Rroxscaffold_3G00260170
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003306
Physical Location & Seq
Forward (+)
54560763 .. 54583696
22934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260170.1

Sequence Viewer

Length: 645 bp
ATGGAGTTCGTGCGAGAGAGTCCTCACCGGCTTTTCAAGAGAGGAAGAGCCATTGAAGCCGAAGCTGAATTTGAGAAACTTTTAGGAGGAGCACATGTTAAATTTGCAATGGTAGAATTGTCAAAGTCTGAAAGAGGGGATGAATTGGAGACAAGTAAAGTTCTCGAGCTACTATATGGCCGCCATTTCAAAATGGTTTTCATTAGCATAAATGCTATATTCTATTTCTCCTCAACGGTCTTTAAAAGCTTCGGTGTGCCTTCGGATATTGCAAATGTATGTGTCGGAGTTGCAAATCTATCGGGGTCCATTGTTGCAATGATATTGATGGATAAGCTTGGAAGAAGGGTGCTTCTTCTTGGAAGTTTTACCGGCATGGCAGTGTCAATGAGTCTTCAAGTAAGTGGAGCAAGTGTTATGGAGCAAAGTAGAAGAAAGGAAGCTAAATCGGCGCGTTCGAAGAAGCGACTGTTCACTCCAAAGAAAGAAAAAATCCCTTCCGACAAATACACTAAGCACCTCTTGGAAGATGCCAATTACGAAAGATTTCTGAAGAAAATGAAAATGGTATTGGTAAAGAGTATTAGTGCGTTAGCGGATAATCCTGCAGGTCGGGTGAACCAAGGTGGTGATCGAGCTGGTTAG

Protein Analysis

214

Amino Acids

23.8

Weight (kDa)

9.9

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 3 - 133 8.6e-18 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 599
AccII CGCG 1 cut(s) 454
AciI CCGC 2 cut(s) 181, 596
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 2 cut(s) 68, 101
AcuI CTGAAG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 4 cut(s) 37, 56, 190, 398
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 6 cut(s) 65, 169, 249, 337, 443, 638
AluI AGCT 6 cut(s) 65, 169, 249, 337, 443, 638
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 1 cut(s) 178
ApoI RAATTY 2 cut(s) 68, 101
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 17, 628, 641
AsuII TTCGAA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 94
BccI CCATC 1 cut(s) 322
BcgI CGANNNNNNTGC 2 cut(s) 282, 316
BcoDI GTCTC 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 606
BfuAI ACCTGC 1 cut(s) 599
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 1 cut(s) 307
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 622
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 399, 429
Bse118I RCCGGY 2 cut(s) 27, 371
Bse3DI GCAATG 2 cut(s) 114, 324
BseDI CCNNGG 1 cut(s) 622
BseGI GGATG 1 cut(s) 145
BseMI GCAATG 2 cut(s) 114, 324
BseRI GAGGAG 2 cut(s) 102, 220
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 28, 372
BsmAI GTCTC 1 cut(s) 143
BsnI GGCC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 1 cut(s) 631
BspACI CCGC 2 cut(s) 181, 596
BspANI GGCC 1 cut(s) 180
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 307
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 599
BspQI GCTCTTC 1 cut(s) 40
BspT104I TTCGAA 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 114, 324
BsrFI RCCGGY 2 cut(s) 27, 371
BssAI RCCGGY 2 cut(s) 27, 371
BssECI CCNNGG 1 cut(s) 622
BssMI GATC 1 cut(s) 631
BssT1I CCWWGG 1 cut(s) 622
Bst4CI ACNGT 2 cut(s) 238, 471
Bst6I CTCTTC 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 458
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 1 cut(s) 634
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 1 cut(s) 631
BstMWI GCNNNNNNNGC 2 cut(s) 56, 449
BstNSI RCATGY 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 454
BstV2I GAAGAC 1 cut(s) 386
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 599
CfoI GCGC 1 cut(s) 454
Cfr10I RCCGGY 2 cut(s) 27, 371
Cfr13I GGNCC 1 cut(s) 306
CviAII CATG 2 cut(s) 95, 376
DdeI CTNAG 1 cut(s) 513
DpnI GATC 1 cut(s) 633
DpnII GATC 1 cut(s) 631
DraI TTTAAA 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 2 cut(s) 98, 379
FaiI YATR 8 cut(s) 96, 175, 177, 209, 218, 280, 377, 419
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FatI CATG 2 cut(s) 94, 375
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 181
GlaI GCGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 180
HapII CCGG 2 cut(s) 28, 372
HhaI GCGC 1 cut(s) 454
Hin1II CATG 2 cut(s) 98, 379
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HindIII AAGCTT 2 cut(s) 247, 335
HinfI GANTC 2 cut(s) 19, 391
HpaII CCGG 2 cut(s) 28, 372
HphI GGTGA 3 cut(s) 17, 628, 641
Hpy166II GTNNAC 2 cut(s) 474, 619
Hpy188I TCNGA 5 cut(s) 130, 265, 287, 502, 552
Hpy188III TCNNGA 2 cut(s) 37, 164
Hpy8I GTNNAC 2 cut(s) 474, 619
HpyAV CCTTC 3 cut(s) 270, 339, 507
HpyCH4III ACNGT 2 cut(s) 238, 471
HpyCH4V TGCA 5 cut(s) 107, 272, 293, 317, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 449
HpyF3I CTNAG 1 cut(s) 513
Hsp92II CATG 2 cut(s) 98, 379
HspAI GCGC 1 cut(s) 452
Kzo9I GATC 1 cut(s) 631
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 89, 407, 421
LpnPI CCDG 5 cut(s) 41, 385, 594, 618, 624
LweI GCATC 1 cut(s) 520
MalI GATC 1 cut(s) 633
MboI GATC 1 cut(s) 631
MboII GAAGA 8 cut(s) 57, 347, 354, 386, 444, 472, 539, 565
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 5 cut(s) 68, 101, 116, 143, 535
MlyI GAGTC 2 cut(s) 28, 400
MmeI TCCRAC 2 cut(s) 265, 525
MnlI CCTC 6 cut(s) 33, 35, 80, 128, 241, 530
MroXI GAANNNNTTC 1 cut(s) 546
MseI TTAA 2 cut(s) 99, 243
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 2 cut(s) 28, 372
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 2 cut(s) 56, 449
NdeII GATC 1 cut(s) 631
NlaIII CATG 2 cut(s) 98, 379
NlaIV GGNNCC 1 cut(s) 307
NspI RCATGY 1 cut(s) 98
NspV TTCGAA 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 164
PciI ACATGT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 546
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 2 cut(s) 27, 399
PpsI GAGTC 2 cut(s) 27, 399
PscI ACATGT 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 306
PstI CTGCAG 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 380
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 2 cut(s) 99, 243
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 1 cut(s) 631
Sau96I GGNCC 1 cut(s) 306
SbfI CCTGCAGG 1 cut(s) 610
SchI GAGTC 2 cut(s) 28, 400
SdaI CCTGCAGG 1 cut(s) 610
SduI GDGCHC 1 cut(s) 94
SetI ASST 9 cut(s) 67, 171, 251, 339, 445, 522, 613, 628, 640
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 1 cut(s) 606
Sfr274I CTCGAG 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse8387I CCTGCAGG 1 cut(s) 610
Sse9I AATT 5 cut(s) 68, 101, 116, 143, 535
SsiI CCGC 2 cut(s) 181, 596
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 2 cut(s) 238, 471
TaqI TCGA 3 cut(s) 165, 458, 634
TasI AATT 5 cut(s) 68, 101, 116, 143, 535
TauI GCSGC 1 cut(s) 183
Tru1I TTAA 2 cut(s) 99, 243
Tru9I TTAA 2 cut(s) 99, 243
TscAI CASTG 1 cut(s) 387
TspDTI ATGAA 3 cut(s) 156, 190, 575
TspRI CASTG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 306
XapI RAATTY 2 cut(s) 68, 101
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.