Rorug01G0228400

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014799
Physical Location & Seq
Forward (+)
32923391 .. 32926625
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0228400.1

Sequence Viewer

Length: 456 bp
ATGGCCGGAGAAGAAGAGAACAGCGAGTTCTACCTGAGGTACTACGTCGGCCACAAGGGGAAGTTCGGGCACGAGTTCTTGGAGTTCGAGTTTCGACCCGACGGGAAGCTCCGATACGCCAACAACTCCAACTACAAGAACGACACCATCATCCGCAAGGAGGTCTACGTCACCCCCGCCGTCGTCAGAGAGTGTCGCCGTATCATTACTGAGAGCGAGATCATGAAAGAGGATGATCAGAACTGGCCGGAACCTGACCGTATTGGGCGGCAGGAGCTTGAGATTGTAATGGGGAATGAGCATATCTCCTTCACTACTTCAAAGATTGGATCCCTCGTTGATGTCCAGGGCAGTAAAGATCCTGAAGGTCTTCGCATCTTTTATTATCTTGTTCAGGATCTGAAGTGCTTTGTGTTCTCTCTCATCTCGCTCCACTTCAAGATCAAGCCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

151

Amino Acids

17.78

Weight (kDa)

5.63

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mago_nashi PF02792 10 - 151 3.8e-79 Mago nashi protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AciI CCGC 3 cut(s) 154, 177, 268
AclWI GGATC 4 cut(s) 324, 337, 353, 405
AcoI YGGCCR 3 cut(s) 3, 49, 245
AcuI CTGAAG 2 cut(s) 384, 422
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 2 cut(s) 321, 439
AjnI CCWGG 1 cut(s) 345
AluBI AGCT 2 cut(s) 109, 277
AluI AGCT 2 cut(s) 109, 277
AlwI GGATC 4 cut(s) 324, 337, 353, 405
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 3 cut(s) 3, 49, 245
Asp700I GAANNNNTTC 1 cut(s) 369
AsuHPI GGTGA 1 cut(s) 163
AxyI CCTNAGG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 72
BamHI GGATCC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 362
BccI CCATC 1 cut(s) 155
BceAI ACGGC 2 cut(s) 164, 183
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 1 cut(s) 235
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 252, 331
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 362
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpuEI CTTGAG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 248
Bse21I CCTNAGG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 2 cut(s) 150, 238
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 26, 201
BseNI ACTGG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 72
BshFI GGCC 3 cut(s) 5, 51, 247
BsiSI CCGG 2 cut(s) 6, 248
BslI CCNNNNNNNGG 1 cut(s) 160
BsnI GGCC 3 cut(s) 5, 51, 247
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 6 cut(s) 219, 235, 329, 358, 397, 441
BspACI CCGC 3 cut(s) 154, 177, 268
BspANI GGCC 3 cut(s) 5, 51, 247
BspCNI CTCAG 2 cut(s) 27, 202
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 252, 331
BspPI GGATC 4 cut(s) 324, 337, 353, 405
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 6 cut(s) 219, 235, 329, 358, 397, 441
BssSI CACGAG 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 35, 210
BstF5I GGATG 2 cut(s) 150, 238
BstKTI GATC 6 cut(s) 222, 238, 332, 361, 400, 444
BstMBI GATC 6 cut(s) 219, 235, 329, 358, 397, 441
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 362
BstX2I RGATCY 3 cut(s) 329, 358, 397
BstYI RGATCY 3 cut(s) 329, 358, 397
Bsu36I CCTNAGG 1 cut(s) 35
BsuRI GGCC 3 cut(s) 5, 51, 247
BtsCI GGATG 2 cut(s) 150, 238
CaiI CAGNNNCTG 1 cut(s) 400
CciI TCATGA 1 cut(s) 222
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 223
CviJI RGCY 6 cut(s) 5, 51, 109, 247, 277, 448
CviKI_1 RGCY 6 cut(s) 5, 51, 109, 247, 277, 448
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 35, 210
DpnI GATC 6 cut(s) 221, 237, 331, 360, 399, 443
DpnII GATC 6 cut(s) 219, 235, 329, 358, 397, 441
EaeI YGGCCR 3 cut(s) 3, 49, 245
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 384, 422
Eco81I CCTNAGG 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 1 cut(s) 226
FaiI YATR 4 cut(s) 224, 303, 452, 454
FatI CATG 1 cut(s) 222
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 137, 245
Fsp4HI GCNGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 3 cut(s) 5, 51, 247
HapII CCGG 2 cut(s) 6, 248
Hin1II CATG 1 cut(s) 226
HpaII CCGG 2 cut(s) 6, 248
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 4 cut(s) 113, 188, 240, 402
Hpy188III TCNNGA 4 cut(s) 223, 362, 395, 439
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 3 cut(s) 50, 104, 185
HpyAV CCTTC 2 cut(s) 319, 359
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 2 cut(s) 45, 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 2 cut(s) 35, 210
HpySE526I ACGT 2 cut(s) 45, 168
Hsp92II CATG 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 6 cut(s) 219, 235, 329, 358, 397, 441
LmnI GCTCC 3 cut(s) 114, 274, 435
LweI GCATC 1 cut(s) 384
MaeII ACGT 2 cut(s) 45, 168
MaeIII GTNAC 1 cut(s) 169
MalI GATC 6 cut(s) 221, 237, 331, 360, 399, 443
MboI GATC 6 cut(s) 219, 235, 329, 358, 397, 441
MboII GAAGA 3 cut(s) 23, 26, 362
MflI RGATCY 3 cut(s) 329, 358, 397
MhlI GDGCHC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 30, 154, 223, 344
MroXI GAANNNNTTC 1 cut(s) 369
MspI CCGG 2 cut(s) 6, 248
MspR9I CCNGG 1 cut(s) 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 274
NdeII GATC 6 cut(s) 219, 235, 329, 358, 397, 441
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 252, 331
NmuCI GTSAC 1 cut(s) 169
PagI TCATGA 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 369
PkrI GCNGC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 252, 331
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 3 cut(s) 329, 358, 397
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 6 cut(s) 219, 235, 329, 358, 397, 441
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 72
SetI ASST 9 cut(s) 36, 41, 48, 111, 165, 171, 256, 279, 370
SfaNI GCATC 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SsiI CCGC 3 cut(s) 154, 177, 268
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 2 cut(s) 48, 171
TaqI TCGA 2 cut(s) 87, 94
TauI GCSGC 1 cut(s) 271
TseFI GTSAC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.