Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0006183

Physical Location & Seq

Genomic Coordinates Forward (+)

24948598 .. 24955831

Gene Structure 7234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00304290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGAGTTTCCTCAAAGGCCTCATCGACTCTTTAGGCTCCGTCTTCTCCGAATCCTCCTCCTCCTCCTCCTCATACCCAAACCCTACCGCTTCCAATTCCTCCGCCATGGACGGCGTAGCTGGGCCCGGAGCTGCTTCGGTCACCAACGAGCGCGTGGCGTACAAGCTCAAGGGGTACTTTGATTTGGCCAAGGAAGAGATCGCCAAGGCTGTTAGGGCTGAAGAATGGGGCTTGGTTGAAGACGCCGTTGCACACTATAATAGCGCCCAGAGAATTCTAGCCGAAGCTAGCTCCACTCCAGTGCCTTCCTTTATCAGCGCCAGTGAGCGAGAGAAGGTGAAATCGTATCGTCAAAAGATATCAAAATGGCAGGGTCAAGTTTCTGATAGGTTACAAGTCTTAAGTAGACGTCCAGGTGGCACGTCCATAAGCAAGACCGTAGCTCCTGCACGAACTACTGCAGCTACATCAACAACGTTGAATTCACGAAATCATGTTATACCCAAGACTCTGCATCCCAGTACAAGCATGCCAGCAATGAGGAGTCAGACCAATAATATTGTAAGATCAAAACCTGTGCAAGATTCTGGTGCAGGTTATGATGAAAAATTGGTTGAAATGATAAATAGTGCCATAGTGGATAGAAGTCCTTCTGTTAAATGGGAAGATGTTGCTGGTCTTGAGAAGGTGAAGAAAACTTTGATGGAGATGGTTATTTTACCAACTAAGAGAAGAGATTTGTTCACTGGCCTTCGAAGGCCAGCTCGAGGTCTGCTTCTGTTTGGTCCACCTGGTAATGGGAAAACCATGCTTGCTAAGGCAGTTGCTTCAGAATCAGAGGCAACCTTTTTTAATGTATCTGCATCTTCACTGACATCAAAATGGGTGGGAGAGGCTGAAAAGCTTGTGCGGACACTTTTCATGGTTGCTATATCCAGACAGCCATCTGTAATTTTCATGGATGAGATTGACAGTATCATGTCAACAAGGCAGGCAAACGAAAATGATGCAAGCAGAAGGTTGAAGTCAGAGTTTCTAATTCAGTTTGATGGAGTGACATCTAATCCTAATGATTTAGTAATTGTGATTGGTGCTACTAATAAGCCTCAAGAACTAGATGACGCAGTTCTTAGGAGATTGGTGAAGAGAGTATACATACCTTTACCAGATCTACCTGCTAGGAGACTTCTCCTAAGTCACAAACTCAAAGGCCAAGCATTTTCCTTACCAAGTGGAGATCTTGAACGACTTGCAAGAGACACTGAAGGTTATTCAGGAAGTGATCTACAAGCATTGTGTGAAGAAGCAGCAATGATGCCAATTAGGGAACTTGGTGAAAACATTCTCACGATCAGGGCAAATCAGGTAAGACCACTAAGAATTGAAGATTTTCAGAAGGCAATGACTGTAATCAGGCCAAGTTTAAGCAAAAGCAAGTGGGAAGAGCTTGAGCAATGGAACGAGGAATTTGGGTCTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0001578 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0006810 GO:0006928 GO:0006996 GO:0006997 GO:0006998 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008017 GO:0008088 GO:0008089 GO:0008092 GO:0008150 GO:0008568 GO:0009987 GO:0010256 GO:0010458 GO:0010970 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019896 GO:0022402 GO:0022411 GO:0022607 GO:0030496 GO:0030705 GO:0031090 GO:0031468 GO:0031965 GO:0031967 GO:0031975 GO:0034214 GO:0034643 GO:0042623 GO:0043014 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0046907 GO:0047497 GO:0048285 GO:0048487 GO:0051013 GO:0051179 GO:0051228 GO:0051230 GO:0051234 GO:0051259 GO:0051260 GO:0051301 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0061024 GO:0061640 GO:0065003 GO:0071840 GO:0072384 GO:0090148 GO:0098930 GO:0099111 GO:0140014 GO:0140096 GO:1902850 GO:1903008 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Vps4_C

Protein Analysis

493

Amino Acids

54.18

Weight (kDa)

9.3

Isoelectric Point (pI)

51.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	258 - 388	4.1e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	413 - 447	1e-13	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45500 AT2G45500 AT2G45500 AT2G45500 AT2G45500
fragaria_vesca	FvH4_7g12180 FvH4_7g12180
malus_domestica	MD02G1200800.v1.1
prunus_persica	Prupe.2G148300_v2.0.a1 Prupe.2G148300_v2.0.a1 Prupe.2G148300_v2.0.a1
pyrus_communis	pycom02g16400
rosa_chinensis	RchiOBHm_Chr1g0350931
rosa_laevigata	RLG00000028440
rosa_multiflora	Rmu_sc0002665.1_g000019 Rmu_sc0006458.1_g000018
rosa_roxburghii	Rroxscaffold_4G00304290
rosa_rugosa	Rorug01G0075600 Rorug01G0215400 Rorug01G0215400 Rorug06G0023300
rosa_samantha	Rh1AG230100 Rh1BG075200 Rh1BG200000 Rh1CG214500 Rh1DG227800
rosa_wichuraiana	Rw1G019950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	412
Acc36I	ACCTGC	2 cut(s)	584, 1183
AccI	GTMKAC	2 cut(s)	406, 1152
AccII	CGCG	1 cut(s)	153
AciI	CCGC	3 cut(s)	87, 102, 910
AclI	AACGTT	1 cut(s)	476
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	3 cut(s)	273, 481, 1466
AcuI	CTGAAG	3 cut(s)	240, 813, 1284
AcyI	GRCGYC	2 cut(s)	243, 409
AfaI	GTAC	3 cut(s)	161, 176, 523
AfiI	CCNNNNNNNGG	2 cut(s)	767, 797
AflII	CTTAAG	1 cut(s)	400
AgsI	TTSAA	6 cut(s)	239, 481, 617, 1024, 1244, 1385
AjiI	CACGTC	1 cut(s)	423
AjnI	CCWGG	2 cut(s)	412, 790
AleI	CACNNNNGTG	1 cut(s)	299
Alw26I	GTCTC	2 cut(s)	1177, 1251
Ama87I	CYCGRG	1 cut(s)	765
AoxI	GGCC	7 cut(s)	16, 122, 186, 748, 758, 1210, 1415
ApaI	GGGCCC	1 cut(s)	126
ApeKI	GCWGC	3 cut(s)	131, 461, 1307
ApoI	RAATTY	3 cut(s)	273, 481, 1466
Asp700I	GAANNNNTTC	3 cut(s)	649, 1341, 1389
AspLEI	GCGC	3 cut(s)	153, 266, 320
AspS9I	GGNCC	3 cut(s)	122, 123, 785
AsuC2I	CCSGG	1 cut(s)	126
AsuHPI	GGTGA	5 cut(s)	133, 349, 700, 1153, 1346
AsuII	TTCGAA	1 cut(s)	754
AsuNHI	GCTAGC	1 cut(s)	287
AvaI	CYCGRG	1 cut(s)	765
AvaII	GGWCC	1 cut(s)	785
BaeGI	GKGCMC	1 cut(s)	126
BalI	TGGCCA	1 cut(s)	188
BanII	GRGCYC	1 cut(s)	126
BarI	GAAGNNNNNNTAC	2 cut(s)	1136, 1168
BbsI	GAAGAC	2 cut(s)	34, 246
BbvI	GCAGC	3 cut(s)	118, 473, 1319
BccI	CCATC	4 cut(s)	697, 703, 952, 1043
BceAI	ACGGC	2 cut(s)	127, 230
BcgI	CGANNNNNNTGC	2 cut(s)	989, 1023
BciT130I	CCWGG	2 cut(s)	414, 792
BcnI	CCSGG	1 cut(s)	126
BcoDI	GTCTC	2 cut(s)	1177, 1251
BfaI	CTAG	4 cut(s)	278, 288, 1115, 1179
BfmI	CTRYAG	1 cut(s)	459
BfoI	RGCGCY	2 cut(s)	267, 321
BfrI	CTTAAG	1 cut(s)	400
BfuAI	ACCTGC	2 cut(s)	584, 1183
BglII	AGATCT	2 cut(s)	1168, 1237
BisI	GCNGC	3 cut(s)	132, 462, 1308
BlsI	GCNGC	3 cut(s)	133, 463, 1309
Bme1390I	CCNGG	3 cut(s)	126, 414, 792
Bme18I	GGWCC	1 cut(s)	785
BmeT110I	CYCGRG	1 cut(s)	765
BmgBI	CACGTC	1 cut(s)	423
BmgT120I	GGNCC	3 cut(s)	122, 123, 785
BmiI	GGNNCC	2 cut(s)	37, 124
BmrFI	CCNGG	3 cut(s)	126, 414, 792
BmrI	ACTGGG	1 cut(s)	513
BmsI	GCATC	4 cut(s)	523, 872, 997, 1305
BmtI	GCTAGC	1 cut(s)	291
BmuI	ACTGGG	1 cut(s)	513
BpiI	GAAGAC	2 cut(s)	34, 246
BpmI	CTGGAG	1 cut(s)	282
Bpu10I	CCTNAGC	1 cut(s)	816
Bpu14I	TTCGAA	1 cut(s)	754
BpuEI	CTTGAG	4 cut(s)	152, 701, 1092, 1469
BpuMI	CCSGG	1 cut(s)	126
BsaHI	GRCGYC	2 cut(s)	243, 409
BsaJI	CCNNGG	3 cut(s)	105, 189, 204
BsaXI	ACNNNNNCTCC	2 cut(s)	427, 457
Bsc4I	CCNNNNNNNGG	2 cut(s)	767, 797
Bse1I	ACTGG	4 cut(s)	299, 321, 519, 751
Bse3DI	GCAATG	4 cut(s)	543, 1317, 1407, 1460
BseBI	CCWGG	2 cut(s)	414, 792
BseDI	CCNNGG	3 cut(s)	105, 189, 204
BseGI	GGATG	2 cut(s)	514, 967
BseLI	CCNNNNNNNGG	2 cut(s)	767, 797
BseMI	GCAATG	4 cut(s)	543, 1317, 1407, 1460
BseNI	ACTGG	4 cut(s)	299, 321, 519, 751
BseRI	GAGGAG	6 cut(s)	46, 49, 52, 55, 58, 556
BseSI	GKGCMC	1 cut(s)	126
BseXI	GCAGC	3 cut(s)	118, 473, 1319
BseYI	CCCAGC	1 cut(s)	119
BsgI	GTGCAG	2 cut(s)	432, 612
Bsh1236I	CGCG	1 cut(s)	153
BshFI	GGCC	7 cut(s)	18, 124, 188, 750, 760, 1212, 1417
BsiHKCI	CYCGRG	1 cut(s)	765
BsiSI	CCGG	1 cut(s)	126
BslI	CCNNNNNNNGG	2 cut(s)	767, 797
BsmAI	GTCTC	2 cut(s)	1177, 1251
BsnI	GGCC	7 cut(s)	18, 124, 188, 750, 760, 1212, 1417
BsoBI	CYCGRG	1 cut(s)	765
Bsp119I	TTCGAA	1 cut(s)	754
Bsp120I	GGGCCC	1 cut(s)	122
Bsp1286I	GDGCHC	1 cut(s)	126
Bsp143I	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
Bsp19I	CCATGG	1 cut(s)	105
BspACI	CCGC	3 cut(s)	87, 102, 910
BspANI	GGCC	7 cut(s)	18, 124, 188, 750, 760, 1212, 1417
BspFNI	CGCG	1 cut(s)	153
BspLI	GGNNCC	2 cut(s)	37, 124
BspMAI	CTGCAG	1 cut(s)	463
BspMI	ACCTGC	2 cut(s)	584, 1183
BspOI	GCTAGC	1 cut(s)	291
BspQI	GCTCTTC	1 cut(s)	1437
BspT104I	TTCGAA	1 cut(s)	754
BspTI	CTTAAG	1 cut(s)	400
BsrDI	GCAATG	4 cut(s)	543, 1317, 1407, 1460
BsrI	ACTGG	4 cut(s)	299, 321, 519, 751
BssECI	CCNNGG	3 cut(s)	105, 189, 204
BssMI	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
BssNAI	GTATAC	1 cut(s)	1153
BssNI	GRCGYC	2 cut(s)	243, 409
BssT1I	CCWWGG	3 cut(s)	105, 189, 204
Bst1107I	GTATAC	1 cut(s)	1153
Bst2UI	CCWGG	2 cut(s)	414, 792
Bst4CI	ACNGT	3 cut(s)	439, 974, 1408
Bst6I	CTCTTC	4 cut(s)	189, 727, 1139, 1437
BstACI	GRCGYC	2 cut(s)	243, 409
BstAFI	CTTAAG	1 cut(s)	400
BstBI	TTCGAA	1 cut(s)	754
BstC8I	GCNNGC	7 cut(s)	289, 530, 534, 762, 813, 993, 1012
BstDEI	CTNAG	5 cut(s)	726, 816, 1130, 1193, 1376
BstDSI	CCRYGG	1 cut(s)	105
BstEII	GGTNACC	1 cut(s)	139
BstF5I	GGATG	2 cut(s)	514, 967
BstFNI	CGCG	1 cut(s)	153
BstH2I	RGCGCY	2 cut(s)	267, 321
BstHHI	GCGC	3 cut(s)	153, 266, 320
BstKTI	GATC	6 cut(s)	201, 569, 1171, 1240, 1285, 1353
BstMAI	GTCTC	2 cut(s)	1177, 1251
BstMBI	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
BstMWI	GCNNNNNNNGC	1 cut(s)	215
BstNI	CCWGG	2 cut(s)	414, 792
BstNSI	RCATGY	1 cut(s)	532
BstPI	GGTNACC	1 cut(s)	139
BstSCI	CCNGG	3 cut(s)	124, 412, 790
BstSFI	CTRYAG	1 cut(s)	459
BstSLI	GKGCMC	1 cut(s)	126
BstUI	CGCG	1 cut(s)	153
BstV1I	GCAGC	3 cut(s)	118, 473, 1319
BstV2I	GAAGAC	2 cut(s)	34, 246
BstX2I	RGATCY	2 cut(s)	1168, 1237
BstYI	RGATCY	2 cut(s)	1168, 1237
BstZ17I	GTATAC	1 cut(s)	1153
BsuRI	GGCC	7 cut(s)	18, 124, 188, 750, 760, 1212, 1417
BtgI	CCRYGG	1 cut(s)	105
BtrI	CACGTC	1 cut(s)	423
BtsCI	GGATG	2 cut(s)	514, 967
BtsIMutI	CAGTG	5 cut(s)	306, 328, 744, 869, 1260
BveI	ACCTGC	2 cut(s)	584, 1183
Cac8I	GCNNGC	7 cut(s)	289, 530, 534, 762, 813, 993, 1012
CfoI	GCGC	3 cut(s)	153, 266, 320
Cfr13I	GGNCC	3 cut(s)	122, 123, 785
CseI	GACGC	2 cut(s)	251, 1130
CsiI	ACCWGGT	1 cut(s)	790
Csp6I	GTAC	3 cut(s)	160, 175, 522
CspCI	CAANNNNNGTGG	2 cut(s)	867, 902
CviAII	CATG	7 cut(s)	106, 494, 529, 808, 922, 958, 979
CviQI	GTAC	3 cut(s)	160, 175, 522
DdeI	CTNAG	5 cut(s)	726, 816, 1130, 1193, 1376
DpnI	GATC	6 cut(s)	200, 568, 1170, 1239, 1284, 1352
DpnII	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
EaeI	YGGCCR	1 cut(s)	186
Eam1104I	CTCTTC	4 cut(s)	189, 727, 1139, 1437
EarI	CTCTTC	4 cut(s)	189, 727, 1139, 1437
EciI	GGCGGA	1 cut(s)	91
Eco130I	CCWWGG	3 cut(s)	105, 189, 204
Eco147I	AGGCCT	1 cut(s)	18
Eco24I	GRGCYC	1 cut(s)	126
Eco32I	GATATC	1 cut(s)	360
Eco47I	GGWCC	1 cut(s)	785
Eco57I	CTGAAG	3 cut(s)	240, 813, 1284
Eco88I	CYCGRG	1 cut(s)	765
Eco91I	GGTNACC	1 cut(s)	139
EcoO65I	GGTNACC	1 cut(s)	139
EcoRI	GAATTC	2 cut(s)	273, 481
EcoRII	CCWGG	2 cut(s)	412, 790
EcoRV	GATATC	1 cut(s)	360
EcoT14I	CCWWGG	3 cut(s)	105, 189, 204
EcoT38I	GRGCYC	1 cut(s)	126
ErhI	CCWWGG	3 cut(s)	105, 189, 204
FaeI	CATG	7 cut(s)	109, 497, 532, 811, 925, 961, 982
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FatI	CATG	7 cut(s)	105, 493, 528, 807, 921, 957, 978
FblI	GTMKAC	2 cut(s)	406, 1152
Fnu4HI	GCNGC	3 cut(s)	132, 462, 1308
FokI	GGATG	2 cut(s)	501, 974
FriOI	GRGCYC	1 cut(s)	126
Fsp4HI	GCNGC	3 cut(s)	132, 462, 1308
FspBI	CTAG	4 cut(s)	278, 288, 1115, 1179
GlaI	GCGC	3 cut(s)	152, 265, 319
GluI	GCNGC	3 cut(s)	132, 462, 1308
GsaI	CCCAGC	1 cut(s)	123
GsuI	CTGGAG	1 cut(s)	282
HaeII	RGCGCY	2 cut(s)	267, 321
HaeIII	GGCC	7 cut(s)	18, 124, 188, 750, 760, 1212, 1417
HapII	CCGG	1 cut(s)	126
HgaI	GACGC	2 cut(s)	251, 1130
HhaI	GCGC	3 cut(s)	153, 266, 320
Hin1I	GRCGYC	2 cut(s)	243, 409
Hin1II	CATG	7 cut(s)	109, 497, 532, 811, 925, 961, 982
Hin6I	GCGC	3 cut(s)	151, 264, 318
HinP1I	GCGC	3 cut(s)	151, 264, 318
HincII	GTYRAC	1 cut(s)	984
HindII	GTYRAC	1 cut(s)	984
HindIII	AAGCTT	1 cut(s)	902
HinfI	GANTC	6 cut(s)	26, 50, 508, 544, 584, 833
HpaII	CCGG	1 cut(s)	126
HphI	GGTGA	5 cut(s)	133, 349, 700, 1153, 1346
Hpy166II	GTNNAC	5 cut(s)	407, 744, 788, 984, 1153
Hpy188I	TCNGA	7 cut(s)	49, 385, 549, 832, 838, 1030, 1395
Hpy188III	TCNNGA	7 cut(s)	486, 680, 936, 1109, 1241, 1275, 1348
Hpy8I	GTNNAC	5 cut(s)	407, 744, 788, 984, 1153
HpyAV	CCTTC	9 cut(s)	315, 328, 660, 679, 750, 761, 1011, 1259, 1390
HpyCH4III	ACNGT	3 cut(s)	439, 974, 1408
HpyCH4IV	ACGT	3 cut(s)	409, 422, 476
HpyCH4V	TGCA	9 cut(s)	251, 449, 461, 514, 580, 593, 863, 1010, 1253
HpyF10VI	GCNNNNNNNGC	1 cut(s)	215
HpyF3I	CTNAG	5 cut(s)	726, 816, 1130, 1193, 1376
HpySE526I	ACGT	3 cut(s)	409, 422, 476
Hsp92I	GRCGYC	2 cut(s)	243, 409
Hsp92II	CATG	7 cut(s)	109, 497, 532, 811, 925, 961, 982
HspAI	GCGC	3 cut(s)	151, 264, 318
Kzo9I	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
LguI	GCTCTTC	1 cut(s)	1437
LmnI	GCTCC	4 cut(s)	41, 128, 296, 448
Lsp1109I	GCAGC	3 cut(s)	118, 473, 1319
LweI	GCATC	4 cut(s)	523, 872, 997, 1305
MabI	ACCWGGT	1 cut(s)	790
MaeI	CTAG	4 cut(s)	278, 288, 1115, 1179
MaeII	ACGT	3 cut(s)	409, 422, 476
MaeIII	GTNAC	4 cut(s)	139, 390, 1054, 1196
MalI	GATC	6 cut(s)	200, 568, 1170, 1239, 1284, 1352
MboI	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
MflI	RGATCY	2 cut(s)	1168, 1237
MhlI	GDGCHC	1 cut(s)	126
MlsI	TGGCCA	1 cut(s)	188
MluNI	TGGCCA	1 cut(s)	188
MlyI	GAGTC	3 cut(s)	20, 502, 553
Mox20I	TGGCCA	1 cut(s)	188
MroXI	GAANNNNTTC	3 cut(s)	649, 1341, 1389
MscI	TGGCCA	1 cut(s)	188
MseI	TTAA	4 cut(s)	401, 657, 852, 1424
MslI	CAYNNNNRTG	2 cut(s)	299, 880
Msp20I	TGGCCA	1 cut(s)	188
MspCI	CTTAAG	1 cut(s)	400
MspI	CCGG	1 cut(s)	126
MspR9I	CCNGG	3 cut(s)	126, 414, 792
MvaI	CCWGG	2 cut(s)	414, 792
MvnI	CGCG	1 cut(s)	153
MwoI	GCNNNNNNNGC	1 cut(s)	215
NciI	CCSGG	1 cut(s)	126
NcoI	CCATGG	1 cut(s)	105
NdeII	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
NheI	GCTAGC	1 cut(s)	287
NlaIII	CATG	7 cut(s)	109, 497, 532, 811, 925, 961, 982
NlaIV	GGNNCC	2 cut(s)	37, 124
NmuCI	GTSAC	3 cut(s)	139, 1054, 1196
NspI	RCATGY	1 cut(s)	532
NspV	TTCGAA	1 cut(s)	754
OliI	CACNNNNGTG	1 cut(s)	299
PaeI	GCATGC	1 cut(s)	532
PaeR7I	CTCGAG	1 cut(s)	765
PceI	AGGCCT	1 cut(s)	18
PciSI	GCTCTTC	1 cut(s)	1437
PdmI	GAANNNNTTC	3 cut(s)	649, 1341, 1389
PfeI	GAWTC	3 cut(s)	50, 584, 833
PkrI	GCNGC	3 cut(s)	133, 463, 1309
PleI	GAGTC	3 cut(s)	20, 502, 552
PpsI	GAGTC	3 cut(s)	20, 502, 552
Psp1406I	AACGTT	1 cut(s)	476
Psp6I	CCWGG	2 cut(s)	412, 790
PspEI	GGTNACC	1 cut(s)	139
PspFI	CCCAGC	1 cut(s)	119
PspGI	CCWGG	2 cut(s)	412, 790
PspN4I	GGNNCC	2 cut(s)	37, 124
PspOMI	GGGCCC	1 cut(s)	122
PspPI	GGNCC	3 cut(s)	122, 123, 785
PspXI	VCTCGAGB	1 cut(s)	765
PstI	CTGCAG	1 cut(s)	463
PsuI	RGATCY	2 cut(s)	1168, 1237
RsaI	GTAC	3 cut(s)	161, 176, 523
RsaNI	GTAC	3 cut(s)	160, 175, 522
RseI	CAYNNNNRTG	2 cut(s)	299, 880
SapI	GCTCTTC	1 cut(s)	1437
SaqAI	TTAA	4 cut(s)	401, 657, 852, 1424
SatI	GCNGC	3 cut(s)	132, 462, 1308
Sau3AI	GATC	6 cut(s)	198, 566, 1168, 1237, 1282, 1350
Sau96I	GGNCC	3 cut(s)	122, 123, 785
SchI	GAGTC	3 cut(s)	20, 502, 553
ScrFI	CCNGG	3 cut(s)	126, 414, 792
SduI	GDGCHC	1 cut(s)	126
SexAI	ACCWGGT	1 cut(s)	790
SfaNI	GCATC	4 cut(s)	523, 872, 997, 1305
SfcI	CTRYAG	1 cut(s)	459
Sfr274I	CTCGAG	1 cut(s)	765
SfuI	TTCGAA	1 cut(s)	754
SinI	GGWCC	1 cut(s)	785
SlaI	CTCGAG	1 cut(s)	765
SmiMI	CAYNNNNRTG	2 cut(s)	299, 880
SmlI	CTYRAG	6 cut(s)	167, 400, 680, 765, 1107, 1448
SmoI	CTYRAG	6 cut(s)	167, 400, 680, 765, 1107, 1448
SphI	GCATGC	1 cut(s)	532
SseBI	AGGCCT	1 cut(s)	18
SsiI	CCGC	3 cut(s)	87, 102, 910
SspI	AATATT	1 cut(s)	559
SspMI	CTAG	4 cut(s)	278, 288, 1115, 1179
StuI	AGGCCT	1 cut(s)	18
StyD4I	CCNGG	3 cut(s)	124, 412, 790
StyI	CCWWGG	3 cut(s)	105, 189, 204
TaaI	ACNGT	3 cut(s)	439, 974, 1408
TaiI	ACGT	3 cut(s)	412, 425, 479
TaqI	TCGA	3 cut(s)	24, 754, 766
TaqII	GACCGA	1 cut(s)	127
TatI	WGTACW	1 cut(s)	521
TfiI	GAWTC	3 cut(s)	50, 584, 833
Tru1I	TTAA	4 cut(s)	401, 657, 852, 1424
Tru9I	TTAA	4 cut(s)	401, 657, 852, 1424
TscAI	CASTG	5 cut(s)	306, 328, 751, 876, 1267
TseFI	GTSAC	3 cut(s)	139, 1054, 1196
TseI	GCWGC	3 cut(s)	131, 461, 1307
Tsp45I	GTSAC	3 cut(s)	139, 1054, 1196
TspDTI	ATGAA	3 cut(s)	618, 910, 946
TspGWI	ACGGA	1 cut(s)	28
TspRI	CASTG	5 cut(s)	306, 328, 751, 876, 1267
Vha464I	CTTAAG	1 cut(s)	400
VpaK11BI	GGWCC	1 cut(s)	785
XapI	RAATTY	3 cut(s)	273, 481, 1466
XceI	RCATGY	1 cut(s)	532
XcmI	CCANNNNNNNNNTGG	1 cut(s)	151
XhoI	CTCGAG	1 cut(s)	765
XmiI	GTMKAC	2 cut(s)	406, 1152
XmnI	GAANNNNTTC	3 cut(s)	649, 1341, 1389
XspI	CTAG	4 cut(s)	278, 288, 1115, 1179
ZraI	GACGTC	1 cut(s)	410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.