Rmu_ssc0000434.1_g000045

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000434.1
OG0014413
Physical Location & Seq
Reverse (-)
197186 .. 198184
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000434.1_g000045.1.cds

Sequence Viewer

Length: 999 bp
atgaggtgcaagaagcacgccgccgacctcacaagcgccgtcggcgtctgcgcctcttgtctccgggaacgactgaccgccatcatcgaggcccagacccgagcccaggcccaattacaactccaggctcagctctcccgcctccactcccgcagcaacggcgtcgctcccgaagaacccctcaagtccgaccccaaccccccgccgctcatcttcccccgctccgtctccccctacgtttcccgccgcaaatccgacgactccgcctggccgcaccaccacaatcgctccgatcacgaccacgaccacaaccaccagcgccatcatatccgcttctacagtactccccaggtggggcctacctacgacggaggagccgccactatcggaggctcctacaggaagagcaagcccaagttttcgctcctgacgagtctgttccggtccagatccgacaagttctggacggatcctagggtttcgtcgcgggactcgacttcgacgtcggcgtcgtccgcgccgtcgccgtcgtggttctcgacgatcttcgccgggaaaagagggaagcaatcgaagcaattctactccgacgagtccacgatccgcgtgagatctcgccacagaagagcgcgcggaatgtcgccggatttaacgcacgacgccgccggcggtggctgcgaggacgactgcgaccgattggcctccggcagcggggactcatcgacgccggattggaagaggactccggtgcaggccacgccgtcgcggcggacgcgttcgggtccggttaagaatgtgtcgggtctgacgttctgcctgagcccgctggttcgggccagcccgaacccgcactggaaccagaagtgcatgcagggtttcccggctgaggttggcgaggtcagggtggcgacgaagccacacttggccacagcggcgtcgtttagcatgaaccggtcgaggaagctggcagatatcggtagagccacttccaaccgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

332

Amino Acids

36.62

Weight (kDa)

10.35

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44600 AT3G60200
fragaria_vesca FvH4_7g08720
malus_domestica MD07G1088100.v1.1
prunus_persica Prupe.2G114900_v2.0.a1
pyrus_communis pycom02g19430 pycom07g07070
rosa_laevigata RLG00000028945 RLG00000030787
rosa_multiflora Rmu_ssc0000434.1_g000045
rosa_roxburghii Rroxscaffold_4G00310220
rosa_rugosa Rorug01G0169900
rosa_samantha Rh1BG152800 Rh1DG185200 Rh5DG492500 Rh6AG022200
rosa_wichuraiana Rw1G015380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 502, 508
AatII GACGTC 1 cut(s) 506
AccBSI CCGCTC 2 cut(s) 208, 222
AccII CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
AclWI GGATC 4 cut(s) 444, 464, 477, 595
AcoI YGGCCR 2 cut(s) 269, 924
AcyI GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 5 cut(s) 106, 353, 354, 711, 886
AflIII ACRYGT 1 cut(s) 773
AgeI ACCGGT 1 cut(s) 951
AjnI CCWGG 4 cut(s) 105, 123, 266, 348
AjuI GAANNNNNNNTTGG 2 cut(s) 905, 937
AluBI AGCT 2 cut(s) 133, 964
AluI AGCT 2 cut(s) 133, 964
Alw26I GTCTC 2 cut(s) 65, 232
AlwI GGATC 4 cut(s) 444, 464, 477, 595
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 8 cut(s) 90, 108, 269, 356, 699, 753, 834, 924
ApeKI GCWGC 3 cut(s) 153, 675, 708
AsiGI ACCGGT 1 cut(s) 951
Asp700I GAANNNNTTC 1 cut(s) 578
AspA2I CCTAGG 1 cut(s) 473
AspLEI GCGC 6 cut(s) 38, 53, 321, 520, 631, 633
AspS9I GGNCC 6 cut(s) 91, 109, 356, 444, 782, 834
AsuC2I CCSGG 3 cut(s) 65, 553, 881
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 2 cut(s) 444, 782
AvrII CCTAGG 1 cut(s) 473
BalI TGGCCA 1 cut(s) 926
BamHI GGATCC 1 cut(s) 469
BanII GRGCYC 2 cut(s) 106, 824
BarI GAAGNNNNNNTAC 3 cut(s) 566, 598, 970
BbvCI CCTCAGC 1 cut(s) 885
BbvI GCAGC 3 cut(s) 165, 662, 720
BccI CCATC 2 cut(s) 89, 330
BceAI ACGGC 5 cut(s) 23, 175, 505, 511, 745
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 4 cut(s) 107, 125, 268, 350
BcnI CCSGG 3 cut(s) 65, 553, 881
BcoDI GTCTC 2 cut(s) 65, 232
BfaI CTAG 1 cut(s) 474
BfmI CTRYAG 2 cut(s) 337, 397
BfoI RGCGCY 2 cut(s) 39, 322
BglI GCCNNNNNGGC 2 cut(s) 766, 932
BglII AGATCT 1 cut(s) 611
BlnI CCTAGG 1 cut(s) 473
BlpI GCTNAGC 1 cut(s) 129
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 7 cut(s) 65, 107, 125, 268, 350, 553, 881
Bme18I GGWCC 2 cut(s) 444, 782
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 6 cut(s) 91, 109, 356, 444, 782, 834
BmiI GGNNCC 6 cut(s) 357, 376, 394, 471, 783, 857
BmrFI CCNGG 7 cut(s) 65, 107, 125, 268, 350, 553, 881
BpmI CTGGAG 1 cut(s) 107
Bpu10I CCTNAGC 2 cut(s) 818, 885
Bpu1102I GCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 3 cut(s) 65, 553, 881
BsaHI GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
BsaJI CCNNGG 3 cut(s) 105, 348, 473
BsaWI WCCGGW 4 cut(s) 441, 745, 784, 951
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 353, 354, 711, 886
Bse118I RCCGGY 2 cut(s) 665, 951
Bse1I ACTGG 1 cut(s) 857
BseBI CCWGG 4 cut(s) 107, 125, 268, 350
BseDI CCNNGG 3 cut(s) 105, 348, 473
BseLI CCNNNNNNNGG 5 cut(s) 106, 353, 354, 711, 886
BseMII CTCAG 3 cut(s) 143, 809, 876
BseNI ACTGG 1 cut(s) 857
BsePI GCGCGC 1 cut(s) 629
BseRI GAGGAG 1 cut(s) 387
BseXI GCAGC 3 cut(s) 165, 662, 720
BsgI GTGCAG 1 cut(s) 770
Bsh1236I CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
Bsh1285I CGRYCG 2 cut(s) 694, 956
BshFI GGCC 8 cut(s) 92, 110, 271, 358, 701, 755, 836, 926
BshTI ACCGGT 1 cut(s) 951
BsiEI CGRYCG 2 cut(s) 694, 956
BsiHKCI CYCGRG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 503, 728
BslI CCNNNNNNNGG 5 cut(s) 106, 353, 354, 711, 886
BsmAI GTCTC 2 cut(s) 65, 232
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 2 cut(s) 503, 728
BsnI GGCC 8 cut(s) 92, 110, 271, 358, 701, 755, 836, 926
BsoBI CYCGRG 1 cut(s) 99
Bsp1286I GDGCHC 2 cut(s) 106, 824
Bsp143I GATC 6 cut(s) 292, 449, 469, 543, 600, 611
Bsp1720I GCTNAGC 1 cut(s) 129
BspANI GGCC 8 cut(s) 92, 110, 271, 358, 701, 755, 836, 926
BspCNI CTCAG 3 cut(s) 142, 810, 877
BspFNI CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
BspLI GGNNCC 6 cut(s) 357, 376, 394, 471, 783, 857
BspPI GGATC 4 cut(s) 444, 464, 477, 595
BspQI GCTCTTC 2 cut(s) 398, 619
BsrBI CCGCTC 2 cut(s) 208, 222
BsrFI RCCGGY 2 cut(s) 665, 951
BsrI ACTGG 1 cut(s) 857
BssAI RCCGGY 2 cut(s) 665, 951
BssECI CCNNGG 3 cut(s) 105, 348, 473
BssHII GCGCGC 1 cut(s) 629
BssMI GATC 6 cut(s) 292, 449, 469, 543, 600, 611
BssNI GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 4 cut(s) 107, 125, 268, 350
Bst4CI ACNGT 2 cut(s) 341, 995
Bst6I CTCTTC 3 cut(s) 398, 619, 731
BstACI GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
BstC8I GCNNGC 9 cut(s) 18, 410, 631, 667, 753, 824, 838, 869, 966
BstDEI CTNAG 3 cut(s) 129, 818, 885
BstFNI CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
BstH2I RGCGCY 2 cut(s) 39, 322
BstHHI GCGC 6 cut(s) 38, 53, 321, 520, 631, 633
BstKTI GATC 6 cut(s) 295, 452, 472, 546, 603, 614
BstMAI GTCTC 2 cut(s) 65, 232
BstMBI GATC 6 cut(s) 292, 449, 469, 543, 600, 611
BstMCI CGRYCG 2 cut(s) 694, 956
BstMWI GCNNNNNNNGC 9 cut(s) 42, 159, 515, 574, 675, 757, 766, 772, 932
BstNI CCWGG 4 cut(s) 107, 125, 268, 350
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 7 cut(s) 63, 105, 123, 266, 348, 551, 879
BstSFI CTRYAG 2 cut(s) 337, 397
BstUI CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
BstV1I GCAGC 3 cut(s) 165, 662, 720
BstX2I RGATCY 3 cut(s) 449, 469, 611
BstYI RGATCY 3 cut(s) 449, 469, 611
BsuRI GGCC 8 cut(s) 92, 110, 271, 358, 701, 755, 836, 926
BtsIMutI CAGTG 1 cut(s) 850
Cac8I GCNNGC 9 cut(s) 18, 410, 631, 667, 753, 824, 838, 869, 966
CfoI GCGC 6 cut(s) 38, 53, 321, 520, 631, 633
Cfr10I RCCGGY 2 cut(s) 665, 951
Cfr13I GGNCC 6 cut(s) 91, 109, 356, 444, 782, 834
CseI GACGC 7 cut(s) 34, 151, 498, 668, 733, 781, 924
Csp6I GTAC 1 cut(s) 342
CspAI ACCGGT 1 cut(s) 951
CviAII CATG 2 cut(s) 868, 946
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 3 cut(s) 129, 818, 885
DpnI GATC 6 cut(s) 294, 451, 471, 545, 602, 613
DpnII GATC 6 cut(s) 292, 449, 469, 543, 600, 611
DrdI GACNNNNNNGTC 2 cut(s) 502, 508
DseDI GACNNNNNNGTC 2 cut(s) 502, 508
EaeI YGGCCR 2 cut(s) 269, 924
Eam1104I CTCTTC 3 cut(s) 398, 619, 731
EarI CTCTTC 3 cut(s) 398, 619, 731
EciI GGCGGA 2 cut(s) 253, 784
Eco130I CCWWGG 1 cut(s) 473
Eco24I GRGCYC 2 cut(s) 106, 824
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 2 cut(s) 444, 782
Eco88I CYCGRG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 4 cut(s) 105, 123, 266, 348
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 473
EcoT38I GRGCYC 2 cut(s) 106, 824
ErhI CCWWGG 1 cut(s) 473
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 2 cut(s) 871, 949
FaiI YATR 3 cut(s) 327, 869, 947
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FaqI GGGAC 2 cut(s) 503, 728
FatI CATG 2 cut(s) 867, 945
FauI CCCGC 9 cut(s) 146, 158, 210, 227, 251, 480, 704, 831, 855
FriOI GRGCYC 2 cut(s) 106, 824
FspBI CTAG 1 cut(s) 474
GlaI GCGC 6 cut(s) 37, 52, 320, 519, 630, 632
GsuI CTGGAG 1 cut(s) 107
HaeII RGCGCY 2 cut(s) 39, 322
HaeIII GGCC 8 cut(s) 92, 110, 271, 358, 701, 755, 836, 926
HgaI GACGC 7 cut(s) 34, 151, 498, 668, 733, 781, 924
HhaI GCGC 6 cut(s) 38, 53, 321, 520, 631, 633
Hin1I GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
Hin1II CATG 2 cut(s) 871, 949
Hin6I GCGC 6 cut(s) 36, 51, 319, 518, 629, 631
HinP1I GCGC 6 cut(s) 36, 51, 319, 518, 629, 631
HinfI GANTC 6 cut(s) 260, 433, 491, 593, 716, 742
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 7 cut(s) 190, 256, 292, 389, 454, 589, 807
Hpy188III TCNNGA 6 cut(s) 170, 296, 427, 447, 463, 538
Hpy8I GTNNAC 1 cut(s) 597
HpyCH4III ACNGT 2 cut(s) 341, 995
HpyCH4IV ACGT 3 cut(s) 237, 503, 809
HpyCH4V TGCA 4 cut(s) 9, 751, 867, 871
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 159, 515, 574, 675, 757, 766, 772, 932
HpyF3I CTNAG 3 cut(s) 129, 818, 885
HpySE526I ACGT 3 cut(s) 237, 503, 809
Hsp92I GRCGYC 7 cut(s) 45, 162, 503, 509, 660, 725, 935
Hsp92II CATG 2 cut(s) 871, 949
HspAI GCGC 6 cut(s) 36, 51, 319, 518, 629, 631
KroI GCCGGC 1 cut(s) 665
KroNI GCCGGC 1 cut(s) 667
Kzo9I GATC 6 cut(s) 292, 449, 469, 543, 600, 611
LguI GCTCTTC 2 cut(s) 398, 619
LmnI GCTCC 6 cut(s) 172, 227, 293, 374, 398, 429
Lsp1109I GCAGC 3 cut(s) 165, 662, 720
MaeI CTAG 1 cut(s) 474
MaeII ACGT 3 cut(s) 237, 503, 809
MalI GATC 6 cut(s) 294, 451, 471, 545, 602, 613
MbiI CCGCTC 2 cut(s) 208, 222
MboI GATC 6 cut(s) 292, 449, 469, 543, 600, 611
MboII GAAGA 6 cut(s) 185, 205, 415, 538, 636, 748
MflI RGATCY 3 cut(s) 449, 469, 611
MhlI GDGCHC 2 cut(s) 106, 824
MlsI TGGCCA 1 cut(s) 926
MluCI AATT 2 cut(s) 113, 578
MluI ACGCGT 1 cut(s) 773
MluNI TGGCCA 1 cut(s) 926
MlyI GAGTC 6 cut(s) 254, 442, 485, 602, 710, 736
MmeI TCCRAC 4 cut(s) 213, 279, 477, 612
Mox20I TGGCCA 1 cut(s) 926
MreI CGCCGGCG 1 cut(s) 665
MroNI GCCGGC 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 578
MscI TGGCCA 1 cut(s) 926
MseI TTAA 2 cut(s) 650, 789
Msp20I TGGCCA 1 cut(s) 926
MspA1I CMGCKG 3 cut(s) 711, 826, 932
MspR9I CCNGG 7 cut(s) 65, 107, 125, 268, 350, 553, 881
MvaI CCWGG 4 cut(s) 107, 125, 268, 350
MvnI CGCG 7 cut(s) 487, 518, 606, 631, 633, 766, 775
MwoI GCNNNNNNNGC 9 cut(s) 42, 159, 515, 574, 675, 757, 766, 772, 932
NaeI GCCGGC 1 cut(s) 667
NciI CCSGG 3 cut(s) 65, 553, 881
NdeII GATC 6 cut(s) 292, 449, 469, 543, 600, 611
NgoMIV GCCGGC 1 cut(s) 665
NlaIII CATG 2 cut(s) 871, 949
NlaIV GGNNCC 6 cut(s) 357, 376, 394, 471, 783, 857
NspI RCATGY 1 cut(s) 871
PaeI GCATGC 1 cut(s) 871
PauI GCGCGC 1 cut(s) 629
PciSI GCTCTTC 2 cut(s) 398, 619
PcsI WCGNNNNNNNCGW 8 cut(s) 428, 491, 500, 506, 509, 518, 536, 806
PdiI GCCGGC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 63
PinAI ACCGGT 1 cut(s) 951
PleI GAGTC 6 cut(s) 254, 441, 485, 601, 710, 736
PpsI GAGTC 6 cut(s) 254, 441, 485, 601, 710, 736
Psp6I CCWGG 4 cut(s) 105, 123, 266, 348
PspGI CCWGG 4 cut(s) 105, 123, 266, 348
PspN4I GGNNCC 6 cut(s) 357, 376, 394, 471, 783, 857
PspPI GGNCC 6 cut(s) 91, 109, 356, 444, 782, 834
PsuI RGATCY 3 cut(s) 449, 469, 611
PteI GCGCGC 1 cut(s) 629
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
SapI GCTCTTC 2 cut(s) 398, 619
SaqAI TTAA 2 cut(s) 650, 789
Sau3AI GATC 6 cut(s) 292, 449, 469, 543, 600, 611
Sau96I GGNCC 6 cut(s) 91, 109, 356, 444, 782, 834
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 6 cut(s) 254, 442, 485, 602, 710, 736
ScrFI CCNGG 7 cut(s) 65, 107, 125, 268, 350, 553, 881
SduI GDGCHC 2 cut(s) 106, 824
SfcI CTRYAG 2 cut(s) 337, 397
SgrAI CRCCGGYG 1 cut(s) 665
SinI GGWCC 2 cut(s) 444, 782
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SphI GCATGC 1 cut(s) 871
Sse9I AATT 2 cut(s) 113, 578
SspMI CTAG 1 cut(s) 474
StyD4I CCNGG 7 cut(s) 63, 105, 123, 266, 348, 551, 879
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 2 cut(s) 341, 995
TaiI ACGT 3 cut(s) 240, 506, 812
TaqI TCGA 7 cut(s) 87, 494, 500, 539, 572, 722, 956
TaqII GACCGA 1 cut(s) 708
TasI AATT 2 cut(s) 113, 578
TatI WGTACW 1 cut(s) 341
TauI GCSGC 8 cut(s) 23, 208, 249, 274, 380, 665, 769, 935
Tru1I TTAA 2 cut(s) 650, 789
Tru9I TTAA 2 cut(s) 650, 789
TscAI CASTG 1 cut(s) 857
TseI GCWGC 3 cut(s) 153, 675, 708
TspDTI ATGAA 1 cut(s) 962
TspGWI ACGGA 3 cut(s) 214, 384, 482
TspRI CASTG 1 cut(s) 857
VpaK11BI GGWCC 2 cut(s) 444, 782
XceI RCATGY 1 cut(s) 871
XmaJI CCTAGG 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 1 cut(s) 474
ZraI GACGTC 1 cut(s) 504
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.