Rroxscaffold_1G00052110

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013726
Physical Location & Seq
Forward (+)
72820504 .. 72820934
431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052110.1

Sequence Viewer

Length: 282 bp
ATGAGTACAAGGAGGAGGGACTTGAAGAACAAGAGCCGACTTTTGCTTTTGCTGCAGATGAACCCGGTTCACAAGAAAGTGCCGGTGCTTATTCGCAATGGCAAAACCCTCTCCGAGTCGCTCATTGTGGTTCAATACATTGATGAGGTTTGGAAAGATGGGGCTCCATTGTTGCCTTCTGATCCTTACCTAAGATCCCAAGCCAAGTTCTGGGCGGACTTCGTCGACAAAAAGGATCAAAACTGCATTGATCTCATCAAAATACACGGACCAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

93

Amino Acids

10.75

Weight (kDa)

9.81

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 14 - 49 4.8e-08 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 210
AccI GTMKAC 1 cut(s) 225
AciI CCGC 1 cut(s) 215
AclWI GGATC 3 cut(s) 176, 189, 243
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 210
AgsI TTSAA 2 cut(s) 25, 134
AlwI GGATC 3 cut(s) 176, 189, 243
ApeKI GCWGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 39
BccI CCATC 1 cut(s) 152
BcnI CCSGG 1 cut(s) 65
BfmI CTRYAG 1 cut(s) 53
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse118I RCCGGY 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 39
BsiSI CCGG 2 cut(s) 65, 83
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 4 cut(s) 181, 194, 235, 250
BspACI CCGC 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 165
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 3 cut(s) 176, 189, 243
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 82
BssMI GATC 4 cut(s) 181, 194, 235, 250
BstDEI CTNAG 1 cut(s) 191
BstKTI GATC 4 cut(s) 184, 197, 238, 253
BstMBI GATC 4 cut(s) 181, 194, 235, 250
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 6
CviJI RGCY 3 cut(s) 36, 164, 203
CviKI_1 RGCY 3 cut(s) 36, 164, 203
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 191
DpnI GATC 4 cut(s) 183, 196, 237, 252
DpnII GATC 4 cut(s) 181, 194, 235, 250
EciI GGCGGA 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 166
FaiI YATR 1 cut(s) 280
FaqI GGGAC 1 cut(s) 32
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 53
GluI GCNGC 1 cut(s) 53
HapII CCGG 2 cut(s) 65, 83
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 65, 83
Hpy166II GTNNAC 2 cut(s) 70, 226
Hpy188I TCNGA 2 cut(s) 115, 181
Hpy8I GTNNAC 2 cut(s) 70, 226
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 55, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 1 cut(s) 191
Kzo9I GATC 4 cut(s) 181, 194, 235, 250
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 3 cut(s) 78, 96, 196
Lsp1109I GCAGC 1 cut(s) 39
MalI GATC 4 cut(s) 183, 196, 237, 252
MboI GATC 4 cut(s) 181, 194, 235, 250
MboII GAAGA 1 cut(s) 37
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 6, 9, 119, 139
MspI CCGG 2 cut(s) 65, 83
MspR9I CCNGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 52
NciI CCSGG 1 cut(s) 65
NdeII GATC 4 cut(s) 181, 194, 235, 250
NlaIV GGNNCC 1 cut(s) 165
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 210
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 194
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 4 cut(s) 181, 194, 235, 250
Sau96I GGNCC 1 cut(s) 269
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 1 cut(s) 166
SetI ASST 2 cut(s) 150, 192
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 269
SsiI CCGC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 63
TaqI TCGA 1 cut(s) 225
TatI WGTACW 1 cut(s) 5
TseI GCWGC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 282
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.