Rh4BG074300

K homology RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009714
Physical Location & Seq
Forward (+)
13113436 .. 13114215
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG074300.1

Sequence Viewer

Length: 414 bp
ATGGCGAAATCCGGCCAAATGCCCATGTACCCCCAACAAATCCAAATGCCACCCACCTTTATACCCATACCCCCCTCCCAACCAGTCATGAAGCGTCGCCGCGACGATGATTTTCCGCCGATGCAAACCAGGCCGAAGCCCCTGGACCCAGCCCCAAAGAGCAGGGCTAAGGCCCAAAACGTCATCTTCAGAGTCGCGGTCCCGTCTAGACAGATCAGTAAGGTCATCGGCAAAGAAGGCTGCCGAATCCAGAAGATTCGTGAAGACTCCAAGGCCACCATCAAAATAGCTGACGCAATAGCTAGACACGAGGAGCGTGTGATTATAATAAGCTCAAAGGATAGTGATGACCAAACTACTGACGCCGAGAATGCGCTGCAGCAAATAGCTGGCTTAATTCTCAAGTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

137

Amino Acids

15.45

Weight (kDa)

9.93

Isoelectric Point (pI)

64.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 63 - 116 1e-11 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccII CGCG 2 cut(s) 102, 197
AciI CCGC 3 cut(s) 100, 116, 197
AcoI YGGCCR 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 1 cut(s) 29
AjnI CCWGG 2 cut(s) 128, 141
AluBI AGCT 4 cut(s) 290, 302, 333, 389
AluI AGCT 4 cut(s) 290, 302, 333, 389
AoxI GGCC 4 cut(s) 13, 131, 171, 273
ApeKI GCWGC 3 cut(s) 240, 376, 379
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 145, 172, 199
AvaII GGWCC 2 cut(s) 145, 199
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 227, 363, 391
BccI CCATC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 130, 143
BfaI CTAG 2 cut(s) 207, 303
BfmI CTRYAG 1 cut(s) 377
BisI GCNGC 4 cut(s) 100, 241, 377, 380
BlsI GCNGC 4 cut(s) 101, 242, 378, 381
Bme1390I CCNGG 2 cut(s) 130, 143
Bme18I GGWCC 2 cut(s) 145, 199
BmgT120I GGNCC 3 cut(s) 145, 172, 199
BmiI GGNNCC 2 cut(s) 147, 201
BmrFI CCNGG 2 cut(s) 130, 143
BmsI GCATC 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 270
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 141, 270
Bse1I ACTGG 1 cut(s) 83
BseBI CCWGG 2 cut(s) 130, 143
BseDI CCNNGG 2 cut(s) 141, 270
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 3 cut(s) 227, 363, 391
BseYI CCCAGC 1 cut(s) 148
Bsh1236I CGCG 2 cut(s) 102, 197
BshFI GGCC 4 cut(s) 15, 133, 173, 275
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 4 cut(s) 15, 133, 173, 275
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 3 cut(s) 100, 116, 197
BspANI GGCC 4 cut(s) 15, 133, 173, 275
BspFNI CGCG 2 cut(s) 102, 197
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 147, 201
BspMAI CTGCAG 1 cut(s) 381
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 141, 270
BssMI GATC 1 cut(s) 213
BssNI GRCGYC 1 cut(s) 363
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 270
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 2 cut(s) 130, 143
BstACI GRCGYC 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 168
BstFNI CGCG 2 cut(s) 102, 197
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 1 cut(s) 216
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 130, 237, 371
BstNI CCWGG 2 cut(s) 130, 143
BstSCI CCNGG 2 cut(s) 128, 141
BstSFI CTRYAG 1 cut(s) 377
BstUI CGCG 2 cut(s) 102, 197
BstV1I GCAGC 3 cut(s) 227, 363, 391
BstV2I GAAGAC 1 cut(s) 270
BsuRI GGCC 4 cut(s) 15, 133, 173, 275
Cac8I GCNNGC 1 cut(s) 391
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 145, 172, 199
CseI GACGC 3 cut(s) 83, 302, 371
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 25, 88
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 168
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
EaeI YGGCCR 1 cut(s) 13
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 145, 199
Eco57I CTGAAG 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 128, 141
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 2 cut(s) 28, 91
FaiI YATR 5 cut(s) 26, 62, 68, 89, 326
FaqI GGGAC 1 cut(s) 185
FatI CATG 2 cut(s) 24, 87
Fnu4HI GCNGC 4 cut(s) 100, 241, 377, 380
Fsp4HI GCNGC 4 cut(s) 100, 241, 377, 380
FspBI CTAG 2 cut(s) 207, 303
GlaI GCGC 1 cut(s) 375
GluI GCNGC 4 cut(s) 100, 241, 377, 380
GsaI CCCAGC 1 cut(s) 152
HaeIII GGCC 4 cut(s) 15, 133, 173, 275
HapII CCGG 1 cut(s) 12
HgaI GACGC 3 cut(s) 83, 302, 371
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 2 cut(s) 28, 91
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HinfI GANTC 4 cut(s) 192, 246, 256, 266
HpaII CCGG 1 cut(s) 12
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 4 cut(s) 88, 207, 250, 260
Hpy99I CGWCG 2 cut(s) 99, 107
HpyAV CCTTC 1 cut(s) 230
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 2 cut(s) 124, 379
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 237, 371
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 2 cut(s) 28, 91
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 3 cut(s) 227, 363, 391
LweI GCATC 1 cut(s) 111
MaeI CTAG 2 cut(s) 207, 303
MaeII ACGT 1 cut(s) 180
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 3 cut(s) 178, 265, 275
MluCI AATT 1 cut(s) 396
MlyI GAGTC 2 cut(s) 201, 260
MnlI CCTC 2 cut(s) 85, 304
MseI TTAA 1 cut(s) 395
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 2 cut(s) 130, 143
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 2 cut(s) 130, 143
MvnI CGCG 2 cut(s) 102, 197
MwoI GCNNNNNNNGC 3 cut(s) 130, 237, 371
NdeII GATC 1 cut(s) 213
NlaIII CATG 2 cut(s) 28, 91
NlaIV GGNNCC 2 cut(s) 147, 201
NmeAIII GCCGAG 1 cut(s) 391
PagI TCATGA 1 cut(s) 87
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 2 cut(s) 246, 256
PkrI GCNGC 4 cut(s) 101, 242, 378, 381
PleI GAGTC 2 cut(s) 200, 260
PpsI GAGTC 2 cut(s) 200, 260
PsiI TTATAA 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 128, 141
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 2 cut(s) 128, 141
PspN4I GGNNCC 2 cut(s) 147, 201
PspPI GGNCC 3 cut(s) 145, 172, 199
PstI CTGCAG 1 cut(s) 381
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 4 cut(s) 100, 241, 377, 380
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 3 cut(s) 145, 172, 199
SchI GAGTC 2 cut(s) 201, 260
ScrFI CCNGG 2 cut(s) 130, 143
SetI ASST 7 cut(s) 59, 183, 225, 292, 304, 335, 391
SfaNI GCATC 1 cut(s) 111
SfcI CTRYAG 1 cut(s) 377
SinI GGWCC 2 cut(s) 145, 199
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 1 cut(s) 396
SsiI CCGC 3 cut(s) 100, 116, 197
SspMI CTAG 2 cut(s) 207, 303
StyD4I CCNGG 2 cut(s) 128, 141
StyI CCWWGG 1 cut(s) 270
TaiI ACGT 1 cut(s) 183
TasI AATT 1 cut(s) 396
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 2 cut(s) 246, 256
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TseI GCWGC 3 cut(s) 240, 376, 379
TspDTI ATGAA 1 cut(s) 104
VpaK11BI GGWCC 2 cut(s) 145, 199
XbaI TCTAGA 1 cut(s) 206
XspI CTAG 2 cut(s) 207, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.