Rmu_ssc0000396.1_g000007

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000396.1
OG0011069
Physical Location & Seq
Forward (+)
52852 .. 53388
537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
atgagtataggaggaggaacagtagggttattaagctcagccacagccacagccacagccataggcagagctgcgtcgagtacaccagcagatgcggggcaaaggaattcacgattcccttgttgctgggcagcggcaggggcaaagctcactcgcgctcactacccaacaactccaactctaactccaactccaactccaactcctaggaggaaaagtagaaggattctttgcgaagtcgctatcggatccgatttatctccatcaacatcatcggaggtagaagcggccgcctcttcaaagatcggggctagggtcaaggtcaccgtgcctctcaaggtgtaccacgtgcccagagttcccgaggtcgacatcactggcatggaaggtgaactcaagcagtacgttggcctctggaagggcaaacggatttctgccaatctcccttataaagtacagttctttgtcgacgtcgaaggccgtggccctgttaaattctttgcgcatctcaaggaggacgagttcgaatacctttga

Protein Analysis

178

Amino Acids

19.03

Weight (kDa)

9.6

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 102 - 174 1.1e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AatII GACGTC 1 cut(s) 474
Acc16I TGCGCA 1 cut(s) 504
AccI GTMKAC 2 cut(s) 369, 468
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 95, 134, 287, 291
AclWI GGATC 2 cut(s) 243, 256
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 2 cut(s) 106, 494
AcvI CACGTG 1 cut(s) 349
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 4 cut(s) 82, 344, 404, 456
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 1 cut(s) 300
AloI GAACNNNNNNTCC 1 cut(s) 506
AluBI AGCT 3 cut(s) 36, 71, 148
AluI AGCT 3 cut(s) 36, 71, 148
AlwI GGATC 2 cut(s) 243, 256
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 4 cut(s) 288, 409, 478, 484
ApeKI GCWGC 2 cut(s) 71, 131
ApoI RAATTY 2 cut(s) 106, 494
AspA2I CCTAGG 1 cut(s) 206
AspLEI GCGC 2 cut(s) 158, 505
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 2 cut(s) 316, 401
AsuII TTCGAA 1 cut(s) 525
AvaI CYCGRG 1 cut(s) 362
AvrII CCTAGG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 354
BamHI GGATCC 1 cut(s) 248
BbrPI CACGTG 1 cut(s) 349
BbvI GCAGC 2 cut(s) 58, 143
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 465
BfaI CTAG 2 cut(s) 207, 312
BisI GCNGC 5 cut(s) 72, 132, 135, 288, 291
BlnI CCTAGG 1 cut(s) 206
BlpI GCTNAGC 1 cut(s) 37
BlsI GCNGC 5 cut(s) 73, 133, 136, 289, 292
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 250
BmsI GCATC 2 cut(s) 82, 514
Bpu1102I GCTNAGC 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 525
BpuEI CTTGAG 3 cut(s) 320, 380, 494
BsaAI YACGTR 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 3 cut(s) 206, 363, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 382
BseDI CCNNGG 3 cut(s) 206, 363, 481
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 354
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 58, 143
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 290, 411, 480, 486
BsiEI CGRYCG 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 418
BsnI GGCC 4 cut(s) 290, 411, 480, 486
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 2 cut(s) 248, 303
Bsp1720I GCTNAGC 1 cut(s) 37
BspACI CCGC 4 cut(s) 95, 134, 287, 291
BspANI GGCC 4 cut(s) 290, 411, 480, 486
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 250
BspPI GGATC 2 cut(s) 243, 256
BspT104I TTCGAA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 206, 363, 481
BssMI GATC 2 cut(s) 248, 303
BssNI GRCGYC 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 206
Bst4CI ACNGT 3 cut(s) 22, 328, 459
Bst6I CTCTTC 1 cut(s) 301
BstACI GRCGYC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 349
BstBI TTCGAA 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 481
BstEII GGTNACC 1 cut(s) 322
BstENI CCTNNNNNAGG 1 cut(s) 416
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 2 cut(s) 158, 505
BstKTI GATC 2 cut(s) 251, 306
BstMBI GATC 2 cut(s) 248, 303
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 322
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 58, 143
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BstZI CGGCCG 1 cut(s) 288
BsuRI GGCC 4 cut(s) 290, 411, 480, 486
BtgI CCRYGG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 375
CciNI GCGGCCGC 1 cut(s) 288
CfoI GCGC 2 cut(s) 158, 505
Cfr13I GGNCC 1 cut(s) 485
CseI GACGC 1 cut(s) 63
Csp6I GTAC 4 cut(s) 81, 343, 403, 455
CviAII CATG 1 cut(s) 382
CviQI GTAC 4 cut(s) 81, 343, 403, 455
DdeI CTNAG 1 cut(s) 37
DpnI GATC 2 cut(s) 250, 305
DpnII GATC 2 cut(s) 248, 303
EaeI YGGCCR 1 cut(s) 288
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 322
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO65I GGTNACC 1 cut(s) 322
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 1 cut(s) 385
FaiI YATR 4 cut(s) 8, 62, 383, 450
FatI CATG 1 cut(s) 381
FauI CCCGC 1 cut(s) 88
FblI GTMKAC 2 cut(s) 369, 468
Fnu4HI GCNGC 5 cut(s) 72, 132, 135, 288, 291
Fsp4HI GCNGC 5 cut(s) 72, 132, 135, 288, 291
FspBI CTAG 2 cut(s) 207, 312
FspI TGCGCA 1 cut(s) 504
GlaI GCGC 2 cut(s) 157, 504
GluI GCNGC 5 cut(s) 72, 132, 135, 288, 291
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 4 cut(s) 290, 411, 480, 486
HgaI GACGC 1 cut(s) 63
HhaI GCGC 2 cut(s) 158, 505
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 2 cut(s) 156, 503
HinP1I GCGC 2 cut(s) 156, 503
HincII GTYRAC 2 cut(s) 370, 469
HindII GTYRAC 2 cut(s) 370, 469
HinfI GANTC 2 cut(s) 114, 226
HphI GGTGA 2 cut(s) 316, 401
Hpy166II GTNNAC 5 cut(s) 83, 343, 370, 392, 469
Hpy188I TCNGA 3 cut(s) 248, 253, 277
Hpy188III TCNNGA 3 cut(s) 111, 362, 415
Hpy8I GTNNAC 5 cut(s) 83, 343, 370, 392, 469
Hpy99I CGWCG 3 cut(s) 79, 473, 476
HpyAV CCTTC 4 cut(s) 216, 380, 412, 470
HpyCH4III ACNGT 3 cut(s) 22, 328, 459
HpyCH4IV ACGT 3 cut(s) 348, 405, 471
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 3 cut(s) 348, 405, 471
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 2 cut(s) 156, 503
Kzo9I GATC 2 cut(s) 248, 303
LpnPI CCDG 7 cut(s) 99, 112, 123, 363, 367, 400, 501
Lsp1109I GCAGC 2 cut(s) 58, 143
LweI GCATC 2 cut(s) 82, 514
MaeI CTAG 2 cut(s) 207, 312
MaeII ACGT 3 cut(s) 348, 405, 471
MaeIII GTNAC 1 cut(s) 322
MalI GATC 2 cut(s) 250, 305
MboI GATC 2 cut(s) 248, 303
MboII GAAGA 1 cut(s) 288
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 2 cut(s) 106, 494
MmeI TCCRAC 4 cut(s) 200, 212, 218, 224
MnlI CCTC 9 cut(s) 5, 8, 204, 271, 304, 342, 358, 422, 508
MseI TTAA 2 cut(s) 32, 492
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 134
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 248, 303
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 322
NotI GCGGCCGC 1 cut(s) 288
NsbI TGCGCA 1 cut(s) 504
NspV TTCGAA 1 cut(s) 525
PfeI GAWTC 2 cut(s) 114, 226
PkrI GCNGC 5 cut(s) 73, 133, 136, 289, 292
PmaCI CACGTG 1 cut(s) 349
PmlI CACGTG 1 cut(s) 349
Ppu21I YACGTR 1 cut(s) 349
PsiI TTATAA 1 cut(s) 450
PspCI CACGTG 1 cut(s) 349
PspEI GGTNACC 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 1 cut(s) 485
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 4 cut(s) 82, 344, 404, 456
RsaNI GTAC 4 cut(s) 81, 343, 403, 455
RseI CAYNNNNRTG 1 cut(s) 380
SalI GTCGAC 2 cut(s) 368, 467
SaqAI TTAA 2 cut(s) 32, 492
SatI GCNGC 5 cut(s) 72, 132, 135, 288, 291
Sau3AI GATC 2 cut(s) 248, 303
Sau96I GGNCC 1 cut(s) 485
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 2 cut(s) 82, 514
SfuI TTCGAA 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 335, 395, 509
SmoI CTYRAG 3 cut(s) 335, 395, 509
Sse9I AATT 2 cut(s) 106, 494
SsiI CCGC 4 cut(s) 95, 134, 287, 291
SspMI CTAG 2 cut(s) 207, 312
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 3 cut(s) 22, 328, 459
TaiI ACGT 3 cut(s) 351, 408, 474
TaqI TCGA 5 cut(s) 77, 369, 468, 474, 525
TasI AATT 2 cut(s) 106, 494
TatI WGTACW 2 cut(s) 80, 454
TauI GCSGC 3 cut(s) 137, 290, 293
TfiI GAWTC 2 cut(s) 114, 226
Tru1I TTAA 2 cut(s) 32, 492
Tru9I TTAA 2 cut(s) 32, 492
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 2 cut(s) 71, 131
Tsp45I GTSAC 1 cut(s) 322
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 2 cut(s) 106, 494
XmaJI CCTAGG 1 cut(s) 206
XmiI GTMKAC 2 cut(s) 369, 468
XspI CTAG 2 cut(s) 207, 312
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.