Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001866

Physical Location & Seq

Genomic Coordinates Forward (+)

12072476 .. 12079403

Gene Structure 6928 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00090270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGCCGGCCGGTATTATGATTCCAGCGAGAAATATGCCTTCGATGATGGGAAGCAATGGGAATCTTGGTGGGTATGGATCATCTTCAGGACTTACTCTTGGTCAGCCAAACATGATGGAAGGTGGAGGTCATTTCGGAAACCCACTGGACATGATGGCCACTCAGAACACATCAGAAAGCGAGCTGAATGCACGTCTCGGTGATGATTTCGACAGTGCCACAAAATCTGGCAGCGAGAACCTGGAAGGAGGTGGCTCCGGCGACGATGGACAAGACCCTCGCCCCAACAAGAAGAAGAGATATCACCGCCACACCCAGCACCAGATCCAAGAAATGGAAGCTTTCTTCAAGGAGTGTCCACACCCTGATGACAAGCAAAGGAAGGAATTGAGTCGAGAGTTAGGTTTGGAACCATTACAAGTTAAATTTTGGTTCCAAAACAAGCGCACCCAGATGAAGACGCAGCATGAGAGGCATGAGAACACGAGCCTTCGTAATGAAAATGAAAAGCTTCGAGCTGATAACATGAGATATCGAGATGCACTGAGCAATGCATCATGTCCTAATTGCGGAGGCCCAACGGCTATTGGAGAGATGTCCTTCGATGAGCATCATTTGAGACTTGAAAATGCTCGATTACGAGAAGAGATTGATAGAATATCAGCAATTGCTGCTAAATACGTCGGCAAGCCAGTGGGGAACTACCCTCTTATATCTTCACCAGTTCCTCCTAACCGCAGTCCACTTGATCTTGGAGTGGGAAACATGGGGACCAGTCCTCACCACAGCTTCTTATTAAACTCAGACATGTATGCTACAGGAGACATTATGCGGTCAATCGGTGCACCTAGTGAGGCAGATAAGCCCATGATTATTGAGCTTGCAGTTGCAGCTATGGAGGAGCTAATCCGAATGGCTCAAATTGGTGAACCTTTGTGGATGAATAGCATTGATGGATCAACTACTGTGCTCAACGAGGATGAGTACATTCGAACATTTCCTCGTGGAATTGTGCCAAAACAATCTGGATTCAAATGTGAAGCTTCGCGGGAGAGTGCTGTTGTCATTATGAACCACAATAACCTTGTTGAGATTCTCATGGATGTGAATCAATGGTCAACTGTATTTTCGGGGATTGTATCAAGAGCTATGACTCTGGATGTGCTATCAACTGGAGTAGCAGGAAACTACAATGGAGCCTTGCAAGTGATGACAGCCGAATTTCAAGTACCATCTCCATTGGTTCCAACTCGTGAGAGTTATTTTGTGAGGTACTGTAAGCAGCATGTCGATGGCACTTGGGCAGTGGTTGATGTCTCCTTGGACAATCAACGCGGCTATACTTCTGCTCTGAAATCATGCCAGAGAAGACCTTCTGGTTGTCTCATCCAAGAAATGCCAAATGGATACTCAAAGGTTACATGGATTGAGCATGTAGAAGTGGATGAAAGAGGTGTTCACAATCTGTACAAGCAGCTGGTCAACTCTGGCAATGCATTCGGGGCAAAAAGATGGGTTGCGACCTTGGACCGCCAATGTGAGCGTCTCGCCAGCGCCTTGGCGACAAACATCCCTACCGGTGATGTTGGTGTGATAACAACTCAAGAAGGGAGGAAGAGCATGTTGAAGCTGGCCGAGAGAATGGTTATAAGTTTCTGTGCTGGAGTGAGTGCATCTACCACTCATACATGGACGACGCTCTCTGGAACAGGGGCTGATGATGTTCGTGTGATGACCCGAAAGAGTGTGGATGATCCAGGAAGGCCTCCGGGCATTGTGCTCAGCGCTGCAACCTCATTCTGGCTTCCTGTCCCCCCCAAAAGGGTCTTTGAGTTCCTTCGTGATGAGAACTCACGAAATGAGTGGGATATCCTCTCCAATGGTGGCGTCGTGCAAGAAATGGCGCACATAGCCAACGGACGTGACACTGGGAACTGCGTGTCACTACTTCGAGTAAATAGTGCCAATTCGAGTCAGAGCAACATGCTCATACTGCAGGAGAGCTGCACTGACTCAACGGCATCATATGTAATCTATGCCCCTGTGGACATAGTTGCTATGAATGTCGTTCTGAATGGCAGTGACCCTGACTATGTTGCCCTGCTGCCATCTGGGTTTGCTATACTCCCAGATGGAACTAGCGGGAGCAACGGGGAAGGTGAATCTGGAGGGTCCTTGTTGACTGTTGCCTTCCAGATCCTGGTGGATTCAGTTCCCACCGCAAAGCTGTCTCTGGGGTCTGTTGCAACAGTCAACAACCTCATAGCTTGCACAGTCGAGCGGATCAAGGCCTCATTGTCCTTGGACAATGAAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0045595 GO:0045596 GO:0048367 GO:0048437 GO:0048468 GO:0048497 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0090700 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox START

Protein Analysis

772

Amino Acids

84.1

Weight (kDa)

5.5

Isoelectric Point (pI)

39.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	98 - 153	1.1e-18	Homeodomain
START	PF01852	294 - 515	2.2e-53	START domain
PDF2_C	PF25797	534 - 767	2e-101	PDF2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230
fragaria_vesca	FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390
malus_domestica	MD09G1107300.v1.1 MD17G1095000.v1.1
prunus_persica	Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1
pyrus_communis	pycom09g02950 pycom17g09140
rosa_chinensis	RchiOBHm_Chr2g0159731
rosa_laevigata	RLG00000021177
rosa_multiflora	Rmu_sc0004966.1_g000003
rosa_roxburghii	Rroxscaffold_2G00090270 Rroxscaffold_2G00090370
rosa_rugosa	Rorug02G0478100 Rorug02G0478200 Rorug02G0478300
rosa_samantha	Rh2AG544900 Rh2BG557700 Rh2CG528000 Rh2DG567200
rosa_wichuraiana	Rw2G045060 Rw3G015270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1649
AccB7I	CCANNNNNTGG	2 cut(s)	334, 2200
AccBSI	CCGCTC	1 cut(s)	2281
AccII	CGCG	2 cut(s)	1048, 1335
AclWI	GGATC	6 cut(s)	85, 319, 964, 1748, 2191, 2291
AcoI	YGGCCR	3 cut(s)	6, 156, 1632
AcsI	RAATTY	2 cut(s)	425, 1220
AcuI	CTGAAG	1 cut(s)	69
AcyI	GRCGYC	1 cut(s)	1887
AdeI	CACNNNGTG	1 cut(s)	851
AfaI	GTAC	4 cut(s)	986, 1230, 1274, 1469
AfeI	AGCGCT	1 cut(s)	1786
AfiI	CCNNNNNNNGG	6 cut(s)	334, 569, 1800, 1821, 1822, 2200
AflIII	ACRYGT	1 cut(s)	807
AgeI	ACCGGT	1 cut(s)	1577
AgsI	TTSAA	5 cut(s)	349, 626, 1033, 1226, 1627
AjiI	CACGTC	2 cut(s)	194, 1922
AjnI	CCWGG	3 cut(s)	240, 1756, 2199
Alw21I	GWGCWC	3 cut(s)	847, 972, 1782
Alw26I	GTCTC	7 cut(s)	200, 613, 816, 1321, 1388, 1550, 2235
Alw44I	GTGCAC	1 cut(s)	843
AlwI	GGATC	6 cut(s)	85, 319, 964, 1748, 2191, 2291
AlwNI	CAGNNNCTG	2 cut(s)	1715, 2200
Aor51HI	AGCGCT	1 cut(s)	1786
AoxI	GGCC	6 cut(s)	6, 156, 574, 1632, 1763, 2289
ApaLI	GTGCAC	1 cut(s)	843
ApeKI	GCWGC	9 cut(s)	231, 463, 671, 890, 1282, 1474, 1787, 2004, 2104
ApoI	RAATTY	2 cut(s)	425, 1220
AsiGI	ACCGGT	1 cut(s)	1577
Asp700I	GAANNNNTTC	2 cut(s)	510, 1372
AspLEI	GCGC	4 cut(s)	447, 1556, 1787, 1906
AspS9I	GGNCC	4 cut(s)	575, 771, 1528, 2172
AsuC2I	CCSGG	1 cut(s)	1770
AsuHPI	GGTGA	7 cut(s)	212, 296, 711, 773, 938, 1592, 2171
AsuII	TTCGAA	1 cut(s)	991
AvaII	GGWCC	3 cut(s)	771, 1528, 2172
BaeGI	GKGCMC	1 cut(s)	847
BalI	TGGCCA	1 cut(s)	158
BauI	CACGAG	3 cut(s)	484, 1002, 1251
BbsI	GAAGAC	2 cut(s)	464, 1375
Bbv12I	GWGCWC	3 cut(s)	847, 972, 1782
BbvI	GCAGC	9 cut(s)	243, 475, 658, 902, 1294, 1486, 1774, 1991, 2091
BceAI	ACGGC	2 cut(s)	597, 2034
BcgI	CGANNNNNNTGC	6 cut(s)	16, 50, 170, 204, 1480, 1514
BciT130I	CCWGG	3 cut(s)	242, 1758, 2201
BciVI	GTATCC	1 cut(s)	1400
BcnI	CCSGG	1 cut(s)	1770
BcoDI	GTCTC	7 cut(s)	200, 613, 816, 1321, 1388, 1550, 2235
BfaI	CTAG	2 cut(s)	849, 2139
BfmI	CTRYAG	2 cut(s)	816, 1994
BfoI	RGCGCY	2 cut(s)	1557, 1788
BfuI	GTATCC	1 cut(s)	1400
BlpI	GCTNAGC	1 cut(s)	1781
Bme1390I	CCNGG	4 cut(s)	242, 1758, 1770, 2201
Bme18I	GGWCC	3 cut(s)	771, 1528, 2172
BmgBI	CACGTC	2 cut(s)	194, 1922
BmgT120I	GGNCC	4 cut(s)	575, 771, 1528, 2172
BmiI	GGNNCC	7 cut(s)	256, 411, 434, 772, 1198, 1245, 2173
BmrFI	CCNGG	4 cut(s)	242, 1758, 1770, 2201
BmrI	ACTGGG	1 cut(s)	1938
BmsI	GCATC	5 cut(s)	529, 563, 619, 1682, 2030
BmuI	ACTGGG	1 cut(s)	1938
BpiI	GAAGAC	2 cut(s)	464, 1375
BpmI	CTGGAG	3 cut(s)	1194, 1683, 2187
Bpu1102I	GCTNAGC	1 cut(s)	1781
Bpu14I	TTCGAA	1 cut(s)	991
BpuEI	CTTGAG	1 cut(s)	1587
BpuMI	CCSGG	1 cut(s)	1770
BsaBI	GATNNNNATC	2 cut(s)	609, 1107
BsaHI	GRCGYC	1 cut(s)	1887
BsaJI	CCNNGG	4 cut(s)	1320, 1524, 1557, 2301
BsaWI	WCCGGW	1 cut(s)	1577
Bsc4I	CCNNNNNNNGG	6 cut(s)	334, 569, 1800, 1821, 1822, 2200
Bse118I	RCCGGY	3 cut(s)	4, 8, 1577
Bse1I	ACTGG	6 cut(s)	150, 692, 722, 774, 1177, 1933
Bse3DI	GCAATG	3 cut(s)	61, 556, 1498
Bse8I	GATNNNNATC	2 cut(s)	609, 1107
BseBI	CCWGG	3 cut(s)	242, 1758, 2201
BseDI	CCNNGG	4 cut(s)	1320, 1524, 1557, 2301
BseGI	GGATG	8 cut(s)	945, 985, 1108, 1165, 1386, 1450, 1569, 1756
BseJI	GATNNNNATC	2 cut(s)	609, 1107
BseLI	CCNNNNNNNGG	6 cut(s)	334, 569, 1800, 1821, 1822, 2200
BseMI	GCAATG	3 cut(s)	61, 556, 1498
BseMII	CTCAG	4 cut(s)	176, 536, 816, 1795
BseNI	ACTGG	6 cut(s)	150, 692, 722, 774, 1177, 1933
BseRI	GAGGAG	1 cut(s)	914
BseSI	GKGCMC	1 cut(s)	847
BseX3I	CGGCCG	1 cut(s)	6
BseXI	GCAGC	9 cut(s)	243, 475, 658, 902, 1294, 1486, 1774, 1991, 2091
BseYI	CCCAGC	1 cut(s)	315
BsgI	GTGCAG	1 cut(s)	1990
Bsh1236I	CGCG	2 cut(s)	1048, 1335
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	6 cut(s)	8, 158, 576, 1634, 1765, 2291
BshTI	ACCGGT	1 cut(s)	1577
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	3 cut(s)	847, 972, 1782
BsiSI	CCGG	5 cut(s)	5, 9, 258, 1578, 1769
BslFI	GGGAC	2 cut(s)	784, 1796
BslI	CCNNNNNNNGG	6 cut(s)	334, 569, 1800, 1821, 1822, 2200
BsmAI	GTCTC	7 cut(s)	200, 613, 816, 1321, 1388, 1550, 2235
BsmBI	CGTCTC	2 cut(s)	200, 1550
BsmFI	GGGAC	2 cut(s)	784, 1796
BsmI	GAATGC	2 cut(s)	193, 1496
BsnI	GGCC	6 cut(s)	8, 158, 576, 1634, 1765, 2291
Bsp119I	TTCGAA	1 cut(s)	991
Bsp1286I	GDGCHC	3 cut(s)	847, 972, 1782
Bsp1407I	TGTACA	1 cut(s)	1467
Bsp143I	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
Bsp1720I	GCTNAGC	1 cut(s)	1781
BspANI	GGCC	6 cut(s)	8, 158, 576, 1634, 1765, 2291
BspCNI	CTCAG	4 cut(s)	175, 537, 815, 1794
BspFNI	CGCG	2 cut(s)	1048, 1335
BspLI	GGNNCC	7 cut(s)	256, 411, 434, 772, 1198, 1245, 2173
BspMAI	CTGCAG	1 cut(s)	1998
BspPI	GGATC	6 cut(s)	85, 319, 964, 1748, 2191, 2291
BspQI	GCTCTTC	1 cut(s)	1610
BspT104I	TTCGAA	1 cut(s)	991
BsrBI	CCGCTC	1 cut(s)	2281
BsrDI	GCAATG	3 cut(s)	61, 556, 1498
BsrFI	RCCGGY	3 cut(s)	4, 8, 1577
BsrGI	TGTACA	1 cut(s)	1467
BsrI	ACTGG	6 cut(s)	150, 692, 722, 774, 1177, 1933
BssAI	RCCGGY	3 cut(s)	4, 8, 1577
BssECI	CCNNGG	4 cut(s)	1320, 1524, 1557, 2301
BssMI	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
BssNI	GRCGYC	1 cut(s)	1887
BssSI	CACGAG	3 cut(s)	484, 1002, 1251
BssT1I	CCWWGG	4 cut(s)	1320, 1524, 1557, 2301
Bst2BI	CACGAG	3 cut(s)	484, 1002, 1251
Bst2UI	CCWGG	3 cut(s)	242, 1758, 2201
Bst4CI	ACNGT	7 cut(s)	215, 967, 1123, 1277, 2185, 2251, 2275
Bst6I	CTCTTC	3 cut(s)	290, 639, 1610
BstACI	GRCGYC	1 cut(s)	1887
BstAPI	GCANNNNNTGC	1 cut(s)	671
BstAUI	TGTACA	1 cut(s)	1467
BstBI	TTCGAA	1 cut(s)	991
BstC8I	GCNNGC	7 cut(s)	6, 182, 689, 882, 1552, 1632, 2269
BstDEI	CTNAG	4 cut(s)	162, 545, 802, 1781
BstF5I	GGATG	8 cut(s)	945, 985, 1108, 1165, 1386, 1450, 1569, 1756
BstFNI	CGCG	2 cut(s)	1048, 1335
BstH2I	RGCGCY	2 cut(s)	1557, 1788
BstHHI	GCGC	4 cut(s)	447, 1556, 1787, 1906
BstKTI	GATC	7 cut(s)	80, 327, 751, 959, 1756, 2199, 2286
BstMAI	GTCTC	7 cut(s)	200, 613, 816, 1321, 1388, 1550, 2235
BstMBI	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	6 cut(s)	472, 671, 890, 1502, 1910, 1993
BstNI	CCWGG	3 cut(s)	242, 1758, 2201
BstNSI	RCATGY	5 cut(s)	811, 1289, 1436, 1624, 1987
BstSCI	CCNGG	4 cut(s)	240, 1756, 1768, 2199
BstSFI	CTRYAG	2 cut(s)	816, 1994
BstSLI	GKGCMC	1 cut(s)	847
BstUI	CGCG	2 cut(s)	1048, 1335
BstV1I	GCAGC	9 cut(s)	243, 475, 658, 902, 1294, 1486, 1774, 1991, 2091
BstV2I	GAAGAC	2 cut(s)	464, 1375
BstX2I	RGATCY	2 cut(s)	324, 2196
BstXI	CCANNNNNNTGG	1 cut(s)	1558
BstYI	RGATCY	2 cut(s)	324, 2196
BstZI	CGGCCG	1 cut(s)	6
BsuI	GTATCC	1 cut(s)	1400
BsuRI	GGCC	6 cut(s)	8, 158, 576, 1634, 1765, 2291
BtrI	CACGTC	2 cut(s)	194, 1922
BtsCI	GGATG	8 cut(s)	945, 985, 1108, 1165, 1386, 1450, 1569, 1756
BtsI	GCAGTG	2 cut(s)	1311, 2086
BtsIMutI	CAGTG	8 cut(s)	143, 220, 542, 699, 1311, 1926, 2007, 2086
Cac8I	GCNNGC	7 cut(s)	6, 182, 689, 882, 1552, 1632, 2269
CaiI	CAGNNNCTG	2 cut(s)	1715, 2200
CfoI	GCGC	4 cut(s)	447, 1556, 1787, 1906
Cfr10I	RCCGGY	3 cut(s)	4, 8, 1577
Cfr13I	GGNCC	4 cut(s)	575, 771, 1528, 2172
CseI	GACGC	4 cut(s)	469, 1532, 1705, 1876
Csp6I	GTAC	4 cut(s)	985, 1229, 1273, 1468
CspAI	ACCGGT	1 cut(s)	1577
CviQI	GTAC	4 cut(s)	985, 1229, 1273, 1468
DdeI	CTNAG	4 cut(s)	162, 545, 802, 1781
DpnI	GATC	7 cut(s)	79, 326, 750, 958, 1755, 2198, 2285
DpnII	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
DraIII	CACNNNGTG	1 cut(s)	851
EaeI	YGGCCR	3 cut(s)	6, 156, 1632
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	3 cut(s)	290, 639, 1610
EarI	CTCTTC	3 cut(s)	290, 639, 1610
EclXI	CGGCCG	1 cut(s)	6
Eco130I	CCWWGG	4 cut(s)	1320, 1524, 1557, 2301
Eco147I	AGGCCT	2 cut(s)	1765, 2291
Eco32I	GATATC	3 cut(s)	302, 533, 1870
Eco47I	GGWCC	3 cut(s)	771, 1528, 2172
Eco47III	AGCGCT	1 cut(s)	1786
Eco52I	CGGCCG	1 cut(s)	6
Eco57I	CTGAAG	1 cut(s)	69
EcoO109I	RGGNCCY	1 cut(s)	2172
EcoRII	CCWGG	3 cut(s)	240, 1756, 2199
EcoRV	GATATC	3 cut(s)	302, 533, 1870
EcoT14I	CCWWGG	4 cut(s)	1320, 1524, 1557, 2301
EcoT22I	ATGCAT	2 cut(s)	556, 1498
ErhI	CCWWGG	4 cut(s)	1320, 1524, 1557, 2301
Esp3I	CGTCTC	2 cut(s)	200, 1550
FaqI	GGGAC	2 cut(s)	784, 1796
FauI	CCCGC	2 cut(s)	1041, 2135
FauNDI	CATATG	1 cut(s)	2026
FokI	GGATG	8 cut(s)	952, 992, 1115, 1172, 1373, 1457, 1556, 1763
FspBI	CTAG	2 cut(s)	849, 2139
GlaI	GCGC	4 cut(s)	446, 1555, 1786, 1905
GsaI	CCCAGC	1 cut(s)	319
GsuI	CTGGAG	3 cut(s)	1194, 1683, 2187
HaeII	RGCGCY	2 cut(s)	1557, 1788
HaeIII	GGCC	6 cut(s)	8, 158, 576, 1634, 1765, 2291
HapII	CCGG	5 cut(s)	5, 9, 258, 1578, 1769
HgaI	GACGC	4 cut(s)	469, 1532, 1705, 1876
HhaI	GCGC	4 cut(s)	447, 1556, 1787, 1906
Hin1I	GRCGYC	1 cut(s)	1887
Hin6I	GCGC	4 cut(s)	445, 1554, 1785, 1904
HinP1I	GCGC	4 cut(s)	445, 1554, 1785, 1904
HincII	GTYRAC	4 cut(s)	1119, 1483, 2181, 2254
HindII	GTYRAC	4 cut(s)	1119, 1483, 2181, 2254
HindIII	AAGCTT	3 cut(s)	339, 509, 1041
HpaII	CCGG	5 cut(s)	5, 9, 258, 1578, 1769
HphI	GGTGA	7 cut(s)	212, 296, 711, 773, 938, 1592, 2171
Hpy188I	TCNGA	8 cut(s)	137, 165, 175, 805, 911, 1353, 1977, 2073
Hpy99I	CGWCG	4 cut(s)	266, 686, 1699, 1892
HpyCH4III	ACNGT	7 cut(s)	215, 967, 1123, 1277, 2185, 2251, 2275
HpyCH4IV	ACGT	3 cut(s)	193, 681, 1921
HpyF10VI	GCNNNNNNNGC	6 cut(s)	472, 671, 890, 1502, 1910, 1993
HpyF3I	CTNAG	4 cut(s)	162, 545, 802, 1781
HpySE526I	ACGT	3 cut(s)	193, 681, 1921
Hsp92I	GRCGYC	1 cut(s)	1887
HspAI	GCGC	4 cut(s)	445, 1554, 1785, 1904
KroI	GCCGGC	1 cut(s)	4
KroNI	GCCGGC	1 cut(s)	6
Kzo9I	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
LguI	GCTCTTC	1 cut(s)	1610
LmnI	GCTCC	4 cut(s)	260, 901, 1196, 2145
Lsp1109I	GCAGC	9 cut(s)	243, 475, 658, 902, 1294, 1486, 1774, 1991, 2091
LweI	GCATC	5 cut(s)	529, 563, 619, 1682, 2030
MaeI	CTAG	2 cut(s)	849, 2139
MaeII	ACGT	3 cut(s)	193, 681, 1921
MaeIII	GTNAC	4 cut(s)	1417, 1922, 1941, 2081
MalI	GATC	7 cut(s)	79, 326, 750, 958, 1755, 2198, 2285
MbiI	CCGCTC	1 cut(s)	2281
MboI	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
MboII	GAAGA	9 cut(s)	75, 304, 307, 337, 469, 656, 708, 1380, 1627
MfeI	CAATTG	1 cut(s)	666
MflI	RGATCY	2 cut(s)	324, 2196
MhlI	GDGCHC	3 cut(s)	847, 972, 1782
MlsI	TGGCCA	1 cut(s)	158
MluCI	AATT	8 cut(s)	386, 425, 565, 666, 921, 1008, 1220, 1966
MluNI	TGGCCA	1 cut(s)	158
MlyI	GAGTC	4 cut(s)	400, 1147, 1981, 2006
MmeI	TCCRAC	1 cut(s)	1271
Mox20I	TGGCCA	1 cut(s)	158
Mph1103I	ATGCAT	2 cut(s)	556, 1498
MroNI	GCCGGC	1 cut(s)	4
MroXI	GAANNNNTTC	2 cut(s)	510, 1372
MscI	TGGCCA	1 cut(s)	158
MseI	TTAA	2 cut(s)	423, 797
MslI	CAYNNNNRTG	4 cut(s)	366, 452, 1103, 1290
Msp20I	TGGCCA	1 cut(s)	158
MspA1I	CMGCKG	1 cut(s)	1477
MspI	CCGG	5 cut(s)	5, 9, 258, 1578, 1769
MspR9I	CCNGG	4 cut(s)	242, 1758, 1770, 2201
MunI	CAATTG	1 cut(s)	666
Mva1269I	GAATGC	2 cut(s)	193, 1496
MvaI	CCWGG	3 cut(s)	242, 1758, 2201
MvnI	CGCG	2 cut(s)	1048, 1335
MwoI	GCNNNNNNNGC	6 cut(s)	472, 671, 890, 1502, 1910, 1993
NaeI	GCCGGC	1 cut(s)	6
NciI	CCSGG	1 cut(s)	1770
NdeI	CATATG	1 cut(s)	2026
NdeII	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
NgoMIV	GCCGGC	1 cut(s)	4
NlaIV	GGNNCC	7 cut(s)	256, 411, 434, 772, 1198, 1245, 2173
NmeAIII	GCCGAG	1 cut(s)	1660
NmuCI	GTSAC	3 cut(s)	1922, 1941, 2081
NsiI	ATGCAT	2 cut(s)	556, 1498
NspI	RCATGY	5 cut(s)	811, 1289, 1436, 1624, 1987
NspV	TTCGAA	1 cut(s)	991
PceI	AGGCCT	2 cut(s)	1765, 2291
PciI	ACATGT	1 cut(s)	807
PciSI	GCTCTTC	1 cut(s)	1610
PctI	GAATGC	2 cut(s)	193, 1496
PdiI	GCCGGC	1 cut(s)	6
PdmI	GAANNNNTTC	2 cut(s)	510, 1372
PfeI	GAWTC	7 cut(s)	19, 61, 1029, 1093, 1108, 2162, 2207
PflMI	CCANNNNNTGG	2 cut(s)	334, 2200
PfoI	TCCNGGA	1 cut(s)	1756
PinAI	ACCGGT	1 cut(s)	1577
PleI	GAGTC	4 cut(s)	399, 1147, 1980, 2006
PpsI	GAGTC	4 cut(s)	399, 1147, 1980, 2006
PpuMI	RGGWCCY	1 cut(s)	2172
PscI	ACATGT	1 cut(s)	807
PsiI	TTATAA	1 cut(s)	1649
Psp5II	RGGWCCY	1 cut(s)	2172
Psp6I	CCWGG	3 cut(s)	240, 1756, 2199
PspFI	CCCAGC	1 cut(s)	315
PspGI	CCWGG	3 cut(s)	240, 1756, 2199
PspN4I	GGNNCC	7 cut(s)	256, 411, 434, 772, 1198, 1245, 2173
PspPI	GGNCC	4 cut(s)	575, 771, 1528, 2172
PspPPI	RGGWCCY	1 cut(s)	2172
PstI	CTGCAG	1 cut(s)	1998
PstNI	CAGNNNCTG	2 cut(s)	1715, 2200
PsuI	RGATCY	2 cut(s)	324, 2196
PvuII	CAGCTG	1 cut(s)	1477
RsaI	GTAC	4 cut(s)	986, 1230, 1274, 1469
RsaNI	GTAC	4 cut(s)	985, 1229, 1273, 1468
RseI	CAYNNNNRTG	4 cut(s)	366, 452, 1103, 1290
SapI	GCTCTTC	1 cut(s)	1610
SaqAI	TTAA	2 cut(s)	423, 797
Sau3AI	GATC	7 cut(s)	77, 324, 748, 956, 1753, 2196, 2283
Sau96I	GGNCC	4 cut(s)	575, 771, 1528, 2172
SchI	GAGTC	4 cut(s)	400, 1147, 1981, 2006
ScrFI	CCNGG	4 cut(s)	242, 1758, 1770, 2201
SduI	GDGCHC	3 cut(s)	847, 972, 1782
SfaNI	GCATC	5 cut(s)	529, 563, 619, 1682, 2030
SfcI	CTRYAG	2 cut(s)	816, 1994
SfuI	TTCGAA	1 cut(s)	991
SinI	GGWCC	3 cut(s)	771, 1528, 2172
SmiMI	CAYNNNNRTG	4 cut(s)	366, 452, 1103, 1290
SmlI	CTYRAG	1 cut(s)	1602
SmoI	CTYRAG	1 cut(s)	1602
Sse9I	AATT	8 cut(s)	386, 425, 565, 666, 921, 1008, 1220, 1966
SseBI	AGGCCT	2 cut(s)	1765, 2291
SspMI	CTAG	2 cut(s)	849, 2139
StuI	AGGCCT	2 cut(s)	1765, 2291
StyD4I	CCNGG	4 cut(s)	240, 1756, 1768, 2199
StyI	CCWWGG	4 cut(s)	1320, 1524, 1557, 2301
TaaI	ACNGT	7 cut(s)	215, 967, 1123, 1277, 2185, 2251, 2275
TaiI	ACGT	3 cut(s)	196, 684, 1924
TasI	AATT	8 cut(s)	386, 425, 565, 666, 921, 1008, 1220, 1966
TatI	WGTACW	2 cut(s)	984, 1467
TauI	GCSGC	1 cut(s)	1338
TfiI	GAWTC	7 cut(s)	19, 61, 1029, 1093, 1108, 2162, 2207
Tru1I	TTAA	2 cut(s)	423, 797
Tru9I	TTAA	2 cut(s)	423, 797
TscAI	CASTG	8 cut(s)	150, 220, 549, 699, 1311, 1933, 2014, 2086
TseFI	GTSAC	3 cut(s)	1922, 1941, 2081
TseI	GCWGC	9 cut(s)	231, 463, 671, 890, 1282, 1474, 1787, 2004, 2104
Tsp45I	GTSAC	3 cut(s)	1922, 1941, 2081
TspDTI	ATGAA	7 cut(s)	470, 513, 519, 956, 1085, 1461, 2075
TspGWI	ACGGA	1 cut(s)	1932
TspRI	CASTG	8 cut(s)	150, 220, 549, 699, 1311, 1933, 2014, 2086
Van91I	CCANNNNNTGG	2 cut(s)	334, 2200
VneI	GTGCAC	1 cut(s)	843
VpaK11BI	GGWCC	3 cut(s)	771, 1528, 2172
XapI	RAATTY	2 cut(s)	425, 1220
XceI	RCATGY	5 cut(s)	811, 1289, 1436, 1624, 1987
XmnI	GAANNNNTTC	2 cut(s)	510, 1372
XspI	CTAG	2 cut(s)	849, 2139
Zsp2I	ATGCAT	2 cut(s)	556, 1498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.