Rroxscaffold_2G00140760

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011198
Physical Location & Seq
Reverse (-)
79057548 .. 79061101
3554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140760.1

Sequence Viewer

Length: 1320 bp
ATGCCAACTGCTATACTACCCAAGGTGAACAGTACTATACATTCTGGTTTCTGGAGAGTGATAAGCCAAAGCGGGATACGAAATTCAAAAAATTTAGTACCTTGTCAAGGGAAATTGTTGTCTCAAAACTCGGGGTTGGCGCGTTCTCTACTGTGTTCGACCCTTTTGGTACCGAACGCTAGTAGATATAGTTTTGGTTCTTTAAACCAGGGAAAATCTATGGCAGCGTCAAGTTCGAAACCTGTGTTTGGAGGAGATCTTTATGTTGATGACTTAATTGCAAGTAGTGGGAATGGATTAGACTTCACAAAACCGGCTGGTGTGTTTTTTAATGATAGAAGTCGTAGCAGCTTCCTGAAAGCTAGCTTGAGTTTGAGAAGAAAAGAATCGTTCAACGGTCGTCTAGTTTCTGTGCATGTTGGACCTTGGTTGAGAAATTTTCATGCCTCGTCTTCCATGTGCTATGCTGCTGGTGCTGCTCAGAATGTGTCAGTTGATGGCAACTCCAATGAAGAACAGCTTGCAAATTCTACTGTTTTGTCTGATCAACCTGTTTTGGGAGAGAGAACTCTGAAGCTACTTTCAGGATCATGTTACTTGCCACATCCTGAGAAGGAGGAAACAGGAGGAGAGGATGCCCACTTCATTTGTGAAGATGCACAGGCAATTGGTGTAGCAGATGGTGTGGGTGGATGGGCTGATGTTGGTGTTAATGCCGGACTATTTGCACGGGAACTTATGTCTCATTCTGTTAAGGCAATTCAAGAGGAGCCTAAGGGTAGCTTTGAACCCGCCAGGGTGTTGGAGAAGGCCCACTCATGCACCAAAGCAAAGGGTTCTTCAACAGCTTGCATCATTGGCCTTACTGAGAAGGGCTTACATGCTATCAATCTTGGGGACAGTGGATTCGTAGTGGTTAGAGATGGAAGCACCATCTTCCGATCCCCTGTTCAGCAGCATGGCTTCAATTTTACATATCAGCTGGAAAGTGGCAGTGGGGCTGATCTACCAAGCTCCGGTCAGGTATTTTTGATACCTGTCGCTGCAGGGGATGTCATTATTGCTGGGACGGATGGGTTGTTTGACAATTTGTATAATAGTGAGGTTACTGCTGTTGTTGTTCATGCCGTGAGAACTGGCTTGGAACCACAAGCAACTGCCCAGAAAATAGCAGCTTTAGCACGCCAGCGAGCACTAGATAAGAACAGGCAAACACCATTTTCCACTGCTGCTCAAGATGCAGGGTTCCGTTATTATGGAGGCAAGCTTGATGATGTCACCGTTGTTGTGTCCTACATAACTAGCTCCATCAAGGTGTGA

Protein Analysis

439

Amino Acids

46.49

Weight (kDa)

7.64

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 211 - 390 6.5e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66720 AT5G66720
fragaria_vesca FvH4_1g13720 FvH4_1g13720
malus_domestica MD02G1151900.v1.1 MD15G1266300.v1.1
prunus_persica Prupe.7G151100_v2.0.a1
pyrus_communis pycom02g11930 pycom15g23180
rosa_chinensis RchiOBHm_Chr2g0102131
rosa_laevigata RLG00000017132
rosa_multiflora Rmu_co8315037.1_g000001 Rmu_sc0000157.1_g000039
rosa_roxburghii Rroxscaffold_2G00140760
rosa_rugosa Rorug02G0104600
rosa_samantha Rh2AG153000 Rh2BG158900 Rh2CG158200 Rh2DG158600
rosa_wichuraiana Rw2G011940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 169
AccII CGCG 1 cut(s) 142
AciI CCGC 2 cut(s) 72, 792
AclWI GGATC 2 cut(s) 595, 936
AcsI RAATTY 4 cut(s) 82, 91, 436, 526
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 3 cut(s) 34, 99, 171
AfiI CCNNNNNNNGG 4 cut(s) 107, 248, 557, 1016
AgsI TTSAA 6 cut(s) 87, 394, 764, 788, 843, 967
AjnI CCWGG 2 cut(s) 207, 794
Alw21I GWGCWC 1 cut(s) 1195
Alw26I GTCTC 2 cut(s) 126, 747
AlwI GGATC 2 cut(s) 595, 936
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 810, 859
ApeKI GCWGC 8 cut(s) 224, 348, 467, 476, 955, 1043, 1172, 1229
ApoI RAATTY 4 cut(s) 82, 91, 436, 526
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
Asp718I GGTACC 1 cut(s) 169
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 2 cut(s) 422, 811
AsuHPI GGTGA 2 cut(s) 37, 1270
AsuII TTCGAA 1 cut(s) 236
AsuNHI GCTAGC 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 1 cut(s) 422
AxyI CCTNAGG 1 cut(s) 774
BanI GGYRCC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 1195
BbvI GCAGC 8 cut(s) 236, 360, 454, 463, 967, 1030, 1184, 1216
BccI CCATC 7 cut(s) 491, 674, 687, 917, 941, 1067, 1316
BceAI ACGGC 1 cut(s) 1112
BciT130I CCWGG 2 cut(s) 209, 796
BciVI GTATCC 1 cut(s) 69
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 126, 747
BfaI CTAG 5 cut(s) 180, 363, 404, 1196, 1302
BfmI CTRYAG 1 cut(s) 1044
BfuI GTATCC 1 cut(s) 69
BglII AGATCT 1 cut(s) 256
BisI GCNGC 8 cut(s) 225, 349, 468, 477, 956, 1044, 1173, 1230
BlsI GCNGC 8 cut(s) 226, 350, 469, 478, 957, 1045, 1174, 1231
BmcAI AGTACT 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 209, 796
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 422, 811
BmiI GGNNCC 4 cut(s) 171, 771, 1146, 1247
BmrFI CCNGG 2 cut(s) 209, 796
BmsI GCATC 4 cut(s) 625, 646, 861, 1228
BmtI GCTAGC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 73
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 2 cut(s) 388, 1218
BsaJI CCNNGG 4 cut(s) 21, 208, 425, 795
BsaWI WCCGGW 1 cut(s) 1016
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 248, 557, 1016
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 1141
Bse21I CCTNAGG 1 cut(s) 774
BseBI CCWGG 2 cut(s) 209, 796
BseDI CCNNGG 4 cut(s) 21, 208, 425, 795
BseGI GGATG 5 cut(s) 604, 640, 698, 1057, 1078
BseLI CCNNNNNNNGG 4 cut(s) 107, 248, 557, 1016
BseMII CTCAG 3 cut(s) 494, 600, 858
BseNI ACTGG 1 cut(s) 1141
BseRI GAGGAG 3 cut(s) 267, 642, 782
BseXI GCAGC 8 cut(s) 236, 360, 454, 463, 967, 1030, 1184, 1216
BseYI CCCAGC 1 cut(s) 1064
Bsh1236I CGCG 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 2 cut(s) 812, 861
BshNI GGYRCC 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 1195
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 3 cut(s) 314, 717, 1017
BslFI GGGAC 2 cut(s) 911, 1081
BslI CCNNNNNNNGG 4 cut(s) 107, 248, 557, 1016
BsmAI GTCTC 2 cut(s) 126, 747
BsmFI GGGAC 2 cut(s) 911, 1081
BsnI GGCC 2 cut(s) 812, 861
BsoBI CYCGRG 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 1195
Bsp143I GATC 5 cut(s) 256, 544, 587, 941, 1003
BspACI CCGC 2 cut(s) 72, 792
BspANI GGCC 2 cut(s) 812, 861
BspCNI CTCAG 3 cut(s) 493, 601, 859
BspFNI CGCG 1 cut(s) 142
BspLI GGNNCC 4 cut(s) 171, 771, 1146, 1247
BspMAI CTGCAG 1 cut(s) 1048
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 2 cut(s) 595, 936
BspT104I TTCGAA 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 1 cut(s) 1141
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 4 cut(s) 21, 208, 425, 795
BssMI GATC 5 cut(s) 256, 544, 587, 941, 1003
BssT1I CCWWGG 2 cut(s) 21, 425
Bst2UI CCWGG 2 cut(s) 209, 796
Bst4CI ACNGT 6 cut(s) 32, 153, 398, 535, 902, 1282
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 7 cut(s) 364, 522, 850, 1183, 1187, 1191, 1265
BstDEI CTNAG 4 cut(s) 480, 609, 774, 867
BstENI CCTNNNNNAGG 1 cut(s) 105
BstF5I GGATG 5 cut(s) 604, 640, 698, 1057, 1078
BstFNI CGCG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 5 cut(s) 259, 547, 590, 944, 1006
BstMAI GTCTC 2 cut(s) 126, 747
BstMBI GATC 5 cut(s) 256, 544, 587, 941, 1003
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 5 cut(s) 473, 476, 858, 1178, 1238
BstNI CCWGG 2 cut(s) 209, 796
BstNSI RCATGY 2 cut(s) 419, 884
BstSCI CCNGG 2 cut(s) 207, 794
BstSFI CTRYAG 1 cut(s) 1044
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 8 cut(s) 236, 360, 454, 463, 967, 1030, 1184, 1216
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 802
BstYI RGATCY 1 cut(s) 256
Bsu36I CCTNAGG 1 cut(s) 774
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 812, 861
BtsCI GGATG 5 cut(s) 604, 640, 698, 1057, 1078
BtsI GCAGTG 2 cut(s) 1000, 1224
BtsIMutI CAGTG 3 cut(s) 907, 1000, 1224
Cac8I GCNNGC 7 cut(s) 364, 522, 850, 1183, 1187, 1191, 1265
CfoI GCGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 422, 811
CseI GACGC 1 cut(s) 216
Csp6I GTAC 3 cut(s) 33, 98, 170
CspCI CAANNNNNGTGG 2 cut(s) 629, 664
CviAII CATG 8 cut(s) 416, 443, 457, 591, 819, 881, 959, 1124
CviQI GTAC 3 cut(s) 33, 98, 170
DdeI CTNAG 4 cut(s) 480, 609, 774, 867
DpnI GATC 5 cut(s) 258, 546, 589, 943, 1005
DpnII GATC 5 cut(s) 256, 544, 587, 941, 1003
DraI TTTAAA 1 cut(s) 204
Eco130I CCWWGG 2 cut(s) 21, 425
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 207, 794
EcoT14I CCWWGG 2 cut(s) 21, 425
ErhI CCWWGG 2 cut(s) 21, 425
FaeI CATG 8 cut(s) 419, 446, 460, 594, 822, 884, 962, 1127
FalI AAGNNNNNCTT 4 cut(s) 504, 536, 767, 799
FaqI GGGAC 2 cut(s) 911, 1081
FatI CATG 8 cut(s) 415, 442, 456, 590, 818, 880, 958, 1123
FauI CCCGC 2 cut(s) 65, 799
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 8 cut(s) 225, 349, 468, 477, 956, 1044, 1173, 1230
FokI GGATG 5 cut(s) 591, 647, 705, 1064, 1085
Fsp4HI GCNGC 8 cut(s) 225, 349, 468, 477, 956, 1044, 1173, 1230
FspBI CTAG 5 cut(s) 180, 363, 404, 1196, 1302
GlaI GCGC 1 cut(s) 141
GluI GCNGC 8 cut(s) 225, 349, 468, 477, 956, 1044, 1173, 1230
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 2 cut(s) 812, 861
HapII CCGG 3 cut(s) 314, 717, 1017
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 142
Hin1II CATG 8 cut(s) 419, 446, 460, 594, 822, 884, 962, 1127
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 2 cut(s) 386, 906
HpaII CCGG 3 cut(s) 314, 717, 1017
HphI GGTGA 2 cut(s) 37, 1270
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 483, 544, 573, 941
Hpy188III TCNNGA 6 cut(s) 52, 355, 585, 608, 764, 1235
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 607, 802, 865
HpyCH4III ACNGT 6 cut(s) 32, 153, 398, 535, 902, 1282
HpyCH4V TGCA 9 cut(s) 281, 415, 524, 659, 728, 822, 852, 1046, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 473, 476, 858, 1178, 1238
HpyF3I CTNAG 4 cut(s) 480, 609, 774, 867
Hsp92II CATG 8 cut(s) 419, 446, 460, 594, 822, 884, 962, 1127
HspAI GCGC 1 cut(s) 140
KpnI GGTACC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 5 cut(s) 256, 544, 587, 941, 1003
LmnI GCTCC 3 cut(s) 769, 1019, 1310
Lsp1109I GCAGC 8 cut(s) 236, 360, 454, 463, 967, 1030, 1184, 1216
LweI GCATC 4 cut(s) 625, 646, 861, 1228
MaeI CTAG 5 cut(s) 180, 363, 404, 1196, 1302
MaeIII GTNAC 3 cut(s) 593, 1105, 1276
MalI GATC 5 cut(s) 258, 546, 589, 943, 1005
MboI GATC 5 cut(s) 256, 544, 587, 941, 1003
MboII GAAGA 6 cut(s) 390, 444, 524, 665, 831, 928
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 1195
MmeI TCCRAC 2 cut(s) 400, 783
MnlI CCTC 8 cut(s) 245, 457, 610, 620, 625, 760, 1096, 1253
MseI TTAA 5 cut(s) 203, 275, 330, 711, 753
MslI CAYNNNNRTG 1 cut(s) 1313
MspA1I CMGCKG 1 cut(s) 982
MspI CCGG 3 cut(s) 314, 717, 1017
MspR9I CCNGG 2 cut(s) 209, 796
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 2 cut(s) 209, 796
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 5 cut(s) 473, 476, 858, 1178, 1238
NdeII GATC 5 cut(s) 256, 544, 587, 941, 1003
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 8 cut(s) 419, 446, 460, 594, 822, 884, 962, 1127
NlaIV GGNNCC 4 cut(s) 171, 771, 1146, 1247
NmuCI GTSAC 1 cut(s) 1276
NspI RCATGY 2 cut(s) 419, 884
NspV TTCGAA 1 cut(s) 236
PfeI GAWTC 2 cut(s) 386, 906
PkrI GCNGC 8 cut(s) 226, 350, 469, 478, 957, 1045, 1174, 1231
Psp6I CCWGG 2 cut(s) 207, 794
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 2 cut(s) 207, 794
PspN4I GGNNCC 4 cut(s) 171, 771, 1146, 1247
PspPI GGNCC 2 cut(s) 422, 811
PstI CTGCAG 1 cut(s) 1048
PsuI RGATCY 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 982
RsaI GTAC 3 cut(s) 34, 99, 171
RsaNI GTAC 3 cut(s) 33, 98, 170
RseI CAYNNNNRTG 1 cut(s) 1313
SaqAI TTAA 5 cut(s) 203, 275, 330, 711, 753
SatI GCNGC 8 cut(s) 225, 349, 468, 477, 956, 1044, 1173, 1230
Sau3AI GATC 5 cut(s) 256, 544, 587, 941, 1003
Sau96I GGNCC 2 cut(s) 422, 811
ScaI AGTACT 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 209, 796
SduI GDGCHC 1 cut(s) 1195
SfaNI GCATC 4 cut(s) 625, 646, 861, 1228
SfcI CTRYAG 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 236
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 2 cut(s) 367, 1233
SmoI CTYRAG 2 cut(s) 367, 1233
SsiI CCGC 2 cut(s) 72, 792
SspMI CTAG 5 cut(s) 180, 363, 404, 1196, 1302
StyD4I CCNGG 2 cut(s) 207, 794
StyI CCWWGG 2 cut(s) 21, 425
TaaI ACNGT 6 cut(s) 32, 153, 398, 535, 902, 1282
TaqI TCGA 2 cut(s) 158, 236
TatI WGTACW 1 cut(s) 32
TfiI GAWTC 2 cut(s) 386, 906
Tru1I TTAA 5 cut(s) 203, 275, 330, 711, 753
Tru9I TTAA 5 cut(s) 203, 275, 330, 711, 753
TscAI CASTG 3 cut(s) 907, 1000, 1231
TseFI GTSAC 1 cut(s) 1276
TseI GCWGC 8 cut(s) 224, 348, 467, 476, 955, 1043, 1172, 1229
Tsp45I GTSAC 1 cut(s) 1276
TspDTI ATGAA 4 cut(s) 431, 525, 634, 1112
TspGWI ACGGA 2 cut(s) 1085, 1238
TspRI CASTG 3 cut(s) 907, 1000, 1231
VpaK11BI GGWCC 1 cut(s) 422
XagI CCTNNNNNAGG 1 cut(s) 105
XapI RAATTY 4 cut(s) 82, 91, 436, 526
XceI RCATGY 2 cut(s) 419, 884
XspI CTAG 5 cut(s) 180, 363, 404, 1196, 1302
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.