Rroxscaffold_2G00130480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017040
Physical Location & Seq
Forward (+)
66624778 .. 66631326
6549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130480.1

Sequence Viewer

Length: 663 bp
ATGCACGCATTGGCTGACAAGCTTGCAAATTATGGTGCTACTAACGTTGTTTCTCCCCCCCCAGCAGCTGCTCTATCTCTCTCGCAACCGCCCCGAACAGAAGAAATCACGATCTCCGGCCACCAAGCAAATCAAGAGACTCAGCTCGCTCAAGCCCTAGTTGACAGCCATGACTTCACCCGGCTTCCGGATCCACCGAAGCGGCTCCCGATTGGTTCCAATCACCGCACAACTCCGATCGGATCTACCATCGGTCCTCGGCTTGCCGAGATGATTGCTGCAGTTATAGAAGTCCGAGTTTGCAGATCGGTCAATGGCTCAAGTTGGAGCTCGATAACACTTAGAAGCTCGATGGCGAAATCCTCCGGTTCGGCCGGCTTCTCCGTCACGATCACGGCTCCGCCTGCCACGTTGGACGGCTCCTCCGAGTTCCGGGCCGACTCGTCCTTGTCAACACAAACCGCAGGTCGGATGGTTTGTGGTTACGAACACGGTGTAGATCGGAAGCAAGGAACGGAGATCGCGGAGAGGAACAACAAGTCGCCGGCGGAGAGAGAGCGCGCCATTGCTTCCTCTCGCTATTTGCTTTCTCTTCCTTTTCTCGCTCTTCCGGCTCGCCGAAACGCTCACCGCTTGGATGGTTTCGAACCGGAGCATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

23.55

Weight (kDa)

8.69

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49330
pyrus_communis pycom15g04920
rosa_chinensis RchiOBHm_Chr3g0473591
rosa_laevigata RLG00000024002
rosa_multiflora Rmu_ssc0000322.1_g000028
rosa_roxburghii Rroxscaffold_2G00130480 Rroxscaffold_3G00255700 Rroxscaffold_6G00408040 Rroxscaffold_7G00194780
rosa_samantha Rh1DG328800 Rh2AG274100 Rh6AG192700 Rh7DG192700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccII CGCG 2 cut(s) 524, 561
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 8 cut(s) 89, 202, 226, 401, 462, 524, 548, 631
AclI AACGTT 1 cut(s) 45
AclWI GGATC 3 cut(s) 185, 198, 250
AcoI YGGCCR 2 cut(s) 118, 372
AfiI CCNNNNNNNGG 3 cut(s) 187, 432, 468
AluBI AGCT 5 cut(s) 22, 68, 145, 330, 348
AluI AGCT 5 cut(s) 22, 68, 145, 330, 348
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 3 cut(s) 185, 198, 250
AlwNI CAGNNNCTG 1 cut(s) 68
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 3 cut(s) 118, 372, 435
ApeKI GCWGC 3 cut(s) 65, 68, 278
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
AspLEI GCGC 2 cut(s) 561, 563
AspS9I GGNCC 2 cut(s) 254, 435
AsuC2I CCSGG 2 cut(s) 181, 434
AsuHPI GGTGA 3 cut(s) 169, 215, 620
AsuII TTCGAA 1 cut(s) 645
AvaII GGWCC 1 cut(s) 254
BamHI GGATCC 1 cut(s) 190
BanII GRGCYC 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 332
BbvI GCAGC 3 cut(s) 55, 77, 265
BccI CCATC 4 cut(s) 257, 346, 466, 632
BceAI ACGGC 2 cut(s) 411, 433
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BcnI CCSGG 2 cut(s) 181, 434
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 4 cut(s) 66, 69, 203, 279
BlsI GCNGC 4 cut(s) 67, 70, 204, 280
Bme1390I CCNGG 2 cut(s) 181, 434
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 2 cut(s) 254, 435
BmiI GGNNCC 5 cut(s) 192, 206, 217, 399, 421
BmrFI CCNGG 2 cut(s) 181, 434
Bpu14I TTCGAA 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 135, 304
BpuMI CCSGG 2 cut(s) 181, 434
BsaJI CCNNGG 1 cut(s) 257
BsaWI WCCGGW 3 cut(s) 187, 365, 649
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 432, 468
Bse118I RCCGGY 2 cut(s) 374, 544
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 187
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 2 cut(s) 477, 643
BseLI CCNNNNNNNGG 3 cut(s) 187, 432, 468
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 155
BsePI GCGCGC 1 cut(s) 559
BseRI GAGGAG 1 cut(s) 412
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 55, 77, 265
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 2 cut(s) 524, 561
Bsh1285I CGRYCG 2 cut(s) 240, 375
BshFI GGCC 3 cut(s) 120, 374, 437
BsiEI CGRYCG 2 cut(s) 240, 375
BsiHKAI GWGCWC 1 cut(s) 332
BsiSI CCGG 9 cut(s) 117, 181, 188, 366, 375, 433, 545, 611, 650
BslI CCNNNNNNNGG 3 cut(s) 187, 432, 468
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 3 cut(s) 120, 374, 437
Bsp119I TTCGAA 1 cut(s) 645
Bsp1286I GDGCHC 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 187
Bsp143I GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
BspACI CCGC 8 cut(s) 89, 202, 226, 401, 462, 524, 548, 631
BspANI GGCC 3 cut(s) 120, 374, 437
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 2 cut(s) 524, 561
BspLI GGNNCC 5 cut(s) 192, 206, 217, 399, 421
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 3 cut(s) 185, 198, 250
BspQI GCTCTTC 1 cut(s) 612
BspT104I TTCGAA 1 cut(s) 645
BsrDI GCAATG 1 cut(s) 564
BsrFI RCCGGY 2 cut(s) 374, 544
BssAI RCCGGY 2 cut(s) 374, 544
BssECI CCNNGG 1 cut(s) 257
BssHII GCGCGC 1 cut(s) 559
BssMI GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 2 cut(s) 597, 612
BstBI TTCGAA 1 cut(s) 645
BstC8I GCNNGC 9 cut(s) 6, 24, 147, 264, 376, 405, 546, 561, 616
BstDEI CTNAG 2 cut(s) 141, 341
BstF5I GGATG 2 cut(s) 477, 643
BstFNI CGCG 2 cut(s) 524, 561
BstHHI GCGC 2 cut(s) 561, 563
BstKTI GATC 8 cut(s) 114, 193, 240, 245, 308, 393, 502, 522
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
BstMCI CGRYCG 2 cut(s) 240, 375
BstMWI GCNNNNNNNGC 2 cut(s) 404, 611
BstSCI CCNGG 2 cut(s) 179, 432
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 2 cut(s) 524, 561
BstV1I GCAGC 3 cut(s) 55, 77, 265
BstX2I RGATCY 2 cut(s) 190, 242
BstYI RGATCY 2 cut(s) 190, 242
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 3 cut(s) 120, 374, 437
BtsCI GGATG 2 cut(s) 477, 643
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 9 cut(s) 6, 24, 147, 264, 376, 405, 546, 561, 616
CaiI CAGNNNCTG 1 cut(s) 68
CfoI GCGC 2 cut(s) 561, 563
Cfr10I RCCGGY 2 cut(s) 374, 544
Cfr13I GGNCC 2 cut(s) 254, 435
CviAII CATG 1 cut(s) 170
DdeI CTNAG 2 cut(s) 141, 341
DpnI GATC 8 cut(s) 113, 192, 239, 244, 307, 392, 501, 521
DpnII GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
EaeI YGGCCR 2 cut(s) 118, 372
EagI CGGCCG 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 597, 612
EarI CTCTTC 2 cut(s) 597, 612
EciI GGCGGA 2 cut(s) 390, 563
Ecl136II GAGCTC 1 cut(s) 330
EclXI CGGCCG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 254
Eco52I CGGCCG 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 332
FaeI CATG 1 cut(s) 173
FaiI YATR 3 cut(s) 33, 171, 287
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 4 cut(s) 66, 69, 203, 279
FokI GGATG 2 cut(s) 484, 650
FriOI GRGCYC 1 cut(s) 332
Fsp4HI GCNGC 4 cut(s) 66, 69, 203, 279
FspBI CTAG 1 cut(s) 158
GlaI GCGC 2 cut(s) 560, 562
GluI GCNGC 4 cut(s) 66, 69, 203, 279
GsaI CCCAGC 1 cut(s) 65
HaeIII GGCC 3 cut(s) 120, 374, 437
HapII CCGG 9 cut(s) 117, 181, 188, 366, 375, 433, 545, 611, 650
HhaI GCGC 2 cut(s) 561, 563
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 2 cut(s) 559, 561
HinP1I GCGC 2 cut(s) 559, 561
HincII GTYRAC 2 cut(s) 163, 453
HindII GTYRAC 2 cut(s) 163, 453
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 139, 440
HpaII CCGG 9 cut(s) 117, 181, 188, 366, 375, 433, 545, 611, 650
HphI GGTGA 3 cut(s) 169, 215, 620
Hpy166II GTNNAC 2 cut(s) 163, 453
Hpy188I TCNGA 6 cut(s) 237, 242, 296, 427, 471, 504
Hpy188III TCNNGA 5 cut(s) 109, 134, 188, 208, 388
Hpy8I GTNNAC 2 cut(s) 163, 453
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 2 cut(s) 45, 410
HpyCH4V TGCA 4 cut(s) 4, 26, 281, 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 611
HpyF3I CTNAG 2 cut(s) 141, 341
HpySE526I ACGT 2 cut(s) 45, 410
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 2 cut(s) 559, 561
Kpn2I TCCGGA 1 cut(s) 187
KroI GCCGGC 2 cut(s) 374, 544
KroNI GCCGGC 2 cut(s) 376, 546
Kzo9I GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 5 cut(s) 210, 327, 403, 425, 652
Lsp1109I GCAGC 3 cut(s) 55, 77, 265
MaeI CTAG 1 cut(s) 158
MaeII ACGT 2 cut(s) 45, 410
MaeIII GTNAC 2 cut(s) 385, 482
MalI GATC 8 cut(s) 113, 192, 239, 244, 307, 392, 501, 521
MboI GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
MboII GAAGA 3 cut(s) 113, 584, 599
MflI RGATCY 2 cut(s) 190, 242
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 1 cut(s) 28
MlyI GAGTC 2 cut(s) 133, 434
MmeI TCCRAC 3 cut(s) 305, 393, 449
MnlI CCTC 5 cut(s) 267, 373, 433, 522, 583
MreI CGCCGGCG 1 cut(s) 544
MroI TCCGGA 1 cut(s) 187
MroNI GCCGGC 2 cut(s) 374, 544
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 9 cut(s) 117, 181, 188, 366, 375, 433, 545, 611, 650
MspR9I CCNGG 2 cut(s) 181, 434
MvnI CGCG 2 cut(s) 524, 561
MwoI GCNNNNNNNGC 2 cut(s) 404, 611
NaeI GCCGGC 2 cut(s) 376, 546
NciI CCSGG 2 cut(s) 181, 434
NdeII GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
NgoMIV GCCGGC 2 cut(s) 374, 544
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 5 cut(s) 192, 206, 217, 399, 421
NmeAIII GCCGAG 2 cut(s) 238, 292
NmuCI GTSAC 1 cut(s) 385
NspV TTCGAA 1 cut(s) 645
PauI GCGCGC 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PdiI GCCGGC 2 cut(s) 376, 546
PkrI GCNGC 4 cut(s) 67, 70, 204, 280
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 2 cut(s) 133, 434
PpsI GAGTC 2 cut(s) 133, 434
Psp124BI GAGCTC 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 61
PspN4I GGNNCC 5 cut(s) 192, 206, 217, 399, 421
PspPI GGNCC 2 cut(s) 254, 435
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 2 cut(s) 190, 242
PteI GCGCGC 1 cut(s) 559
PvuI CGATCG 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 612
SatI GCNGC 4 cut(s) 66, 69, 203, 279
Sau3AI GATC 8 cut(s) 111, 190, 237, 242, 305, 390, 499, 519
Sau96I GGNCC 2 cut(s) 254, 435
SchI GAGTC 2 cut(s) 133, 434
ScrFI CCNGG 2 cut(s) 181, 434
SduI GDGCHC 1 cut(s) 332
SetI ASST 8 cut(s) 24, 48, 70, 147, 332, 350, 413, 469
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 645
SgrAI CRCCGGYG 1 cut(s) 544
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 150, 319
SmoI CTYRAG 2 cut(s) 150, 319
Sse9I AATT 1 cut(s) 28
SsiI CCGC 8 cut(s) 89, 202, 226, 401, 462, 524, 548, 631
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 179, 432
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 2 cut(s) 48, 413
TaqI TCGA 3 cut(s) 332, 350, 645
TaqII GACCGA 2 cut(s) 242, 298
TasI AATT 1 cut(s) 28
TauI GCSGC 1 cut(s) 205
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 65, 68, 278
Tsp45I GTSAC 1 cut(s) 385
TspGWI ACGGA 2 cut(s) 373, 530
VpaK11BI GGWCC 1 cut(s) 254
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.