgtttactatggtaagctacatgatggccgtgtagttgcagtgaagcgactttatgaaaacaatttcaaacgggtggagcagtttatgaatgaggttgaaatcttaacacgtctggaacaccagcatcttgtgaaactctatggatgcacctcaagacgtagccgagaactcctccttgtctatgaatatatttctaatggaacagtggctgaccatctccatggaaaacgaatggagtctggctttctcagttggcctgttagattgagcattgccatagagacagctgatgcactggctttccttcatcgcaatgatgtgatacaccgtgatgtcaaaaccaacaatatacttctggatgacgacttttgtgtgaaagttgctgattttggtctgtcacggttgttccccaatgatcagacccatgtgtcaactgctccacaagggacgccgggctatgttgatcccgagtattaccagtgctatcaactgactgacaaaagtgatgtttatagctttggggtggtcttgatcgagcttatatcatccttacaagctgtcgacaccaacaggcaccggcatgatataaatttggccaacatggctattaacaaaattcaaaatcatttggtgaatgagttggttgatccacttcttgggtatgacaaggactattctgtgaggataatggcaacagctgttgcagaattggccttccggtgtttgcagcaggagaaagatatgagaccaaccatggaagaagtactggagggtttaaaagcaattcaaaatgaagaccttggtgcacaagaaaatggggaggcagaggcagtggtcctggatataggagctgatgaagttgggcttttgaggaatttgcctcccccactctcaccagactcggttgtcactgataaattgattttaaaaaggtgggacagaactatgtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

1289

Amino Acids

141.86

Weight (kDa)

5.03

Isoelectric Point (pI)

41.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	38 - 98	2.8e-12	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	172 - 261	4.4e-15	Wall-associated receptor kinase C-terminal
GUB_WAK_bind	PF13947	318 - 382	3.9e-14	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	440 - 541	1.1e-19	Wall-associated receptor kinase C-terminal
GUB_WAK_bind	PF13947	636 - 700	3.7e-12	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	775 - 848	2.5e-10	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	962 - 1230	4.1e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	963 - 1231	3.6e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004888)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66880 AT1G66880 AT1G66880 AT5G38210 AT5G38210 AT5G38210
fragaria_vesca	FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540
malus_domestica	MD00G1204100.v1.1 MD09G1255300.v1.1 MD17G1247800.v1.1 MD17G1248000.v1.1
prunus_persica	Prupe.3G146500_v2.0.a1 Prupe.3G146700_v2.0.a1
pyrus_communis	pycom09g17110 pycom17g24910 pycom17g25040
rosa_chinensis	RchiOBHm_Chr6g0273041
rosa_laevigata	RLG00000013645
rosa_multiflora	Rmu_ssc0000387.1_g000003
rosa_roxburghii	Rroxscaffold_7G00195700
rosa_rugosa	Rorug06G0072500
rosa_samantha	Rh6AG188100 Rh6BG191500 Rh6CG190600 Rh6DG182000
rosa_wichuraiana	Rw6G016310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	3346, 3824
Acc36I	ACCTGC	1 cut(s)	137
Acc65I	GGTACC	2 cut(s)	910, 942
AccB1I	GGYRCC	5 cut(s)	910, 942, 2356, 2915, 3492
AccB7I	CCANNNNNTGG	1 cut(s)	3575
AccBSI	CCGCTC	1 cut(s)	940
AccI	GTMKAC	2 cut(s)	2190, 3480
AccII	CGCG	1 cut(s)	1493
AclWI	GGATC	4 cut(s)	1015, 2309, 3377, 3560
AcoI	YGGCCR	3 cut(s)	1691, 2944, 3513
AcuI	CTGAAG	6 cut(s)	432, 636, 1575, 2149, 2155, 2827
AcyI	GRCGYC	3 cut(s)	1065, 2178, 3368
AfaI	GTAC	4 cut(s)	912, 944, 2288, 3684
AfeI	AGCGCT	1 cut(s)	621
AfiI	CCNNNNNNNGG	8 cut(s)	166, 167, 1003, 1004, 1155, 1958, 2247, 3575
AflIII	ACRYGT	4 cut(s)	411, 800, 1409, 3026
AhdI	GACNNNNNGTC	2 cut(s)	1069, 1972
AhlI	ACTAGT	1 cut(s)	892
AjiI	CACGTC	1 cut(s)	3029
AjnI	CCWGG	1 cut(s)	3756
Alw21I	GWGCWC	2 cut(s)	2330, 3727
Alw26I	GTCTC	5 cut(s)	834, 1820, 2406, 3194, 3658
Alw44I	GTGCAC	1 cut(s)	3723
AlwI	GGATC	4 cut(s)	1015, 2309, 3377, 3560
AlwNI	CAGNNNCTG	2 cut(s)	614, 3128
Ama87I	CYCGRG	2 cut(s)	2240, 3386
Aor51HI	AGCGCT	1 cut(s)	621
AoxI	GGCC	9 cut(s)	401, 720, 1691, 1984, 2522, 2944, 3173, 3513, 3630
ApaLI	GTGCAC	1 cut(s)	3723
ArsI	GACNNNNNNTTYG	2 cut(s)	2783, 2815
Asp700I	GAANNNNTTC	1 cut(s)	2249
Asp718I	GGTACC	2 cut(s)	910, 942
AspLEI	GCGC	5 cut(s)	92, 570, 622, 1493, 1498
AspS9I	GGNCC	7 cut(s)	401, 1622, 1985, 2104, 2300, 2523, 3754
AsuC2I	CCSGG	3 cut(s)	400, 1037, 3372
AsuII	TTCGAA	1 cut(s)	2516
AsuNHI	GCTAGC	1 cut(s)	2438
AvaI	CYCGRG	2 cut(s)	2240, 3386
AvaII	GGWCC	4 cut(s)	1622, 2104, 2300, 3754
AxyI	CCTNAGG	1 cut(s)	724
BaeGI	GKGCMC	1 cut(s)	3727
BalI	TGGCCA	1 cut(s)	3515
BanI	GGYRCC	5 cut(s)	910, 942, 2356, 2915, 3492
BanII	GRGCYC	1 cut(s)	1383
BbsI	GAAGAC	3 cut(s)	1840, 2833, 3720
Bbv12I	GWGCWC	2 cut(s)	2330, 3727
BbvCI	CCTCAGC	2 cut(s)	1296, 1701
BccI	CCATC	9 cut(s)	656, 847, 1082, 1349, 1613, 2042, 2897, 2936, 3141
BceAI	ACGGC	1 cut(s)	2931
BcgI	CGANNNNNNTGC	2 cut(s)	542, 576
BciT130I	CCWGG	1 cut(s)	3758
BciVI	GTATCC	1 cut(s)	1186
BclI	TGATCA	2 cut(s)	48, 3334
BcnI	CCSGG	3 cut(s)	400, 1037, 3372
BcoDI	GTCTC	5 cut(s)	834, 1820, 2406, 3194, 3658
BcuI	ACTAGT	1 cut(s)	892
BfaI	CTAG	7 cut(s)	480, 845, 893, 1143, 1487, 2223, 2439
BfmI	CTRYAG	4 cut(s)	112, 872, 952, 1054
BfoI	RGCGCY	2 cut(s)	623, 1499
BfuAI	ACCTGC	1 cut(s)	137
BfuI	GTATCC	1 cut(s)	1186
BglI	GCCNNNNNGGC	1 cut(s)	3521
BglII	AGATCT	2 cut(s)	809, 1363
BlpI	GCTNAGC	3 cut(s)	2087, 2092, 2382
BmcAI	AGTACT	1 cut(s)	3684
Bme1390I	CCNGG	4 cut(s)	400, 1037, 3372, 3758
Bme18I	GGWCC	4 cut(s)	1622, 2104, 2300, 3754
BmeRI	GACNNNNNGTC	2 cut(s)	1069, 1972
BmeT110I	CYCGRG	2 cut(s)	2240, 3386
BmgBI	CACGTC	1 cut(s)	3029
BmgT120I	GGNCC	7 cut(s)	401, 1622, 1985, 2104, 2300, 2523, 3754
BmiI	GGNNCC	8 cut(s)	594, 912, 944, 2252, 2307, 2358, 2917, 3494
BmrFI	CCNGG	4 cut(s)	400, 1037, 3372, 3758
BmtI	GCTAGC	1 cut(s)	2442
BpiI	GAAGAC	3 cut(s)	1840, 2833, 3720
BplI	GAGNNNNNCTC	2 cut(s)	3075, 3107
BpmI	CTGGAG	2 cut(s)	1584, 3707
Bpu10I	CCTNAGC	2 cut(s)	1296, 1701
Bpu1102I	GCTNAGC	3 cut(s)	2087, 2092, 2382
Bpu14I	TTCGAA	1 cut(s)	2516
BpuEI	CTTGAG	3 cut(s)	1876, 2128, 3055
BpuMI	CCSGG	3 cut(s)	400, 1037, 3372
BsaHI	GRCGYC	3 cut(s)	1065, 2178, 3368
BsaI	GGTCTC	2 cut(s)	2406, 3658
BsaJI	CCNNGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
BsaWI	WCCGGW	6 cut(s)	595, 1171, 1505, 2156, 2302, 3636
Bsc4I	CCNNNNNNNGG	8 cut(s)	166, 167, 1003, 1004, 1155, 1958, 2247, 3575
Bse118I	RCCGGY	3 cut(s)	1958, 2520, 3495
Bse1I	ACTGG	4 cut(s)	1160, 3219, 3397, 3690
Bse21I	CCTNAGG	1 cut(s)	724
Bse3DI	GCAATG	2 cut(s)	3189, 3238
BseBI	CCWGG	1 cut(s)	3758
BseDI	CCNNGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
BseLI	CCNNNNNNNGG	8 cut(s)	166, 167, 1003, 1004, 1155, 1958, 2247, 3575
BseMI	GCAATG	2 cut(s)	3189, 3238
BseMII	CTCAG	7 cut(s)	339, 738, 1310, 1715, 2078, 2699, 3181
BseNI	ACTGG	4 cut(s)	1160, 3219, 3397, 3690
BseRI	GAGGAG	3 cut(s)	744, 857, 3080
BseSI	GKGCMC	1 cut(s)	3727
BseYI	CCCAGC	1 cut(s)	2557
BsgI	GTGCAG	5 cut(s)	704, 1303, 1891, 2107, 2694
Bsh1236I	CGCG	1 cut(s)	1493
Bsh1285I	CGRYCG	1 cut(s)	2545
BshFI	GGCC	9 cut(s)	403, 722, 1693, 1986, 2524, 2946, 3175, 3515, 3632
BshNI	GGYRCC	5 cut(s)	910, 942, 2356, 2915, 3492
BsiEI	CGRYCG	1 cut(s)	2545
BsiHKAI	GWGCWC	2 cut(s)	2330, 3727
BsiHKCI	CYCGRG	2 cut(s)	2240, 3386
BslFI	GGGAC	3 cut(s)	215, 1718, 3379
BslI	CCNNNNNNNGG	8 cut(s)	166, 167, 1003, 1004, 1155, 1958, 2247, 3575
BsmAI	GTCTC	5 cut(s)	834, 1820, 2406, 3194, 3658
BsmBI	CGTCTC	1 cut(s)	1820
BsmFI	GGGAC	3 cut(s)	215, 1718, 3379
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	9 cut(s)	403, 722, 1693, 1986, 2524, 2946, 3175, 3515, 3632
Bso31I	GGTCTC	2 cut(s)	2406, 3658
BsoBI	CYCGRG	2 cut(s)	2240, 3386
Bsp119I	TTCGAA	1 cut(s)	2516
Bsp1286I	GDGCHC	3 cut(s)	1383, 2330, 3727
Bsp1720I	GCTNAGC	3 cut(s)	2087, 2092, 2382
Bsp19I	CCATGG	2 cut(s)	3139, 3672
BspANI	GGCC	9 cut(s)	403, 722, 1693, 1986, 2524, 2946, 3175, 3515, 3632
BspCNI	CTCAG	7 cut(s)	340, 737, 1309, 1714, 2079, 2698, 3180
BspFNI	CGCG	1 cut(s)	1493
BspLI	GGNNCC	8 cut(s)	594, 912, 944, 2252, 2307, 2358, 2917, 3494
BspMAI	CTGCAG	4 cut(s)	116, 876, 956, 1058
BspMI	ACCTGC	1 cut(s)	137
BspOI	GCTAGC	1 cut(s)	2442
BspPI	GGATC	4 cut(s)	1015, 2309, 3377, 3560
BspT104I	TTCGAA	1 cut(s)	2516
BspT107I	GGYRCC	5 cut(s)	910, 942, 2356, 2915, 3492
BspTNI	GGTCTC	2 cut(s)	2406, 3658
BsrBI	CCGCTC	1 cut(s)	940
BsrDI	GCAATG	2 cut(s)	3189, 3238
BsrFI	RCCGGY	3 cut(s)	1958, 2520, 3495
BsrI	ACTGG	4 cut(s)	1160, 3219, 3397, 3690
BssAI	RCCGGY	3 cut(s)	1958, 2520, 3495
BssECI	CCNNGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
BssNI	GRCGYC	3 cut(s)	1065, 2178, 3368
BssT1I	CCWWGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
Bst2UI	CCWGG	1 cut(s)	3758
BstACI	GRCGYC	3 cut(s)	1065, 2178, 3368
BstAPI	GCANNNNNTGC	1 cut(s)	714
BstBI	TTCGAA	1 cut(s)	2516
BstC8I	GCNNGC	7 cut(s)	245, 1531, 1988, 2098, 2415, 2440, 2818
BstDSI	CCRYGG	2 cut(s)	3139, 3672
BstEII	GGTNACC	2 cut(s)	992, 1964
BstFNI	CGCG	1 cut(s)	1493
BstH2I	RGCGCY	2 cut(s)	623, 1499
BstHHI	GCGC	5 cut(s)	92, 570, 622, 1493, 1498
BstMAI	GTCTC	5 cut(s)	834, 1820, 2406, 3194, 3658
BstMCI	CGRYCG	1 cut(s)	2545
BstNI	CCWGG	1 cut(s)	3758
BstNSI	RCATGY	3 cut(s)	219, 247, 804
BstPI	GGTNACC	2 cut(s)	992, 1964
BstSCI	CCNGG	4 cut(s)	398, 1035, 3370, 3756
BstSFI	CTRYAG	4 cut(s)	112, 872, 952, 1054
BstSLI	GKGCMC	1 cut(s)	3727
BstUI	CGCG	1 cut(s)	1493
BstV2I	GAAGAC	3 cut(s)	1840, 2833, 3720
BstX2I	RGATCY	2 cut(s)	809, 1363
BstXI	CCANNNNNNTGG	1 cut(s)	3140
BstYI	RGATCY	2 cut(s)	809, 1363
Bsu36I	CCTNAGG	1 cut(s)	724
BsuI	GTATCC	1 cut(s)	1186
BsuRI	GGCC	9 cut(s)	403, 722, 1693, 1986, 2524, 2946, 3175, 3515, 3632
BtgI	CCRYGG	2 cut(s)	3139, 3672
BtgZI	GCGATG	2 cut(s)	467, 3212
BtrI	CACGTC	1 cut(s)	3029
BtsI	GCAGTG	3 cut(s)	2373, 2964, 3756
BveI	ACCTGC	1 cut(s)	137
Cac8I	GCNNGC	7 cut(s)	245, 1531, 1988, 2098, 2415, 2440, 2818
CaiI	CAGNNNCTG	2 cut(s)	614, 3128
CfoI	GCGC	5 cut(s)	92, 570, 622, 1493, 1498
Cfr10I	RCCGGY	3 cut(s)	1958, 2520, 3495
Cfr13I	GGNCC	7 cut(s)	401, 1622, 1985, 2104, 2300, 2523, 3754
CpoI	CGGWCCG	1 cut(s)	2300
CseI	GACGC	3 cut(s)	1073, 2186, 3376
Csp6I	GTAC	4 cut(s)	911, 943, 2287, 3683
CspI	CGGWCCG	1 cut(s)	2300
CviQI	GTAC	4 cut(s)	911, 943, 2287, 3683
DraI	TTTAAA	3 cut(s)	786, 3696, 3846
DrdI	GACNNNNNNGTC	2 cut(s)	3346, 3824
DriI	GACNNNNNGTC	2 cut(s)	1069, 1972
DseDI	GACNNNNNNGTC	2 cut(s)	3346, 3824
EaeI	YGGCCR	3 cut(s)	1691, 2944, 3513
Eam1105I	GACNNNNNGTC	2 cut(s)	1069, 1972
Eco130I	CCWWGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
Eco24I	GRGCYC	1 cut(s)	1383
Eco31I	GGTCTC	2 cut(s)	2406, 3658
Eco47I	GGWCC	4 cut(s)	1622, 2104, 2300, 3754
Eco47III	AGCGCT	1 cut(s)	621
Eco57I	CTGAAG	6 cut(s)	432, 636, 1575, 2149, 2155, 2827
Eco81I	CCTNAGG	1 cut(s)	724
Eco88I	CYCGRG	2 cut(s)	2240, 3386
Eco91I	GGTNACC	2 cut(s)	992, 1964
EcoO65I	GGTNACC	2 cut(s)	992, 1964
EcoRI	GAATTC	2 cut(s)	4, 1598
EcoRII	CCWGG	1 cut(s)	3756
EcoT14I	CCWWGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
EcoT22I	ATGCAT	2 cut(s)	103, 855
EcoT38I	GRGCYC	1 cut(s)	1383
ErhI	CCWWGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
Esp3I	CGTCTC	1 cut(s)	1820
FalI	AAGNNNNNCTT	8 cut(s)	1668, 1700, 2137, 2169, 2752, 2784, 3768, 3800
FaqI	GGGAC	3 cut(s)	215, 1718, 3379
FauI	CCCGC	3 cut(s)	125, 1995, 2105
FauNDI	CATATG	1 cut(s)	103
FbaI	TGATCA	2 cut(s)	48, 3334
FblI	GTMKAC	2 cut(s)	2190, 3480
FriOI	GRGCYC	1 cut(s)	1383
FspBI	CTAG	7 cut(s)	480, 845, 893, 1143, 1487, 2223, 2439
GlaI	GCGC	5 cut(s)	91, 569, 621, 1492, 1497
GsaI	CCCAGC	1 cut(s)	2561
GsuI	CTGGAG	2 cut(s)	1584, 3707
HaeII	RGCGCY	2 cut(s)	623, 1499
HaeIII	GGCC	9 cut(s)	403, 722, 1693, 1986, 2524, 2946, 3175, 3515, 3632
HgaI	GACGC	3 cut(s)	1073, 2186, 3376
HhaI	GCGC	5 cut(s)	92, 570, 622, 1493, 1498
Hin1I	GRCGYC	3 cut(s)	1065, 2178, 3368
Hin6I	GCGC	5 cut(s)	90, 568, 620, 1491, 1496
HinP1I	GCGC	5 cut(s)	90, 568, 620, 1491, 1496
HincII	GTYRAC	5 cut(s)	578, 1864, 2191, 3351, 3481
HindII	GTYRAC	5 cut(s)	578, 1864, 2191, 3351, 3481
Hpy166II	GTNNAC	7 cut(s)	578, 1864, 2191, 2923, 3351, 3481, 3725
Hpy8I	GTNNAC	7 cut(s)	578, 1864, 2191, 2923, 3351, 3481, 3725
Hpy99I	CGWCG	3 cut(s)	1067, 2180, 2192
HpyCH4IV	ACGT	7 cut(s)	411, 1409, 1472, 2187, 2671, 3028, 3076
HpySE526I	ACGT	7 cut(s)	411, 1409, 1472, 2187, 2671, 3028, 3076
Hsp92I	GRCGYC	3 cut(s)	1065, 2178, 3368
HspAI	GCGC	5 cut(s)	90, 568, 620, 1491, 1496
KpnI	GGTACC	2 cut(s)	914, 946
Ksp22I	TGATCA	2 cut(s)	48, 3334
LmnI	GCTCC	8 cut(s)	170, 575, 984, 1862, 2026, 2995, 3361, 3767
MaeI	CTAG	7 cut(s)	480, 845, 893, 1143, 1487, 2223, 2439
MaeII	ACGT	7 cut(s)	411, 1409, 1472, 2187, 2671, 3028, 3076
MbiI	CCGCTC	1 cut(s)	940
MfeI	CAATTG	3 cut(s)	77, 712, 1137
MflI	RGATCY	2 cut(s)	809, 1363
MhlI	GDGCHC	3 cut(s)	1383, 2330, 3727
MlsI	TGGCCA	1 cut(s)	3515
MluNI	TGGCCA	1 cut(s)	3515
MlyI	GAGTC	8 cut(s)	1110, 1129, 1553, 2012, 2064, 2501, 3164, 3812
MmeI	TCCRAC	4 cut(s)	326, 461, 1410, 2578
Mox20I	TGGCCA	1 cut(s)	3515
Mph1103I	ATGCAT	2 cut(s)	103, 855
MroXI	GAANNNNTTC	1 cut(s)	2249
MscI	TGGCCA	1 cut(s)	3515
MslI	CAYNNNNRTG	8 cut(s)	493, 2335, 2354, 2624, 3138, 3231, 3249, 3498
Msp20I	TGGCCA	1 cut(s)	3515
MspA1I	CMGCKG	5 cut(s)	611, 1059, 1595, 3206, 3617
MspR9I	CCNGG	4 cut(s)	400, 1037, 3372, 3758
MteI	GCGCNGCGC	1 cut(s)	1494
MunI	CAATTG	3 cut(s)	77, 712, 1137
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	1 cut(s)	3758
MvnI	CGCG	1 cut(s)	1493
NciI	CCSGG	3 cut(s)	400, 1037, 3372
NcoI	CCATGG	2 cut(s)	3139, 3672
NdeI	CATATG	1 cut(s)	103
NheI	GCTAGC	1 cut(s)	2438
NlaIV	GGNNCC	8 cut(s)	594, 912, 944, 2252, 2307, 2358, 2917, 3494
NmeAIII	GCCGAG	1 cut(s)	3107
NsiI	ATGCAT	2 cut(s)	103, 855
NspI	RCATGY	3 cut(s)	219, 247, 804
NspV	TTCGAA	1 cut(s)	2516
PaeI	GCATGC	1 cut(s)	247
PciI	ACATGT	1 cut(s)	800
PcsI	WCGNNNNNNNCGW	1 cut(s)	2184
PctI	GAATGC	1 cut(s)	658
PdmI	GAANNNNTTC	1 cut(s)	2249
PfeI	GAWTC	8 cut(s)	34, 1337, 1502, 2362, 2395, 2513, 2631, 2698
PflFI	GACNNNGTC	1 cut(s)	227
PflMI	CCANNNNNTGG	1 cut(s)	3575
PfoI	TCCNGGA	2 cut(s)	1035, 3756
PleI	GAGTC	8 cut(s)	1109, 1128, 1553, 2012, 2063, 2501, 3163, 3812
PpsI	GAGTC	8 cut(s)	1109, 1128, 1553, 2012, 2063, 2501, 3163, 3812
PscI	ACATGT	1 cut(s)	800
Psp6I	CCWGG	1 cut(s)	3756
PspEI	GGTNACC	2 cut(s)	992, 1964
PspFI	CCCAGC	1 cut(s)	2557
PspGI	CCWGG	1 cut(s)	3756
PspN4I	GGNNCC	8 cut(s)	594, 912, 944, 2252, 2307, 2358, 2917, 3494
PspPI	GGNCC	7 cut(s)	401, 1622, 1985, 2104, 2300, 2523, 3754
PstI	CTGCAG	4 cut(s)	116, 876, 956, 1058
PstNI	CAGNNNCTG	2 cut(s)	614, 3128
PsuI	RGATCY	2 cut(s)	809, 1363
PsyI	GACNNNGTC	1 cut(s)	227
PvuII	CAGCTG	4 cut(s)	611, 1595, 3206, 3617
RsaI	GTAC	4 cut(s)	912, 944, 2288, 3684
RsaNI	GTAC	4 cut(s)	911, 943, 2287, 3683
RseI	CAYNNNNRTG	8 cut(s)	493, 2335, 2354, 2624, 3138, 3231, 3249, 3498
Rsr2I	CGGWCCG	1 cut(s)	2300
RsrII	CGGWCCG	1 cut(s)	2300
SalI	GTCGAC	2 cut(s)	2189, 3479
Sau96I	GGNCC	7 cut(s)	401, 1622, 1985, 2104, 2300, 2523, 3754
ScaI	AGTACT	1 cut(s)	3684
SchI	GAGTC	8 cut(s)	1110, 1129, 1553, 2012, 2064, 2501, 3164, 3812
ScrFI	CCNGG	4 cut(s)	400, 1037, 3372, 3758
SduI	GDGCHC	3 cut(s)	1383, 2330, 3727
SfcI	CTRYAG	4 cut(s)	112, 872, 952, 1054
SfuI	TTCGAA	1 cut(s)	2516
SgrAI	CRCCGGYG	1 cut(s)	1958
SinI	GGWCC	4 cut(s)	1622, 2104, 2300, 3754
SmiI	ATTTAAAT	1 cut(s)	786
SmiMI	CAYNNNNRTG	8 cut(s)	493, 2335, 2354, 2624, 3138, 3231, 3249, 3498
SmlI	CTYRAG	3 cut(s)	1891, 2143, 3070
SmoI	CTYRAG	3 cut(s)	1891, 2143, 3070
SpeI	ACTAGT	1 cut(s)	892
SphI	GCATGC	1 cut(s)	247
SspI	AATATT	1 cut(s)	1282
SspMI	CTAG	7 cut(s)	480, 845, 893, 1143, 1487, 2223, 2439
StyD4I	CCNGG	4 cut(s)	398, 1035, 3370, 3756
StyI	CCWWGG	6 cut(s)	921, 1476, 2806, 3139, 3672, 3718
SwaI	ATTTAAAT	1 cut(s)	786
TaiI	ACGT	7 cut(s)	414, 1412, 1475, 2190, 2674, 3031, 3079
TaqII	GACCGA	2 cut(s)	2288, 2388
TatI	WGTACW	1 cut(s)	3682
TauI	GCSGC	7 cut(s)	608, 1062, 1496, 1578, 1696, 1986, 2381
TfiI	GAWTC	8 cut(s)	34, 1337, 1502, 2362, 2395, 2513, 2631, 2698
TspGWI	ACGGA	6 cut(s)	441, 1349, 1802, 1849, 2117, 2450
Tth111I	GACNNNGTC	1 cut(s)	227
Van91I	CCANNNNNTGG	1 cut(s)	3575
VneI	GTGCAC	1 cut(s)	3723
VpaK11BI	GGWCC	4 cut(s)	1622, 2104, 2300, 3754
XbaI	TCTAGA	1 cut(s)	844
XceI	RCATGY	3 cut(s)	219, 247, 804
XmiI	GTMKAC	2 cut(s)	2190, 3480
XmnI	GAANNNNTTC	1 cut(s)	2249
XspI	CTAG	7 cut(s)	480, 845, 893, 1143, 1487, 2223, 2439
ZrmI	AGTACT	1 cut(s)	3684
Zsp2I	ATGCAT	2 cut(s)	103, 855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.