Rmu_ssc0000467.1_g000036

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000467.1
OG0012187
Physical Location & Seq
Forward (+)
120669 .. 123155
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1281 bp
atgtcggcggtgccgccgcccgtttctccctcccgcaccacctgcgcttctctcctccatgaactacagatcatttgggacgaaattggggagagtgacagtgagagggacaccatgcttctccaactcgagcaagaatgcctcgacatctatcggaagaaggtggaaatgacgagaaagtacagggctgatttgcacaagtccttagccgacggccaggctcaaatcgccgccatcgcctccgctctcggagaacctaattcgtttccaagggcaagagggaccctcagggaccagatatccgccgtgaagcctgttttggacgaactgagcttgaagaggcaggaaagggtgagggacttttcggtaattcaaacacagattgcgaggatttcgtctgaaatagctgggaaaggcctggctaagaatgccggtgaggatgccggagtggatgagcgtgatttgactgtgaagaggttgggagagcttaagtcccaccttgacgaacttcacaaagaaaaggctttacgtttacagaaggtcaatagtcatgtcaataccatccatgacctctcagttgtaatgtccattgatttctttaggacactcgaagaagtccacccaagcttgagtgatcagtccgccgctcattccaagagcatcagcaatgacactcttgctagattgaccgcactgaccaactcgctgaagcaagagaaacagcagagactgcaaaagctacaagagcttggaagcaagctgatagaagtatggaatctcatggagacaccaactgaggaacggaacaaatttgaccatgttacacgcttaatttcttcctcagttgatgagctgtcaaggcatggatgcctttctctagatgtcattgagcagactgaggctgaagtcgagcggttgaatgttttgaaagccagcaagatgaaggagttggtctttaagaggcaaaatgagctcgaagaactttatagaggagtccacatggatgtagatagtgatggagctagacagattctcagcagcctcatagattctggtaatgttaacttgtctgatctgctttcaagcatggatgatcagattgtaaaggcaaaagagcaagctttaagcagaaaggatattttggataaggtagagaaatgcaaatttgcatttgaggaggacaagtggcttgatgagtatgaaaaggtaaaaatatttgagaaagtgttacaatttaatatttttagtttggtacatctatttgtttcctctgacttatga

Protein Analysis

426

Amino Acids

48.3

Weight (kDa)

5.53

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 17 - 398 3.1e-46 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 3 cut(s) 245, 647, 913
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 2 cut(s) 809, 1163
AcuI CTGAAG 2 cut(s) 728, 924
AfaI GTAC 2 cut(s) 182, 1254
AflII CTTAAG 1 cut(s) 488
AgsI TTSAA 5 cut(s) 337, 374, 919, 928, 1083
AjnI CCWGG 2 cut(s) 216, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 2 cut(s) 721, 779
AlwNI CAGNNNCTG 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 214, 415
ApeKI GCWGC 1 cut(s) 1038
ApoI RAATTY 2 cut(s) 809, 1163
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 282, 292
AsuHPI GGTGA 2 cut(s) 364, 446
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 282, 292
AxyI CCTNAGG 1 cut(s) 287
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 975
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 1 cut(s) 1050
BccI CCATC 3 cut(s) 242, 569, 1010
BceAI ACGGC 2 cut(s) 229, 290
BciT130I CCWGG 2 cut(s) 218, 419
BclI TGATCA 2 cut(s) 634, 1093
BcoDI GTCTC 2 cut(s) 721, 779
BfaI CTAG 3 cut(s) 681, 878, 1023
BfmI CTRYAG 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 14, 17, 231, 645, 1039
BlsI GCNGC 5 cut(s) 15, 18, 232, 646, 1040
Bme1390I CCNGG 2 cut(s) 218, 419
Bme18I GGWCC 2 cut(s) 282, 292
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 282, 292
BmiI GGNNCC 4 cut(s) 12, 283, 284, 293
BmrFI CCNGG 2 cut(s) 218, 419
BmsI GCATC 3 cut(s) 430, 669, 857
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu10I CCTNAGC 1 cut(s) 205
BpuEI CTTGAG 1 cut(s) 649
BsaJI CCNNGG 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 1169, 1199
Bse118I RCCGGY 1 cut(s) 431
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 673
BseBI CCWGG 2 cut(s) 218, 419
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 6 cut(s) 445, 457, 561, 872, 1009, 1096
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 7 cut(s) 301, 320, 588, 786, 855, 888, 1048
BseRI GAGGAG 3 cut(s) 44, 1005, 1190
BseXI GCAGC 1 cut(s) 1050
BseYI CCCAGC 1 cut(s) 407
BshFI GGCC 2 cut(s) 216, 417
BshNI GGYRCC 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 975
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 432, 444
BslFI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 478
BsmAI GTCTC 2 cut(s) 721, 779
BsmFI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 478
BsmI GAATGC 2 cut(s) 143, 433
BsnI GGCC 2 cut(s) 216, 417
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 975
Bsp143I GATC 4 cut(s) 69, 634, 1072, 1093
BspANI GGCC 2 cut(s) 216, 417
BspCNI CTCAG 7 cut(s) 300, 321, 587, 787, 854, 889, 1047
BspLI GGNNCC 4 cut(s) 12, 283, 284, 293
BspMI ACCTGC 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 10
BspTI CTTAAG 1 cut(s) 488
BsrBI CCGCTC 3 cut(s) 245, 647, 913
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 4 cut(s) 69, 634, 1072, 1093
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 218, 419
Bst4CI ACNGT 2 cut(s) 101, 469
Bst6I CTCTTC 2 cut(s) 332, 467
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 42, 730
BstC8I GCNNGC 3 cut(s) 758, 934, 1119
BstDEI CTNAG 9 cut(s) 205, 287, 329, 423, 574, 795, 841, 897, 1034
BstF5I GGATG 6 cut(s) 445, 457, 561, 872, 1009, 1096
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 4 cut(s) 72, 637, 1075, 1096
BstMAI GTCTC 2 cut(s) 721, 779
BstMBI GATC 4 cut(s) 69, 634, 1072, 1093
BstMWI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 970
BstNI CCWGG 2 cut(s) 218, 419
BstSCI CCNGG 2 cut(s) 216, 417
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 1050
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 2 cut(s) 216, 417
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 6 cut(s) 445, 457, 561, 872, 1009, 1096
BtsIMutI CAGTG 2 cut(s) 106, 692
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 3 cut(s) 758, 934, 1119
CaiI CAGNNNCTG 1 cut(s) 730
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 2 cut(s) 282, 292
Csp6I GTAC 2 cut(s) 181, 1253
CviAII CATG 9 cut(s) 59, 115, 551, 566, 781, 818, 863, 1000, 1087
CviQI GTAC 2 cut(s) 181, 1253
DdeI CTNAG 9 cut(s) 205, 287, 329, 423, 574, 795, 841, 897, 1034
DpnI GATC 4 cut(s) 71, 636, 1074, 1095
DpnII GATC 4 cut(s) 69, 634, 1072, 1093
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 332, 467
EarI CTCTTC 2 cut(s) 332, 467
EciI GGCGGA 2 cut(s) 292, 631
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 975
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 282, 292
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 2 cut(s) 728, 924
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 216, 417
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 9 cut(s) 62, 118, 554, 569, 784, 821, 866, 1003, 1090
FaqI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 478
FatI CATG 9 cut(s) 58, 114, 550, 565, 780, 817, 862, 999, 1086
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 2 cut(s) 634, 1093
Fnu4HI GCNGC 5 cut(s) 14, 17, 231, 645, 1039
FokI GGATG 6 cut(s) 452, 464, 548, 879, 1016, 1103
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 5 cut(s) 14, 17, 231, 645, 1039
FspBI CTAG 3 cut(s) 681, 878, 1023
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 14, 17, 231, 645, 1039
GsaI CCCAGC 1 cut(s) 411
HaeIII GGCC 2 cut(s) 216, 417
HapII CCGG 2 cut(s) 432, 444
HhaI GCGC 1 cut(s) 47
Hin1II CATG 9 cut(s) 62, 118, 554, 569, 784, 821, 866, 1003, 1090
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HindIII AAGCTT 2 cut(s) 625, 1119
HinfI GANTC 4 cut(s) 775, 993, 1030, 1049
HpaI GTTAAC 1 cut(s) 1063
HpaII CCGG 2 cut(s) 432, 444
HphI GGTGA 2 cut(s) 364, 446
Hpy166II GTNNAC 4 cut(s) 533, 619, 997, 1063
Hpy188I TCNGA 6 cut(s) 156, 251, 400, 1072, 1098, 1273
Hpy188III TCNNGA 1 cut(s) 878
Hpy8I GTNNAC 4 cut(s) 533, 619, 997, 1063
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 154, 532, 937
HpyCH4III ACNGT 2 cut(s) 101, 469
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 4 cut(s) 196, 733, 1161, 1169
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 970
HpyF3I CTNAG 9 cut(s) 205, 287, 329, 423, 574, 795, 841, 897, 1034
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 9 cut(s) 62, 118, 554, 569, 784, 821, 866, 1003, 1090
HspAI GCGC 1 cut(s) 45
KflI GGGWCCC 1 cut(s) 282
Ksp22I TGATCA 2 cut(s) 634, 1093
KspAI GTTAAC 1 cut(s) 1063
Kzo9I GATC 4 cut(s) 69, 634, 1072, 1093
LmnI GCTCC 1 cut(s) 1019
Lsp1109I GCAGC 1 cut(s) 1050
LweI GCATC 3 cut(s) 430, 669, 857
MaeI CTAG 3 cut(s) 681, 878, 1023
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 3 cut(s) 95, 820, 1227
MalI GATC 4 cut(s) 71, 636, 1074, 1095
MbiI CCGCTC 3 cut(s) 245, 647, 913
MboI GATC 4 cut(s) 69, 634, 1072, 1093
MboII GAAGA 6 cut(s) 169, 349, 484, 623, 828, 989
MhlI GDGCHC 1 cut(s) 975
MluCI AATT 7 cut(s) 84, 259, 369, 809, 831, 1163, 1232
MlyI GAGTC 1 cut(s) 1002
MmeI TCCRAC 1 cut(s) 148
MseI TTAA 6 cut(s) 489, 830, 957, 1062, 1124, 1236
MslI CAYNNNNRTG 1 cut(s) 1002
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 2 cut(s) 432, 444
MspR9I CCNGG 2 cut(s) 218, 419
Mva1269I GAATGC 2 cut(s) 143, 433
MvaI CCWGG 2 cut(s) 218, 419
MwoI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 970
NdeII GATC 4 cut(s) 69, 634, 1072, 1093
NlaIII CATG 9 cut(s) 62, 118, 554, 569, 784, 821, 866, 1003, 1090
NlaIV GGNNCC 4 cut(s) 12, 283, 284, 293
NmuCI GTSAC 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 50
PceI AGGCCT 1 cut(s) 417
PctI GAATGC 2 cut(s) 143, 433
PfeI GAWTC 3 cut(s) 775, 1030, 1049
PkrI GCNGC 5 cut(s) 15, 18, 232, 646, 1040
PleI GAGTC 1 cut(s) 1001
PpsI GAGTC 1 cut(s) 1001
PpuMI RGGWCCY 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 216, 417
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 2 cut(s) 216, 417
PspN4I GGNNCC 4 cut(s) 12, 283, 284, 293
PspPI GGNCC 2 cut(s) 282, 292
PspPPI RGGWCCY 1 cut(s) 282
PspXI VCTCGAGB 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 730
RsaI GTAC 2 cut(s) 182, 1254
RsaNI GTAC 2 cut(s) 181, 1253
RseI CAYNNNNRTG 1 cut(s) 1002
SacI GAGCTC 1 cut(s) 975
SaqAI TTAA 6 cut(s) 489, 830, 957, 1062, 1124, 1236
SatI GCNGC 5 cut(s) 14, 17, 231, 645, 1039
Sau3AI GATC 4 cut(s) 69, 634, 1072, 1093
Sau96I GGNCC 2 cut(s) 282, 292
SchI GAGTC 1 cut(s) 1002
ScrFI CCNGG 2 cut(s) 218, 419
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 3 cut(s) 430, 669, 857
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 2 cut(s) 282, 292
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 3 cut(s) 128, 488, 628
SmoI CTYRAG 3 cut(s) 128, 488, 628
Sse9I AATT 7 cut(s) 84, 259, 369, 809, 831, 1163, 1232
SseBI AGGCCT 1 cut(s) 417
SspI AATATT 2 cut(s) 1215, 1240
SspMI CTAG 3 cut(s) 681, 878, 1023
SstI GAGCTC 1 cut(s) 975
StuI AGGCCT 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 216, 417
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 101, 469
TaiI ACGT 1 cut(s) 532
TaqI TCGA 5 cut(s) 129, 144, 609, 909, 975
TasI AATT 7 cut(s) 84, 259, 369, 809, 831, 1163, 1232
TatI WGTACW 1 cut(s) 180
TauI GCSGC 4 cut(s) 16, 19, 233, 647
TfiI GAWTC 3 cut(s) 775, 1030, 1049
Tru1I TTAA 6 cut(s) 489, 830, 957, 1062, 1124, 1236
Tru9I TTAA 6 cut(s) 489, 830, 957, 1062, 1124, 1236
TscAI CASTG 2 cut(s) 106, 699
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 1038
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 75, 956, 1215
TspGWI ACGGA 1 cut(s) 817
TspRI CASTG 2 cut(s) 106, 699
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 2 cut(s) 282, 292
XapI RAATTY 2 cut(s) 809, 1163
XbaI TCTAGA 1 cut(s) 877
XhoI CTCGAG 1 cut(s) 128
XspI CTAG 3 cut(s) 681, 878, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.