Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0006183

Physical Location & Seq

Genomic Coordinates Reverse (-)

43240600 .. 43247140

Gene Structure 6541 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G019950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGGAATGGAATGGACTTGTTAATAATTTTGTTTGTGTTGGCCTCTATATGAGTTTCCTCAAAGGCCTCATCGACTCTTTAGGCTCCGTCTTCTCCGAATCCTCCTCCTCCTCCTCATACCCAAACTCTACCGCTTCCAATTCCTCCGCCATGGACGGCGTGGCTGGGCCCGGAGCTGCTTCCGTCACGAACGAGCGCGTGGCGTACAAGCTCAAGGGGTACTTTGATCTGGCCAAGGAAGAGATCGCCAAGGCTGTTAGGGCTGAAGAATGGGGCTTGGTTGAAGACGCCGTTGCACACTATAATAGCGCCCAGAGAATTCTAGCCGAAGCTAGCTCCACTCCAGTGCCTTCCTTTATCAGCGCCAGTGAGCGGGAGAAGGTGAAATCGTATCGTCAAAAGATATCAAAATGGCAGGGTCAAGTTTCTGATAGGTTACAAGTCTTAAGTAGACGTCCAGGTGGTACGTCCATAAGCAAGACTGTAGCTCCTGCACGAACTACTGCAGCTACATCAACAACGTTGAATTCACGAAATCATGTGATACCCAAGACTCTGCATCCCAGTACAAGCATGCCAGCAACGAGGAGTCAGACCAATAATATTGTAAGATCAAAACCTGTGCAAGATTCTGGTGCAGGTTATGATGAAAAGTTGGTTGAAATGATAAATAGTGCCATAGTGGATAGAAGTCCTTCTGTTAAATGGGAAGATGTTGGTGAGAAGATTATGGCATACCTTCATTTCTTATGGTTATCTTTACCGACTAAGAGAAGAGATTTGTTCACTGGCCTTCGGAGGCCAGCTCGAGGTCTGCTTCTGTTTGGTCCACCTGGTAATGGGAAAACCATGCTTGCTAAGGCAGTTGCTTCAGAATCAGAGGCAACCTTTTTTAATGTATCTGCATCTTCACTGACATCAAAATGGGTGGGAGAGGCTGAAAAGCTCGTGCGGACACTTTTCATGGTTGCTATATCCAGACAGCCATCTGTAATTTTCATGGATGAGATTGATAGTATCATGTCAACAAGGCAGGCAAACGAAAATGATGCAAGCAGAAGGTTGAAGTCAGAGTTTCTAATTCAGTTTGATGGAGTGACATCTAATCCTAATGATCTAGTAATTGTGATTGGTGCTACTAATAAGCCTCAAGAACTAGATGACGCAGTTCTTAGGAGATTGGTGAAGAGAGTATACATACCTTTACCAGATCTACCTGCTAGGAGACTTCTCCTAAGTCACAAACTCAAAGGCCAAGCATTTTCCTTACCAAGTGGAGATCTTGAACGACTTGCAAGAGACACTGAAGGTTATTCAGGAAGTGATCTACAAGCATTGTGTGAAGAAGCAGCAATGATGCCAATTAGGGAACTTGGTGAAAACATTCTCACCATCAAGGCAAATCAGGTAAGACCACTAAGAATTGAAGATTTTCAGAAGGCAATGACTGTAATCAGGCCAAGTTTAAGCAAAAGCAAGTGGGAAGAGCTTGAACAATGGAACGAGGAATTTGGGTCTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0001578 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0006810 GO:0006928 GO:0006996 GO:0006997 GO:0006998 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008017 GO:0008088 GO:0008089 GO:0008092 GO:0008150 GO:0008568 GO:0009987 GO:0010256 GO:0010458 GO:0010970 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019896 GO:0022402 GO:0022411 GO:0022607 GO:0030496 GO:0030705 GO:0031090 GO:0031468 GO:0031965 GO:0031967 GO:0031975 GO:0034214 GO:0034643 GO:0042623 GO:0043014 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0046907 GO:0047497 GO:0048285 GO:0048487 GO:0051013 GO:0051179 GO:0051228 GO:0051230 GO:0051234 GO:0051259 GO:0051260 GO:0051301 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0061024 GO:0061640 GO:0065003 GO:0071840 GO:0072384 GO:0090148 GO:0098930 GO:0099111 GO:0140014 GO:0140096 GO:1902850 GO:1903008 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Vps4_C

Protein Analysis

507

Amino Acids

55.88

Weight (kDa)

9.05

Isoelectric Point (pI)

50.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	272 - 402	4.3e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	427 - 461	9.1e-14	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45500 AT2G45500 AT2G45500 AT2G45500 AT2G45500
fragaria_vesca	FvH4_7g12180 FvH4_7g12180
malus_domestica	MD02G1200800.v1.1
prunus_persica	Prupe.2G148300_v2.0.a1 Prupe.2G148300_v2.0.a1 Prupe.2G148300_v2.0.a1
pyrus_communis	pycom02g16400
rosa_chinensis	RchiOBHm_Chr1g0350931
rosa_laevigata	RLG00000028440
rosa_multiflora	Rmu_sc0002665.1_g000019 Rmu_sc0006458.1_g000018
rosa_roxburghii	Rroxscaffold_4G00304290
rosa_rugosa	Rorug01G0075600 Rorug01G0215400 Rorug01G0215400 Rorug06G0023300
rosa_samantha	Rh1AG230100 Rh1BG075200 Rh1BG200000 Rh1CG214500 Rh1DG227800
rosa_wichuraiana	Rw1G019950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	457
Acc36I	ACCTGC	2 cut(s)	629, 1225
AccBSI	CCGCTC	1 cut(s)	373
AccI	GTMKAC	2 cut(s)	451, 1194
AccII	CGCG	1 cut(s)	198
AciI	CCGC	4 cut(s)	132, 147, 373, 952
AclI	AACGTT	1 cut(s)	521
AcoI	YGGCCR	1 cut(s)	231
AcsI	RAATTY	3 cut(s)	318, 526, 1508
AcuI	CTGAAG	3 cut(s)	285, 855, 1326
AcyI	GRCGYC	2 cut(s)	288, 454
AfaI	GTAC	4 cut(s)	206, 221, 466, 568
AfiI	CCNNNNNNNGG	3 cut(s)	372, 809, 839
AflII	CTTAAG	1 cut(s)	445
AgsI	TTSAA	7 cut(s)	284, 526, 662, 1066, 1286, 1427, 1493
AjnI	CCWGG	2 cut(s)	457, 832
AleI	CACNNNNGTG	1 cut(s)	344
AluBI	AGCT	9 cut(s)	176, 211, 332, 336, 488, 509, 806, 946, 1489
AluI	AGCT	9 cut(s)	176, 211, 332, 336, 488, 509, 806, 946, 1489
Alw26I	GTCTC	2 cut(s)	1219, 1293
Ama87I	CYCGRG	1 cut(s)	807
AoxI	GGCC	8 cut(s)	40, 64, 167, 231, 790, 800, 1252, 1457
ApaI	GGGCCC	1 cut(s)	171
ApeKI	GCWGC	3 cut(s)	176, 506, 1349
ApoI	RAATTY	3 cut(s)	318, 526, 1508
Asp700I	GAANNNNTTC	3 cut(s)	694, 1383, 1431
AspLEI	GCGC	3 cut(s)	198, 311, 365
AspS9I	GGNCC	3 cut(s)	167, 168, 827
AsuC2I	CCSGG	1 cut(s)	171
AsuHPI	GGTGA	5 cut(s)	394, 731, 1195, 1381, 1388
AsuNHI	GCTAGC	1 cut(s)	332
AvaI	CYCGRG	1 cut(s)	807
AvaII	GGWCC	1 cut(s)	827
BaeGI	GKGCMC	1 cut(s)	171
BalI	TGGCCA	1 cut(s)	233
BanII	GRGCYC	1 cut(s)	171
BarI	GAAGNNNNNNTAC	2 cut(s)	1178, 1210
BauI	CACGAG	1 cut(s)	947
BbsI	GAAGAC	2 cut(s)	82, 291
BbvI	GCAGC	3 cut(s)	163, 518, 1361
BccI	CCATC	3 cut(s)	994, 1085, 1400
BceAI	ACGGC	2 cut(s)	172, 275
BcgI	CGANNNNNNTGC	2 cut(s)	1031, 1065
BciT130I	CCWGG	2 cut(s)	459, 834
BcnI	CCSGG	1 cut(s)	171
BcoDI	GTCTC	2 cut(s)	1219, 1293
BfaI	CTAG	5 cut(s)	323, 333, 1118, 1157, 1221
BfmI	CTRYAG	2 cut(s)	483, 504
BfoI	RGCGCY	2 cut(s)	312, 366
BfrI	CTTAAG	1 cut(s)	445
BfuAI	ACCTGC	2 cut(s)	629, 1225
BglII	AGATCT	2 cut(s)	1210, 1279
BisI	GCNGC	3 cut(s)	177, 507, 1350
BlsI	GCNGC	3 cut(s)	178, 508, 1351
Bme1390I	CCNGG	3 cut(s)	171, 459, 834
Bme18I	GGWCC	1 cut(s)	827
BmeT110I	CYCGRG	1 cut(s)	807
BmgT120I	GGNCC	3 cut(s)	167, 168, 827
BmiI	GGNNCC	2 cut(s)	85, 169
BmrFI	CCNGG	3 cut(s)	171, 459, 834
BmrI	ACTGGG	1 cut(s)	558
BmsI	GCATC	4 cut(s)	568, 914, 1039, 1347
BmtI	GCTAGC	1 cut(s)	336
BmuI	ACTGGG	1 cut(s)	558
BpiI	GAAGAC	2 cut(s)	82, 291
BplI	GAGNNNNNCTC	2 cut(s)	790, 822
BpmI	CTGGAG	1 cut(s)	327
Bpu10I	CCTNAGC	1 cut(s)	858
BpuEI	CTTGAG	2 cut(s)	197, 1134
BpuMI	CCSGG	1 cut(s)	171
BsaHI	GRCGYC	2 cut(s)	288, 454
BsaJI	CCNNGG	3 cut(s)	150, 234, 249
BsaXI	ACNNNNNCTCC	2 cut(s)	472, 502
Bsc4I	CCNNNNNNNGG	3 cut(s)	372, 809, 839
Bse1I	ACTGG	4 cut(s)	344, 366, 564, 793
Bse3DI	GCAATG	2 cut(s)	1359, 1449
BseBI	CCWGG	2 cut(s)	459, 834
BseDI	CCNNGG	3 cut(s)	150, 234, 249
BseGI	GGATG	2 cut(s)	559, 1009
BseLI	CCNNNNNNNGG	3 cut(s)	372, 809, 839
BseMI	GCAATG	2 cut(s)	1359, 1449
BseNI	ACTGG	4 cut(s)	344, 366, 564, 793
BseRI	GAGGAG	5 cut(s)	94, 97, 100, 103, 601
BseSI	GKGCMC	1 cut(s)	171
BseXI	GCAGC	3 cut(s)	163, 518, 1361
BseYI	CCCAGC	1 cut(s)	164
BsgI	GTGCAG	2 cut(s)	477, 657
Bsh1236I	CGCG	1 cut(s)	198
BshFI	GGCC	8 cut(s)	42, 66, 169, 233, 792, 802, 1254, 1459
BsiHKCI	CYCGRG	1 cut(s)	807
BsiSI	CCGG	1 cut(s)	171
BslI	CCNNNNNNNGG	3 cut(s)	372, 809, 839
BsmAI	GTCTC	2 cut(s)	1219, 1293
BsnI	GGCC	8 cut(s)	42, 66, 169, 233, 792, 802, 1254, 1459
BsoBI	CYCGRG	1 cut(s)	807
Bsp120I	GGGCCC	1 cut(s)	167
Bsp1286I	GDGCHC	1 cut(s)	171
Bsp143I	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
Bsp19I	CCATGG	1 cut(s)	150
BspACI	CCGC	4 cut(s)	132, 147, 373, 952
BspANI	GGCC	8 cut(s)	42, 66, 169, 233, 792, 802, 1254, 1459
BspFNI	CGCG	1 cut(s)	198
BspLI	GGNNCC	2 cut(s)	85, 169
BspMAI	CTGCAG	1 cut(s)	508
BspMI	ACCTGC	2 cut(s)	629, 1225
BspOI	GCTAGC	1 cut(s)	336
BspQI	GCTCTTC	1 cut(s)	1479
BspTI	CTTAAG	1 cut(s)	445
BsrBI	CCGCTC	1 cut(s)	373
BsrDI	GCAATG	2 cut(s)	1359, 1449
BsrI	ACTGG	4 cut(s)	344, 366, 564, 793
BssECI	CCNNGG	3 cut(s)	150, 234, 249
BssMI	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
BssNAI	GTATAC	1 cut(s)	1195
BssNI	GRCGYC	2 cut(s)	288, 454
BssSI	CACGAG	1 cut(s)	947
BssT1I	CCWWGG	3 cut(s)	150, 234, 249
Bst1107I	GTATAC	1 cut(s)	1195
Bst2BI	CACGAG	1 cut(s)	947
Bst2UI	CCWGG	2 cut(s)	459, 834
Bst4CI	ACNGT	2 cut(s)	484, 1450
Bst6I	CTCTTC	4 cut(s)	234, 769, 1181, 1479
BstACI	GRCGYC	2 cut(s)	288, 454
BstAFI	CTTAAG	1 cut(s)	445
BstC8I	GCNNGC	7 cut(s)	334, 575, 579, 804, 855, 1035, 1054
BstDEI	CTNAG	5 cut(s)	768, 858, 1172, 1235, 1418
BstDSI	CCRYGG	1 cut(s)	150
BstF5I	GGATG	2 cut(s)	559, 1009
BstFNI	CGCG	1 cut(s)	198
BstH2I	RGCGCY	2 cut(s)	312, 366
BstHHI	GCGC	3 cut(s)	198, 311, 365
BstKTI	GATC	7 cut(s)	229, 246, 614, 1117, 1213, 1282, 1327
BstMAI	GTCTC	2 cut(s)	1219, 1293
BstMBI	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
BstMWI	GCNNNNNNNGC	1 cut(s)	260
BstNI	CCWGG	2 cut(s)	459, 834
BstNSI	RCATGY	1 cut(s)	577
BstSCI	CCNGG	3 cut(s)	169, 457, 832
BstSFI	CTRYAG	2 cut(s)	483, 504
BstSLI	GKGCMC	1 cut(s)	171
BstUI	CGCG	1 cut(s)	198
BstV1I	GCAGC	3 cut(s)	163, 518, 1361
BstV2I	GAAGAC	2 cut(s)	82, 291
BstX2I	RGATCY	2 cut(s)	1210, 1279
BstYI	RGATCY	2 cut(s)	1210, 1279
BstZ17I	GTATAC	1 cut(s)	1195
BsuRI	GGCC	8 cut(s)	42, 66, 169, 233, 792, 802, 1254, 1459
BtgI	CCRYGG	1 cut(s)	150
BtsCI	GGATG	2 cut(s)	559, 1009
BtsIMutI	CAGTG	5 cut(s)	351, 373, 786, 911, 1302
BveI	ACCTGC	2 cut(s)	629, 1225
Cac8I	GCNNGC	7 cut(s)	334, 575, 579, 804, 855, 1035, 1054
CfoI	GCGC	3 cut(s)	198, 311, 365
Cfr13I	GGNCC	3 cut(s)	167, 168, 827
CseI	GACGC	2 cut(s)	296, 1172
CsiI	ACCWGGT	1 cut(s)	832
Csp6I	GTAC	4 cut(s)	205, 220, 465, 567
CspCI	CAANNNNNGTGG	2 cut(s)	909, 944
CviAII	CATG	7 cut(s)	151, 539, 574, 850, 964, 1000, 1021
CviQI	GTAC	4 cut(s)	205, 220, 465, 567
DdeI	CTNAG	5 cut(s)	768, 858, 1172, 1235, 1418
DpnI	GATC	7 cut(s)	228, 245, 613, 1116, 1212, 1281, 1326
DpnII	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
EaeI	YGGCCR	1 cut(s)	231
Eam1104I	CTCTTC	4 cut(s)	234, 769, 1181, 1479
EarI	CTCTTC	4 cut(s)	234, 769, 1181, 1479
EciI	GGCGGA	1 cut(s)	136
Eco130I	CCWWGG	3 cut(s)	150, 234, 249
Eco147I	AGGCCT	1 cut(s)	66
Eco24I	GRGCYC	1 cut(s)	171
Eco32I	GATATC	1 cut(s)	405
Eco47I	GGWCC	1 cut(s)	827
Eco57I	CTGAAG	3 cut(s)	285, 855, 1326
Eco88I	CYCGRG	1 cut(s)	807
EcoRI	GAATTC	2 cut(s)	318, 526
EcoRII	CCWGG	2 cut(s)	457, 832
EcoRV	GATATC	1 cut(s)	405
EcoT14I	CCWWGG	3 cut(s)	150, 234, 249
EcoT38I	GRGCYC	1 cut(s)	171
ErhI	CCWWGG	3 cut(s)	150, 234, 249
FaeI	CATG	7 cut(s)	154, 542, 577, 853, 967, 1003, 1024
FalI	AAGNNNNNCTT	2 cut(s)	206, 238
FatI	CATG	7 cut(s)	150, 538, 573, 849, 963, 999, 1020
FauI	CCCGC	1 cut(s)	366
FblI	GTMKAC	2 cut(s)	451, 1194
Fnu4HI	GCNGC	3 cut(s)	177, 507, 1350
FokI	GGATG	2 cut(s)	546, 1016
FriOI	GRGCYC	1 cut(s)	171
Fsp4HI	GCNGC	3 cut(s)	177, 507, 1350
FspBI	CTAG	5 cut(s)	323, 333, 1118, 1157, 1221
GlaI	GCGC	3 cut(s)	197, 310, 364
GluI	GCNGC	3 cut(s)	177, 507, 1350
GsaI	CCCAGC	1 cut(s)	168
GsuI	CTGGAG	1 cut(s)	327
HaeII	RGCGCY	2 cut(s)	312, 366
HaeIII	GGCC	8 cut(s)	42, 66, 169, 233, 792, 802, 1254, 1459
HapII	CCGG	1 cut(s)	171
HgaI	GACGC	2 cut(s)	296, 1172
HhaI	GCGC	3 cut(s)	198, 311, 365
Hin1I	GRCGYC	2 cut(s)	288, 454
Hin1II	CATG	7 cut(s)	154, 542, 577, 853, 967, 1003, 1024
Hin6I	GCGC	3 cut(s)	196, 309, 363
HinP1I	GCGC	3 cut(s)	196, 309, 363
HincII	GTYRAC	1 cut(s)	1026
HindII	GTYRAC	1 cut(s)	1026
HinfI	GANTC	6 cut(s)	74, 98, 553, 589, 629, 875
HpaII	CCGG	1 cut(s)	171
HphI	GGTGA	5 cut(s)	394, 731, 1195, 1381, 1388
Hpy166II	GTNNAC	5 cut(s)	452, 786, 830, 1026, 1195
Hpy188I	TCNGA	8 cut(s)	97, 430, 594, 798, 874, 880, 1072, 1437
Hpy188III	TCNNGA	6 cut(s)	187, 531, 978, 1151, 1283, 1317
Hpy8I	GTNNAC	5 cut(s)	452, 786, 830, 1026, 1195
HpyAV	CCTTC	8 cut(s)	360, 373, 705, 749, 803, 1053, 1301, 1432
HpyCH4III	ACNGT	2 cut(s)	484, 1450
HpyCH4IV	ACGT	3 cut(s)	454, 467, 521
HpyCH4V	TGCA	9 cut(s)	296, 494, 506, 559, 625, 638, 905, 1052, 1295
HpyF10VI	GCNNNNNNNGC	1 cut(s)	260
HpyF3I	CTNAG	5 cut(s)	768, 858, 1172, 1235, 1418
HpySE526I	ACGT	3 cut(s)	454, 467, 521
Hsp92I	GRCGYC	2 cut(s)	288, 454
Hsp92II	CATG	7 cut(s)	154, 542, 577, 853, 967, 1003, 1024
HspAI	GCGC	3 cut(s)	196, 309, 363
Kzo9I	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
LguI	GCTCTTC	1 cut(s)	1479
LmnI	GCTCC	4 cut(s)	89, 173, 341, 493
Lsp1109I	GCAGC	3 cut(s)	163, 518, 1361
LweI	GCATC	4 cut(s)	568, 914, 1039, 1347
MabI	ACCWGGT	1 cut(s)	832
MaeI	CTAG	5 cut(s)	323, 333, 1118, 1157, 1221
MaeII	ACGT	3 cut(s)	454, 467, 521
MaeIII	GTNAC	4 cut(s)	184, 435, 1096, 1238
MalI	GATC	7 cut(s)	228, 245, 613, 1116, 1212, 1281, 1326
MbiI	CCGCTC	1 cut(s)	373
MboI	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
MflI	RGATCY	2 cut(s)	1210, 1279
MhlI	GDGCHC	1 cut(s)	171
MlsI	TGGCCA	1 cut(s)	233
MluNI	TGGCCA	1 cut(s)	233
MlyI	GAGTC	3 cut(s)	68, 547, 598
Mox20I	TGGCCA	1 cut(s)	233
MroXI	GAANNNNTTC	3 cut(s)	694, 1383, 1431
MscI	TGGCCA	1 cut(s)	233
MseI	TTAA	5 cut(s)	21, 446, 702, 894, 1466
MslI	CAYNNNNRTG	2 cut(s)	344, 922
Msp20I	TGGCCA	1 cut(s)	233
MspCI	CTTAAG	1 cut(s)	445
MspI	CCGG	1 cut(s)	171
MspR9I	CCNGG	3 cut(s)	171, 459, 834
MvaI	CCWGG	2 cut(s)	459, 834
MvnI	CGCG	1 cut(s)	198
MwoI	GCNNNNNNNGC	1 cut(s)	260
NciI	CCSGG	1 cut(s)	171
NcoI	CCATGG	1 cut(s)	150
NdeII	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
NheI	GCTAGC	1 cut(s)	332
NlaIII	CATG	7 cut(s)	154, 542, 577, 853, 967, 1003, 1024
NlaIV	GGNNCC	2 cut(s)	85, 169
NmuCI	GTSAC	3 cut(s)	184, 1096, 1238
NspI	RCATGY	1 cut(s)	577
OliI	CACNNNNGTG	1 cut(s)	344
PaeI	GCATGC	1 cut(s)	577
PaeR7I	CTCGAG	1 cut(s)	807
PceI	AGGCCT	1 cut(s)	66
PciSI	GCTCTTC	1 cut(s)	1479
PdmI	GAANNNNTTC	3 cut(s)	694, 1383, 1431
PfeI	GAWTC	3 cut(s)	98, 629, 875
PkrI	GCNGC	3 cut(s)	178, 508, 1351
PleI	GAGTC	3 cut(s)	68, 547, 597
PpsI	GAGTC	3 cut(s)	68, 547, 597
Psp1406I	AACGTT	1 cut(s)	521
Psp6I	CCWGG	2 cut(s)	457, 832
PspFI	CCCAGC	1 cut(s)	164
PspGI	CCWGG	2 cut(s)	457, 832
PspN4I	GGNNCC	2 cut(s)	85, 169
PspOMI	GGGCCC	1 cut(s)	167
PspPI	GGNCC	3 cut(s)	167, 168, 827
PspXI	VCTCGAGB	1 cut(s)	807
PstI	CTGCAG	1 cut(s)	508
PsuI	RGATCY	2 cut(s)	1210, 1279
RsaI	GTAC	4 cut(s)	206, 221, 466, 568
RsaNI	GTAC	4 cut(s)	205, 220, 465, 567
RseI	CAYNNNNRTG	2 cut(s)	344, 922
SapI	GCTCTTC	1 cut(s)	1479
SaqAI	TTAA	5 cut(s)	21, 446, 702, 894, 1466
SatI	GCNGC	3 cut(s)	177, 507, 1350
Sau3AI	GATC	7 cut(s)	226, 243, 611, 1114, 1210, 1279, 1324
Sau96I	GGNCC	3 cut(s)	167, 168, 827
SchI	GAGTC	3 cut(s)	68, 547, 598
ScrFI	CCNGG	3 cut(s)	171, 459, 834
SduI	GDGCHC	1 cut(s)	171
SexAI	ACCWGGT	1 cut(s)	832
SfaNI	GCATC	4 cut(s)	568, 914, 1039, 1347
SfcI	CTRYAG	2 cut(s)	483, 504
Sfr274I	CTCGAG	1 cut(s)	807
SinI	GGWCC	1 cut(s)	827
SlaI	CTCGAG	1 cut(s)	807
SmiMI	CAYNNNNRTG	2 cut(s)	344, 922
SmlI	CTYRAG	4 cut(s)	212, 445, 807, 1149
SmoI	CTYRAG	4 cut(s)	212, 445, 807, 1149
SphI	GCATGC	1 cut(s)	577
SseBI	AGGCCT	1 cut(s)	66
SsiI	CCGC	4 cut(s)	132, 147, 373, 952
SspI	AATATT	1 cut(s)	604
SspMI	CTAG	5 cut(s)	323, 333, 1118, 1157, 1221
StuI	AGGCCT	1 cut(s)	66
StyD4I	CCNGG	3 cut(s)	169, 457, 832
StyI	CCWWGG	3 cut(s)	150, 234, 249
TaaI	ACNGT	2 cut(s)	484, 1450
TaiI	ACGT	3 cut(s)	457, 470, 524
TaqI	TCGA	2 cut(s)	72, 808
TatI	WGTACW	1 cut(s)	566
TfiI	GAWTC	3 cut(s)	98, 629, 875
Tru1I	TTAA	5 cut(s)	21, 446, 702, 894, 1466
Tru9I	TTAA	5 cut(s)	21, 446, 702, 894, 1466
TscAI	CASTG	5 cut(s)	351, 373, 793, 918, 1309
TseFI	GTSAC	3 cut(s)	184, 1096, 1238
TseI	GCWGC	3 cut(s)	176, 506, 1349
Tsp45I	GTSAC	3 cut(s)	184, 1096, 1238
TspDTI	ATGAA	4 cut(s)	663, 731, 952, 988
TspGWI	ACGGA	2 cut(s)	76, 172
TspRI	CASTG	5 cut(s)	351, 373, 793, 918, 1309
Vha464I	CTTAAG	1 cut(s)	445
VpaK11BI	GGWCC	1 cut(s)	827
XapI	RAATTY	3 cut(s)	318, 526, 1508
XceI	RCATGY	1 cut(s)	577
XcmI	CCANNNNNNNNNTGG	1 cut(s)	157
XhoI	CTCGAG	1 cut(s)	807
XmiI	GTMKAC	2 cut(s)	451, 1194
XmnI	GAANNNNTTC	3 cut(s)	694, 1383, 1431
XspI	CTAG	5 cut(s)	323, 333, 1118, 1157, 1221
ZraI	GACGTC	1 cut(s)	455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.