Rroxscaffold_1G00051750

ethanolamine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013742
Physical Location & Seq
Forward (+)
72471629 .. 72476025
4397 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00051750.1

Sequence Viewer

Length: 453 bp
ATGCCGGAGGATACCAGAATGGCCTGGAGAAAAGACTTCTCCTCCGCTCAGATCTGCAGTCTTCGTCCCACAGTCCCACTCGATCTCAGCTCGACCATGCTTTCGTCAGCCTTATTCTCCGCAAAGCCGTCTCCAAGCTCGGTGAGGCTCGCTGTCCGCGCATTTCTGATTCCGATTGAATTTGTTGCTATTGATTATAACAGGCCTCTTTCGGATCAAGGCCCTTTGGACATTGTGTTGCATAAGTTTGCTGCTTCCATTAGGAAATGGAGACTTACAGAGCCATACAAAGTACTGAAGGCTACTGTGAAAGAAGAGGATGGAAATGACGTGTCTGTAACAGTTAGATTGTATGGCCCCAACACTTATTACGTTGAAAACACAGAGCAGGCAATCAAGTACCTCTCAGCTGCAGGATTTGGTGCAAATTTGCTTGGAAATGGAATGGTGTAG

Protein Analysis

150

Amino Acids

16.61

Weight (kDa)

8.71

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ins134_P3_kin_N PF17927 58 - 90 6.3e-08 Inositol 1,3,4-trisphosphate 5/6-kinase pre-ATP-grasp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 198
AccBSI CCGCTC 1 cut(s) 47
AccII CGCG 1 cut(s) 159
AciI CCGC 3 cut(s) 45, 120, 157
AclWI GGATC 1 cut(s) 222
AcsI RAATTY 2 cut(s) 179, 427
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 2 cut(s) 294, 401
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 2 cut(s) 179, 377
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 3 cut(s) 90, 138, 410
AluI AGCT 3 cut(s) 90, 138, 410
Alw26I GTCTC 2 cut(s) 135, 265
AlwI GGATC 1 cut(s) 222
AoxI GGCC 4 cut(s) 21, 203, 220, 355
ApeKI GCWGC 2 cut(s) 251, 410
ApoI RAATTY 2 cut(s) 179, 427
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 221, 356
AsuHPI GGTGA 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 238, 397
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 25
BciVI GTATCC 1 cut(s) 4
BcoDI GTCTC 2 cut(s) 135, 265
BfmI CTRYAG 2 cut(s) 55, 411
BfuI GTATCC 1 cut(s) 4
BglII AGATCT 1 cut(s) 51
BisI GCNGC 2 cut(s) 252, 411
BlsI GCNGC 2 cut(s) 253, 412
BmcAI AGTACT 1 cut(s) 294
Bme1390I CCNGG 1 cut(s) 25
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 2 cut(s) 221, 356
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
BseBI CCWGG 1 cut(s) 25
BseGI GGATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 62, 100, 420
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 238, 397
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 4 cut(s) 23, 205, 222, 357
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 2 cut(s) 51, 59
BsmAI GTCTC 2 cut(s) 135, 265
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 51, 59
BsnI GGCC 4 cut(s) 23, 205, 222, 357
Bsp143I GATC 3 cut(s) 51, 82, 214
BspACI CCGC 3 cut(s) 45, 120, 157
BspANI GGCC 4 cut(s) 23, 205, 222, 357
BspCNI CTCAG 3 cut(s) 61, 99, 419
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 358
BspMAI CTGCAG 2 cut(s) 59, 415
BspPI GGATC 1 cut(s) 222
BsrBI CCGCTC 1 cut(s) 47
BssMI GATC 3 cut(s) 51, 82, 214
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 3 cut(s) 73, 307, 343
Bst6I CTCTTC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 150, 390
BstDEI CTNAG 3 cut(s) 48, 86, 406
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 54, 85, 217
BstMAI GTCTC 2 cut(s) 135, 265
BstMBI GATC 3 cut(s) 51, 82, 214
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 23
BstSFI CTRYAG 2 cut(s) 55, 411
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 238, 397
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 4 cut(s) 23, 205, 222, 357
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 150, 390
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 221, 356
Csp6I GTAC 2 cut(s) 293, 400
CviAII CATG 1 cut(s) 97
CviQI GTAC 2 cut(s) 293, 400
DdeI CTNAG 3 cut(s) 48, 86, 406
DpnI GATC 3 cut(s) 53, 84, 216
DpnII GATC 3 cut(s) 51, 82, 214
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 1 cut(s) 100
FaiI YATR 5 cut(s) 98, 198, 243, 286, 354
FaqI GGGAC 2 cut(s) 51, 59
FatI CATG 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 252, 411
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 2 cut(s) 252, 411
GlaI GCGC 1 cut(s) 160
GluI GCNGC 2 cut(s) 252, 411
GsuI CTGGAG 1 cut(s) 46
HaeIII GGCC 4 cut(s) 23, 205, 222, 357
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 161
Hin1II CATG 1 cut(s) 100
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 1 cut(s) 169
HpaII CCGG 1 cut(s) 5
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 4 cut(s) 51, 168, 174, 214
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 3 cut(s) 73, 307, 343
HpyCH4IV ACGT 2 cut(s) 330, 372
HpyCH4V TGCA 4 cut(s) 57, 241, 413, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 3 cut(s) 48, 86, 406
HpySE526I ACGT 2 cut(s) 330, 372
Hsp92II CATG 1 cut(s) 100
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 51, 82, 214
LpnPI CCDG 7 cut(s) 10, 18, 28, 37, 187, 374, 399
Lsp1109I GCAGC 2 cut(s) 238, 397
MaeII ACGT 2 cut(s) 330, 372
MaeIII GTNAC 1 cut(s) 337
MalI GATC 3 cut(s) 53, 84, 216
MbiI CCGCTC 1 cut(s) 47
MboI GATC 3 cut(s) 51, 82, 214
MboII GAAGA 2 cut(s) 53, 326
MflI RGATCY 1 cut(s) 51
MluCI AATT 2 cut(s) 179, 427
MnlI CCTC 5 cut(s) 52, 138, 216, 310, 413
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 3 cut(s) 51, 82, 214
NlaIII CATG 1 cut(s) 100
NlaIV GGNNCC 1 cut(s) 358
PceI AGGCCT 1 cut(s) 205
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 2 cut(s) 253, 412
PsiI TTATAA 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 2 cut(s) 221, 356
PstI CTGCAG 2 cut(s) 59, 415
PsuI RGATCY 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 2 cut(s) 294, 401
RsaNI GTAC 2 cut(s) 293, 400
SatI GCNGC 2 cut(s) 252, 411
Sau3AI GATC 3 cut(s) 51, 82, 214
Sau96I GGNCC 2 cut(s) 221, 356
ScaI AGTACT 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 25
SetI ASST 6 cut(s) 92, 140, 333, 375, 405, 412
SfcI CTRYAG 2 cut(s) 55, 411
Sse9I AATT 2 cut(s) 179, 427
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 3 cut(s) 45, 120, 157
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 73, 307, 343
TaiI ACGT 2 cut(s) 333, 375
TaqI TCGA 2 cut(s) 81, 92
TasI AATT 2 cut(s) 179, 427
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 1 cut(s) 169
TseI GCWGC 2 cut(s) 251, 410
XapI RAATTY 2 cut(s) 179, 427
ZrmI AGTACT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.