Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001866

Physical Location & Seq

Genomic Coordinates Forward (+)

60942963 .. 60947769

Gene Structure 4807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0478100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4092 bp

ATGTGTTGGAATGTGCGTGGAGCTGGAGGCAAGAAGTTTAGGTCAGCTATTCCTGATCTCATTTCTCTACATAAAGTTGATTTACTTATTATTTGTGAACCTAGAGTCCCTTTTGCTAGGGTTCAAAAATTTCTTGGTAATCATGGGTTTCCTAATGCTGAGATTGTTGAAGCCAGAGGTTTTTCTGGTGGTGTTTGGGTTTTATATGATAAGAATAAAATTGGTGTTAATTTTGTTGATAGTACTAACCAGTCAGTATCTGTTAAAGTCTCAATTGGTGGATTACATGATTGGGTGCTGACCGCTATCTATGCTAGTCCTACTACTTCAATTAGATGTCTACTATGGGAACATTTATCTCAGTTTGCTCTACACTGTCAATTGCCTTGGTTGGTTATTGGAGATTGTAATGAGCTATATAGTAGTGCGGATAAAAATGGAGGATCTTTAATTGGAAAATTTGGCGGTATGAGAAGATGGGTTGACAACTCTGGGATGATTGATCTAGGCTTTCGAGGTGCTGAATTTACTTGGCAAGACAAAAGGGTTAAGGAGCGGTTGGATCGAGGATTTTGCAATGATTCTTGGAGAGTTGCTTTTCCTCAAGCTTTTATCACTCATCTACCTAAGTTGAAGTCTACCTCTGATCATTGTCCTCTATTGCTTCAGTCTGAATCTTCCGTAACTTCTAATGGTGGGATGAAACCTTTCAGATTCCAAGCTATGTGGATGAGCCACTGTGATTATAATAAATTTGTTCTTGATCAATGGCAGAGTTATAATGGGGGTGTTCAACAAAAAGTTGATTGTTTGGCCAAGGATTTGCAGAAGTGGAATTCCAATGTTTTTGGTGATTTGTTTAAAAAGAAAAGGAGGATTTTAGCTCGAATTTCTGGTATTCAGAAATGTCTTGCTAAAAGTGATAACCCTTTCCTTGTAAACTTAGAGATTGAGCTACTGAAGGAATATGAAACCTTAAGGGATCAAGAAGCTATGTATTGGCAACAAAAATCTAGAGTCAAGTGGTTGCAGCATGGTGATCGAAATTCTAGATTTTTCCACATGTCCACTATGATTAGAAGGAGAAGAAACAGAATTGAGGGTTTGTTGGATATGGATGGGACTCTTTGTAATGATCTTGATGGTATGAAGTCTATTGCTGCCAAATTTTTTAAAAATCTCTTCACCTACAGTTCCTTTGATGATCTAAGATTTGTTATTCCAAGCCTTTTCCCTCCCTTAAGTGATAATGAGATGAATTGGATGAGTAGAAATATCACAAATTCTGAGGTGAAATTTGCTCTGTTTAATATCGGGAGTCTCAAAGCTCCAGGTGCAGATGGCTTCCCTGCGTTGTTCTACCAGAAACACTGGGATATCTGTGCTGTTGAAGTGTTTAATCTTGTAAACCAAGCCTTCTGCTCTGGAGTTATTCCCCATGGTCTGAATCACACTCTCATTACCTTAGTGCCAAAAACTGCTAGCCCTCAAGACATGGCTCTATTCAGACCCATTAGCCTTTGTAATACTCTTTATAAAGTTATTTCTAAGGTGATGGTGGCCAGAATTAGACCTTCCCTCAAGAATCTCATAAGCCCTAATCAAGTTAGCTTTGTTCCAGGGAGGCATATCATTGATAATATCATTATTGCTCAAGAGGTTCTACATAAGTTCCAGCACTCCTATGGTCAAAAAGGGTTTATGGCATGGAAGATTGATTTGTCTAAAGCTTATGATAGACTGAATTGGAATTTTATCGAGTCTGTGCTATATGAAGCTCAATTTCCTGATCGTATTGTGAAATTAATTATGCAATGTGTTTCTACAACTAGTTTCCAAGTCTGTGTCAATGGTGAGCTGTCTGAAAATTTTACTGCTAGCAGGGGAATTAGGCAAGGGGATCCCCTGTCTCCTTACCTGTTTGTTTTATGTATGGAAAAGTTGTCTCATCTAATCACTTCTGCTGTGGAGGTTGGGCAGTGGAAACCTGTGCGTGCTTCTGGTTCTGGACCTTTGATTTCCCACTTATTCTTTGCAGATGATCTTATTCTTTTTGCGGAGGCTAGTTGTGAGCAAGCAACTGTTCTTAAAAAATGCTTAGATGTTTTTTGCGCTCTATCTGGACAAGAGGTTAGTTTCAGCAAGTCACTTCTTTATTGCTCTCCTAATACTTGTAAATCTTTGGCTGGAAACATAAGTAGAATCTGTGGCTCTTCTCTAACTAATAATCTTGGCAAGTATCTCGGAATGCCGTTGATCCACACAAAGGTAAATAAACAGACTTATGCTCCTATTATTGAGAAAGTTCAGAGTAGACTAGCTGGATGGAAAAGTAAGTGTCTGAACATGGCTGGTAGGCTCACTCTTATAAATTCTGTGTGCTCTGCTATTCCAGTTTATGCTATGCAAACTGTGAAGCTACCTATATCTCTATGTGATTCTTTGGATAAATTAAACAGGGACTTTCTTTGGGGTGATTCTGAAGCTAAGAAAAGGGTGCATCTAGCCAATTGGGATATGGTTTGTCGTCCTAAGGAGTATGGTGGCTTGGGAATCAAGAGTACTTCGCAAATGAATCAAGCTCTTCTTGCAAAAATTGGGTGGAGATTAACTCAAGGAGGTAATGGATTGTGGAGTAAGGTTCTCCATCGGAAGTATGTGAAGACTAATTCCTTACTTCATTCTCAGTATGTGTGCCCTTCTAGGTGTTCTAGTACATGGAGAAGTGTCCTTTTTGGTACTAAATTGCTTAAGCAGGGATTGTCATGGAGGATTGGTGATGGTAATACTGTGAATTTTTGGACTGACATTTGGACTCCAAGTGGACCTTTGATTAAGGATGCTGTAAGCCCTGGAAATGTTGATTTAAATCTGAAGGTGAAGGATTTTTGGAACGGTGATGGTTGGAACCTAGATTTGATAAGACAATGTGTGAGTGAGGATTCCATTAGCGAAATTATCCAAGTCCCTGCTGGTTTCCTTGGTGGTGGACATGATAAATTGATCTGGGGGGCTACTTCAAATGGTAATTTCACTGTTAAATCTGCCTACCTTACGCTTATAAAGGAGAAAAATTTTGATAGTTATCCTTGGTTCTTTTGTTGGAAACTATGCATCCCTCCCAAGTTGAAGACTTTTATGTGGTGTTTCTGTCACCAGAAACTTTTGTCCAATGTTGAGAGGGCTAAAAGGCATATGACTATGGATTCTTCTTGCCCTTTATGCCATGAACAACCTGAAACTATGATCCATATGCTTAGAGACTGTCAGTTTGCTCGTGTTGTTTGGAATGATATTGTTTGTCTTGATACGGTTGCTAGAAGCATGCAGCTAGATTGGAGTAGTTGGTGTGCTGCTAATTTACATTATCATAGGCGGTGTTATGGGGATTTGAAGTGGAGTACTGTGTTTGTCTTCACCTGTTGGTACTTATGGAAATGGAGAAATAAGGTAGTGTTTGAGAATGACTTTCATATGCCTTTGAATCCTTGTAAGGTAATCATTGATGTGGCTAAGGAATGGTGTGCTGCAAATTCTGGTAATTCAGGTGATTCTCCTAAAAAAACAATTACTCTTAAGTGGAATGCTCCTCCTGTGCACTGCTTTAAACTTAATGTTGATGGAGCAAGGAATCAAATGGGTAAAATTGGGGCAGGTGGTGTAATTAGGAGTCATGATGGCTCTTGGGTTGCTGGATTTTATGCCAACCTGGGAAGTGGCTCTGTTCTCCAATCTGAGGCATGGGGTCTGTTGTTGGGGCTAAAATTGGCCTATGATAATCAATGCTCCCATTTGTATGTTGAAAGTGATTCTGAAACCCTTATTAACATGGTAAAGAATGGAGTGGATGAAGTGCATCCTCTAAAGACTATCCTCAATTGTTGCTCATTCTTGCAGCAAAAGTTCCTGAGCTTTGATATTAGACACACCTATAGAGAAGTGAATGCAGTTGCAGATATCCTATCCAAGGATGGGTTGCAGGCTGAAGCTGGGGTGCATGTCATGTTGCATCCTCCTCCACAAGTCATTAACTCTCTTCTTGATGACTTGTGTGAATTTCCTAGAGTTAGGATCGTGAATTCCGAAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0045595 GO:0045596 GO:0048367 GO:0048437 GO:0048468 GO:0048497 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0090700 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox START

Protein Analysis

1363

Amino Acids

154.17

Weight (kDa)

9.0

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo_endo_phos	PF03372	1 - 217	3.2e-11	Endonuclease/Exonuclease/phosphatase family
RVT_1	PF00078	499 - 750	3.8e-30	Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT	PF13966	1011 - 1096	8e-17	zinc-binding in reverse transcriptase
RVT_3	PF13456	1205 - 1324	2.1e-22	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230 AT1G05230
fragaria_vesca	FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390 FvH4_6g43390
malus_domestica	MD09G1107300.v1.1 MD17G1095000.v1.1
prunus_persica	Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1 Prupe.3G218500_v2.0.a1
pyrus_communis	pycom09g02950 pycom17g09140
rosa_chinensis	RchiOBHm_Chr2g0159731
rosa_laevigata	RLG00000021177
rosa_multiflora	Rmu_sc0004966.1_g000003
rosa_roxburghii	Rroxscaffold_2G00090270 Rroxscaffold_2G00090370
rosa_rugosa	Rorug02G0478100 Rorug02G0478200 Rorug02G0478300
rosa_samantha	Rh2AG544900 Rh2BG557700 Rh2CG528000 Rh2DG567200
rosa_wichuraiana	Rw2G045060 Rw3G015270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	5 cut(s)	747, 780, 1536, 2369, 3062
AarI	CACCTGC	1 cut(s)	3644
Acc36I	ACCTGC	1 cut(s)	3644
AccBSI	CCGCTC	1 cut(s)	556
AccI	GTMKAC	3 cut(s)	340, 638, 2314
AciI	CCGC	6 cut(s)	303, 428, 465, 556, 2057, 3376
AclWI	GGATC	8 cut(s)	451, 570, 990, 1895, 1908, 2251, 3241, 4079
AcoI	YGGCCR	2 cut(s)	813, 1560
AcuI	CTGAAG	5 cut(s)	650, 980, 2502, 2894, 4005
AfaI	GTAC	6 cut(s)	244, 2563, 2716, 2740, 3403, 3428
AfiI	CCNNNNNNNGG	4 cut(s)	2266, 3736, 3967, 3972
AflII	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
AflIII	ACRYGT	1 cut(s)	1062
AhlI	ACTAGT	1 cut(s)	1829
AjnI	CCWGG	4 cut(s)	1330, 1618, 2851, 3708
AloI	GAACNNNNNNTCC	2 cut(s)	90, 122
Alw21I	GWGCWC	2 cut(s)	2384, 3600
Alw26I	GTCTC	5 cut(s)	274, 1325, 1914, 1950, 3255
Alw44I	GTGCAC	1 cut(s)	3596
AlwI	GGATC	8 cut(s)	451, 570, 990, 1895, 1908, 2251, 3241, 4079
AlwNI	CAGNNNCTG	1 cut(s)	260
AoxI	GGCC	3 cut(s)	813, 1560, 3768
ApaLI	GTGCAC	1 cut(s)	3596
ApeKI	GCWGC	6 cut(s)	1030, 1160, 3328, 3353, 3527, 3895
ArsI	GACNNNNNNTTYG	2 cut(s)	3254, 3286
AseI	ATTAAT	1 cut(s)	1805
Asp700I	GAANNNNTTC	1 cut(s)	707
AspLEI	GCGC	1 cut(s)	2114
AspS9I	GGNCC	2 cut(s)	2009, 2825
AsuNHI	GCTAGC	2 cut(s)	1481, 1877
AvaII	GGWCC	2 cut(s)	2009, 2825
AxyI	CCTNAGG	1 cut(s)	2532
BaeGI	GKGCMC	2 cut(s)	2699, 3600
BalI	TGGCCA	2 cut(s)	815, 1562
BamHI	GGATCC	1 cut(s)	1900
BauI	CACGAG	1 cut(s)	3276
BbsI	GAAGAC	3 cut(s)	2669, 3137, 3406
Bbv12I	GWGCWC	2 cut(s)	2384, 3600
BbvI	GCAGC	6 cut(s)	1042, 1147, 3340, 3340, 3514, 3907
BceAI	ACGGC	1 cut(s)	2236
BcgI	CGANNNNNNTGC	6 cut(s)	555, 589, 1022, 1056, 2224, 2258
BciT130I	CCWGG	4 cut(s)	1332, 1620, 2853, 3710
BclI	TGATCA	2 cut(s)	646, 763
BcoDI	GTCTC	5 cut(s)	274, 1325, 1914, 1950, 3255
BcuI	ACTAGT	1 cut(s)	1829
BfmI	CTRYAG	2 cut(s)	1189, 3931
BfrI	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
BfuAI	ACCTGC	1 cut(s)	3644
BisI	GCNGC	6 cut(s)	1031, 1161, 3329, 3354, 3528, 3896
BlsI	GCNGC	6 cut(s)	1032, 1162, 3330, 3355, 3529, 3897
BmcAI	AGTACT	3 cut(s)	244, 2563, 3403
Bme1390I	CCNGG	4 cut(s)	1332, 1620, 2853, 3710
Bme18I	GGWCC	2 cut(s)	2009, 2825
BmgT120I	GGNCC	2 cut(s)	2009, 2825
BmiI	GGNNCC	2 cut(s)	1902, 2909
BmrFI	CCNGG	4 cut(s)	1332, 1620, 2853, 3710
BmrI	ACTGGG	1 cut(s)	1381
BmsI	GCATC	5 cut(s)	2509, 2830, 3123, 3865, 4018
BmtI	GCTAGC	2 cut(s)	1485, 1881
BmuI	ACTGGG	1 cut(s)	1381
BpiI	GAAGAC	3 cut(s)	2669, 3137, 3406
BplI	GAGNNNNNCTC	2 cut(s)	2596, 2628
BpmI	CTGGAG	3 cut(s)	45, 1314, 1446
Bpu10I	CCTNAGC	2 cut(s)	3513, 3908
BpuEI	CTTGAG	5 cut(s)	588, 1473, 1565, 1638, 2598
BsaBI	GATNNNNATC	2 cut(s)	2868, 3084
BsaJI	CCNNGG	9 cut(s)	386, 816, 1438, 1619, 2851, 2980, 3089, 3709, 3966
BsaXI	ACNNNNNCTCC	4 cut(s)	2272, 2302, 2761, 2791
Bsc4I	CCNNNNNNNGG	4 cut(s)	2266, 3736, 3967, 3972
Bse1I	ACTGG	3 cut(s)	250, 1376, 2393
Bse21I	CCTNAGG	1 cut(s)	2532
Bse3DI	GCAATG	2 cut(s)	583, 1820
Bse8I	GATNNNNATC	2 cut(s)	2868, 3084
BseBI	CCWGG	4 cut(s)	1332, 1620, 2853, 3710
BseDI	CCNNGG	9 cut(s)	386, 816, 1438, 1619, 2851, 2980, 3089, 3709, 3966
BseJI	GATNNNNATC	2 cut(s)	2868, 3084
BseLI	CCNNNNNNNGG	4 cut(s)	2266, 3736, 3967, 3972
BseMI	GCAATG	2 cut(s)	583, 1820
BseMII	CTCAG	6 cut(s)	150, 374, 1278, 2699, 3726, 3899
BseNI	ACTGG	3 cut(s)	250, 1376, 2393
BseRI	GAGGAG	2 cut(s)	3579, 4005
BseSI	GKGCMC	2 cut(s)	2699, 3600
BseXI	GCAGC	6 cut(s)	1042, 1147, 3340, 3340, 3514, 3907
BseYI	CCCAGC	1 cut(s)	3989
BsgI	GTGCAG	1 cut(s)	1356
BshFI	GGCC	3 cut(s)	815, 1562, 3770
BsiHKAI	GWGCWC	2 cut(s)	2384, 3600
BslFI	GGGAC	4 cut(s)	92, 1135, 2474, 2951
BslI	CCNNNNNNNGG	4 cut(s)	2266, 3736, 3967, 3972
BsmAI	GTCTC	5 cut(s)	274, 1325, 1914, 1950, 3255
BsmFI	GGGAC	4 cut(s)	92, 1135, 2474, 2951
BsmI	GAATGC	3 cut(s)	2253, 3589, 3949
BsnI	GGCC	3 cut(s)	815, 1562, 3770
Bsp1286I	GDGCHC	3 cut(s)	2384, 2699, 3600
Bsp19I	CCATGG	1 cut(s)	1438
BspACI	CCGC	6 cut(s)	303, 428, 465, 556, 2057, 3376
BspANI	GGCC	3 cut(s)	815, 1562, 3770
BspCNI	CTCAG	6 cut(s)	151, 373, 1279, 2698, 3727, 3900
BspHI	TCATGA	1 cut(s)	3673
BspLI	GGNNCC	2 cut(s)	1902, 2909
BspMI	ACCTGC	1 cut(s)	3644
BspOI	GCTAGC	2 cut(s)	1485, 1881
BspPI	GGATC	8 cut(s)	451, 570, 990, 1895, 1908, 2251, 3241, 4079
BspQI	GCTCTTC	2 cut(s)	2218, 2589
BspTI	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
BsrBI	CCGCTC	1 cut(s)	556
BsrDI	GCAATG	2 cut(s)	583, 1820
BsrI	ACTGG	3 cut(s)	250, 1376, 2393
BssECI	CCNNGG	9 cut(s)	386, 816, 1438, 1619, 2851, 2980, 3089, 3709, 3966
BssSI	CACGAG	1 cut(s)	3276
BssT1I	CCWWGG	6 cut(s)	386, 816, 1438, 2980, 3089, 3966
Bst2BI	CACGAG	1 cut(s)	3276
Bst2UI	CCWGG	4 cut(s)	1332, 1620, 2853, 3710
Bst6I	CTCTTC	4 cut(s)	1187, 2218, 2589, 4041
BstAFI	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
BstC8I	GCNNGC	6 cut(s)	1483, 1879, 1995, 2076, 3326, 3981
BstDSI	CCRYGG	1 cut(s)	1438
BstENI	CCTNNNNNAGG	1 cut(s)	3965
BstHHI	GCGC	1 cut(s)	2114
BstMAI	GTCTC	5 cut(s)	274, 1325, 1914, 1950, 3255
BstMWI	GCNNNNNNNGC	4 cut(s)	311, 1334, 2588, 3222
BstNI	CCWGG	4 cut(s)	1332, 1620, 2853, 3710
BstNSI	RCATGY	3 cut(s)	1066, 3328, 4001
BstSCI	CCNGG	4 cut(s)	1330, 1618, 2851, 3708
BstSFI	CTRYAG	2 cut(s)	1189, 3931
BstSLI	GKGCMC	2 cut(s)	2699, 3600
BstV1I	GCAGC	6 cut(s)	1042, 1147, 3340, 3340, 3514, 3907
BstV2I	GAAGAC	3 cut(s)	2669, 3137, 3406
BstX2I	RGATCY	2 cut(s)	443, 1900
BstYI	RGATCY	2 cut(s)	443, 1900
Bsu36I	CCTNAGG	1 cut(s)	2532
BsuRI	GGCC	3 cut(s)	815, 1562, 3770
BtgI	CCRYGG	1 cut(s)	1438
BtsI	GCAGTG	2 cut(s)	1985, 3598
BtsIMutI	CAGTG	6 cut(s)	373, 736, 1369, 1985, 3033, 3598
BveI	ACCTGC	1 cut(s)	3644
Cac8I	GCNNGC	6 cut(s)	1483, 1879, 1995, 2076, 3326, 3981
CaiI	CAGNNNCTG	1 cut(s)	260
CciI	TCATGA	1 cut(s)	3673
CfoI	GCGC	1 cut(s)	2114
Cfr13I	GGNCC	2 cut(s)	2009, 2825
Csp6I	GTAC	6 cut(s)	243, 2562, 2715, 2739, 3402, 3427
CspCI	CAANNNNNGTGG	2 cut(s)	707, 742
CviQI	GTAC	6 cut(s)	243, 2562, 2715, 2739, 3402, 3427
DraI	TTTAAA	4 cut(s)	862, 1174, 2868, 3607
EaeI	YGGCCR	2 cut(s)	813, 1560
Eam1104I	CTCTTC	4 cut(s)	1187, 2218, 2589, 4041
EarI	CTCTTC	4 cut(s)	1187, 2218, 2589, 4041
Eco130I	CCWWGG	6 cut(s)	386, 816, 1438, 2980, 3089, 3966
Eco32I	GATATC	2 cut(s)	1378, 3958
Eco47I	GGWCC	2 cut(s)	2009, 2825
Eco57I	CTGAAG	5 cut(s)	650, 980, 2502, 2894, 4005
Eco81I	CCTNAGG	1 cut(s)	2532
EcoNI	CCTNNNNNAGG	1 cut(s)	3965
EcoRI	GAATTC	2 cut(s)	835, 4078
EcoRII	CCWGG	4 cut(s)	1330, 1618, 2851, 3708
EcoRV	GATATC	2 cut(s)	1378, 3958
EcoT14I	CCWWGG	6 cut(s)	386, 816, 1438, 2980, 3089, 3966
EcoT22I	ATGCAT	1 cut(s)	3116
ErhI	CCWWGG	6 cut(s)	386, 816, 1438, 2980, 3089, 3966
FalI	AAGNNNNNCTT	4 cut(s)	2571, 2603, 2812, 2844
FaqI	GGGAC	4 cut(s)	92, 1135, 2474, 2951
FauNDI	CATATG	3 cut(s)	3195, 3252, 3474
FbaI	TGATCA	2 cut(s)	646, 763
FblI	GTMKAC	3 cut(s)	340, 638, 2314
Fnu4HI	GCNGC	6 cut(s)	1031, 1161, 3329, 3354, 3528, 3896
Fsp4HI	GCNGC	6 cut(s)	1031, 1161, 3329, 3354, 3528, 3896
GlaI	GCGC	1 cut(s)	2113
GluI	GCNGC	6 cut(s)	1031, 1161, 3329, 3354, 3528, 3896
GsaI	CCCAGC	1 cut(s)	3993
GsuI	CTGGAG	3 cut(s)	45, 1314, 1446
HaeIII	GGCC	3 cut(s)	815, 1562, 3770
HhaI	GCGC	1 cut(s)	2114
Hin6I	GCGC	1 cut(s)	2112
HinP1I	GCGC	1 cut(s)	2112
HincII	GTYRAC	1 cut(s)	484
HindII	GTYRAC	1 cut(s)	484
HindIII	AAGCTT	2 cut(s)	606, 1728
HpyAV	CCTTC	7 cut(s)	955, 1074, 1426, 1584, 2709, 2869, 2875
HpyF10VI	GCNNNNNNNGC	4 cut(s)	311, 1334, 2588, 3222
HspAI	GCGC	1 cut(s)	2112
Ksp22I	TGATCA	2 cut(s)	646, 763
LguI	GCTCTTC	2 cut(s)	2218, 2589
LmnI	GCTCC	7 cut(s)	20, 553, 1333, 2293, 3592, 3623, 3791
Lsp1109I	GCAGC	6 cut(s)	1042, 1147, 3340, 3340, 3514, 3907
LweI	GCATC	5 cut(s)	2509, 2830, 3123, 3865, 4018
MaeIII	GTNAC	3 cut(s)	682, 2145, 3152
MbiI	CCGCTC	1 cut(s)	556
MfeI	CAATTG	4 cut(s)	273, 380, 2509, 3877
MflI	RGATCY	2 cut(s)	443, 1900
MhlI	GDGCHC	3 cut(s)	2384, 2699, 3600
MlsI	TGGCCA	2 cut(s)	815, 1562
MluNI	TGGCCA	2 cut(s)	815, 1562
MlyI	GAGTC	7 cut(s)	114, 1026, 1117, 1327, 1769, 2809, 3679
MmeI	TCCRAC	4 cut(s)	540, 1089, 2885, 3083
Mox20I	TGGCCA	2 cut(s)	815, 1562
Mph1103I	ATGCAT	1 cut(s)	3116
MroXI	GAANNNNTTC	1 cut(s)	707
MscI	TGGCCA	2 cut(s)	815, 1562
MslI	CAYNNNNRTG	3 cut(s)	1683, 3506, 3795
Msp20I	TGGCCA	2 cut(s)	815, 1562
MspCI	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
MspR9I	CCNGG	4 cut(s)	1332, 1620, 2853, 3710
MunI	CAATTG	4 cut(s)	273, 380, 2509, 3877
Mva1269I	GAATGC	3 cut(s)	2253, 3589, 3949
MvaI	CCWGG	4 cut(s)	1332, 1620, 2853, 3710
MwoI	GCNNNNNNNGC	4 cut(s)	311, 1334, 2588, 3222
NcoI	CCATGG	1 cut(s)	1438
NdeI	CATATG	3 cut(s)	3195, 3252, 3474
NheI	GCTAGC	2 cut(s)	1481, 1877
NlaIV	GGNNCC	2 cut(s)	1902, 2909
NmuCI	GTSAC	2 cut(s)	2145, 3152
NsiI	ATGCAT	1 cut(s)	3116
NspI	RCATGY	3 cut(s)	1066, 3328, 4001
PaeI	GCATGC	1 cut(s)	3328
PagI	TCATGA	1 cut(s)	3673
PaqCI	CACCTGC	1 cut(s)	3644
PciI	ACATGT	1 cut(s)	1062
PciSI	GCTCTTC	2 cut(s)	2218, 2589
PcsI	WCGNNNNNNNCGW	1 cut(s)	4080
PctI	GAATGC	3 cut(s)	2253, 3589, 3949
PdmI	GAANNNNTTC	1 cut(s)	707
PkrI	GCNGC	6 cut(s)	1032, 1162, 3330, 3355, 3529, 3897
PleI	GAGTC	7 cut(s)	113, 1025, 1117, 1326, 1768, 2809, 3678
PpsI	GAGTC	7 cut(s)	113, 1025, 1117, 1326, 1768, 2809, 3678
PscI	ACATGT	1 cut(s)	1062
PshBI	ATTAAT	1 cut(s)	1805
PsiI	TTATAA	5 cut(s)	747, 780, 1536, 2369, 3062
Psp6I	CCWGG	4 cut(s)	1330, 1618, 2851, 3708
PspFI	CCCAGC	1 cut(s)	3989
PspGI	CCWGG	4 cut(s)	1330, 1618, 2851, 3708
PspN4I	GGNNCC	2 cut(s)	1902, 2909
PspPI	GGNCC	2 cut(s)	2009, 2825
PstNI	CAGNNNCTG	1 cut(s)	260
PsuI	RGATCY	2 cut(s)	443, 1900
RsaI	GTAC	6 cut(s)	244, 2563, 2716, 2740, 3403, 3428
RsaNI	GTAC	6 cut(s)	243, 2562, 2715, 2739, 3402, 3427
RseI	CAYNNNNRTG	3 cut(s)	1683, 3506, 3795
SapI	GCTCTTC	2 cut(s)	2218, 2589
SatI	GCNGC	6 cut(s)	1031, 1161, 3329, 3354, 3528, 3896
Sau96I	GGNCC	2 cut(s)	2009, 2825
ScaI	AGTACT	3 cut(s)	244, 2563, 3403
SchI	GAGTC	7 cut(s)	114, 1026, 1117, 1327, 1769, 2809, 3679
ScrFI	CCNGG	4 cut(s)	1332, 1620, 2853, 3710
SduI	GDGCHC	3 cut(s)	2384, 2699, 3600
SfaNI	GCATC	5 cut(s)	2509, 2830, 3123, 3865, 4018
SfcI	CTRYAG	2 cut(s)	1189, 3931
SinI	GGWCC	2 cut(s)	2009, 2825
SmiI	ATTTAAAT	1 cut(s)	2868
SmiMI	CAYNNNNRTG	3 cut(s)	1683, 3506, 3795
SmlI	CTYRAG	9 cut(s)	603, 976, 1240, 1488, 1580, 1653, 2613, 2750, 3575
SmoI	CTYRAG	9 cut(s)	603, 976, 1240, 1488, 1580, 1653, 2613, 2750, 3575
SpeI	ACTAGT	1 cut(s)	1829
SphI	GCATGC	1 cut(s)	3328
SsiI	CCGC	6 cut(s)	303, 428, 465, 556, 2057, 3376
StyD4I	CCNGG	4 cut(s)	1330, 1618, 2851, 3708
StyI	CCWWGG	6 cut(s)	386, 816, 1438, 2980, 3089, 3966
SwaI	ATTTAAAT	1 cut(s)	2868
TaqI	TCGA	5 cut(s)	514, 565, 886, 1042, 1758
TatI	WGTACW	4 cut(s)	242, 2561, 2714, 3401
TscAI	CASTG	6 cut(s)	380, 743, 1376, 1985, 3040, 3605
TseFI	GTSAC	2 cut(s)	2145, 3152
TseI	GCWGC	6 cut(s)	1030, 1160, 3328, 3353, 3527, 3895
Tsp45I	GTSAC	2 cut(s)	2145, 3152
TspGWI	ACGGA	1 cut(s)	670
TspRI	CASTG	6 cut(s)	380, 743, 1376, 1985, 3040, 3605
Vha464I	CTTAAG	4 cut(s)	976, 1240, 2750, 3575
VneI	GTGCAC	1 cut(s)	3596
VpaK11BI	GGWCC	2 cut(s)	2009, 2825
VspI	ATTAAT	1 cut(s)	1805
XagI	CCTNNNNNAGG	1 cut(s)	3965
XbaI	TCTAGA	2 cut(s)	1013, 1049
XceI	RCATGY	3 cut(s)	1066, 3328, 4001
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1682, 2515, 2969
XmiI	GTMKAC	3 cut(s)	340, 638, 2314
XmnI	GAANNNNTTC	1 cut(s)	707
ZrmI	AGTACT	3 cut(s)	244, 2563, 3403
Zsp2I	ATGCAT	1 cut(s)	3116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.