Rroxscaffold_4G00308410

K homology RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009714
Physical Location & Seq
Reverse (-)
29967330 .. 29971134
3805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308410.1

Sequence Viewer

Length: 978 bp
ATGGCGGAGCCCGGCCAAAAGCCCATATACCCCCAACAAATCCAAATCCAAATGCCACCCACCTTCATACCCATACCCGCCTCCCAACCCGCGCACCTGCCCTCCATGAAGCGCCGCCGCGACGACGATTTTCCGCCGATGCAAATCGGGCCGGAACCCCTGGACCCAGCCCCAAAGCGCCGGGCCATGGCCCAAGACGTCATCTTCAGAGTCGTGGTCCCGTCCAGACAGATCGGCAAGGTCATCGGCAAAGAAGGCTGCCGAATCCAGAAGATTCGTGAAGACTCCAAGGCCACCATCAAAATCGCCGACGCCATAGCTAGGCACGAGGAGCGTGTGATTATAATAAGCTCAAAGGATGGTGATGAAGATACTACTGATGCCGAGAATGCGCTGCAGCAAATAGCTGGCTTAATTCTCAAGGATGATGAGAATAGTGCTGATGCGGCAAAAGTTGGTGCGGGACATGTAGCTGCGAATACGATAAGACTTTTGATTGCTGGTTCCCAAGCAGGTTCCTTAATTGGGATGTCTGGTCAGAATATTGAGAAACTGAGGAACTCCTCTGGGGCCACAATTACAATCCTTGCTCCAAGCCAGTTGCCTTTGTGTGCTTCTGCGCATGAATCTGATCGAGTGGTACAGATATCAGGTGATGTCCCTGCTGTTCAGAAAGGTCTGGAGGAGATTGGTTGTCAACTAAGGACAACCAATCTTGCAGCTGAATATATAACTTTCGAGATGTTGATATCGGAAACAATGGTTGGTGGGTTGATTGGTAGATGTGGCTCCAACATTTCAAGGATTAGAAATGAGTCTGGCGCAATGATCAAGGTTTATGGTGGGAAAGGTGAACAAAAGCATAGGCAAATTCAGTTTTGTGGTAATGCTCAGCAGGTGGCATTGGCAAAGCAGCGGGTTGATGAGTACATTTACTCTCAGTTGATACAACAAGCTGGTGCTCAACAGACGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

325

Amino Acids

35.13

Weight (kDa)

7.64

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 69 - 121 1.5e-11 KH domain
KH_1 PF00013 161 - 230 1.5e-12 KH domain
KH_1 PF00013 245 - 308 2.4e-13 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
AarI CACCTGC 2 cut(s) 105, 886
AatII GACGTC 1 cut(s) 201
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 3 cut(s) 105, 503, 886
AccII CGCG 2 cut(s) 92, 120
AciI CCGC 9 cut(s) 5, 78, 90, 115, 118, 134, 446, 461, 916
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 870
AcuI CTGAAG 1 cut(s) 190
AcyI GRCGYC 2 cut(s) 198, 312
AfaI GTAC 2 cut(s) 642, 929
AfiI CCNNNNNNNGG 3 cut(s) 187, 321, 525
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 1 cut(s) 801
AjnI CCWGG 1 cut(s) 159
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AluBI AGCT 6 cut(s) 320, 351, 407, 473, 722, 956
AluI AGCT 6 cut(s) 320, 351, 407, 473, 722, 956
Alw21I GWGCWC 1 cut(s) 964
AoxI GGCC 6 cut(s) 13, 149, 183, 189, 291, 570
ApeKI GCWGC 6 cut(s) 258, 394, 397, 473, 719, 913
ApoI RAATTY 1 cut(s) 870
AspLEI GCGC 6 cut(s) 94, 114, 180, 394, 622, 824
AspS9I GGNCC 6 cut(s) 149, 163, 183, 190, 217, 570
AsuC2I CCSGG 2 cut(s) 12, 182
AsuHPI GGTGA 3 cut(s) 374, 665, 863
AvaII GGWCC 2 cut(s) 163, 217
BanII GRGCYC 1 cut(s) 12
BauI CACGAG 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 964
BbvI GCAGC 6 cut(s) 245, 381, 409, 460, 731, 925
BccI CCATC 2 cut(s) 305, 353
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 2 cut(s) 12, 182
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 395
BfoI RGCGCY 2 cut(s) 115, 181
BfuAI ACCTGC 3 cut(s) 105, 503, 886
BisI GCNGC 9 cut(s) 115, 118, 259, 395, 398, 447, 474, 720, 914
BlpI GCTNAGC 1 cut(s) 891
BlsI GCNGC 9 cut(s) 116, 119, 260, 396, 399, 448, 475, 721, 915
Bme1390I CCNGG 3 cut(s) 12, 161, 182
Bme18I GGWCC 2 cut(s) 163, 217
BmgT120I GGNCC 6 cut(s) 149, 163, 183, 190, 217, 570
BmiI GGNNCC 8 cut(s) 9, 156, 165, 219, 505, 517, 571, 790
BmrFI CCNGG 3 cut(s) 12, 161, 182
BmsI GCATC 3 cut(s) 129, 370, 433
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 701
Bpu1102I GCTNAGC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 12, 182
BsaBI GATNNNNATC 1 cut(s) 143
BsaHI GRCGYC 2 cut(s) 198, 312
BsaJI CCNNGG 3 cut(s) 159, 186, 288
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 321, 525
Bse1I ACTGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 143
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 159, 186, 288
BseGI GGATG 3 cut(s) 364, 430, 534
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 3 cut(s) 187, 321, 525
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 3 cut(s) 545, 905, 953
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 3 cut(s) 344, 553, 698
BseXI GCAGC 6 cut(s) 245, 381, 409, 460, 731, 925
BseYI CCCAGC 1 cut(s) 166
Bsh1236I CGCG 2 cut(s) 92, 120
BshFI GGCC 6 cut(s) 15, 151, 185, 191, 293, 572
BsiHKAI GWGCWC 1 cut(s) 964
BsiSI CCGG 3 cut(s) 12, 152, 181
BslFI GGGAC 3 cut(s) 203, 477, 644
BslI CCNNNNNNNGG 3 cut(s) 187, 321, 525
BsmFI GGGAC 3 cut(s) 203, 477, 644
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 6 cut(s) 15, 151, 185, 191, 293, 572
Bsp1286I GDGCHC 2 cut(s) 12, 964
Bsp143I GATC 3 cut(s) 231, 631, 828
Bsp1720I GCTNAGC 1 cut(s) 891
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 9 cut(s) 5, 78, 90, 115, 118, 134, 446, 461, 916
BspANI GGCC 6 cut(s) 15, 151, 185, 191, 293, 572
BspCNI CTCAG 3 cut(s) 546, 904, 952
BspFNI CGCG 2 cut(s) 92, 120
BspLI GGNNCC 8 cut(s) 9, 156, 165, 219, 505, 517, 571, 790
BspMAI CTGCAG 1 cut(s) 399
BspMI ACCTGC 3 cut(s) 105, 503, 886
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 159, 186, 288
BssMI GATC 3 cut(s) 231, 631, 828
BssNI GRCGYC 2 cut(s) 198, 312
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 2 cut(s) 186, 288
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 161
BstACI GRCGYC 2 cut(s) 198, 312
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 4 cut(s) 554, 701, 891, 939
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 364, 430, 534
BstFNI CGCG 2 cut(s) 92, 120
BstH2I RGCGCY 2 cut(s) 115, 181
BstHHI GCGC 6 cut(s) 94, 114, 180, 394, 622, 824
BstKTI GATC 3 cut(s) 234, 634, 831
BstMBI GATC 3 cut(s) 231, 631, 828
BstMWI GCNNNNNNNGC 5 cut(s) 148, 255, 331, 389, 446
BstNI CCWGG 1 cut(s) 161
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 10, 159, 180
BstSFI CTRYAG 1 cut(s) 395
BstUI CGCG 2 cut(s) 92, 120
BstV1I GCAGC 6 cut(s) 245, 381, 409, 460, 731, 925
BstV2I GAAGAC 1 cut(s) 288
BsuRI GGCC 6 cut(s) 15, 151, 185, 191, 293, 572
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 3 cut(s) 364, 430, 534
BveI ACCTGC 3 cut(s) 105, 503, 886
Cac8I GCNNGC 1 cut(s) 409
CfoI GCGC 6 cut(s) 94, 114, 180, 394, 622, 824
Cfr13I GGNCC 6 cut(s) 149, 163, 183, 190, 217, 570
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 641, 928
CviAII CATG 4 cut(s) 106, 187, 467, 623
CviQI GTAC 2 cut(s) 641, 928
DdeI CTNAG 4 cut(s) 554, 701, 891, 939
DpnI GATC 3 cut(s) 233, 633, 830
DpnII GATC 3 cut(s) 231, 631, 828
EaeI YGGCCR 1 cut(s) 13
EciI GGCGGA 2 cut(s) 20, 123
Eco130I CCWWGG 2 cut(s) 186, 288
Eco24I GRGCYC 1 cut(s) 12
Eco32I GATATC 2 cut(s) 648, 750
Eco47I GGWCC 2 cut(s) 163, 217
Eco57I CTGAAG 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 159
EcoRV GATATC 2 cut(s) 648, 750
EcoT14I CCWWGG 2 cut(s) 186, 288
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 186, 288
FaeI CATG 4 cut(s) 109, 190, 470, 626
FaqI GGGAC 3 cut(s) 203, 477, 644
FatI CATG 4 cut(s) 105, 186, 466, 622
FauI CCCGC 4 cut(s) 85, 97, 454, 909
FbaI TGATCA 1 cut(s) 828
Fnu4HI GCNGC 9 cut(s) 115, 118, 259, 395, 398, 447, 474, 720, 914
FokI GGATG 3 cut(s) 371, 437, 541
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 9 cut(s) 115, 118, 259, 395, 398, 447, 474, 720, 914
FspBI CTAG 1 cut(s) 321
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 6 cut(s) 93, 113, 179, 393, 621, 823
GluI GCNGC 9 cut(s) 115, 118, 259, 395, 398, 447, 474, 720, 914
GsaI CCCAGC 1 cut(s) 170
GsuI CTGGAG 1 cut(s) 701
HaeII RGCGCY 2 cut(s) 115, 181
HaeIII GGCC 6 cut(s) 15, 151, 185, 191, 293, 572
HapII CCGG 3 cut(s) 12, 152, 181
HgaI GACGC 1 cut(s) 320
HhaI GCGC 6 cut(s) 94, 114, 180, 394, 622, 824
Hin1I GRCGYC 2 cut(s) 198, 312
Hin1II CATG 4 cut(s) 109, 190, 470, 626
Hin6I GCGC 6 cut(s) 92, 112, 178, 392, 620, 822
HinP1I GCGC 6 cut(s) 92, 112, 178, 392, 620, 822
HincII GTYRAC 1 cut(s) 698
HindII GTYRAC 1 cut(s) 698
HinfI GANTC 6 cut(s) 210, 264, 274, 284, 626, 815
HpaII CCGG 3 cut(s) 12, 152, 181
HphI GGTGA 3 cut(s) 374, 665, 863
Hpy166II GTNNAC 2 cut(s) 698, 854
Hpy188I TCNGA 5 cut(s) 209, 540, 631, 672, 754
Hpy188III TCNNGA 5 cut(s) 225, 268, 278, 680, 739
Hpy8I GTNNAC 2 cut(s) 698, 854
Hpy99I CGWCG 3 cut(s) 125, 128, 314
HpyAV CCTTC 2 cut(s) 73, 248
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 3 cut(s) 142, 397, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 255, 331, 389, 446
HpyF3I CTNAG 4 cut(s) 554, 701, 891, 939
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 2 cut(s) 198, 312
Hsp92II CATG 4 cut(s) 109, 190, 470, 626
HspAI GCGC 6 cut(s) 92, 112, 178, 392, 620, 822
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 3 cut(s) 231, 631, 828
LmnI GCTCC 4 cut(s) 7, 331, 595, 794
Lsp1109I GCAGC 6 cut(s) 245, 381, 409, 460, 731, 925
LweI GCATC 3 cut(s) 129, 370, 433
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 198
MalI GATC 3 cut(s) 233, 633, 830
MboI GATC 3 cut(s) 231, 631, 828
MboII GAAGA 4 cut(s) 196, 283, 293, 380
MhlI GDGCHC 2 cut(s) 12, 964
MluCI AATT 4 cut(s) 414, 522, 576, 870
MlyI GAGTC 3 cut(s) 219, 278, 824
MmeI TCCRAC 1 cut(s) 816
MnlI CCTC 6 cut(s) 91, 112, 322, 549, 574, 676
MseI TTAA 2 cut(s) 413, 521
MspA1I CMGCKG 2 cut(s) 722, 916
MspI CCGG 3 cut(s) 12, 152, 181
MspR9I CCNGG 3 cut(s) 12, 161, 182
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 2 cut(s) 92, 120
MwoI GCNNNNNNNGC 5 cut(s) 148, 255, 331, 389, 446
NciI CCSGG 2 cut(s) 12, 182
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 231, 631, 828
NlaIII CATG 4 cut(s) 109, 190, 470, 626
NlaIV GGNNCC 8 cut(s) 9, 156, 165, 219, 505, 517, 571, 790
NmeAIII GCCGAG 1 cut(s) 409
NsbI TGCGCA 1 cut(s) 621
NspI RCATGY 1 cut(s) 470
PaqCI CACCTGC 2 cut(s) 105, 886
PciI ACATGT 1 cut(s) 466
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 3 cut(s) 264, 274, 626
PkrI GCNGC 9 cut(s) 116, 119, 260, 396, 399, 448, 475, 721, 915
PleI GAGTC 3 cut(s) 218, 278, 823
PpsI GAGTC 3 cut(s) 218, 278, 823
PscI ACATGT 1 cut(s) 466
PsiI TTATAA 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 8 cut(s) 9, 156, 165, 219, 505, 517, 571, 790
PspPI GGNCC 6 cut(s) 149, 163, 183, 190, 217, 570
PstI CTGCAG 1 cut(s) 399
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 2 cut(s) 642, 929
RsaNI GTAC 2 cut(s) 641, 928
SaqAI TTAA 2 cut(s) 413, 521
SatI GCNGC 9 cut(s) 115, 118, 259, 395, 398, 447, 474, 720, 914
Sau3AI GATC 3 cut(s) 231, 631, 828
Sau96I GGNCC 6 cut(s) 149, 163, 183, 190, 217, 570
SchI GAGTC 3 cut(s) 219, 278, 824
ScrFI CCNGG 3 cut(s) 12, 161, 182
SduI GDGCHC 2 cut(s) 12, 964
SfaNI GCATC 3 cut(s) 129, 370, 433
SfcI CTRYAG 1 cut(s) 395
SinI GGWCC 2 cut(s) 163, 217
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 4 cut(s) 414, 522, 576, 870
SsiI CCGC 9 cut(s) 5, 78, 90, 115, 118, 134, 446, 461, 916
SspI AATATT 1 cut(s) 544
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 10, 159, 180
StyI CCWWGG 2 cut(s) 186, 288
TaiI ACGT 1 cut(s) 201
TaqI TCGA 2 cut(s) 634, 738
TasI AATT 4 cut(s) 414, 522, 576, 870
TatI WGTACW 1 cut(s) 927
TauI GCSGC 3 cut(s) 117, 120, 449
TfiI GAWTC 3 cut(s) 264, 274, 626
Tru1I TTAA 2 cut(s) 413, 521
Tru9I TTAA 2 cut(s) 413, 521
TseI GCWGC 6 cut(s) 258, 394, 397, 473, 719, 913
TspDTI ATGAA 4 cut(s) 55, 122, 381, 639
VpaK11BI GGWCC 2 cut(s) 163, 217
XapI RAATTY 1 cut(s) 870
XceI RCATGY 1 cut(s) 470
XspI CTAG 1 cut(s) 321
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.