Rroxscaffold_1G00031180

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013178
Physical Location & Seq
Reverse (-)
43771195 .. 43771983
789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031180.1

Sequence Viewer

Length: 789 bp
ATGGCCGACCGAGTCCACCCGGGCGATTCGCCGGCTGCCCAGACGGCGCCGTTGCATGCCTCCAACGACAACAACAGCAACTCTGTCCTCCCTCAGCCGGGGACCTACGTGGTCCAAATCCCCAAGGACCAAGTCTACCGCATCCCACCGCCGGAGAACGCCACCCTCTACAAGAACTACACCCGCCGAAAGCCTCGCCGCAACTGCTGCCGCGTCTGCTTCTGCTGGATCCTCGGCATCCTTGCCTCTCTCATCCTCCTCTCCGCCATCGCCGCCGCCGTCTTCTACCTCGTCGTCCGCCCCGAGTCCCCCAACTACTCCGTCCAAAAAATCTCATTCAAAGGCTTCAACGTCACCGCCGCGTCGTCGTCGTCGACCATCTCGCCGGTGATCGACGTGACCGTGCGTGCGGATAACCCTAACAGGAAAATCGGGATCTACTACGAGCGGGACAGCTCGGCCAACGTGTTCTACACTGACATCCGGTTGTGTGACGGCGCTCTGCCGGCGTTTTATCAGCCGCCGAAGAACGTGACGGAGTTGAAAACGGTGCTGACGGGGAGCGGGATCAGGCTGACGAGCGCGGTGCAAAACGGGTTGGTGGAGGCGCAGAGCCAAGGCAAGGTGGCGTTACTTCTGCATGTCCGAGCCCCCGTTAGAATCAAAGTGGGGTCCATTAAGACGTGGACGATCACTGTTAAAGTTGACTGTGATTTAACGGTGGATAAGCTGACCTCAGACGCCAAGATTGTTTCTAAAGACTGCAACTACGGTGTGGATCCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

262

Amino Acids

28.71

Weight (kDa)

9.3

Isoelectric Point (pI)

33.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 134 - 237 9.3e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27080 AT2G27080 AT5G21130
fragaria_vesca FvH4_3g27690
malus_domestica MD00G1106600.v1.1 MD00G1119400.v1.1
prunus_persica Prupe.6G155500_v2.0.a1
pyrus_communis pycom11g15880 pycom5153684g00370
rosa_chinensis RchiOBHm_Chr5g0049981
rosa_laevigata RLG00000034674
rosa_multiflora Rmu_co8414557.1_g000001
rosa_roxburghii Rroxscaffold_1G00031180
rosa_rugosa Rorug05G0256200
rosa_samantha Rh5BG340000 Rh5CG366000 Rh5DG352600
rosa_wichuraiana Rw5G031100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccBSI CCGCTC 2 cut(s) 448, 564
AccI GTMKAC 2 cut(s) 135, 374
AccII CGCG 3 cut(s) 213, 362, 584
AclWI GGATC 6 cut(s) 223, 236, 443, 575, 773, 786
AcoI YGGCCR 2 cut(s) 3, 459
AcyI GRCGYC 2 cut(s) 47, 741
AfiI CCNNNNNNNGG 4 cut(s) 97, 98, 151, 622
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 3 cut(s) 340, 349, 544
AjiI CACGTC 2 cut(s) 397, 684
AluBI AGCT 2 cut(s) 456, 730
AluI AGCT 2 cut(s) 456, 730
AlwI GGATC 6 cut(s) 223, 236, 443, 575, 773, 786
Ama87I CYCGRG 2 cut(s) 19, 302
AoxI GGCC 2 cut(s) 3, 459
ApeKI GCWGC 2 cut(s) 35, 207
AspLEI GCGC 4 cut(s) 49, 500, 584, 610
AspS9I GGNCC 4 cut(s) 102, 112, 127, 672
AsuC2I CCSGG 3 cut(s) 20, 21, 99
AsuHPI GGTGA 2 cut(s) 346, 400
AvaI CYCGRG 2 cut(s) 19, 302
AvaII GGWCC 4 cut(s) 102, 112, 127, 672
BamHI GGATCC 2 cut(s) 228, 778
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 274
BbvCI CCTCAGC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 22, 194
BccI CCATC 2 cut(s) 275, 386
BceAI ACGGC 4 cut(s) 34, 60, 263, 511
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BcnI CCSGG 3 cut(s) 20, 21, 99
BfoI RGCGCY 2 cut(s) 50, 501
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 8 cut(s) 36, 199, 208, 211, 273, 276, 360, 521
BlsI GCNGC 8 cut(s) 37, 200, 209, 212, 274, 277, 361, 522
Bme1390I CCNGG 3 cut(s) 20, 21, 99
Bme18I GGWCC 4 cut(s) 102, 112, 127, 672
BmeT110I CYCGRG 2 cut(s) 19, 302
BmgBI CACGTC 2 cut(s) 397, 684
BmgT120I GGNCC 4 cut(s) 102, 112, 127, 672
BmiI GGNNCC 5 cut(s) 48, 103, 230, 673, 780
BmrFI CCNGG 3 cut(s) 20, 21, 99
BmsI GCATC 2 cut(s) 150, 246
BpiI GAAGAC 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 93
BpuMI CCSGG 3 cut(s) 20, 21, 99
BsaAI YACGTR 1 cut(s) 109
BsaHI GRCGYC 2 cut(s) 47, 741
BsaJI CCNNGG 6 cut(s) 19, 98, 123, 232, 616, 782
BsaWI WCCGGW 1 cut(s) 483
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 98, 151, 622
Bse118I RCCGGY 3 cut(s) 31, 385, 505
BseDI CCNNGG 6 cut(s) 19, 98, 123, 232, 616, 782
BseGI GGATG 4 cut(s) 141, 237, 252, 480
BseLI CCNNNNNNNGG 4 cut(s) 97, 98, 151, 622
BseMII CTCAG 2 cut(s) 107, 750
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 22, 194
Bsh1236I CGCG 3 cut(s) 213, 362, 584
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 2 cut(s) 5, 461
BshNI GGYRCC 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 10
BsiHKCI CYCGRG 2 cut(s) 19, 302
BsiSI CCGG 7 cut(s) 20, 32, 98, 152, 386, 484, 506
BslFI GGGAC 3 cut(s) 115, 292, 464
BslI CCNNNNNNNGG 4 cut(s) 97, 98, 151, 622
BsmFI GGGAC 3 cut(s) 115, 292, 464
BsnI GGCC 2 cut(s) 5, 461
BsoBI CYCGRG 2 cut(s) 19, 302
Bsp1286I GDGCHC 1 cut(s) 652
Bsp143I GATC 6 cut(s) 228, 390, 435, 567, 690, 778
BspANI GGCC 2 cut(s) 5, 461
BspCNI CTCAG 2 cut(s) 106, 749
BspFNI CGCG 3 cut(s) 213, 362, 584
BspLI GGNNCC 5 cut(s) 48, 103, 230, 673, 780
BspPI GGATC 6 cut(s) 223, 236, 443, 575, 773, 786
BspT107I GGYRCC 1 cut(s) 46
BsrBI CCGCTC 2 cut(s) 448, 564
BsrFI RCCGGY 3 cut(s) 31, 385, 505
BssAI RCCGGY 3 cut(s) 31, 385, 505
BssECI CCNNGG 6 cut(s) 19, 98, 123, 232, 616, 782
BssMI GATC 6 cut(s) 228, 390, 435, 567, 690, 778
BssNI GRCGYC 2 cut(s) 47, 741
BssT1I CCWWGG 3 cut(s) 123, 616, 782
Bst4CI ACNGT 6 cut(s) 403, 550, 697, 710, 721, 773
BstACI GRCGYC 2 cut(s) 47, 741
BstAPI GCANNNNNTGC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 109
BstC8I GCNNGC 4 cut(s) 33, 57, 408, 507
BstDEI CTNAG 2 cut(s) 93, 736
BstF5I GGATG 4 cut(s) 141, 237, 252, 480
BstFNI CGCG 3 cut(s) 213, 362, 584
BstH2I RGCGCY 2 cut(s) 50, 501
BstHHI GCGC 4 cut(s) 49, 500, 584, 610
BstKTI GATC 6 cut(s) 231, 393, 438, 570, 693, 781
BstMBI GATC 6 cut(s) 228, 390, 435, 567, 690, 778
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 6 cut(s) 44, 204, 207, 216, 272, 506
BstNSI RCATGY 2 cut(s) 59, 644
BstSCI CCNGG 3 cut(s) 18, 19, 97
BstUI CGCG 3 cut(s) 213, 362, 584
BstV1I GCAGC 2 cut(s) 22, 194
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 3 cut(s) 228, 435, 778
BstYI RGATCY 3 cut(s) 228, 435, 778
BsuRI GGCC 2 cut(s) 5, 461
BtgZI GCGATG 1 cut(s) 253
BtrI CACGTC 2 cut(s) 397, 684
BtsCI GGATG 4 cut(s) 141, 237, 252, 480
BtsIMutI CAGTG 2 cut(s) 474, 693
Cac8I GCNNGC 4 cut(s) 33, 57, 408, 507
CfoI GCGC 4 cut(s) 49, 500, 584, 610
Cfr10I RCCGGY 3 cut(s) 31, 385, 505
Cfr13I GGNCC 4 cut(s) 102, 112, 127, 672
Cfr9I CCCGGG 1 cut(s) 19
CseI GACGC 3 cut(s) 202, 351, 749
CviAII CATG 2 cut(s) 56, 641
DdeI CTNAG 2 cut(s) 93, 736
DinI GGCGCC 1 cut(s) 48
DpnI GATC 6 cut(s) 230, 392, 437, 569, 692, 780
DpnII GATC 6 cut(s) 228, 390, 435, 567, 690, 778
EaeI YGGCCR 2 cut(s) 3, 459
EciI GGCGGA 2 cut(s) 253, 287
Eco130I CCWWGG 3 cut(s) 123, 616, 782
Eco24I GRGCYC 1 cut(s) 652
Eco47I GGWCC 4 cut(s) 102, 112, 127, 672
Eco88I CYCGRG 2 cut(s) 19, 302
EcoO109I RGGNCCY 1 cut(s) 102
EcoT14I CCWWGG 3 cut(s) 123, 616, 782
EcoT38I GRGCYC 1 cut(s) 652
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 123, 616, 782
FaeI CATG 2 cut(s) 59, 644
FaiI YATR 2 cut(s) 57, 642
FaqI GGGAC 3 cut(s) 115, 292, 464
FatI CATG 2 cut(s) 55, 640
FauI CCCGC 3 cut(s) 191, 441, 557
FblI GTMKAC 2 cut(s) 135, 374
Fnu4HI GCNGC 8 cut(s) 36, 199, 208, 211, 273, 276, 360, 521
FokI GGATG 4 cut(s) 128, 224, 239, 467
FriOI GRGCYC 1 cut(s) 652
Fsp4HI GCNGC 8 cut(s) 36, 199, 208, 211, 273, 276, 360, 521
GlaI GCGC 4 cut(s) 48, 499, 583, 609
GluI GCNGC 8 cut(s) 36, 199, 208, 211, 273, 276, 360, 521
HaeII RGCGCY 2 cut(s) 50, 501
HaeIII GGCC 2 cut(s) 5, 461
HapII CCGG 7 cut(s) 20, 32, 98, 152, 386, 484, 506
HgaI GACGC 3 cut(s) 202, 351, 749
HhaI GCGC 4 cut(s) 49, 500, 584, 610
Hin1I GRCGYC 2 cut(s) 47, 741
Hin1II CATG 2 cut(s) 59, 644
Hin6I GCGC 4 cut(s) 47, 498, 582, 608
HinP1I GCGC 4 cut(s) 47, 498, 582, 608
HincII GTYRAC 2 cut(s) 375, 706
HindII GTYRAC 2 cut(s) 375, 706
HinfI GANTC 4 cut(s) 12, 26, 305, 660
HpaII CCGG 7 cut(s) 20, 32, 98, 152, 386, 484, 506
HphI GGTGA 2 cut(s) 346, 400
Hpy166II GTNNAC 5 cut(s) 16, 136, 375, 687, 706
Hpy188I TCNGA 2 cut(s) 647, 739
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 5 cut(s) 16, 136, 375, 687, 706
Hpy99I CGWCG 6 cut(s) 296, 367, 370, 373, 376, 398
HpyCH4III ACNGT 6 cut(s) 403, 550, 697, 710, 721, 773
HpyCH4IV ACGT 6 cut(s) 108, 351, 396, 465, 531, 683
HpyCH4V TGCA 4 cut(s) 55, 589, 640, 765
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 204, 207, 216, 272, 506
HpyF3I CTNAG 2 cut(s) 93, 736
HpySE526I ACGT 6 cut(s) 108, 351, 396, 465, 531, 683
Hsp92I GRCGYC 2 cut(s) 47, 741
Hsp92II CATG 2 cut(s) 59, 644
HspAI GCGC 4 cut(s) 47, 498, 582, 608
KasI GGCGCC 1 cut(s) 46
KroI GCCGGC 2 cut(s) 31, 505
KroNI GCCGGC 2 cut(s) 33, 507
Kzo9I GATC 6 cut(s) 228, 390, 435, 567, 690, 778
LmnI GCTCC 1 cut(s) 561
Lsp1109I GCAGC 2 cut(s) 22, 194
LweI GCATC 2 cut(s) 150, 246
MaeII ACGT 6 cut(s) 108, 351, 396, 465, 531, 683
MaeIII GTNAC 5 cut(s) 352, 397, 491, 532, 630
MalI GATC 6 cut(s) 230, 392, 437, 569, 692, 780
MbiI CCGCTC 2 cut(s) 448, 564
MboI GATC 6 cut(s) 228, 390, 435, 567, 690, 778
MboII GAAGA 2 cut(s) 274, 538
MflI RGATCY 3 cut(s) 228, 435, 778
MhlI GDGCHC 1 cut(s) 652
Mly113I GGCGCC 1 cut(s) 47
MlyI GAGTC 2 cut(s) 21, 314
MmeI TCCRAC 1 cut(s) 87
MroNI GCCGGC 2 cut(s) 31, 505
MseI TTAA 3 cut(s) 678, 699, 716
MspI CCGG 7 cut(s) 20, 32, 98, 152, 386, 484, 506
MspR9I CCNGG 3 cut(s) 20, 21, 99
MvnI CGCG 3 cut(s) 213, 362, 584
MwoI GCNNNNNNNGC 6 cut(s) 44, 204, 207, 216, 272, 506
NaeI GCCGGC 2 cut(s) 33, 507
NarI GGCGCC 1 cut(s) 47
NciI CCSGG 3 cut(s) 20, 21, 99
NdeII GATC 6 cut(s) 228, 390, 435, 567, 690, 778
NgoMIV GCCGGC 2 cut(s) 31, 505
NlaIII CATG 2 cut(s) 59, 644
NlaIV GGNNCC 5 cut(s) 48, 103, 230, 673, 780
NmeAIII GCCGAG 2 cut(s) 213, 437
NmuCI GTSAC 4 cut(s) 352, 397, 491, 532
NspI RCATGY 2 cut(s) 59, 644
PaeI GCATGC 1 cut(s) 59
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 300, 371, 399
PdiI GCCGGC 2 cut(s) 33, 507
PfeI GAWTC 2 cut(s) 26, 660
PflFI GACNNNGTC 2 cut(s) 11, 131
PkrI GCNGC 8 cut(s) 37, 200, 209, 212, 274, 277, 361, 522
PleI GAGTC 2 cut(s) 20, 313
PluTI GGCGCC 1 cut(s) 50
PpsI GAGTC 2 cut(s) 20, 313
Ppu21I YACGTR 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 48, 103, 230, 673, 780
PspPI GGNCC 4 cut(s) 102, 112, 127, 672
PspPPI RGGWCCY 1 cut(s) 102
PsuI RGATCY 3 cut(s) 228, 435, 778
PsyI GACNNNGTC 2 cut(s) 11, 131
SalI GTCGAC 1 cut(s) 373
SaqAI TTAA 3 cut(s) 678, 699, 716
SatI GCNGC 8 cut(s) 36, 199, 208, 211, 273, 276, 360, 521
Sau3AI GATC 6 cut(s) 228, 390, 435, 567, 690, 778
Sau96I GGNCC 4 cut(s) 102, 112, 127, 672
SchI GAGTC 2 cut(s) 21, 314
ScrFI CCNGG 3 cut(s) 20, 21, 99
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 2 cut(s) 150, 246
SfoI GGCGCC 1 cut(s) 48
SgrAI CRCCGGYG 1 cut(s) 385
SinI GGWCC 4 cut(s) 102, 112, 127, 672
SmaI CCCGGG 1 cut(s) 21
SphI GCATGC 1 cut(s) 59
SspDI GGCGCC 1 cut(s) 46
StyD4I CCNGG 3 cut(s) 18, 19, 97
StyI CCWWGG 3 cut(s) 123, 616, 782
TaaI ACNGT 6 cut(s) 403, 550, 697, 710, 721, 773
TaiI ACGT 6 cut(s) 111, 354, 399, 468, 534, 686
TaqI TCGA 2 cut(s) 374, 393
TaqII GACCGA 1 cut(s) 24
TauI GCSGC 6 cut(s) 201, 213, 275, 278, 362, 523
TfiI GAWTC 2 cut(s) 26, 660
Tru1I TTAA 3 cut(s) 678, 699, 716
Tru9I TTAA 3 cut(s) 678, 699, 716
TscAI CASTG 2 cut(s) 481, 700
TseFI GTSAC 4 cut(s) 352, 397, 491, 532
TseI GCWGC 2 cut(s) 35, 207
Tsp45I GTSAC 4 cut(s) 352, 397, 491, 532
TspGWI ACGGA 2 cut(s) 310, 551
TspMI CCCGGG 1 cut(s) 19
TspRI CASTG 2 cut(s) 481, 700
Tth111I GACNNNGTC 2 cut(s) 11, 131
VpaK11BI GGWCC 4 cut(s) 102, 112, 127, 672
XceI RCATGY 2 cut(s) 59, 644
XmaI CCCGGG 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 135, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.