Rroxscaffold_4G00306910

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008806
Physical Location & Seq
Reverse (-)
27898711 .. 27901450
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306910.1

Sequence Viewer

Length: 966 bp
ATGATCCCTCTGCGCAAACTGATGAAACTGGACAAGGGCGACACAGATCGCGGCCTTCAACAACCTTCTCCCTCCACAGCCTCACCAGCAGCAGCAGCAGTCGGCCGGCAGTGCATTCTCCCACCATTTCAGCCAGGCCCAGGCCCACCAGGTGCACTCCTCTTCGACCCCAGCGTCTTCGCCAGCGCGTCGTCCTACGCGCCGACGTCAGCGCACCACCACCACCACCACTCCGTGCTGACCCTCGACCCGTCCATCCCGGGCCACCACGGGCTCGTGTTGATCCCCAAGAGCGAGGACGTCTGTCCGCCCGCCGACTTCGCCACGAGGATCGGGCTCAACCTCGGCGGGCGGACCTACTTCTCGTCCGAGGATGACTTCATGAACCGGCTCTACCGCCGGTCCCGGCCAGCCGACCACGGCCCGATCAACTCCCCAAGATGCCAGGCCGAGGGCTGCCACGCCGATCTCTCCCAGGCCAAGCACTACCACCGCCGCCACAAGGTCTGCGAGTTCCACTCCAAAGCCTCCACCGTCATCGCCAACGGGTTGACTCAGCGATTCTGTCAGCAGTGCAGCAGATTCCATCTTCTTGCGGAGTTTGACAACGGAAAACGCAGTTGCCGGAAGAGATTGGCCGATCACAACCGTCGCCGGCGAAAAACTCAGCAACCAAATCAAGAAAACCGCAGCTCTCAACAGCGGCCGGAAAGTACCCGGAATTCGTCATCAGACAATGTTACCAAGTCTCCGGCAGAATCTGCAGGTCATTCGTCTTCGGCTGTGACTGTGGCCGTGTCGCCGCCGCGAATGGGGTTGGATTGTTTTAGGCCAAGGCAGTATCAAAACACAACTTCTTCAGGGTCTTCAAGCTCACTTTTTTCTCAAGTGGGTGAAATGACCTCTTTCATTTTTAAGGGTTCCAAGGAAGCTAGAGCTAGTAGCCAGGAAGGAAGCAAGACTTAG

Protein Analysis

321

Amino Acids

35.18

Weight (kDa)

9.78

Isoelectric Point (pI)

62.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 148 - 221 1.4e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AatII GACGTC 2 cut(s) 209, 303
Acc16I TGCGCA 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 755
AccII CGCG 4 cut(s) 51, 188, 200, 808
AclWI GGATC 2 cut(s) 277, 338
AcoI YGGCCR 5 cut(s) 103, 407, 636, 704, 792
AcsI RAATTY 1 cut(s) 721
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 2 cut(s) 206, 300
AdeI CACNNNGTG 2 cut(s) 152, 235
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 4 cut(s) 140, 451, 502, 812
AgsI TTSAA 2 cut(s) 59, 870
AjnI CCWGG 6 cut(s) 133, 139, 148, 444, 474, 945
AluBI AGCT 4 cut(s) 693, 873, 932, 938
AluI AGCT 4 cut(s) 693, 873, 932, 938
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 1 cut(s) 753
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 2 cut(s) 277, 338
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 259
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 6 cut(s) 89, 92, 95, 456, 576, 690
ApoI RAATTY 1 cut(s) 721
AspLEI GCGC 4 cut(s) 15, 188, 202, 214
AspS9I GGNCC 6 cut(s) 137, 143, 262, 354, 402, 422
AsuC2I CCSGG 4 cut(s) 260, 261, 406, 718
AsuHPI GGTGA 2 cut(s) 75, 905
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 2 cut(s) 354, 402
BaeGI GKGCMC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 276, 339
BauI CACGAG 2 cut(s) 275, 325
BbsI GAAGAC 3 cut(s) 169, 768, 858
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 6 cut(s) 101, 104, 107, 443, 588, 702
BccI CCATC 2 cut(s) 263, 594
BceAI ACGGC 2 cut(s) 436, 779
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 6 cut(s) 135, 141, 150, 446, 476, 947
BcnI CCSGG 4 cut(s) 260, 261, 406, 718
BcoDI GTCTC 1 cut(s) 753
BfaI CTAG 2 cut(s) 933, 939
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 755
Bme18I GGWCC 2 cut(s) 354, 402
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 6 cut(s) 137, 143, 262, 354, 402, 422
BmiI GGNNCC 2 cut(s) 404, 922
BmsI GCATC 1 cut(s) 431
BoxI GACNNNNGTC 1 cut(s) 303
BpiI GAAGAC 3 cut(s) 169, 768, 858
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpuEI CTTGAG 1 cut(s) 870
BpuMI CCSGG 4 cut(s) 260, 261, 406, 718
BsaHI GRCGYC 2 cut(s) 206, 300
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 451, 502, 812
Bse118I RCCGGY 4 cut(s) 105, 387, 399, 654
Bse1I ACTGG 1 cut(s) 33
BseBI CCWGG 6 cut(s) 135, 141, 150, 446, 476, 947
BseGI GGATG 2 cut(s) 255, 379
BseLI CCNNNNNNNGG 4 cut(s) 140, 451, 502, 812
BseMII CTCAG 2 cut(s) 569, 680
BseNI ACTGG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 157
BseX3I CGGCCG 2 cut(s) 103, 704
BseXI GCAGC 6 cut(s) 101, 104, 107, 443, 588, 702
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 595
Bsh1236I CGCG 4 cut(s) 51, 188, 200, 808
Bsh1285I CGRYCG 2 cut(s) 106, 707
BsiEI CGRYCG 2 cut(s) 106, 707
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 259
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 4 cut(s) 140, 451, 502, 812
BsmAI GTCTC 1 cut(s) 753
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 157, 276, 339
Bsp143I GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
BspCNI CTCAG 2 cut(s) 568, 679
BspFNI CGCG 4 cut(s) 51, 188, 200, 808
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 404, 922
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 755
BspPI GGATC 2 cut(s) 277, 338
BsrFI RCCGGY 4 cut(s) 105, 387, 399, 654
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 4 cut(s) 105, 387, 399, 654
BssMI GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
BssNI GRCGYC 2 cut(s) 206, 300
BssSI CACGAG 2 cut(s) 275, 325
BssT1I CCWWGG 2 cut(s) 833, 924
Bst2BI CACGAG 2 cut(s) 275, 325
Bst2UI CCWGG 6 cut(s) 135, 141, 150, 446, 476, 947
Bst4CI ACNGT 3 cut(s) 535, 650, 790
Bst6I CTCTTC 2 cut(s) 167, 623
BstACI GRCGYC 2 cut(s) 206, 300
BstAPI GCANNNNNTGC 1 cut(s) 761
BstC8I GCNNGC 6 cut(s) 107, 184, 312, 350, 411, 656
BstDEI CTNAG 3 cut(s) 555, 666, 963
BstDSI CCRYGG 2 cut(s) 268, 418
BstF5I GGATG 2 cut(s) 255, 379
BstFNI CGCG 4 cut(s) 51, 188, 200, 808
BstHHI GCGC 4 cut(s) 15, 188, 202, 214
BstKTI GATC 7 cut(s) 6, 49, 285, 333, 429, 469, 643
BstMAI GTCTC 1 cut(s) 753
BstMBI GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
BstMCI CGRYCG 2 cut(s) 106, 707
BstMWI GCNNNNNNNGC 5 cut(s) 86, 95, 111, 320, 761
BstNI CCWGG 6 cut(s) 135, 141, 150, 446, 476, 947
BstPAI GACNNNNGTC 1 cut(s) 303
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 4 cut(s) 51, 188, 200, 808
BstV1I GCAGC 6 cut(s) 101, 104, 107, 443, 588, 702
BstV2I GAAGAC 3 cut(s) 169, 768, 858
BstZI CGGCCG 2 cut(s) 103, 704
BtgI CCRYGG 2 cut(s) 268, 418
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 2 cut(s) 255, 379
BtsI GCAGTG 2 cut(s) 116, 578
BtsIMutI CAGTG 2 cut(s) 116, 578
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 6 cut(s) 107, 184, 312, 350, 411, 656
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 381
CfoI GCGC 4 cut(s) 15, 188, 202, 214
Cfr10I RCCGGY 4 cut(s) 105, 387, 399, 654
Cfr13I GGNCC 6 cut(s) 137, 143, 262, 354, 402, 422
Cfr9I CCCGGG 1 cut(s) 259
CseI GACGC 2 cut(s) 163, 177
CsiI ACCWGGT 1 cut(s) 148
Csp6I GTAC 1 cut(s) 714
CviAII CATG 1 cut(s) 382
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 3 cut(s) 555, 666, 963
DpnI GATC 7 cut(s) 5, 48, 284, 332, 428, 468, 642
DpnII GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
DraIII CACNNNGTG 2 cut(s) 152, 235
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 5 cut(s) 103, 407, 636, 704, 792
EagI CGGCCG 2 cut(s) 103, 704
Eam1104I CTCTTC 2 cut(s) 167, 623
EarI CTCTTC 2 cut(s) 167, 623
EciI GGCGGA 2 cut(s) 297, 367
EclXI CGGCCG 2 cut(s) 103, 704
Eco130I CCWWGG 2 cut(s) 833, 924
Eco24I GRGCYC 2 cut(s) 276, 339
Eco47I GGWCC 2 cut(s) 354, 402
Eco52I CGGCCG 2 cut(s) 103, 704
Eco57I CTGAAG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 721
EcoRII CCWGG 6 cut(s) 133, 139, 148, 444, 474, 945
EcoT14I CCWWGG 2 cut(s) 833, 924
EcoT38I GRGCYC 2 cut(s) 276, 339
ErhI CCWWGG 2 cut(s) 833, 924
FaeI CATG 1 cut(s) 385
FaiI YATR 1 cut(s) 383
FalI AAGNNNNNCTT 1 cut(s) 946
FaqI GGGAC 1 cut(s) 388
FatI CATG 1 cut(s) 381
FauI CCCGC 2 cut(s) 319, 341
FokI GGATG 2 cut(s) 242, 386
FriOI GRGCYC 2 cut(s) 276, 339
FspBI CTAG 2 cut(s) 933, 939
FspI TGCGCA 1 cut(s) 14
GlaI GCGC 4 cut(s) 14, 187, 201, 213
GsaI CCCAGC 1 cut(s) 174
HgaI GACGC 2 cut(s) 163, 177
HhaI GCGC 4 cut(s) 15, 188, 202, 214
Hin1I GRCGYC 2 cut(s) 206, 300
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 4 cut(s) 13, 186, 200, 212
HinP1I GCGC 4 cut(s) 13, 186, 200, 212
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 4 cut(s) 553, 561, 582, 758
HphI GGTGA 2 cut(s) 75, 905
Hpy166II GTNNAC 2 cut(s) 155, 552
Hpy188I TCNGA 2 cut(s) 370, 733
Hpy188III TCNNGA 2 cut(s) 382, 680
Hpy8I GTNNAC 2 cut(s) 155, 552
Hpy99I CGWCG 3 cut(s) 193, 208, 654
HpyAV CCTTC 3 cut(s) 65, 75, 944
HpyCH4III ACNGT 3 cut(s) 535, 650, 790
HpyCH4IV ACGT 2 cut(s) 206, 300
HpyCH4V TGCA 4 cut(s) 114, 155, 576, 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 95, 111, 320, 761
HpyF3I CTNAG 3 cut(s) 555, 666, 963
HpySE526I ACGT 2 cut(s) 206, 300
Hsp92I GRCGYC 2 cut(s) 206, 300
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 4 cut(s) 13, 186, 200, 212
KroI GCCGGC 2 cut(s) 105, 654
KroNI GCCGGC 2 cut(s) 107, 656
Kzo9I GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
Lsp1109I GCAGC 6 cut(s) 101, 104, 107, 443, 588, 702
LweI GCATC 1 cut(s) 431
MabI ACCWGGT 1 cut(s) 148
MaeI CTAG 2 cut(s) 933, 939
MaeII ACGT 2 cut(s) 206, 300
MaeIII GTNAC 2 cut(s) 739, 784
MalI GATC 7 cut(s) 5, 48, 284, 332, 428, 468, 642
MboI GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
MboII GAAGA 7 cut(s) 154, 169, 581, 640, 768, 849, 858
MhlI GDGCHC 3 cut(s) 157, 276, 339
MluCI AATT 1 cut(s) 721
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 1 cut(s) 798
MreI CGCCGGCG 1 cut(s) 654
MroNI GCCGGC 2 cut(s) 105, 654
MseI TTAA 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 703
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 6 cut(s) 135, 141, 150, 446, 476, 947
MvnI CGCG 4 cut(s) 51, 188, 200, 808
MwoI GCNNNNNNNGC 5 cut(s) 86, 95, 111, 320, 761
NaeI GCCGGC 2 cut(s) 107, 656
NciI CCSGG 4 cut(s) 260, 261, 406, 718
NdeII GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
NgoMIV GCCGGC 2 cut(s) 105, 654
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 404, 922
NmeAIII GCCGAG 2 cut(s) 324, 475
NmuCI GTSAC 1 cut(s) 784
NsbI TGCGCA 1 cut(s) 14
PagI TCATGA 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 114
PdiI GCCGGC 2 cut(s) 107, 656
PfeI GAWTC 3 cut(s) 561, 582, 758
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PshAI GACNNNNGTC 1 cut(s) 303
Psp6I CCWGG 6 cut(s) 133, 139, 148, 444, 474, 945
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 6 cut(s) 133, 139, 148, 444, 474, 945
PspN4I GGNNCC 2 cut(s) 404, 922
PspPI GGNCC 6 cut(s) 137, 143, 262, 354, 402, 422
PsrI GAACNNNNNNTAC 2 cut(s) 377, 409
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 761
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
SaqAI TTAA 1 cut(s) 915
Sau3AI GATC 7 cut(s) 3, 46, 282, 330, 426, 466, 640
Sau96I GGNCC 6 cut(s) 137, 143, 262, 354, 402, 422
SchI GAGTC 1 cut(s) 547
SduI GDGCHC 3 cut(s) 157, 276, 339
SexAI ACCWGGT 1 cut(s) 148
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 1 cut(s) 762
SgrAI CRCCGGYG 1 cut(s) 654
SinI GGWCC 2 cut(s) 354, 402
SmaI CCCGGG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 1 cut(s) 721
SspMI CTAG 2 cut(s) 933, 939
StyI CCWWGG 2 cut(s) 833, 924
TaaI ACNGT 3 cut(s) 535, 650, 790
TaiI ACGT 2 cut(s) 209, 303
TaqI TCGA 2 cut(s) 165, 246
TasI AATT 1 cut(s) 721
TauI GCSGC 5 cut(s) 54, 498, 706, 805, 808
TfiI GAWTC 3 cut(s) 561, 582, 758
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 2 cut(s) 116, 578
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 6 cut(s) 89, 92, 95, 456, 576, 690
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 4 cut(s) 38, 370, 398, 898
TspGWI ACGGA 2 cut(s) 223, 624
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 2 cut(s) 116, 578
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 2 cut(s) 354, 402
XapI RAATTY 1 cut(s) 721
XmaI CCCGGG 1 cut(s) 259
XspI CTAG 2 cut(s) 933, 939
ZraI GACGTC 2 cut(s) 207, 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.