Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0015091

Physical Location & Seq

Genomic Coordinates Reverse (-)

8887069 .. 8889887

Gene Structure 2819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00086710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGCTGATTCCAAGCCTCTCGCTTCCCGCTCCCGCCAAATTCCCTCCGCCCCCTTCTTCTTCCTCTCTCCGACACCACCAAGACCCAAACTTTGAGCCTCTCCACCGCCGTCTGATCACCCAAATCAATGTCGGCCATCTCCGCAACGCAATCTCCACCCTCGATCTCATGGCCCTCCGCGGCTCCCACCCTGACCTCCCCACCTACTCCCTCCTCCTCAAGTCCTGCCTCCGCTCCCGCAACTTCCACCTCGGGAAGCTCGTCCACTCGCGGGTGACTCAGTCCCAACTCGACCCCGACTCTGTCATTCTCAACTCCTTGATTAGCCTCTACTCGAAATCGGGAGACTTTAAAAAGGCGCGATCGATCTTTCAAACTATGGGAGAGAAGAGAAATTTGGTGTCTTGGAGTGCCATGGTGTCGTGTTTCGCCAACAACGGCATGTCGTTGGAGGCGGTTTCGGCGTTTGTTGATATGCTTGAGGAGGGGTTTTACCCGAACGAGTTTTGCTTTGCGTCGGTTATTCGGGCGTGTTCGAATTCGGAGCATGTTGGGATTGGGAAGGTGGTTTTCGGGTCGGTGATTAAAACTGGGTATCTTGAGTCGGATGTGTGTGTTGGGTCTTCGTTGATTGATGTGTTTGCGAAGGGTGGTGGGGACTTAAGGGATGCATATAAGGTGTTTGAGAAAATGGCTGAGACGGATGTGGTGACTTGGAGCTTGATGATCACTAGGTTTGCGCAGATGGGTGATCCGAGAAAGGCAGTTGAGTTGTATATGGATATGTTGTCAAATGGGCTAATGCCGGACCAGTTTACAATGAGTGGTGTTGTGTCGGCTTGTTCAAAGTTGGGGTCGTTGGCGTTGGGGCAGCAGTTGCATTCGTGGGCTGTACGGTCTCGGTTGGGTTTGGATCACTGCGTTGGGTGTTGTTTGGTCGACATGTATGCCAAGTGTGGAGGTGGTGGGTCGATGAGTGATTCGAGGAAGGTGTTTGATCGGATGCGAGAGCATAGTGTTATGTCATGGACTGCTGTTATCACTGGATATGTGCAGGGTGGAGGGGGCAATGAGGAAGCTGTCAAGCTATTTGTTGAAATGATCTCGTGTGGTCATGTTTCGCCGAATCATTTCACGTTTGCTAGCATTCTGAAGGCTTGTGCAAACCTTTCCGATCTGCGTAAGGGAGAGCAAGTTCACTCCCTTGCAGTGAAACTTGGTCTTGCATCGGTTAATTGTGTGGGGAATTCACTTATTAGCATGTATGCAAAGTCTGGCCAGGTGGAAGATGCACGGAAAGCTTTTGATCTTCTGTATGAGAAGAATTTGATATCTTATAATACAATCGTTGATGCATATGCCAAGCATCTAGACACAGAAGAAGCCTTTGGTCTTCTCCATGAAGTCGAGAATACCGGACTTGGGGCTAGTGCTTTCACATTTGCTAGTCTCTTGAGTGGAGCTGCCAGCCTTTGTGCAGTTGATAAGGGCGAGCAAATACATTCCCGGATTATAAAATCAGGATTTGAGCCAAACCAAAGTATTTGCAATGCCTTGGTTTCTATGTATTCCAGGTGTGGAAACATAGACGCTGCTTTTCAAGTGTTTAATGAGATGGAAGATTGGAATGTTATATCATGGACTTCAATGATCACAGGTTTTGCAAAACATGGTTATGCGGCTAGAGCTGTGGGACTGTTTAACCAAATGCTTGAGGCTGGGTTAAAACCGAATGAGATCACATATATTGCTGTTTTATCAGCATGTAGCCATGCGGGTTTGATTTCTGAGGGGTGGAAACACTTGAAGGAAATGCACCAACAACATGGTATTGTCCCAAAAATGGAACACTATGCATGTATGGTTGATTTATTGGGCCGCTCAGGATCCCTTGTAGAAGCCATTGAGTTTATTAACTCAATGCCTTTTGAAGCTGATGCACTAATCTGGCGAACATTTCTTGGAGCATGCCGAGTGCATGGTGACATTGAGCTTGGGAAGCATGCGGCAAAGATGATTATTAAGCAGAACCCACATGATTCAGCGGCATATAGCTTACTATCAAATTTGTATGCTTCCACGGGTCAGTGGGAAGAAGTAGCAAATATAAGAAAAAATATGAAAGAGAAAGCTTTGGTCAAAGAAGCAGGTTCTAGCTGGATAGAGGTGAAGAATAAGATGCACAAGTTCCATGTTGGAGATACTTCGCACCCAAAAGCACGGGAGATTTATGATGAACTGGACCAATTGGGTTCAAAGATAAAGAAATTGGGTTACGTCCCCGATACAGATTTTGTACTTCATGAGGTGGAAGAGGAGCAAAAGGAATATTATTTGTTTCAGCACAGTGAGAAATTAGCAGTGGCATTTGGTCTTATAAGCACATCGAAATCGAAACCCATAAGAGTATTCAAGAATCTCCGTGTTTGTGGAGATTGTCACACTGCAATTAAGTATATTTCAAAAGCCACGGGAAGAGAAATAGTGGTAAGGGATTCAAATCGGTTTCATCAGTTCAAGGATGGGACATGTTCTTGCAATGACTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

850

Amino Acids

93.84

Weight (kDa)

7.03

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	132 - 180	9e-07	PPR repeat family
PPR_2	PF13041	235 - 283	4.7e-10	PPR repeat family
PPR	PF01535	237 - 267	9.7e-06	PPR repeat
PPR_2	PF13041	339 - 389	2.2e-07	PPR repeat family
PPR_2	PF13041	442 - 488	1.8e-06	PPR repeat family
PPR_2	PF13041	511 - 544	7e-07	PPR repeat family
PPR_2	PF13041	543 - 590	1.6e-11	PPR repeat family
PPR	PF01535	545 - 575	2e-06	PPR repeat
E_motif	PF20431	660 - 722	2.8e-16	E motif
Eplus_motif	PF20430	728 - 750	8.7e-07	E+ motif
DYW_deaminase	PF14432	758 - 850	1.4e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49170
fragaria_vesca	FvH4_6g45980 FvH4_6g45980
malus_domestica	MD09G1078500.v1.1 MD13G1104000.v1.1
prunus_persica	Prupe.3G245900_v2.0.a1
pyrus_communis	pycom09g00520
rosa_chinensis	RchiOBHm_Chr2g0164221
rosa_laevigata	RLG00000021445
rosa_multiflora	Rmu_sc0001427.1_g000014
rosa_roxburghii	Rroxscaffold_2G00086710
rosa_rugosa	Rorug02G0507100
rosa_samantha	Rh2BG586100 Rh2CG557000 Rh2DG595700
rosa_wichuraiana	Rw2G047630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1338, 1514, 2378
Acc16I	TGCGCA	1 cut(s)	741
Acc36I	ACCTGC	1 cut(s)	2138
AccBSI	CCGCTC	3 cut(s)	29, 234, 1881
AccI	GTMKAC	1 cut(s)	939
AccII	CGCG	3 cut(s)	180, 271, 361
AclWI	GGATC	4 cut(s)	746, 921, 1881, 1894
AcoI	YGGCCR	2 cut(s)	133, 1276
AcsI	RAATTY	6 cut(s)	38, 394, 538, 1246, 1324, 2065
AcuI	CTGAAG	1 cut(s)	1172
AfaI	GTAC	2 cut(s)	894, 2298
AfiI	CCNNNNNNNGG	3 cut(s)	271, 1422, 1843
AflII	CTTAAG	1 cut(s)	661
AflIII	ACRYGT	2 cut(s)	942, 2528
AjnI	CCWGG	2 cut(s)	1278, 1571
AjuI	GAANNNNNNNTTGG	4 cut(s)	380, 412, 1371, 1403
AloI	GAACNNNNNNTCC	2 cut(s)	1179, 1211
Alw26I	GTCTC	4 cut(s)	339, 692, 903, 1454
AlwI	GGATC	4 cut(s)	746, 921, 1881, 1894
Ama87I	CYCGRG	1 cut(s)	251
AoxI	GGCC	4 cut(s)	133, 171, 1276, 1876
ApeKI	GCWGC	3 cut(s)	871, 1463, 1592
ApoI	RAATTY	6 cut(s)	38, 394, 538, 1246, 1324, 2065
ArsI	GACNNNNNNTTYG	4 cut(s)	74, 106, 839, 871
Asp700I	GAANNNNTTC	1 cut(s)	1130
AspLEI	GCGC	2 cut(s)	361, 742
AspS9I	GGNCC	4 cut(s)	172, 808, 1876, 2242
AsuC2I	CCSGG	1 cut(s)	1507
AsuHPI	GGTGA	7 cut(s)	109, 286, 592, 721, 761, 1994, 2179
AsuII	TTCGAA	1 cut(s)	536
AsuNHI	GCTAGC	1 cut(s)	1142
AvaI	CYCGRG	1 cut(s)	251
AvaII	GGWCC	2 cut(s)	808, 2242
BalI	TGGCCA	1 cut(s)	1278
BamHI	GGATCC	1 cut(s)	1886
BauI	CACGAG	1 cut(s)	1105
BbsI	GAAGAC	2 cut(s)	615, 1385
BbvI	GCAGC	3 cut(s)	883, 1450, 1579
BccI	CCATC	4 cut(s)	144, 739, 1609, 2516
BceAI	ACGGC	2 cut(s)	93, 454
BcgI	CGANNNNNNTGC	2 cut(s)	929, 963
BciT130I	CCWGG	2 cut(s)	1280, 1573
BclI	TGATCA	3 cut(s)	114, 726, 1650
BcnI	CCSGG	1 cut(s)	1507
BcoDI	GTCTC	4 cut(s)	339, 692, 903, 1454
BfaI	CTAG	7 cut(s)	732, 1143, 1370, 1428, 1446, 1683, 2154
BfrI	CTTAAG	1 cut(s)	661
BfuAI	ACCTGC	1 cut(s)	2138
BisI	GCNGC	8 cut(s)	181, 872, 1464, 1593, 1680, 1879, 2007, 2046
BlsI	GCNGC	8 cut(s)	182, 873, 1465, 1594, 1681, 1880, 2008, 2047
Bme1390I	CCNGG	3 cut(s)	1280, 1507, 1573
Bme18I	GGWCC	2 cut(s)	808, 2242
BmeT110I	CYCGRG	1 cut(s)	251
BmgT120I	GGNCC	4 cut(s)	172, 808, 1876, 2242
BmiI	GGNNCC	2 cut(s)	184, 1888
BmrFI	CCNGG	3 cut(s)	1280, 1507, 1573
BmrI	ACTGGG	1 cut(s)	600
BmsI	GCATC	8 cut(s)	658, 993, 1235, 1279, 1342, 1375, 1927, 2169
BmtI	GCTAGC	1 cut(s)	1146
BmuI	ACTGGG	1 cut(s)	600
BpiI	GAAGAC	2 cut(s)	615, 1385
Bpu10I	CCTNAGC	1 cut(s)	1882
Bpu14I	TTCGAA	1 cut(s)	536
BpuEI	CTTGAG	5 cut(s)	203, 500, 620, 1474, 1733
BpuMI	CCSGG	1 cut(s)	1507
Bsa29I	ATCGAT	1 cut(s)	365
BsaBI	GATNNNNATC	1 cut(s)	2499
BsaI	GGTCTC	1 cut(s)	903
BsaJI	CCNNGG	6 cut(s)	178, 250, 414, 1554, 2079, 2469
BsaWI	WCCGGW	1 cut(s)	1415
BsaXI	ACNNNNNCTCC	4 cut(s)	137, 167, 1179, 1209
Bsc4I	CCNNNNNNNGG	3 cut(s)	271, 1422, 1843
Bse1I	ACTGG	5 cut(s)	595, 811, 1048, 2244, 2552
Bse3DI	GCAATG	3 cut(s)	1075, 1555, 2545
Bse8I	GATNNNNATC	1 cut(s)	2499
BseBI	CCWGG	2 cut(s)	1280, 1573
BseCI	ATCGAT	1 cut(s)	365
BseDI	CCNNGG	6 cut(s)	178, 250, 414, 1554, 2079, 2469
BseGI	GGATG	5 cut(s)	613, 673, 709, 1008, 2527
BseJI	GATNNNNATC	1 cut(s)	2499
BseLI	CCNNNNNNNGG	3 cut(s)	271, 1422, 1843
BseMI	GCAATG	3 cut(s)	1075, 1555, 2545
BseMII	CTCAG	4 cut(s)	293, 687, 1779, 1896
BseNI	ACTGG	5 cut(s)	595, 811, 1048, 2244, 2552
BseRI	GAGGAG	4 cut(s)	203, 206, 497, 2330
BseXI	GCAGC	3 cut(s)	883, 1450, 1579
BseYI	CCCAGC	1 cut(s)	1718
BsgI	GTGCAG	2 cut(s)	1073, 1497
Bsh1236I	CGCG	3 cut(s)	180, 271, 361
Bsh1285I	CGRYCG	1 cut(s)	365
BshFI	GGCC	4 cut(s)	135, 173, 1278, 1878
BshVI	ATCGAT	1 cut(s)	365
BsiEI	CGRYCG	1 cut(s)	365
BsiHKCI	CYCGRG	1 cut(s)	251
BsiSI	CCGG	3 cut(s)	806, 1416, 1507
BslFI	GGGAC	6 cut(s)	268, 671, 1707, 1820, 2264, 2539
BslI	CCNNNNNNNGG	3 cut(s)	271, 1422, 1843
BsmAI	GTCTC	4 cut(s)	339, 692, 903, 1454
BsmBI	CGTCTC	1 cut(s)	692
BsmFI	GGGAC	6 cut(s)	268, 671, 1707, 1820, 2264, 2539
BsmI	GAATGC	2 cut(s)	880, 1146
BsnI	GGCC	4 cut(s)	135, 173, 1278, 1878
Bso31I	GGTCTC	1 cut(s)	903
BsoBI	CYCGRG	1 cut(s)	251
Bsp119I	TTCGAA	1 cut(s)	536
Bsp19I	CCATGG	1 cut(s)	414
BspANI	GGCC	4 cut(s)	135, 173, 1278, 1878
BspCNI	CTCAG	4 cut(s)	292, 688, 1780, 1895
BspDI	ATCGAT	1 cut(s)	365
BspFNI	CGCG	3 cut(s)	180, 271, 361
BspHI	TCATGA	1 cut(s)	2302
BspLI	GGNNCC	2 cut(s)	184, 1888
BspMI	ACCTGC	1 cut(s)	2138
BspOI	GCTAGC	1 cut(s)	1146
BspPI	GGATC	4 cut(s)	746, 921, 1881, 1894
BspT104I	TTCGAA	1 cut(s)	536
BspTI	CTTAAG	1 cut(s)	661
BspTNI	GGTCTC	1 cut(s)	903
BsrBI	CCGCTC	3 cut(s)	29, 234, 1881
BsrDI	GCAATG	3 cut(s)	1075, 1555, 2545
BsrI	ACTGG	5 cut(s)	595, 811, 1048, 2244, 2552
BssECI	CCNNGG	6 cut(s)	178, 250, 414, 1554, 2079, 2469
BssSI	CACGAG	1 cut(s)	1105
BssT1I	CCWWGG	2 cut(s)	414, 1554
Bst2BI	CACGAG	1 cut(s)	1105
Bst2UI	CCWGG	2 cut(s)	1280, 1573
Bst4CI	ACNGT	3 cut(s)	897, 1698, 2348
Bst6I	CTCTTC	3 cut(s)	383, 2307, 2470
BstAFI	CTTAAG	1 cut(s)	661
BstAPI	GCANNNNNTGC	1 cut(s)	877
BstBI	TTCGAA	1 cut(s)	536
BstC8I	GCNNGC	5 cut(s)	1144, 1468, 1493, 1969, 2004
BstDEI	CTNAG	4 cut(s)	279, 696, 1788, 1882
BstDSI	CCRYGG	4 cut(s)	178, 414, 2079, 2469
BstF5I	GGATG	5 cut(s)	613, 673, 709, 1008, 2527
BstFNI	CGCG	3 cut(s)	180, 271, 361
BstHHI	GCGC	2 cut(s)	361, 742
BstMAI	GTCTC	4 cut(s)	339, 692, 903, 1454
BstMCI	CGRYCG	1 cut(s)	365
BstMWI	GCNNNNNNNGC	6 cut(s)	141, 461, 877, 1298, 1685, 1999
BstNI	CCWGG	2 cut(s)	1280, 1573
BstNSI	RCATGY	9 cut(s)	445, 551, 946, 1264, 1767, 1860, 1971, 2006, 2532
BstSCI	CCNGG	3 cut(s)	1278, 1505, 1571
BstUI	CGCG	3 cut(s)	180, 271, 361
BstV1I	GCAGC	3 cut(s)	883, 1450, 1579
BstV2I	GAAGAC	2 cut(s)	615, 1385
BstX2I	RGATCY	1 cut(s)	1886
BstXI	CCANNNNNNTGG	1 cut(s)	1826
BstYI	RGATCY	1 cut(s)	1886
Bsu15I	ATCGAT	1 cut(s)	365
BsuRI	GGCC	4 cut(s)	135, 173, 1278, 1878
BsuTUI	ATCGAT	1 cut(s)	365
BtgI	CCRYGG	4 cut(s)	178, 414, 2079, 2469
BtsCI	GGATG	5 cut(s)	613, 673, 709, 1008, 2527
BtsI	GCAGTG	4 cut(s)	916, 1215, 2367, 2442
BtsIMutI	CAGTG	7 cut(s)	916, 1041, 1215, 2093, 2353, 2367, 2442
BveI	ACCTGC	1 cut(s)	2138
Cac8I	GCNNGC	5 cut(s)	1144, 1468, 1493, 1969, 2004
CciI	TCATGA	1 cut(s)	2302
CfoI	GCGC	2 cut(s)	361, 742
Cfr13I	GGNCC	4 cut(s)	172, 808, 1876, 2242
Cfr42I	CCGCGG	1 cut(s)	181
ClaI	ATCGAT	1 cut(s)	365
CseI	GACGC	2 cut(s)	504, 1598
Csp6I	GTAC	2 cut(s)	893, 2297
CviQI	GTAC	2 cut(s)	893, 2297
DdeI	CTNAG	4 cut(s)	279, 696, 1788, 1882
DraI	TTTAAA	1 cut(s)	352
EaeI	YGGCCR	2 cut(s)	133, 1276
Eam1104I	CTCTTC	3 cut(s)	383, 2307, 2470
EarI	CTCTTC	3 cut(s)	383, 2307, 2470
EciI	GGCGGA	1 cut(s)	36
Eco130I	CCWWGG	2 cut(s)	414, 1554
Eco31I	GGTCTC	1 cut(s)	903
Eco32I	GATATC	1 cut(s)	1332
Eco47I	GGWCC	2 cut(s)	808, 2242
Eco57I	CTGAAG	1 cut(s)	1172
Eco88I	CYCGRG	1 cut(s)	251
EcoRI	GAATTC	2 cut(s)	538, 1246
EcoRII	CCWGG	2 cut(s)	1278, 1571
EcoRV	GATATC	1 cut(s)	1332
EcoT14I	CCWWGG	2 cut(s)	414, 1554
EcoT22I	ATGCAT	3 cut(s)	673, 1357, 1858
ErhI	CCWWGG	2 cut(s)	414, 1554
Esp3I	CGTCTC	1 cut(s)	692
FaqI	GGGAC	6 cut(s)	268, 671, 1707, 1820, 2264, 2539
FauI	CCCGC	5 cut(s)	34, 40, 245, 264, 1768
FauNDI	CATATG	1 cut(s)	1357
FbaI	TGATCA	3 cut(s)	114, 726, 1650
FblI	GTMKAC	1 cut(s)	939
Fnu4HI	GCNGC	8 cut(s)	181, 872, 1464, 1593, 1680, 1879, 2007, 2046
FokI	GGATG	5 cut(s)	620, 680, 716, 1015, 2534
Fsp4HI	GCNGC	8 cut(s)	181, 872, 1464, 1593, 1680, 1879, 2007, 2046
FspBI	CTAG	7 cut(s)	732, 1143, 1370, 1428, 1446, 1683, 2154
FspI	TGCGCA	1 cut(s)	741
GlaI	GCGC	2 cut(s)	360, 741
GluI	GCNGC	8 cut(s)	181, 872, 1464, 1593, 1680, 1879, 2007, 2046
GsaI	CCCAGC	1 cut(s)	1722
HaeIII	GGCC	4 cut(s)	135, 173, 1278, 1878
HapII	CCGG	3 cut(s)	806, 1416, 1507
HgaI	GACGC	2 cut(s)	504, 1598
HhaI	GCGC	2 cut(s)	361, 742
Hin6I	GCGC	2 cut(s)	359, 740
HinP1I	GCGC	2 cut(s)	359, 740
HincII	GTYRAC	1 cut(s)	940
HindII	GTYRAC	1 cut(s)	940
HindIII	AAGCTT	2 cut(s)	1299, 2130
HinfI	GANTC	9 cut(s)	7, 277, 299, 602, 980, 1126, 2039, 2416, 2495
HpaII	CCGG	3 cut(s)	806, 1416, 1507
HphI	GGTGA	7 cut(s)	109, 286, 592, 721, 761, 1994, 2179
Hpy166II	GTNNAC	4 cut(s)	265, 816, 940, 1198
Hpy188I	TCNGA	9 cut(s)	71, 114, 544, 607, 756, 1002, 1152, 1174, 1789
Hpy8I	GTNNAC	4 cut(s)	265, 816, 940, 1198
Hpy99I	CGWCG	1 cut(s)	520
HpyAV	CCTTC	6 cut(s)	63, 556, 640, 982, 1147, 1801
HpyCH4III	ACNGT	3 cut(s)	897, 1698, 2348
HpyCH4IV	ACGT	2 cut(s)	1136, 2277
HpyF10VI	GCNNNNNNNGC	6 cut(s)	141, 461, 877, 1298, 1685, 1999
HpyF3I	CTNAG	4 cut(s)	279, 696, 1788, 1882
HpySE526I	ACGT	2 cut(s)	1136, 2277
HspAI	GCGC	2 cut(s)	359, 740
Ksp22I	TGATCA	3 cut(s)	114, 726, 1650
KspI	CCGCGG	1 cut(s)	181
LmnI	GCTCC	8 cut(s)	34, 188, 239, 544, 717, 1460, 1964, 2317
Lsp1109I	GCAGC	3 cut(s)	883, 1450, 1579
LweI	GCATC	8 cut(s)	658, 993, 1235, 1279, 1342, 1375, 1927, 2169
MaeI	CTAG	7 cut(s)	732, 1143, 1370, 1428, 1446, 1683, 2154
MaeII	ACGT	2 cut(s)	1136, 2277
MaeIII	GTNAC	5 cut(s)	274, 709, 1982, 2273, 2438
MbiI	CCGCTC	3 cut(s)	29, 234, 1881
MfeI	CAATTG	1 cut(s)	2246
MflI	RGATCY	1 cut(s)	1886
MlsI	TGGCCA	1 cut(s)	1278
MluNI	TGGCCA	1 cut(s)	1278
MlyI	GAGTC	3 cut(s)	271, 293, 611
MmeI	TCCRAC	4 cut(s)	94, 429, 585, 2176
Mox20I	TGGCCA	1 cut(s)	1278
Mph1103I	ATGCAT	3 cut(s)	673, 1357, 1858
MroXI	GAANNNNTTC	1 cut(s)	1130
MscI	TGGCCA	1 cut(s)	1278
MslI	CAYNNNNRTG	1 cut(s)	1674
Msp20I	TGGCCA	1 cut(s)	1278
MspA1I	CMGCKG	2 cut(s)	180, 2045
MspCI	CTTAAG	1 cut(s)	661
MspI	CCGG	3 cut(s)	806, 1416, 1507
MspR9I	CCNGG	3 cut(s)	1280, 1507, 1573
MunI	CAATTG	1 cut(s)	2246
Mva1269I	GAATGC	2 cut(s)	880, 1146
MvaI	CCWGG	2 cut(s)	1280, 1573
MvnI	CGCG	3 cut(s)	180, 271, 361
MwoI	GCNNNNNNNGC	6 cut(s)	141, 461, 877, 1298, 1685, 1999
NciI	CCSGG	1 cut(s)	1507
NcoI	CCATGG	1 cut(s)	414
NdeI	CATATG	1 cut(s)	1357
NheI	GCTAGC	1 cut(s)	1142
NlaIV	GGNNCC	2 cut(s)	184, 1888
NmeAIII	GCCGAG	1 cut(s)	1997
NmuCI	GTSAC	4 cut(s)	274, 709, 1982, 2438
NsbI	TGCGCA	1 cut(s)	741
NsiI	ATGCAT	3 cut(s)	673, 1357, 1858
NspI	RCATGY	9 cut(s)	445, 551, 946, 1264, 1767, 1860, 1971, 2006, 2532
NspV	TTCGAA	1 cut(s)	536
PaeI	GCATGC	2 cut(s)	1971, 2006
PagI	TCATGA	1 cut(s)	2302
PciI	ACATGT	2 cut(s)	942, 2528
PcsI	WCGNNNNNNNCGW	1 cut(s)	258
PctI	GAATGC	2 cut(s)	880, 1146
PdmI	GAANNNNTTC	1 cut(s)	1130
PfeI	GAWTC	6 cut(s)	7, 980, 1126, 2039, 2416, 2495
PflFI	GACNNNGTC	2 cut(s)	280, 302
PfoI	TCCNGGA	1 cut(s)	1505
PkrI	GCNGC	8 cut(s)	182, 873, 1465, 1594, 1681, 1880, 2008, 2047
Ple19I	CGATCG	1 cut(s)	365
PleI	GAGTC	3 cut(s)	271, 293, 610
PpsI	GAGTC	3 cut(s)	271, 293, 610
PscI	ACATGT	2 cut(s)	942, 2528
PsiI	TTATAA	3 cut(s)	1338, 1514, 2378
Psp6I	CCWGG	2 cut(s)	1278, 1571
PspFI	CCCAGC	1 cut(s)	1718
PspGI	CCWGG	2 cut(s)	1278, 1571
PspN4I	GGNNCC	2 cut(s)	184, 1888
PspPI	GGNCC	4 cut(s)	172, 808, 1876, 2242
PsuI	RGATCY	1 cut(s)	1886
PsyI	GACNNNGTC	2 cut(s)	280, 302
PvuI	CGATCG	1 cut(s)	365
RsaI	GTAC	2 cut(s)	894, 2298
RsaNI	GTAC	2 cut(s)	893, 2297
RseI	CAYNNNNRTG	1 cut(s)	1674
SacII	CCGCGG	1 cut(s)	181
SalI	GTCGAC	1 cut(s)	938
SatI	GCNGC	8 cut(s)	181, 872, 1464, 1593, 1680, 1879, 2007, 2046
Sau96I	GGNCC	4 cut(s)	172, 808, 1876, 2242
SchI	GAGTC	3 cut(s)	271, 293, 611
ScrFI	CCNGG	3 cut(s)	1280, 1507, 1573
SfaNI	GCATC	8 cut(s)	658, 993, 1235, 1279, 1342, 1375, 1927, 2169
Sfr303I	CCGCGG	1 cut(s)	181
SfuI	TTCGAA	1 cut(s)	536
SgrBI	CCGCGG	1 cut(s)	181
SinI	GGWCC	2 cut(s)	808, 2242
SmiMI	CAYNNNNRTG	1 cut(s)	1674
SmlI	CTYRAG	6 cut(s)	218, 479, 599, 661, 1453, 1712
SmoI	CTYRAG	6 cut(s)	218, 479, 599, 661, 1453, 1712
SphI	GCATGC	2 cut(s)	1971, 2006
SspI	AATATT	1 cut(s)	2330
SspMI	CTAG	7 cut(s)	732, 1143, 1370, 1428, 1446, 1683, 2154
StyD4I	CCNGG	3 cut(s)	1278, 1505, 1571
StyI	CCWWGG	2 cut(s)	414, 1554
TaaI	ACNGT	3 cut(s)	897, 1698, 2348
TaiI	ACGT	2 cut(s)	1139, 2280
TatI	WGTACW	1 cut(s)	2296
TauI	GCSGC	5 cut(s)	183, 1682, 1881, 2009, 2048
TfiI	GAWTC	6 cut(s)	7, 980, 1126, 2039, 2416, 2495
TscAI	CASTG	7 cut(s)	923, 1048, 1215, 2093, 2353, 2367, 2449
TseFI	GTSAC	4 cut(s)	274, 709, 1982, 2438
TseI	GCWGC	3 cut(s)	871, 1463, 1592
Tsp45I	GTSAC	4 cut(s)	274, 709, 1982, 2438
TspDTI	ATGAA	5 cut(s)	1416, 2135, 2250, 2291, 2498
TspGWI	ACGGA	3 cut(s)	716, 1309, 2411
TspRI	CASTG	7 cut(s)	923, 1048, 1215, 2093, 2353, 2367, 2449
Tth111I	GACNNNGTC	2 cut(s)	280, 302
Vha464I	CTTAAG	1 cut(s)	661
VpaK11BI	GGWCC	2 cut(s)	808, 2242
XapI	RAATTY	6 cut(s)	38, 394, 538, 1246, 1324, 2065
XbaI	TCTAGA	1 cut(s)	1369
XceI	RCATGY	9 cut(s)	445, 551, 946, 1264, 1767, 1860, 1971, 2006, 2532
XmiI	GTMKAC	1 cut(s)	939
XmnI	GAANNNNTTC	1 cut(s)	1130
XspI	CTAG	7 cut(s)	732, 1143, 1370, 1428, 1446, 1683, 2154
Zsp2I	ATGCAT	3 cut(s)	673, 1357, 1858

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.