Rroxscaffold_2G00135170

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006382
Physical Location & Seq
Forward (+)
72800746 .. 72801468
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135170.1

Sequence Viewer

Length: 723 bp
ATGGCTCAAGTGACTCCACTCGGAGCCGGCCTAACTCCAGGCAACAACCCCCACATCATTAGGCCCACTAAGCGCCCCACCATCCTCGTCCCTCTCTGCAGCCTCGTCTTCCTCATCGGCTTCCTCGTCAGCTTCCTGGTTTTGGCCATCTATTTTATGATCACCAGGGATCCCACACTCCCCAAGTTCAAGGTCGAATCGGCCACCGTGACCCAACTCAACATAGAAGGCTCCAAGTTAACCGCCACGTGGGACTTCACTCTCCTTGCCACCAACCCTAGCCGCAAACTTGAACCCTACTACAGCCGTGTCCAGGCCGGGGTCCGCTACCAGGATGGGTGCCGCGATGTTTACACCCTCGTGAAAAACCCGTTCCCTTCGTTTGTTCTGAGCCGGAGGAACCAGACACGTGTCGGTTTGAGACTCGGAGTGTGGAGGCAACCGGGGAGGCAAGTGGCGCAGGGAATCTCCGGCGAGAGGAAGAGTGAGTTGGTGAGTTTCGGAGTTAGACTCCTGGCTAAGGTGTGGTTTGAGTACTCTAGGATATTGCCGGCGGAACGAGGTTGGCTGCTGGTCTCCTGCGATAGGGTTGATTTTGATTTTTCCGGGAAGAATGGGACCGGCGAAATGACGAGTCGGTCTGGGCAGTGCGATGTTGACTACTACCCAATACTTCCCTTGGGTAGTACTATCAGTAAGGATGAGTTGGCAAATGCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

240

Amino Acids

26.79

Weight (kDa)

9.66

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 339
AccII CGCG 1 cut(s) 345
AciI CCGC 5 cut(s) 243, 283, 325, 343, 554
AclWI GGATC 2 cut(s) 164, 177
AcoI YGGCCR 2 cut(s) 144, 201
AcvI CACGTG 2 cut(s) 249, 410
AfaI GTAC 2 cut(s) 536, 688
AfiI CCNNNNNNNGG 8 cut(s) 142, 249, 313, 319, 331, 477, 520, 585
AflIII ACRYGT 2 cut(s) 407, 409
AgsI TTSAA 2 cut(s) 190, 293
AjnI CCWGG 6 cut(s) 37, 135, 164, 312, 330, 513
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 415, 580
AlwI GGATC 2 cut(s) 164, 177
AoxI GGCC 5 cut(s) 28, 62, 144, 201, 315
ApeKI GCWGC 2 cut(s) 99, 568
AspLEI GCGC 2 cut(s) 75, 460
AspS9I GGNCC 3 cut(s) 63, 322, 618
AsuC2I CCSGG 3 cut(s) 319, 444, 607
AsuHPI GGTGA 2 cut(s) 154, 505
AvaII GGWCC 2 cut(s) 322, 618
BalI TGGCCA 1 cut(s) 146
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 339
BauI CACGAG 1 cut(s) 359
BbrPI CACGTG 2 cut(s) 249, 410
BbsI GAAGAC 1 cut(s) 100
BbvI GCAGC 2 cut(s) 111, 555
BccI CCATC 3 cut(s) 89, 155, 329
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 6 cut(s) 39, 137, 166, 314, 332, 515
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 3 cut(s) 319, 444, 607
BcoDI GTCTC 2 cut(s) 415, 580
BfaI CTAG 2 cut(s) 279, 540
BfmI CTRYAG 2 cut(s) 97, 301
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 4 cut(s) 100, 283, 343, 569
BlsI GCNGC 4 cut(s) 101, 284, 344, 570
BmcAI AGTACT 2 cut(s) 536, 688
Bme1390I CCNGG 9 cut(s) 39, 137, 166, 314, 319, 332, 444, 515, 607
Bme18I GGWCC 2 cut(s) 322, 618
BmgT120I GGNCC 3 cut(s) 63, 322, 618
BmiI GGNNCC 7 cut(s) 25, 171, 232, 323, 341, 401, 619
BmrFI CCNGG 9 cut(s) 39, 137, 166, 314, 319, 332, 444, 515, 607
BoxI GACNNNNGTC 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 100
BplI GAGNNNNNCTC 2 cut(s) 495, 527
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 519
BpuMI CCSGG 3 cut(s) 319, 444, 607
BsaAI YACGTR 2 cut(s) 249, 410
BsaI GGTCTC 1 cut(s) 580
BsaJI CCNNGG 4 cut(s) 165, 318, 443, 678
Bsc4I CCNNNNNNNGG 8 cut(s) 142, 249, 313, 319, 331, 477, 520, 585
Bse118I RCCGGY 3 cut(s) 26, 550, 620
BseBI CCWGG 6 cut(s) 39, 137, 166, 314, 332, 515
BseDI CCNNGG 4 cut(s) 165, 318, 443, 678
BseGI GGATG 3 cut(s) 81, 340, 706
BseLI CCNNNNNNNGG 8 cut(s) 142, 249, 313, 319, 331, 477, 520, 585
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 111, 555
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 5 cut(s) 30, 64, 146, 203, 317
BshNI GGYRCC 1 cut(s) 339
BsiSI CCGG 8 cut(s) 27, 318, 394, 443, 471, 551, 606, 621
BslFI GGGAC 3 cut(s) 74, 266, 631
BslI CCNNNNNNNGG 8 cut(s) 142, 249, 313, 319, 331, 477, 520, 585
BsmAI GTCTC 2 cut(s) 415, 580
BsmFI GGGAC 3 cut(s) 74, 266, 631
BsnI GGCC 5 cut(s) 30, 64, 146, 203, 317
Bso31I GGTCTC 1 cut(s) 580
Bsp143I GATC 2 cut(s) 159, 169
BspACI CCGC 5 cut(s) 243, 283, 325, 343, 554
BspANI GGCC 5 cut(s) 30, 64, 146, 203, 317
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 7 cut(s) 25, 171, 232, 323, 341, 401, 619
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 2 cut(s) 164, 177
BspT107I GGYRCC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 580
BsrFI RCCGGY 3 cut(s) 26, 550, 620
BssAI RCCGGY 3 cut(s) 26, 550, 620
BssECI CCNNGG 4 cut(s) 165, 318, 443, 678
BssMI GATC 2 cut(s) 159, 169
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 678
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 6 cut(s) 39, 137, 166, 314, 332, 515
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 476
BstBAI YACGTR 2 cut(s) 249, 410
BstC8I GCNNGC 2 cut(s) 28, 552
BstDEI CTNAG 3 cut(s) 69, 389, 519
BstENI CCTNNNNNAGG 2 cut(s) 518, 583
BstF5I GGATG 3 cut(s) 81, 340, 706
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 460
BstKTI GATC 2 cut(s) 162, 172
BstMAI GTCTC 2 cut(s) 415, 580
BstMBI GATC 2 cut(s) 159, 169
BstMWI GCNNNNNNNGC 2 cut(s) 70, 457
BstNI CCWGG 6 cut(s) 39, 137, 166, 314, 332, 515
BstPAI GACNNNNGTC 1 cut(s) 410
BstSCI CCNGG 9 cut(s) 37, 135, 164, 312, 317, 330, 442, 513, 605
BstSFI CTRYAG 2 cut(s) 97, 301
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 111, 555
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 5 cut(s) 30, 64, 146, 203, 317
BtgZI GCGATG 2 cut(s) 360, 666
BtsCI GGATG 3 cut(s) 81, 340, 706
BtsI GCAGTG 1 cut(s) 653
BtsIMutI CAGTG 1 cut(s) 653
Cac8I GCNNGC 2 cut(s) 28, 552
CfoI GCGC 2 cut(s) 75, 460
Cfr10I RCCGGY 3 cut(s) 26, 550, 620
Cfr13I GGNCC 3 cut(s) 63, 322, 618
Csp6I GTAC 2 cut(s) 535, 687
CviQI GTAC 2 cut(s) 535, 687
DdeI CTNAG 3 cut(s) 69, 389, 519
DpnI GATC 2 cut(s) 161, 171
DpnII GATC 2 cut(s) 159, 169
DrdI GACNNNNNNGTC 1 cut(s) 637
DseDI GACNNNNNNGTC 1 cut(s) 637
EaeI YGGCCR 2 cut(s) 144, 201
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 322, 618
Eco72I CACGTG 2 cut(s) 249, 410
EcoNI CCTNNNNNAGG 2 cut(s) 518, 583
EcoRII CCWGG 6 cut(s) 37, 135, 164, 312, 330, 513
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
FaiI YATR 4 cut(s) 158, 224, 719, 721
FaqI GGGAC 3 cut(s) 74, 266, 631
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 100, 283, 343, 569
FokI GGATG 3 cut(s) 68, 347, 713
Fsp4HI GCNGC 4 cut(s) 100, 283, 343, 569
FspBI CTAG 2 cut(s) 279, 540
GlaI GCGC 2 cut(s) 74, 459
GluI GCNGC 4 cut(s) 100, 283, 343, 569
GsuI CTGGAG 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 5 cut(s) 30, 64, 146, 203, 317
HapII CCGG 8 cut(s) 27, 318, 394, 443, 471, 551, 606, 621
HhaI GCGC 2 cut(s) 75, 460
Hin6I GCGC 2 cut(s) 73, 458
HinP1I GCGC 2 cut(s) 73, 458
HincII GTYRAC 2 cut(s) 240, 658
HindII GTYRAC 2 cut(s) 240, 658
HinfI GANTC 6 cut(s) 13, 197, 423, 465, 510, 634
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 8 cut(s) 27, 318, 394, 443, 471, 551, 606, 621
HphI GGTGA 2 cut(s) 154, 505
Hpy166II GTNNAC 3 cut(s) 240, 352, 658
Hpy188I TCNGA 4 cut(s) 23, 390, 428, 503
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 3 cut(s) 240, 352, 658
HpyAV CCTTC 2 cut(s) 221, 387
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 2 cut(s) 248, 409
HpyCH4V TGCA 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 457
HpyF3I CTNAG 3 cut(s) 69, 389, 519
HpySE526I ACGT 2 cut(s) 248, 409
HspAI GCGC 2 cut(s) 73, 458
KroI GCCGGC 2 cut(s) 26, 550
KroNI GCCGGC 2 cut(s) 28, 552
Ksp22I TGATCA 1 cut(s) 159
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 2 cut(s) 159, 169
LmnI GCTCC 2 cut(s) 23, 236
Lsp1109I GCAGC 2 cut(s) 111, 555
MaeI CTAG 2 cut(s) 279, 540
MaeII ACGT 2 cut(s) 248, 409
MaeIII GTNAC 2 cut(s) 10, 208
MalI GATC 2 cut(s) 161, 171
MboI GATC 2 cut(s) 159, 169
MboII GAAGA 3 cut(s) 100, 493, 622
MflI RGATCY 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 4 cut(s) 7, 417, 504, 643
Mox20I TGGCCA 1 cut(s) 146
MroNI GCCGGC 2 cut(s) 26, 550
MscI TGGCCA 1 cut(s) 146
MseI TTAA 1 cut(s) 239
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 146
MspI CCGG 8 cut(s) 27, 318, 394, 443, 471, 551, 606, 621
MspR9I CCNGG 9 cut(s) 39, 137, 166, 314, 319, 332, 444, 515, 607
MvaI CCWGG 6 cut(s) 39, 137, 166, 314, 332, 515
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 2 cut(s) 70, 457
NaeI GCCGGC 2 cut(s) 28, 552
NciI CCSGG 3 cut(s) 319, 444, 607
NdeII GATC 2 cut(s) 159, 169
NgoMIV GCCGGC 2 cut(s) 26, 550
NlaIV GGNNCC 7 cut(s) 25, 171, 232, 323, 341, 401, 619
NmuCI GTSAC 2 cut(s) 10, 208
OliI CACNNNNGTG 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdiI GCCGGC 2 cut(s) 28, 552
PfeI GAWTC 2 cut(s) 197, 465
PfoI TCCNGGA 1 cut(s) 605
PkrI GCNGC 4 cut(s) 101, 284, 344, 570
PleI GAGTC 4 cut(s) 7, 417, 504, 642
PmaCI CACGTG 2 cut(s) 249, 410
PmlI CACGTG 2 cut(s) 249, 410
PpsI GAGTC 4 cut(s) 7, 417, 504, 642
Ppu21I YACGTR 2 cut(s) 249, 410
PshAI GACNNNNGTC 1 cut(s) 410
Psp6I CCWGG 6 cut(s) 37, 135, 164, 312, 330, 513
PspCI CACGTG 2 cut(s) 249, 410
PspGI CCWGG 6 cut(s) 37, 135, 164, 312, 330, 513
PspN4I GGNNCC 7 cut(s) 25, 171, 232, 323, 341, 401, 619
PspPI GGNCC 3 cut(s) 63, 322, 618
PstI CTGCAG 1 cut(s) 101
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 2 cut(s) 536, 688
RsaNI GTAC 2 cut(s) 535, 687
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 4 cut(s) 100, 283, 343, 569
Sau3AI GATC 2 cut(s) 159, 169
Sau96I GGNCC 3 cut(s) 63, 322, 618
ScaI AGTACT 2 cut(s) 536, 688
SchI GAGTC 4 cut(s) 7, 417, 504, 643
ScrFI CCNGG 9 cut(s) 39, 137, 166, 314, 319, 332, 444, 515, 607
SetI ASST 6 cut(s) 134, 195, 251, 412, 525, 565
SfcI CTRYAG 2 cut(s) 97, 301
SinI GGWCC 2 cut(s) 322, 618
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SsiI CCGC 5 cut(s) 243, 283, 325, 343, 554
SspMI CTAG 2 cut(s) 279, 540
StyD4I CCNGG 9 cut(s) 37, 135, 164, 312, 317, 330, 442, 513, 605
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 2 cut(s) 251, 412
TaqI TCGA 1 cut(s) 195
TaqII GACCGA 1 cut(s) 627
TatI WGTACW 2 cut(s) 534, 686
TauI GCSGC 2 cut(s) 285, 345
TfiI GAWTC 2 cut(s) 197, 465
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 1 cut(s) 653
TseFI GTSAC 2 cut(s) 10, 208
TseI GCWGC 2 cut(s) 99, 568
Tsp45I GTSAC 2 cut(s) 10, 208
TspRI CASTG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 322, 618
XagI CCTNNNNNAGG 2 cut(s) 518, 583
XspI CTAG 2 cut(s) 279, 540
ZrmI AGTACT 2 cut(s) 536, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.