Rw5G031100

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013178
Physical Location & Seq
Forward (+)
50542668 .. 50544134
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031100.1

Sequence Viewer

Length: 780 bp
ATGGCCGACCGAGTCCACCCGGCTGCCCAAACGGCGCCGTTGCATGCCTCCAACGACAACGACAACAACAACAACAACTCTGGCCTCCCTCAGCCGGGGACCTACGTGGTCCAAATCCCCAAGGACCAAGTCTACCGCGTCCCACCGCCGGAGAACGCCACCCTCTACAAGAACTACACTCGCCGAAAGCCTAGCCGCAACTGCTGCCGCGTCTGCTTCTGCTGGATCCTCGGCATCCTTGCCTCTCTCATCCTCCTCTCCGCCATCGCCGCCGCAGTCTTCTACCTCGTCGTCCGCCCCGAGTCCCCCAACTACAACGTCCAAAAAATCGCATTCAAAGGCTTCAACTTCACCGCCGCGTCGTCGACCATCTCGCCAGTGATCGATGTGACAGTGCGTGCGGATAACCCTAACAGGAAAATCGGGATCTACTACGAGCGAGACAGCTCGGCCAACGTGTTCTACACTCACATCCAGTTGTGTGACGGCGCTCTGCCGGCGTTTTATCAGCCGCCGAAGAACGTGACGGAGTTGAAAACGGTGCTGACGGGGAGCGGGATCAGGCTGACGAGCGCGGTGCAAAACGGGCTGGTGGTGGCGCAGAGCCAAGGCAAGGTGGCGTTACTTCTGCATGTCCGAGCCCCCGTTAGAATCAAAGTCGGGTCCCTAAAGACGTGGACGATTACCGTTAAAGTTGACTGTGATTTAACGGTGGATAAGCTGACCTCAGACGCCAAGATTGTTTCTAAAGACTGCAACTACGGTGTGGATCCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0030054 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

259

Amino Acids

28.45

Weight (kDa)

9.3

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 131 - 234 2.4e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27080 AT2G27080 AT5G21130
fragaria_vesca FvH4_3g27690
malus_domestica MD00G1106600.v1.1 MD00G1119400.v1.1
prunus_persica Prupe.6G155500_v2.0.a1
pyrus_communis pycom11g15880 pycom5153684g00370
rosa_chinensis RchiOBHm_Chr5g0049981
rosa_laevigata RLG00000034674
rosa_multiflora Rmu_co8414557.1_g000001
rosa_roxburghii Rroxscaffold_1G00031180
rosa_rugosa Rorug05G0256200
rosa_samantha Rh5BG340000 Rh5CG366000 Rh5DG352600
rosa_wichuraiana Rw5G031100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 555
AccI GTMKAC 2 cut(s) 132, 365
AccII CGCG 4 cut(s) 138, 210, 359, 575
AclWI GGATC 6 cut(s) 220, 233, 434, 566, 764, 777
AcoI YGGCCR 2 cut(s) 3, 450
AcyI GRCGYC 2 cut(s) 35, 732
AfiI CCNNNNNNNGG 4 cut(s) 94, 95, 148, 613
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 3 cut(s) 337, 346, 535
AjiI CACGTC 1 cut(s) 675
AluBI AGCT 2 cut(s) 447, 721
AluI AGCT 2 cut(s) 447, 721
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 6 cut(s) 220, 233, 434, 566, 764, 777
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 3, 82, 450
ApeKI GCWGC 2 cut(s) 23, 204
AspLEI GCGC 4 cut(s) 37, 491, 575, 601
AspS9I GGNCC 4 cut(s) 99, 109, 124, 663
AsuC2I CCSGG 2 cut(s) 20, 96
AsuHPI GGTGA 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 4 cut(s) 99, 109, 124, 663
BamHI GGATCC 2 cut(s) 225, 769
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 271
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 10, 191
BccI CCATC 2 cut(s) 272, 377
BceAI ACGGC 3 cut(s) 22, 48, 502
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BcnI CCSGG 2 cut(s) 20, 96
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 1 cut(s) 192
BfoI RGCGCY 2 cut(s) 38, 492
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 8 cut(s) 24, 196, 205, 208, 270, 273, 357, 512
BlsI GCNGC 8 cut(s) 25, 197, 206, 209, 271, 274, 358, 513
Bme1390I CCNGG 2 cut(s) 20, 96
Bme18I GGWCC 4 cut(s) 99, 109, 124, 663
BmeT110I CYCGRG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 675
BmgT120I GGNCC 4 cut(s) 99, 109, 124, 663
BmiI GGNNCC 6 cut(s) 36, 100, 227, 664, 665, 771
BmrFI CCNGG 2 cut(s) 20, 96
BmsI GCATC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 271
Bpu10I CCTNAGC 1 cut(s) 90
BpuMI CCSGG 2 cut(s) 20, 96
Bsa29I ATCGAT 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 2 cut(s) 35, 732
BsaJI CCNNGG 5 cut(s) 95, 120, 229, 607, 773
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 95, 148, 613
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 377, 475
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 5 cut(s) 95, 120, 229, 607, 773
BseGI GGATG 3 cut(s) 234, 249, 471
BseLI CCNNNNNNNGG 4 cut(s) 94, 95, 148, 613
BseMII CTCAG 2 cut(s) 104, 741
BseNI ACTGG 2 cut(s) 377, 475
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 2 cut(s) 10, 191
Bsh1236I CGCG 4 cut(s) 138, 210, 359, 575
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 5, 84, 452
BshNI GGYRCC 1 cut(s) 34
BshVI ATCGAT 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 20, 95, 149, 497
BslFI GGGAC 4 cut(s) 112, 125, 289, 649
BslI CCNNNNNNNGG 4 cut(s) 94, 95, 148, 613
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 4 cut(s) 112, 125, 289, 649
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 3 cut(s) 5, 84, 452
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 5 cut(s) 225, 381, 426, 558, 769
BspANI GGCC 3 cut(s) 5, 84, 452
BspCNI CTCAG 2 cut(s) 103, 740
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 4 cut(s) 138, 210, 359, 575
BspLI GGNNCC 6 cut(s) 36, 100, 227, 664, 665, 771
BspPI GGATC 6 cut(s) 220, 233, 434, 566, 764, 777
BspT107I GGYRCC 1 cut(s) 34
BsrBI CCGCTC 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 2 cut(s) 377, 475
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 5 cut(s) 95, 120, 229, 607, 773
BssMI GATC 5 cut(s) 225, 381, 426, 558, 769
BssNI GRCGYC 2 cut(s) 35, 732
BssT1I CCWWGG 3 cut(s) 120, 607, 773
Bst4CI ACNGT 6 cut(s) 394, 541, 688, 701, 712, 764
BstACI GRCGYC 2 cut(s) 35, 732
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 45, 399, 498
BstDEI CTNAG 2 cut(s) 90, 727
BstF5I GGATG 3 cut(s) 234, 249, 471
BstFNI CGCG 4 cut(s) 138, 210, 359, 575
BstH2I RGCGCY 2 cut(s) 38, 492
BstHHI GCGC 4 cut(s) 37, 491, 575, 601
BstKTI GATC 5 cut(s) 228, 384, 429, 561, 772
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 5 cut(s) 225, 381, 426, 558, 769
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 7 cut(s) 32, 201, 204, 213, 269, 497, 586
BstNSI RCATGY 2 cut(s) 47, 635
BstSCI CCNGG 2 cut(s) 18, 94
BstUI CGCG 4 cut(s) 138, 210, 359, 575
BstV1I GCAGC 2 cut(s) 10, 191
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 3 cut(s) 225, 426, 769
BstYI RGATCY 3 cut(s) 225, 426, 769
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 3 cut(s) 5, 84, 452
BsuTUI ATCGAT 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 675
BtsCI GGATG 3 cut(s) 234, 249, 471
BtsIMutI CAGTG 2 cut(s) 384, 399
Cac8I GCNNGC 3 cut(s) 45, 399, 498
CfoI GCGC 4 cut(s) 37, 491, 575, 601
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 4 cut(s) 99, 109, 124, 663
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 4 cut(s) 127, 199, 348, 740
CviAII CATG 2 cut(s) 44, 632
DdeI CTNAG 2 cut(s) 90, 727
DinI GGCGCC 1 cut(s) 36
DpnI GATC 5 cut(s) 227, 383, 428, 560, 771
DpnII GATC 5 cut(s) 225, 381, 426, 558, 769
EaeI YGGCCR 2 cut(s) 3, 450
EciI GGCGGA 2 cut(s) 250, 284
Eco130I CCWWGG 3 cut(s) 120, 607, 773
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 4 cut(s) 99, 109, 124, 663
Eco88I CYCGRG 1 cut(s) 299
EcoO109I RGGNCCY 2 cut(s) 99, 663
EcoT14I CCWWGG 3 cut(s) 120, 607, 773
EcoT38I GRGCYC 1 cut(s) 643
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 3 cut(s) 120, 607, 773
FaeI CATG 2 cut(s) 47, 635
FaiI YATR 2 cut(s) 45, 633
FaqI GGGAC 4 cut(s) 112, 125, 289, 649
FatI CATG 2 cut(s) 43, 631
FauI CCCGC 1 cut(s) 548
FblI GTMKAC 2 cut(s) 132, 365
Fnu4HI GCNGC 8 cut(s) 24, 196, 205, 208, 270, 273, 357, 512
FokI GGATG 3 cut(s) 221, 236, 458
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 8 cut(s) 24, 196, 205, 208, 270, 273, 357, 512
FspBI CTAG 1 cut(s) 192
GlaI GCGC 4 cut(s) 36, 490, 574, 600
GluI GCNGC 8 cut(s) 24, 196, 205, 208, 270, 273, 357, 512
HaeII RGCGCY 2 cut(s) 38, 492
HaeIII GGCC 3 cut(s) 5, 84, 452
HapII CCGG 4 cut(s) 20, 95, 149, 497
HgaI GACGC 4 cut(s) 127, 199, 348, 740
HhaI GCGC 4 cut(s) 37, 491, 575, 601
Hin1I GRCGYC 2 cut(s) 35, 732
Hin1II CATG 2 cut(s) 47, 635
Hin6I GCGC 4 cut(s) 35, 489, 573, 599
HinP1I GCGC 4 cut(s) 35, 489, 573, 599
HincII GTYRAC 2 cut(s) 366, 697
HindII GTYRAC 2 cut(s) 366, 697
HinfI GANTC 3 cut(s) 12, 302, 651
HpaII CCGG 4 cut(s) 20, 95, 149, 497
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 5 cut(s) 16, 133, 366, 678, 697
Hpy188I TCNGA 2 cut(s) 638, 730
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 5 cut(s) 16, 133, 366, 678, 697
Hpy99I CGWCG 3 cut(s) 293, 364, 367
HpyCH4III ACNGT 6 cut(s) 394, 541, 688, 701, 712, 764
HpyCH4IV ACGT 5 cut(s) 105, 318, 456, 522, 674
HpyCH4V TGCA 4 cut(s) 43, 580, 631, 756
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 201, 204, 213, 269, 497, 586
HpyF3I CTNAG 2 cut(s) 90, 727
HpySE526I ACGT 5 cut(s) 105, 318, 456, 522, 674
Hsp92I GRCGYC 2 cut(s) 35, 732
Hsp92II CATG 2 cut(s) 47, 635
HspAI GCGC 4 cut(s) 35, 489, 573, 599
KasI GGCGCC 1 cut(s) 34
KflI GGGWCCC 1 cut(s) 663
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Kzo9I GATC 5 cut(s) 225, 381, 426, 558, 769
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 2 cut(s) 10, 191
LweI GCATC 1 cut(s) 243
MaeI CTAG 1 cut(s) 192
MaeII ACGT 5 cut(s) 105, 318, 456, 522, 674
MaeIII GTNAC 4 cut(s) 388, 482, 523, 621
MalI GATC 5 cut(s) 227, 383, 428, 560, 771
MbiI CCGCTC 1 cut(s) 555
MboI GATC 5 cut(s) 225, 381, 426, 558, 769
MboII GAAGA 2 cut(s) 271, 529
MflI RGATCY 3 cut(s) 225, 426, 769
MhlI GDGCHC 1 cut(s) 643
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 2 cut(s) 21, 311
MmeI TCCRAC 1 cut(s) 75
MroNI GCCGGC 1 cut(s) 496
MseI TTAA 2 cut(s) 690, 707
MspI CCGG 4 cut(s) 20, 95, 149, 497
MspR9I CCNGG 2 cut(s) 20, 96
Mva1269I GAATGC 1 cut(s) 332
MvnI CGCG 4 cut(s) 138, 210, 359, 575
MwoI GCNNNNNNNGC 7 cut(s) 32, 201, 204, 213, 269, 497, 586
NaeI GCCGGC 1 cut(s) 498
NarI GGCGCC 1 cut(s) 35
NciI CCSGG 2 cut(s) 20, 96
NdeII GATC 5 cut(s) 225, 381, 426, 558, 769
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 2 cut(s) 47, 635
NlaIV GGNNCC 6 cut(s) 36, 100, 227, 664, 665, 771
NmeAIII GCCGAG 2 cut(s) 210, 428
NmuCI GTSAC 3 cut(s) 388, 482, 523
NspI RCATGY 2 cut(s) 47, 635
PaeI GCATGC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 332
PdiI GCCGGC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 651
PflFI GACNNNGTC 2 cut(s) 11, 128
PkrI GCNGC 8 cut(s) 25, 197, 206, 209, 271, 274, 358, 513
PleI GAGTC 2 cut(s) 20, 310
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 2 cut(s) 20, 310
Ppu21I YACGTR 1 cut(s) 106
PpuMI RGGWCCY 2 cut(s) 99, 663
Psp5II RGGWCCY 2 cut(s) 99, 663
PspN4I GGNNCC 6 cut(s) 36, 100, 227, 664, 665, 771
PspPI GGNCC 4 cut(s) 99, 109, 124, 663
PspPPI RGGWCCY 2 cut(s) 99, 663
PsuI RGATCY 3 cut(s) 225, 426, 769
PsyI GACNNNGTC 2 cut(s) 11, 128
SalI GTCGAC 1 cut(s) 364
SaqAI TTAA 2 cut(s) 690, 707
SatI GCNGC 8 cut(s) 24, 196, 205, 208, 270, 273, 357, 512
Sau3AI GATC 5 cut(s) 225, 381, 426, 558, 769
Sau96I GGNCC 4 cut(s) 99, 109, 124, 663
SchI GAGTC 2 cut(s) 21, 311
ScrFI CCNGG 2 cut(s) 20, 96
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 1 cut(s) 243
SfoI GGCGCC 1 cut(s) 36
SinI GGWCC 4 cut(s) 99, 109, 124, 663
SphI GCATGC 1 cut(s) 47
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 2 cut(s) 18, 94
StyI CCWWGG 3 cut(s) 120, 607, 773
TaaI ACNGT 6 cut(s) 394, 541, 688, 701, 712, 764
TaiI ACGT 5 cut(s) 108, 321, 459, 525, 677
TaqI TCGA 2 cut(s) 365, 384
TaqII GACCGA 1 cut(s) 24
TauI GCSGC 6 cut(s) 198, 210, 272, 275, 359, 514
TfiI GAWTC 1 cut(s) 651
Tru1I TTAA 2 cut(s) 690, 707
Tru9I TTAA 2 cut(s) 690, 707
TscAI CASTG 2 cut(s) 384, 399
TseFI GTSAC 3 cut(s) 388, 482, 523
TseI GCWGC 2 cut(s) 23, 204
Tsp45I GTSAC 3 cut(s) 388, 482, 523
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 2 cut(s) 384, 399
Tth111I GACNNNGTC 2 cut(s) 11, 128
VpaK11BI GGWCC 4 cut(s) 99, 109, 124, 663
XceI RCATGY 2 cut(s) 47, 635
XmiI GTMKAC 2 cut(s) 132, 365
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.